Nutritional exchange in a tropical tripartite symbiosis: direct evidence for the transfer of nutrients from anemonefish to host anemone and zooxanthellae

The relationship between anemones and anemonefishes is an oft-cited and endearing example of a mutualistic symbiosis. Current research on mutualistic symbioses suggests these relationships are more commonplace and have greater importance at the ecosystem level on nutrient dynamics and evolutionary processes than previously thought. Using stable isotopes ¹⁵N and ¹³C, both field and laboratory experiments were designed to investigate whether nutrient transfer from two species of resident anemonefishes (Amphiprion perideraion and A. clarkii) to host anemones (Heteractis crispa) occurs. Mass spectroscopy indicated that both ¹⁵N and ¹³C were significantly elevated in the tissues of anemonefishes and in both host anemone and zooxanthellae fractions. These experiments provide the first direct empirical evidence of nitrogen and carbon transfer from resident anemonefishes to host anemones and endosymbiotic zooxanthellae. Such transfer of elements within this intriguing tripartite association underscores the central role that nutrient dynamics contributes to the evolutionary processes of these marine symbioses.     

A macroinvertebrate assessment of Ozark streams located in lead–zinc mining areas of the Viburnum Trend in southeastern Missouri, USA

The Viburnum Trend lead–zinc mining subdistrict is located in the southeast Missouri portion of the Ozark Plateau. In 2003 and 2004, we assessed the ecological effects of mining in several watersheds in the region. We included macroinvertebrate surveys, habitat assessments, and analysis of metals in sediment, pore water, and aquatic biota. Macroinvertebrates were sampled at 21 sites to determine aquatic life impairment status (full, partial, or nonsupport) and relative biotic condition scores. Macroinvertebrate biotic condition scores were significantly correlated with cadmium, nickel, lead, zinc, and specific conductance in 2003 (r?=??0.61 to ?0.68) and with cadmium, lead, and pore water toxic units in 2004 (r?=??0.55 to ?0.57). Reference sites were fully supporting of aquatic life and had the lowest metals concentrations and among the highest biotic condition scores in both years. Sites directly downstream from mining and related activities were partially supporting, with biotic condition scores 10% to 58% lower than reference sites. Sites located greater distances downstream from mining activities had intermediate scores and concentrations of metals. Results indicate that elevated concentrations of metals originating from mining activities were the underlying cause of aquatic life impairment in several of the streams studied. There was general concurrence among the adversely affected sites in how the various indicators responded to mining activities during the overall study.     

Biofiltration of airstreams contaminated with MTBE

The treatment of groundwater contaminated with low concentrations of methyl tertiary butyl ether (MTBE) is of nationwide concern. Many treatment techniques include removing MTBE vapors from groundwater, resulting in airstreams that require treatment. One method used for air‐phase MTBE treatment is biofiltration. In a biofilter, the vapors pass through a reactor that contains MTBE‐biodegrading organisms attached to a porous media. This article reports the results of a biofiltration study to treat air contaminated with MTBE at concentrations of 0.2 to 0.33 mg/l, concentrations frequently encountered in the field. The results indicate that MTBE removal at these low concentrations is not as efficient as removals seen at higher concentrations. Activated carbon was shown to be a superior biofiltration medium, compared with media that do not adsorb MTBE vapors. Activated carbon was especially helpful in treatment shock loads of MTBE. © 2002 Wiley Periodicals, Inc.     

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin-EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.     

You shouldn't have missed it

Summary Geography in the '90s: Teaching and learning with the AAG/NCGEGuidelines for Geographic Education was the title of a session at the August, 1985, meeting of the National Council for Geographic Education (NCGE) in Breckenridge, Colorado. Participants worked with theGuidelines to establish criteria and concepts to prepare solutions to the problems of worthwhile and effective geographic education. Leaders structured the work of participants, using a tight, six-step, problem-solving, process model. This model was selected as the best way in a three-hour session to help education decision makers absorb theGuidelines and master its detail while analyzing and evaluating theGuidelines as a blueprint for transforming the way students and teachers learn and think about geography. Judging by sustained participant interest and interaction, the effort devoted to carefully drafting the details of how best to present the material did enhance learning and stimulate thinking seriously about geographic education. This paper describes the reason for the workshop (Part I), outlines major components of the workshop in Part II, and makes explicit some of the less readily discerned but critical contextual aspects of the session in Part III.Dr Dixie Ann Pemberton is Associate Professor in Geography at the Horn Point Environmental Laboratories of the University of Maryland. She has extensive experience in program development, administration, training and public information in the areas of education, geography and natural resources management.     

In their words: How Detroit residents perceive the effects of their physical environment

Abstract

This article examines results from in-depth interviews conducted with parents of children with asthma living in an urban environment. We examine themes from the in-depth interviews related to participants' perceptions of the influence of their physical environment on their mental and physical health, and the actions that they take in an effort to protect their health within this context. The findings from this study suggest several implications for understanding the interface between the physical environment of urban communities and residents' health, as well as for the policy and practice of risk assessment strategies.     

The theory of neighbourhood decline due to pariah land uses: Regaining control of the downward cycle

Abstract

Some neighbourhood environments have become dumping grounds for locally unwanted land uses (LULUs) that middle-class Americans do not want near their homes. LULUs may combine with other pariah land uses to collectively undermine the quality of the local environment, reduce investment, government services, the proportion of middle-income people and associated businesses. As important neighbourhood attributes are lost, illegal activities, derelict structures, trash-strewn lots and the concentration of poor and unhealthy people may increase. This paper describes the theory behind the impact of pariah land uses through examples of the downward spiral experienced by Camden, New Jersey and the south Bronx, New York. It then documents the experience of one community, Elizabethport, New Jersey, in reversing that downward spiral. Success in Elizabethport came from the synergistic activities of local, state and federal governments, community groups, and not-for-profit organizations as they struggled to regain control through local environmental management, rebuilding, and reducing crime. Social capital was also strengthened by using a local community health concern—that of childhood asthma. Efforts to reverse the downward spiral of urban decay from pariah land uses should be multi-faceted, spurred by local efforts that address local concerns.