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101.
102.
J?rg R?mbke Anja Coors Kathrin Fenner John Jensen Hans L?kke Christoph Sch?fers Jürg Oliver Straub Chris Metcalfe 《Environmental Sciences Europe》2012,24(1):1-7
Background
The bioaccumulation potential of chemical substances is commonly determined in flow-through fish tests according to the Organization for Economic Cooperation and Development (OECD) technical guideline (TG) 305, aiming at the determination of bioconcentration factors (BCF). Investigations on aquatic organisms have shown a significant positive correlation between the accumulation of a chemical and the lipid content of organisms. Residue levels can thus differ between individuals, species, and size groups depending on their lipid content. BCF values can be normalized by lipid content; however, this requires the use of appropriate extraction techniques to ensure the complete extraction of total lipids from fish/tissue samples. The OECD TG 305 is currently under revision. A literature review was carried out to select preferred methods which shall be included in the updated OECD TG 305 along with a sampling schedule for lipid measurement in fish.Results
An overview and appraisal of a broad range of lipid extraction methods is presented. Extraction procedures applied in bioaccumulation studies described in the literature and procedures currently used for such studies by governmental, academic, and industrial labs are surveyed. Possible impacts of unsuitable extraction techniques on lipid normalized BCF results were assessed. Default values for BCF normalization commonly used in bioaccumulation studies are described. The effect of differences in residue distribution over different organs and/or lipids on the normalization of BCF values is discussed.Conclusions
A broad range of procedures for lipid extraction are available. However, only the use of suitable extraction techniques guarantees the complete extraction of total lipids from fish samples in bioaccumulation studies which is required to ensure the correct lipid normalization of BCF values. Preferred methods for inclusion in the updated OECD 305 test guideline as well as a standard schedule for lipid measurements are suggested. 相似文献103.
Julia Wohlers-Z?llner Antje Biermann Anja Engel Petra D?rge Aleksandra M. Lewandowska Markus von Scheibner Ulf Riebesell 《Marine Biology》2012,159(11):2503-2518
A comparative analysis of data, obtained during four indoor-mesocosm experiments with natural spring plankton communities from the Baltic Sea, was conducted to investigate whether biogeochemical cycling is affected by an increase in water temperature of up to 6?°C above present-day conditions. In all experiments, warming stimulated in particular heterotrophic bacterial processes and had an accelerating effect on the temporal development of phytoplankton blooms. This was also mirrored in the build-up and partitioning of organic matter between particulate and dissolved phases. Thus, warming increased both the magnitude and rate of dissolved organic carbon (DOC) build-up, whereas the accumulation of particulate organic carbon (POC) and phosphorus (POP) decreased with rising temperature. In concert, the observed temperature-mediated changes in biogeochemical components suggest strong shifts in the functioning of marine pelagic food webs and the ocean’s biological carbon pump, hence providing potential feedback mechanisms to Earth’s climate system. 相似文献
104.
Anja Vespermann Frank Riepert und Jochen Pflugmacher 《Umweltwissenschaften und Schadstoff-Forschung》2003,15(3):143-151
Zusammenfassung Ziel der beschriebenen Untersuchungen war die praktische überprüfung eines Methodenentwurfs zur Erfassung der Bioakkumulation
von Umweltchemikalien mitEisenia fetida, der Eignung vonE. fetida als Modellorganismus und des OECD-Kunstbodens als Standardsubstrat. Als Testorganismen wurden aus eigener Zucht der KompostwurmEisenia fetida und nach FeldentnahmeAllolobophora caliginosa, Allolobophora chlorotica, Allolobophora longa undLumbricus rubellus eingesetzt. Testb?den waren der OECD-Kunstboden und ein Boden vom Versuchsfeld der BBA in Berlin-Dahlem. Beiden B?den wurden
die Testsubstanzen Hexachlorbenzol und Pyren in einer Konzentration von 10 mg/kg Bodentrockengewicht zugemischt. Die Expositionszeit
der Regenwürmer betrug 4 Wochen bei w?chentlicher Bestimmung der Konzentrationen der Testsubstanzen in den Würmern und B?den.
Die fürE. fetida sowie die Wildarten errechneten Anreicherungsfaktoren (AF) beliefen sich bei HCB in Abh?ngigkeit des verwendeten Bodens auf
Werte zwischen 10 und 17 und bei Pyren auf Werte zwischen 0,9 und 1,7. Durch Umrechnung der Bodenkonzentrationen auf Gehalte
in der Wasserphase und Ableitung der entsprechenden Anreicherungsfaktoren kann ein Vergleich mit BCF-Werten, die aus QSAR’S
anderer Regenwurmarten und Fischen berechnet wurden, hergestellt werden. Die Ergebnisse lassen den Schluss zu, dass der Regenwurmtest
mitE. fetida (OECD 1984, ISO 1998) für die Untersuchung der Bioakkumulation geeignet ist.
OnlineFirst: 31. 05. 2002 相似文献
105.
This study investigates the influence of the two different clay minerals kaolinite and smectite as well as of organic matter on the cation sorption and desorption behaviour of three imidazolium based ionic liquids -1-butyl-3-methyl-imidazolium tetrafluoroborate (IM14 BF(4)), 1-methyl-3-octyl-imidazolium tetrafluoroborate (IM18 BF(4)) and 1-butyl-3-methyl-imidazolium bis[(trifluoromethyl)sulfonyl]imide (IM14 (CF(3)SO(2))(2)N) - in soil. The German standard soil Lufa 2.2 - a natural soil classified as a loamy sand - was the basis substrate for the different soil compositions and also served as a reference soil. The addition of organic matter and clays increases the sorption of the substances and in particular smectite had striking effects on the sorption capacity for all three ionic liquids indicating that ionic interactions play an important role for sorption and desorption processes of ionic liquids in soil. One exception was for kaolinite-containing soils and the IM14 cation: with (CF(3)SO(2))(2)N(-) as an anion the sorption was identical at either 10 wt% or 15 wt% clay content, and with BF(4)(-) sorption was even lower at 15 wt% kaolinite than at 10 wt%. Desorption was weak for IM18 BF(4), presumably owing to the longer alkyl side chain. With regard to the influence of kaolinite on desorption, the same pattern was observed as it was found for the sorption of IM14 BF(4) and IM14 (CF(3)SO(2))(2)N. 相似文献
106.