Transport and survival of viruses in the subsurface—processes,experiments, and simulation models

The remediation of groundwater contaminated with waterborne pathogens, in particular with viruses, is based on their probable or actual ability to be transported from the source of origin to a point of withdrawal while maintaining the capacity to cause infections. The transport is often associated with both the unsaturated and saturated subsurface composed of varying geological settings with commensurate hydrogeological variability. Included among the most important hydrogeological factors that can be used to evaluate viral transport are the flux of moisture in the unsaturated zone, the media through which the particles travel, the length of the flow path, and the time of travel. With respect to the movement and inactivation of viruses in the subsurface, the vadose zone can provide an effective barrier for movement into groundwater and for the protection of downgradient points of withdrawal and use. Models developed to predicate viral transport in soil and groundwater are introduced, including screening models and more sophisticated predictive numerical models. As evidenced by the exponential growth of virus transport research in the literature, as well as a continuing interest in human health, the subject will continue to be one of critical importance to professionals active in the development, treatment, and conveyance of groundwater in the future. © 2005 Wiley Periodicals, Inc.     

In their words: How Detroit residents perceive the effects of their physical environment

This article examines results from in-depth interviews conducted with parents of children with asthma living in an urban environment. We examine themes from the in-depth interviews related to participants' perceptions of the influence of their physical environment on their mental and physical health, and the actions that they take in an effort to protect their health within this context. The findings from this study suggest several implications for understanding the interface between the physical environment of urban communities and residents' health, as well as for the policy and practice of risk assessment strategies.     

Which comes first,organizational culture or performance? A longitudinal study of causal priority with automobile dealerships

Prior research supports a link between organizational culture and performance but generally falls short of establishing causality or determining the direction of a culture–performance (C‐P) relationship. Using data collected from 95 franchise automobile dealerships over 6 years, we studied longitudinal culture–performance relationships to determine whether culture or performance has causal priority, or alternatively, whether a reciprocal relationship exists. Results from cross‐lagged panel analyses indicate that culture “comes first,” consistently predicting subsequent ratings of customer satisfaction and vehicle sales. Furthermore, the positive effect of culture on vehicle sales is fully mediated by customer satisfaction ratings. Copyright © 2015 John Wiley & Sons, Ltd.     

Population genetic variation in the New Zealand greenshell mussel: locus-dependent conflicting signals of weak structure and high gene flow balanced against pronounced structure and high self-recruitment

A pronounced north–south multitaxon genetic discontinuity occurs in central New Zealand (NZ). Polymorphic microsatellite markers have been used to test the location and structure of this discontinuity in the endemic greenshell mussel, Perna canaliculus. Nine neutral loci revealed limited evidence of genetic structure, but one outlier locus (Pcan1-27) which may be under selection provided evidence of the discontinuity in central NZ. Whilst the limited multilocus evidence of structure is assumed to result from high levels of gene flow among populations of this continuously distributed species, assignment tests indicated high to very high mean levels of self-recruitment within the 14 populations and the north and south regions. The nine neutral loci were unable to provide further clarification as to the geographic location of the discontinuity, whereas the Pcan1-27 locus was particularly informative. These results highlight a tension between limited evidence of genetic structure and presumptive high gene flow among populations versus high levels of self-recruitment and pronounced structure depending on microsatellite loci and analyses in question. Evidence from all 10 loci indicates that the genetic discontinuity is maintained by high levels of self-recruitment, and evidence from Pcan1-27 suggests that selection may also be important in explaining the existence of the discontinuity.     

Biofiltration of airstreams contaminated with MTBE

The treatment of groundwater contaminated with low concentrations of methyl tertiary butyl ether (MTBE) is of nationwide concern. Many treatment techniques include removing MTBE vapors from groundwater, resulting in airstreams that require treatment. One method used for air‐phase MTBE treatment is biofiltration. In a biofilter, the vapors pass through a reactor that contains MTBE‐biodegrading organisms attached to a porous media. This article reports the results of a biofiltration study to treat air contaminated with MTBE at concentrations of 0.2 to 0.33 mg/l, concentrations frequently encountered in the field. The results indicate that MTBE removal at these low concentrations is not as efficient as removals seen at higher concentrations. Activated carbon was shown to be a superior biofiltration medium, compared with media that do not adsorb MTBE vapors. Activated carbon was especially helpful in treatment shock loads of MTBE. © 2002 Wiley Periodicals, Inc.     

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin-EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.