Impact of collection on bark regeneration from <Emphasis Type="Italic">Stryphnodendron rotundifolium</Emphasis> Mart. in northeastern Brazil

The growing commercial demand for products with medicinal use has caused overexploitation of several plant species worldwide. To prevent the decline of these populations, the collection of these resources should be done in a sustainable way considering the time of its replacement in natural stocks. This study was designed to identify the relationship between different intensities of extraction of bark from the trunk of Stryphnodendron rotundifolium Mart. and its regeneration speed. For this, we selected two areas of Cerrado in the Northeast of Brazil, where a monitoring experiment with duration of 24 months was performed. This experiment consisted in simulating different extractive damage to assess the regeneration of bark. In each area, we selected 20 individuals, among which four treatments with five repetitions were implemented. The data showed that in both study areas, the trees regenerated their shells faster when subjected to higher collection intensities. However, this regeneration was not related to variations in rainfall in the environment.     

Effects of cold-dark storage on growth of Cylindrotheca closterium and its sensitivity to copper

Cylindrotheca closterium cells were maintained at low temperature (4+/-1 degrees C) and dark conditions up to 21 weeks to assess the effect on survival and physiological status. From a control culture under standard conditions, three densities were prepared: (A) 2 x 10(4), (B) 10 x 10(4), and (C) 25 x 10(4) cells ml(-1). Weekly, inoculums of each stored density were exposed to continuous light and at 20+/-1 degrees C. Sensitivity to copper for microalgal cultures was evaluated in order to assess possible changes in cells sensitivity due to storage. Concurrently, assays with a control culture were carried out in order to assess the sensitivity of C. closterium to copper and to be able to generate a standard sensitivity control chart with a mean value of EC50-72 h+/-2SD (standard deviation). Density-C presented higher cell yield values, between 40% and 80% relative to control culture. Cell density showed to be important feature that may be taken into account in cell storage experiments. There was an increase in sensitivity of cells submitted to storage; however results always kept in the range established as standard sensitivity with no statistically significant difference with regards to control culture. EC50-72 h mean value for the control culture was 29+/-10 mug Cul(-1), while for densities-A, B and C were 22+/-7; 23+/-9 and 23+/-8 microg Cul(-1), respectively. In spite of drastic changes in the environmental conditions due to storage, it is concluded that C. closterium cells stored during 5 months remained metabolically active and with no significant change in its sensitivity.     

Spatial variation of DDT and its metabolites in fish and sediment from Cinca River, a tributary of Ebro River (Spain)

The Cinca River is a tributary of Ebro River in the NE of Spain which receives input from different activities. One of the most important is related to an industry where DDT is used as an intermediate in the production of dicofol. This study evaluated the DDT contamination along the Cinca River. Sampling sites were selected up- and downstream from this industry. Sediments and fishes (59 bleaks (Alburnus alburnus) and 23 barbels (Barbus graellsi)) were collected in 2002 and analyzed using a new and rapid selective pressurized liquid extraction (SPLE) method. DDT and its metabolites were found in sediments and fishes at levels ranging from 9 to 94microg kg(-1) dry weight (d.w.) and from not detected to 2098microg kg(-1) wet weight (w.w.), respectively. The highest values corresponded to samples collected just downstream the industry. Thirty kilometers downstream from the factory, levels were clearly lower, showing a weakening of the impact. p,p'-DDE isomer comprised up to 50% and 70% of total DDT measured in sediment and fish, respectively. When compared with values obtained in a previous study in 1999, a generalized drop of the levels in all matrixes (77-97%), was observed. No meaningful differences were found between the two fish species studied neither between the two tissues (muscle and liver) analyzed.     

A Quantitative Climate-Match Score for Risk-Assessment Screening of Reptile and Amphibian Introductions

Assessing climatic suitability provides a good preliminary estimate of the invasive potential of a species to inform risk assessment. We examined two approaches for bioclimatic modeling for 67 reptile and amphibian species introduced to California and Florida. First, we modeled the worldwide distribution of the biomes found in the introduced range to highlight similar areas worldwide from which invaders might arise. Second, we modeled potentially suitable environments for species based on climatic factors in their native ranges, using three sources of distribution data. Performance of the three datasets and both approaches were compared for each species. Climate match was positively correlated with species establishment success (maximum predicted suitability in the introduced range was more strongly correlated with establishment success than mean suitability). Data assembled from the Global Amphibian Assessment through NatureServe provided the most accurate models for amphibians, while ecoregion data compiled by the World Wide Fund for Nature yielded models which described reptile climatic suitability better than available point-locality data. We present three methods of assigning a climate-match score for use in risk assessment using both the mean and maximum climatic suitabilities. Managers may choose to use different methods depending on the stringency of the assessment and the available data, facilitating higher resolution and accuracy for herpetofaunal risk assessment. Climate-matching has inherent limitations and other factors pertaining to ecological interactions and life-history traits must also be considered for thorough risk assessment. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Hydroxycinnamic acid derivatives in an aquatic liverwort as possible bioindicators of enhanced UV radiation

We examined, under laboratory conditions, the physiological responses of the aquatic liverwort Jungermannia exsertifolia subsp. cordifolia to artificially enhanced ultraviolet (UV) radiation for 82 days, especially considering the responses of five hydroxycinnamic acid derivatives. This species lives in mountain streams, where it is exposed to low temperatures and high UV levels, and this combination is believed to increase the adverse effects of UV. Enhanced UV radiation hardly caused any change in several physiological variables indicative of vitality, such as Fv/Fm and chlorophylls/phaeopigments ratio (OD430/OD410). Thus, this liverwort seemed to be tolerant to UV radiation, probably due to the accumulation of three UV-absorbing hydroxycinnamic acid derivatives: p-coumaroylmalic acid, 5'-(7',8'-dihydroxycoumaroyl)-2-caffeoylmalic acid, and 5'-(7',8'-dihydroxy-7-O-beta-glucosyl-coumaroyl)-2-caffeoylmalic acid. These compounds might serve as bioindicators of enhanced UV radiation.     

Multimethod,multistate Bayesian hierarchical modeling approach for use in regional monitoring of wolves

In many cases, the first step in large‐carnivore management is to obtain objective, reliable, and cost‐effective estimates of population parameters through procedures that are reproducible over time. However, monitoring predators over large areas is difficult, and the data have a high level of uncertainty. We devised a practical multimethod and multistate modeling approach based on Bayesian hierarchical‐site‐occupancy models that combined multiple survey methods to estimate different population states for use in monitoring large predators at a regional scale. We used wolves (Canis lupus) as our model species and generated reliable estimates of the number of sites with wolf reproduction (presence of pups). We used 2 wolf data sets from Spain (Western Galicia in 2013 and Asturias in 2004) to test the approach. Based on howling surveys, the naïve estimation (i.e., estimate based only on observations) of the number of sites with reproduction was 9 and 25 sites in Western Galicia and Asturias, respectively. Our model showed 33.4 (SD 9.6) and 34.4 (3.9) sites with wolf reproduction, respectively. The number of occupied sites with wolf reproduction was 0.67 (SD 0.19) and 0.76 (0.11), respectively. This approach can be used to design more cost‐effective monitoring programs (i.e., to define the sampling effort needed per site). Our approach should inspire well‐coordinated surveys across multiple administrative borders and populations and lead to improved decision making for management of large carnivores on a landscape level. The use of this Bayesian framework provides a simple way to visualize the degree of uncertainty around population‐parameter estimates and thus provides managers and stakeholders an intuitive approach to interpreting monitoring results. Our approach can be widely applied to large spatial scales in wildlife monitoring where detection probabilities differ between population states and where several methods are being used to estimate different population parameters.     

Bioassays for the evaluation of reclaimed opencast coal mining areas

Environmental Science and Pollution Research - This study aimed to use bioassays (single and multispecies) with organisms from different trophic levels to assess soil quality in reclaimed coal...     

Soil carbon stock estimations: methods and a case study of the Maranhão State,Brazil

Environment, Development and Sustainability - Soils feature significant variations in soil carbon stocks through land-use changes, management practices, and intrinsic characteristics. The aim of...     

Impact of roof rain water harvesting of runoff capture and household consumption

Environmental Science and Pollution Research - In recent years, the occurrence of floods is one of the most important challenges facing in Hamadan city. In the absence/inefficiency of urban...