Three-year lifecycle,large body,and very high threshold temperature in the cricket <Emphasis Type="Italic">Gryllus argenteus</Emphasis> for special adaptation to desiccation cycle in Malawi

In temperate climates, the initiation and termination of diapause synchronize the stress-tolerant stage with the stressful season and reproduction with the non-stressful season in many insects. Synchronization is often regulated by photoperiodism.Voltinism and the ultimate size of adults are also important determinants for their lifecycle, and different diapause stages and voltinism patterns are known in crickets.Here, we investigated the life history of the African cricket Gryllus argenteus from Malawi, which is a typical arid tropical highland. The climate is characterized by alternating arid and wet seasons, each of which lasts for half a year, and where the available heat mass is much less than lowlands at the same latitude. We first measured the nymphal duration at each rearing temperature and calculated the lower developmental threshold (t ₀) to be 20.19 °C based on Ikemoto and Takai (2000) and 19.38 °C based on a conventional line-fitting method. These values are very high relative to many other insects. The local temperature in winter does not fall below 15 °C, but this is much higher than the lethal limit. This suggested that critical stress in this locality was not coldness but low precipitation in winter. We estimated, based both on local temperature change and the Ikemoto and Takai’s t ₀, that G. argenteus required 3 years to complete its lifecycle unlike wet lowland species, where univoltinism or multi-voltinism are commonplace. Photoperiodism was observed in this species, but due to a lag between annual cycles in photoperiod, temperature, and humidity, photoperiodism alone cannot atune their lifecycle with local conditions.Synchronization in this species was achieved by three different adaptations: photoperiodism, high t ₀, and large body size, which give it a long lifecycle. Although the species cannot achieve a univoltine lifecycle because of its high t₀ value, it can escape from dry season by entering diapause at moderate temperatures, probably thereby achieving adaptive synchrony of lifecycle with both favorable and unfavorable seasons. A comparison between a conventional photothermogram and a newly formulated photohydrogram or photohygrogram demonstrates that even though sufficient heat is available, scarcity of water and thus scarcity of foliage should force the cricket to maintain diapause at intermediate temperature. The results suggested that high t ₀, large body size, and multi-ennial lifecycle mutually affect each other and formulate a unique adaptation under such an extreme environment.     

Zinc removal from synthetic waters using magnetite/graphene oxide composites

The removal of heavy metals from wastewater has become a global challenge, which demands the continuous study of efficient and low-cost treatment alternatives such as adsorption. In this research, the removal of zinc was evaluated using batch adsorption processes with nonconventional materials such as graphene oxide (GO), magnetite (MG), and their composites (GO:MG), formulated with three weight ratios (2:1, 1:1, and 1:2). Graphene was synthesized by the modified Marcano method, using pencil lead graphite as a precursor. MG and the composites were synthesized by chemical coprecipitation of ferrous sulfate and ferric chloride. The materials were characterized by Raman and Fourier transform infrared spectroscopies, scanning electron microscopy, X-ray diffraction, and the Brunauer–Emmett–Teller method to determine the functional groups, microstructural and morphological characteristics, and specific surface area. Batch adsorption tests were carried out to optimize the adsorbent dose and contact time with zinc solutions of 10 ppm. Zinc adsorption reached equilibrium at 2 h, with an optimal dose between 0.25 and 1.0 g/L. The maximum zinc removal efficiencies/adsorption capacities were 98.6%/165.6, 83.4%/47.6, 83.5%/21.9, 72.8%/19.9, and 82.2%/9.25 mg/g using GO, 2GO:1MG, 1GO:1MG, 1GO:2MG, and MG, respectively. Furthermore, the analysis of the isotherm and adsorption kinetics models determined that the adsorption processes using MG and the composites fit the Sips and pseudo-second-order models.     

Correction to: Keeping pace with forestry: Multi-scale conservation in a changing production forest matrix

Ambio - In the original published article, the sentence “Nevertheless, semi-natural forest remnants continue to be harvested and fragmented (Svensson et al. 2018; Jonsson et al. 2019), and...     

The Influence of Linear Elements on Plant Species Diversity of Mediterranean Rural Landscapes: Assessment of Different Indices and Statistical Approaches

This paper mainly aims to study the linear element influence on the estimation of vascular plant species diversity in five Mediterranean landscapes modeled as land cover patch mosaics. These landscapes have several core habitats and a different set of linear elements -habitat edges or ecotones, roads or railways, rivers, streams and hedgerows on farm land- whose plant composition were examined. Secondly, it aims to check plant diversity estimation in Mediterranean landscapes using parametric and non-parametric procedures, with two indices: Species richness and Shannon index.Land cover types and landscape linear elements were identified from aerial photographs. Their spatial information was processed using GIS techniques. Field plots were selected using a stratified sampling design according to relieve and tree density of each habitat type. A 50×20 m² multi-scale sampling plot was designed for the core habitats and across the main landscape linear elements. Richness and diversity of plant species were estimated by comparing the observed field data to ICE (Incidence-based Coverage Estimator) and ACE (Abundance-based Coverage Estimator) non-parametric estimators.The species density, percentage of unique species, and alpha diversity per plot were significantly higher (p < 0.05) in linear elements than in core habitats. ICE estimate of number of species was 32% higher than of ACE estimate, which did not differ significantly from the observed values. Accumulated species richness in core habitats together with linear elements, were significantly higher than those recorded only in the core habitats in all the landscapes. Conversely, Shannon diversity index did not show significant differences.     

Ultrafine particles at workplaces of a primary aluminium smelter

The number concentration and size distribution of ultrafine particles in a S?derberg and a prebake potroom of an aluminium primary smelter have been measured using a scanning mobility particle spectrometer. The particle morphology was studied by transmission electron microscopy (TEM). The study shows the existence of elevated number concentrations of ultrafine particles in both potrooms. The main source of these particles is likely to be the process of anode changing. The ultrafine particles were measured directly at the source but could also be identified as episodes of high number concentrations in the general background air. Unlike the larger particles belonging to the 50-100 nm mode, the nanoparticle mode could not be detected in the TEM indicating that they may not be stable under the applied sampling conditions and/or the high vacuum in the instrument.     

Bioaerosol sampling by a personal rotating cup sampler CIP 10-M

High concentrations of bioaerosols containing bacterial, fungal and biotoxinic matter are encountered in many workplaces, e.g. solid waste treatment plants, waste water treatment plants and sewage networks. A personal bioaerosol sampler, the CIP 10-M (M-microbiologic), has been developed to measure worker exposure to airborne biological agents. This sampler is battery operated; it is light and easy to wear and offers full work shift autonomy. It can sample much higher concentrations than biological impactors and limits the mechanical stress on the microorganisms. Biological particles are collected in 2 ml of liquid medium inside a rotating cup fitted with radial vanes to maintain an air flow rate of 10 l min(-1) at a rotational speed of approximately 7,000 rpm. The rotating cup is made of sterilisable material. The sampled particles follow a helicoidal trajectory as they are pushed to the surface of the liquid by centrifugal force, which creates a thin vertical liquid layer. Sterile water or another collecting liquid can be used. Three particle size selectors allow health-related aerosol fractions to be sampled according to international conventions. The sampled microbiological particles can be easily recovered for counting, incubation or further biochemical analysis, e.g., for airborne endotoxins. Its physical sampling efficiency was laboratory tested and field trials were carried out in industrial waste management conditions. The results indicate satisfactory collection efficiency, whilst experimental application has demonstrated the usefulness of the CIP 10-M personal sampler for individual bioaerosol exposure monitoring.     

Choosing policy instruments for controlling ozone depleting substances in a developing context: the case of Chile

Chile ratified the Montreal Protocol in 1990 which sets out an agenda for the reduction and then elimination of ozone depleting substances (ODS); however, by 1998 the country had not yet defined a strategy to encourage greater compliance, so that in the same year the National Environmental Commission decided to examine the policy options available. This paper examines the process followed to develop this strategy. As a first step it was necessary to look at how far the implicit policy of "business as usual" could be stretched without jeopardizing Chile's compliance obligations. A second step included quantifying compliance costs and their impact on different policy instruments and so policy choice. Finally, the attitudes of decision-makers or participants were identified, by interviews with officials of the different public agents involved, in order to weigh their views about policy. A strategy was proposed, based on this information, which the Chilean regulator has used, although not in its entirety. The methodology developed could well be useful for developing-country Protocol signatories building their own appropriate compliance strategy.     

Effect of Cadmium on Microbial Activity and a Ryegrass Crop in Two Semiarid Soils

Soil pollution with Cd is an environmental problem common in the world, and it is necessary to establish what Cd concentrations in soil could be dangerous to its fertility from toxicity effects and the risk of transference of this element to plants and other organisms of the food chain. In this study, we assessed Cd toxicity on soil microorganisms and plants in two semiarid soils (uncultivated and cultivated). Soil ATP content, dehydrogenase activity, and plant growth were measured in the two soils spiked with concentrations ranging from 3 to 8000 mg Cd/kg soil and incubated for 3 h, 20 days, and 60 days. The Cd concentrations that produced 5%; 10%;, and 50%; inhibition of each of the two soil microbiological parameter studied (ecological dose, ED, values) were calculated using two different mathematical models. Also, the effect of Cd concentration on plant growth of ryegrass (Lolium perenne, L.) was studied in the two soils. The Cd ED values calculated for soil dehydrogenase activity and ATP content were higher in the agricultural soils than in the bare soil. For ATP inhibition, higher ED values were calculated than for dehydrogenase activity inhibition. The average yields of ryegrass were reduced from 5.03 to 3.56 g in abandoned soil and from 4.21 to 1.15 g in agricultural soil with increasing concentrations of Cd in the soil. Plant growth was totally inhibited in abandoned and agricultural soils at Cd concentrations above 2000 and 5000 mg/kg soil, respectively. There was a positive correlation between the concentration of Cd in the plants and the total or DTPA-extractable concentrations of Cd in the soil.     

Influence of soil properties and aging on the toxicity of copper on compost worm and barley

Influence of soil properties and aging on Cu partitioning and toxicity was assessed on 10 artificial soils constituted using a statistical design considering pH (5.5 and 7.5), organic matter (1-30% [w/w]), and clay content (5-35% [w/w]). Total Cu as well as water-, CaCl2-, and diethylene triamine pentaacetic acid (DTPA)-extracted Cu fractions were determined for each soil mixture. Ecotoxic effect was assessed by determining growth inhibition of barley (Hordeum vulgare L.) and compost worm (Eisenia fetida) mortality. Analyses were repeated after a 16-wk aging period of the soils at pH 7.5 (8 x 2-wk wetting and drying cycle). Results indicated that pH was the main factor controlling Cu partitioning, ahead of organic matter and clay content. Calcium chloride (0.5 M)-extracted Cu fractions showed the best correlation with toxic responses (r = 0.55-0.66; p < 0.05), while total and DTPA-extracted Cu concentrations could not explain differences in toxicity. Direct regressions between toxicity and soil properties (pH, organic matter, and clay content) provided better explanation of variance: r2= 0.50 (p = 0.00006) for compost worm mortality, r2= 0.77 (p < 0.00001) for barley shoot inhibition, and r2= 0.92 (p < 0.00001) for barley root inhibition. Copper toxicity was mainly influenced by pH and, to a lesser extent, by organic matter and clay content. Aging in organic soils revealed a slight reduction in ecotoxicity while an increase was observed in soils with low organic matter content. Further investigation using longer aging periods would be necessary to assess the significance of this observation.     

Use of beryllium-7 to document soil redistribution following forest harvest operations

Rapid and reliable methods for documenting soil erosion associated with forest harvest operations are needed to support the development of best management practices for soil and water conservation. To address this need, the potential for using 7Be measurements to estimate patterns and amounts of soil redistribution associated with individual post-harvest events was explored. The 7Be technique, which was originally developed for use on agricultural land, was employed to estimate soil redistribution associated with a period of heavy rainfall within a harvested forest area located in the Lake Region of Chile (39 degrees 44'7' S, 73 degrees 10'39' W; 22% slope; and mean annual rainfall 2300 mm yr(-1)). The results provided by the 7Be technique were validated against direct measurements of soil gain or loss during the same period obtained using erosion pins. The information produced by the two approaches was similar. The results of this study demonstrate the potential for using 7Be measurements to document event-based erosion in recently harvested forest areas.