Monitoring PCDD/Fs, PCBs and metals in the ambient air of an industrial area of Catalonia, Spain

In 2005 and 2006, the levels of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs) and metals (As, Be, Cd, Co, Cr, Cu, Mn, Ni, Pb, Sn, Tl and V) were measured in air samples collected in an industrial area of Sant Adrià del Besòs (Barcelona, Catalonia, Spain) where a municipal solid waste incinerator (MSWI) is placed, and in a background/control area. In general terms, concentrations of all environmental pollutants were higher at the industrial site. No significant seasonal/temporal variations were observed in any of the areas. No Pearson correlation was found between the PCDD/F concentrations and the environmental conditions of the two sampling periods considered. Principal component analyses (PCA) were performed to get information on the relationship among samples, pollutants, and emission sources. The results indicate that the MSWI of S. Adrià de Besòs is not a significant emission source of the above compounds for the area under its direct influence. Moreover, a notable difference in the PCDD/F congener profiles was found between ambient air and stack gas emissions, indicating that the current levels of PCDD/Fs are more related to other potential emissions sources rather than to those from the MSWI.     

Comparison of surface emissions and subsurface distribution of cis- and trans-1,3-dichloropropene and chloropicrin in sandy field beds covered with four different plastic films

The purpose of this study was to conduct a field study at a Florida field site on surface emissions and subsurface distribution of cis-and trans-1,3-dichloropropene (1,3-D) and chloropicrin (CP) in raised beds injected with Telone C35 with four replications. A total of 16 beds were applied with Telone C35 by chisel injection and covered with four different plastic films, 4 beds for each film. Each bed was installed with five 20-cm long soil pore air probes and a surface air collection pan at arbitrarily locations along the length of each bed for sampling soil pore air and surface air, respectively, for analysis of the three biologically active compounds, cis- and trans-1,3-D and CP. We found that average concentrations of the three compounds at 20-cm depth among the beds covered with four different plastic films generally were not statistically different. Among the four beds covered with the same plastic film, average concentrations of the three compounds were statistically different only in the four metallic PE covered beds at 5 and 24 hours after injection. Volatilization rates of the three compounds among the beds covered with four different plastic films, with the exception of CP at 48 hours after injection, were not statistically different. It appeared that initial upward diffusion and volatilization flux were influenced by solar radiation. Initial subsurface concentrations of the three compounds and volatilization flux, especially cis-1,3-D, were greater in the beds on the east side of the field than that in the beds on the west side of the field. Whether or not difference in initial subsurface concentrations of the compounds between east side beds and west side beds may influence fumigant efficacy remains to be determined.     

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogenproducing bacteria, such as Clostridium, and promoted the dominance of acetoclastic- over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.     

Genotoxicity biomarkers in the amphipod Gammarus elvirae exposed in vivo to mercury and lead,and basal levels of DNA damage in two cell types

The present work deals with the application of genotoxicity biomarkers by means of the Comet assay in haemocytes and spermatozoa of the crustacean Gammarus elvirae exposed in vivo to heavy metals. Furthermore, a basal levels (BLs) study of DNA damage in the two cell types considered for two different gammarids species, G. elvirae and Echinogammarus veneris, was carried out. It is important to identify factors that influence the outcome of the assay in order to obtain reliable and reproducible results usable for risk assessment purposes. Our results highlight that the Italian legal limits for Hg and Pb, respectively, 0.5 and 50?µg/L, are inadequate for establishing safety thresholds in the aquatic environment. Furthermore, the freshwater invertebrate G. elvirae, used for the first time to measure the effect of genotoxicants, is a good candidate for evaluating the genotoxicity damage induced by heavy metals. Our results concerning spermatozoa show excessively variable responses and high BLs.     

Comparative analysis of two tobacco surveillance questionnaires used in NHANES: accuracy of self-reported smoking status

National Health and Nutrition Examination Survey (NHANES) uses two tobacco use surveillance questionnaires. One is administered during an at-home interview (HI) and the other, during an examination session at a mobile examination center (MEC). NHANES data for the years 1999–2012 were used to evaluate the consistency of responses to tobacco surveillance questions in HI and MEC interview. In addition, accuracy of self-reported smoking status was evaluated. Of those who reported to be daily cigarette smokers during HI, 18.7% reported to be either some-day smokers or nonsmokers during MEC interview. Of those who reported to be some-day cigarette smokers during HI, 22.1% reported to be daily smokers and 17.5% reported to be nonsmokers. Also, 4.1% of those who reported to be nonsmokers during HI reported to be either daily or some-day smokers during MEC interview. Using serum cotinine measurements taken during MEC interview, 27.1% were found to be smokers and 72.9% were found to be nonsmokers. In general, a moderate to high agreement, as measured by the κ statistic, was found between the self-reported responses to tobacco use questions during the home and MEC interviews as well as between smoking statuses based on self-reported and serum cotinine measurements.     

Prediction of mass and momentum transport in turbulent plane wall jets over smooth and transitionally rough surfaces

This paper is concerned with the prediction of mass and momentum transport in turbulent wall jets developing over smooth and transitionally rough plane walls. The ability to accurately predict the resulting wall shear stresses and vertical profiles of the Reynolds stresses in these flows is prerequisite to the accurate prediction of bed scour, sediment re-suspension and transport by turbulent diffusion. The computations were performed by solving the Reynolds-averaged forms of the equations describing conservation of mass, momentum and concentration. The unknown correlations that arise from the averaging process (the Reynolds stresses in the case of the momentum equation, and the turbulent mass fluxes in the case of concentration) were obtained from the solution of modeled differential equations that describe their conservation. Since these models are somewhat more complex than those typically used in practice, their benefits are demonstrated by comparisons with results obtained from simpler, eddy-viscosity based closures. Comparisons with experimental data show that results of acceptable accuracy can be obtained only by using the appropriate combination of models for the turbulent fluxes of mass and momentum that properly account for the reduction of the Reynolds stresses due to wall damping effects, and for the modification of the mass transfer rates due to interactions with the mean rates of strain.