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71.
A woman in the 32nd week of pregnancy was referred for investigation because of fetal abnormalities, including an abdominal wall defect, detected by ultrasonography. In view of the increased risk of chromosome abnormality, amniocentesis was performed to enable informed decisions about the management of the pregnancy and delivery to be taken. Cells from the liquor were inoculated into standard lymphocyte culture medium and incubated for 72 h. Slides with a high mitotic index and good quality metaphases, comparable to those from a blood culture, were obtained after harvesting. Cytogenetic analysis showed the karyotype to be 46,XY,—14,+t(13ql4q), which is consistent with Patau's syndrome. This technique appears to be an option for rapid karyotyping in cases of abdominal wall defect, where a chromosomal abnormality is suspected.  相似文献   
72.
Prenatal diagnosis of Sandhoff disease (infantile onset) at 16 weeks gestation has been made by detection and analysis of N-acetylglucosaminyl-oligosaccharides in amniotic fluid using high performance liquid chromatography. The elution profile for the branched chain oli-gosaccharides was identical with that obtained with neonatal and infantile Sandhoff urine. The concentration of the oligosaccharides in the fluid was 1/100th that of urine but when calculated relative to creatinine the levels were similar. No oligosaccharides were detected in normal control amniotic fluids (10 patients) at a similar gestational age. Based on the levels of the amniotic fluid oligosaccharides and the sensitivity limits of the assay, prenatal diagnosis of patients with the juvenile onset form of the disease may also be possible with this technique.  相似文献   
73.
Chorionic villus sampling (CVS) with either transcervical catheters or transabdominal needles is a widely-accepted method for prenatal diagnosis. However, there exists a small subset of patients in whom sampling is difficult or impossible with either route because of individual anatomic variations. A new method of chorionic villus biopsy has been developed to circumvent these problems, utilizing transvaginal chorionic needle aspiration guided by an intravaginal ultrasound probe. This technique was performed successfully in 15 patients in whom villi could not be obtained by either of the conventional methods. This method now makes CVS possible in essentially all women regardless of their uterine anatomy or placental placement; it may also prove useful for very early chorionic sampling.  相似文献   
74.
We selected the Guanting Reservoir in Beijing, China as a case where an industrial area locates on the upwind corner to study the influence of human activities and natural processes on the distribution of polycyclic aromatic hydrocarbons (PAHs) in soils. Soil PAH concentrations in the study area follow a log-normal probability distribution function, suggesting that distribution of PAH in soils was affected by human activities. Distribution of PAHs in soils was significantly affected by the point source that high PAH concentrations were observed in near industrial area with an obvious declining trend from the northwest to the southeast which was the prevailing wind direction in this area. Away from the influence of point source, distribution of PAHs in soils was found to significantly correlate with total organic carbon content, while the influences of agricultural land uses and type of soil texture on the total soil PAHs contents and ring compositions were quite limited. The results can provide some evidences and data on the pollutant accumulation in drink water protection area influenced by natural processes and human activities.  相似文献   
75.
Life history costs of olfactory status signalling in mice   总被引:4,自引:0,他引:4  
Large body size confers a competitive advantage in animal contests but does not always determine the outcome. Here we explore the trade-off between short-term achievement of high social status and longer-term life history costs in animals which vary in competitive ability. Using laboratory mice, Mus musculus, as a model system, we show that small competitors can initially maintain dominance over larger males by increasing investment in olfactory status signalling (scent-marking), but only at the cost of reduced growth rate and body size. As a result they become more vulnerable to dominance reversals later in life. Our results also provide the first empirical information about life history costs of olfactory status signals. Received: 15 December 1999 / Revised: 6 June 2000 / Accepted: 24 June 2000  相似文献   
76.
The dynamics of arsenic in four paddy fields in the Bengal delta   总被引:4,自引:0,他引:4  
Irrigation with arsenic contaminated groundwater in the Bengal Delta may lead to As accumulation in the soil and rice grain. The dynamics of As concentration and speciation in paddy fields during dry season (boro) rice cultivation were investigated at 4 sites in Bangladesh and West Bengal, India. Three sites which were irrigated with high As groundwater had elevated As concentrations in the soils, showing a significant gradient from the irrigation inlet across the field. Arsenic concentration and speciation in soil pore water varied temporally and spatially; higher As concentrations were associated with an increasing percentage of arsenite, indicating a reductive mobilization. Concentrations of As in rice grain varied by 2-7 fold within individual fields and were poorly related with the soil As concentration. A field site employing alternating flooded-dry irrigation produced the lowest range of grain As concentration, suggesting a lower soil As availability caused by periodic aerobic conditions.  相似文献   
77.
Polyurethane foam (PUF) disks were deployed at global background sites, to test logistical issues associated with a global monitoring network for persistent organic pollutants (POPs). alpha-HCH, exhibited relatively high and uniform concentrations (17-150 pg/m3) at temperate and arctic sites with elevated concentrations associated with trans-Pacific inflow. Concentrations were much lower (<5 pg/m3) in Bermuda, Chile and Cape Grim. Concentrations for gamma-HCH, the main component of lindane, were spatially similar to the alpha-HCH pattern but lower in magnitude (typically, <10 pg/m3). Chlordane concentrations (sum of cis-chlordane, trans-chlordane and trans-nonachlor) were also low (<10 pg/m3). Dieldrin concentrations were in the range 2-25 pg/m3 at most sites but elevated in Bermuda. Back trajectories suggest that advection from Africa and the US may contribute. Endosulfan, a popular current-use pesticide, exhibited highest concentrations ranging from tens to hundreds of pg/m3. There was good agreement between duplicate samplers at each site and PUF disk-derived air concentrations agreed with high volume data. Few logistical/analytical problems were encountered in this pilot study.  相似文献   
78.
'Persistent organic pollutants' (POPs) are semi-volatile, mobile in the environment and bioaccumulate. Their toxicity and propensity for long-range atmospheric transport (LRAT) has led to international bans/restrictions on their use/release. LRAT of POPs may occur by a 'single hop' or repeated temperature-driven air-surface exchange. It has been hypothesised that this will result in global fractionation and distillation-with condensation and accumulation in polar regions. Polychlorinated biphenyls (PCBs)--industrial chemicals banned/restricted in the 1970s--provide a classic illustration of POP behaviour. A latitudinally-segmented global PCB inventory has been produced, which shows that approximately 86% of the 1.3 x 10(6) tonnes produced was used in the temperate industrial zone of the northern hemisphere. A global survey of background surface soils gives evidence for 'fractionation' of PCBs. More significantly, however, very little of the total inventory has 'made the journey' via primary emission and/or air-surface exchange and LRAT out of the heavily populated source regions, in the 70 years since PCBs were first produced. Soils generally occlude PCBs, especially soils with dynamic turnover of C/bioturbation/burial mechanisms. This limits the fraction of PCBs available for repeated air-soil exchange. The forested soils of the northern hemisphere, and other C-rich soils, appear to be playing an important role in 'protecting' the Arctic from the advective supply of POPs. Whilst investigations on POPs in remote environments are important, it is imperative that researchers also seek to better understand their release from sources, persistence in source regions, and the significant loss mechanisms/global sinks of these compounds, if they wish to predict future trends.  相似文献   
79.
Continuous data of vertical-profile salinity were analyzed for four stations located successively upriver in a macrotidal estuary, the lower St. Johns River (Northeast Florida, USA). The data analysis confirmed well-mixed salinity conditions in the river with at most 1.3 ppt of vertical variability at Dames Point (river km 20), where the main variations of salinity are along the longitudinal axis of the river. Given the well-mixed salinity conditions and dominant horizontal structure of salinity variations in the river, we present and apply a barotropic, two-dimensional modeling approach for hydrodynamic-salinity transport simulation in the lower St. Johns River. When properly forced by offshore surge, high-resolution wind fields and freshwater river inflows, the model replicated the salinity measurements remarkably well, including the separation into tidal and sub-tidal components. The data and model results show that, at times, offshore winds and surge can be more influential on longitudinal salinity variations than local winds over the river. We demonstrate the importance of using proper boundary conditions to force the model relative to the minimal sensitivity of the model to parameter adjustment of horizontal mixing and uncertainty-based perturbation of wind and inflow forcings.  相似文献   
80.
An intensive field study was conducted in Research Triangle Park, North Carolina in the fall of 1986. Ambient concentrations of the following constituents were obtained: nitric acid, nitrous acid, nitrogen dioxide, sulfur dioxide, ammonia, hydrogen ion, and particulate nitrate, sulfate, and ammonium. Results collected using the annular denuder system (ADS) and the transition flow reactor (TFR) are presented and compared.

Both types of samplers had operational detection limits on daily (22-hour) samples that were generally below 1 μg m-3 suggesting that both samplers can provide sensitive measurements for most of the constituents of interest. Both the ADS and TFR show reasonable (>25 percent) within-sampler precision for most of the measured species concentrations, except TFR fine particulate nitrate measurements where results were frequently negative (The TFR fine particulate nitrate measurement is calculated using subtraction of positive numbers).

Comparison of ADS and TFR daily results showed good agreement for total particulate sulfate, the sum of total (coarse plus fine) particulate and gaseous nitrate, and ammonia. As a result of different inlet particle collection efficiencies, the ADS fine particulate sulfate exceeded the TFR (5 percent). In the absence of a filter to collect volatilized particulate ammonium in the ADS, the sum of total particulate and gaseous ammonium in the TFR exceeded that in the ADS. Of potentially more importance, ADS measurements of SO2 and H+ exceeded those of the TFR, while TFR measurements of HNO3 exceeded those of the ADS. Results of this study suggest that the TFR may provide biased measurements of SO2, H+, HNO3, and Fine NO3 - that cannot be corrected without modifications to the fundamental design of the sampling system.  相似文献   
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