STREAM CHEMISTRY IN THE SOUTHERN BLUE RIDGE: FEASIBILITY OF A REGIONAL SYNOPTIC SAMPLING APPROACH1

ABSTRACT: A pilot study, which was conducted in the Southern Blue Ridge geographical province of the Southeastern U.S., demonstrated the feasibility of a probability-based regional synoptic design for the National Stream Survey, which is a project aimed at estimating the number and percentage of streams in various regions of the U.S. that are acidic or at risk from acid deposition. Estimated population distributions for key chemical variables were not appreciably affected by week-to-week variability in stream chemistry during the spring index period chosen for the study. Differences were observed in estimated acid neutralizing capacity (ANC), nitrate, and pH frequency distributions between spring and summer. Observations made at the downstream node did not represent the chemistry of the entire reach for some variables (ANC and nitrate) as indicated by differences in chemical concentrations between upstream and downstream sampling locations. Coefficients of variation in chemical species were low enough to provide a reasonably stable classification of streams based on ANC. Although median ANC, sulfate, and nitrate concentrations were quite low in the region, the probability of finding streams with ph < 6.3 is less than 1.3 percent at the α= 0.05 confidence level.     

The response of female Mountain White-crowned Sparrows to songs from their natal dialect and an alien dialect

Summary Female Mountain White-crowned Sparrows from Colorado were tested in a laboratory playback experiment to determine their response to male songs from their own natal dialect and to those from an alien dialect of the same subspecies, Zonotrichia leucophrys oriantha (Fig. 1). Observations were made on locomotor activity and copulation postures produced during each experimental test session.The subjects gave copulation displays almost exclusively when hearing their natal dialect and new or no displays when hearing the alien dialect (Fig. 2). The birds also exhibited significantly greater locomotor activity when hearing the natal dialect than they did when hearing the alien dialect (Fig. 3).We conclude that females are sensitized by their early song learning experience to be responsive to songs from their natal dialect and virtually sexually unresponsive to songs from an alien dialect. We speculate that the copulation posture given in the experiment implies that females in natural populations would preferably only mate assortatively with males from their natal dialect region.     

Behaviour of the institute of oceanographic science's rectangular midwater trawls: Theoretical aspects and experimental observations

The behaviour of two rectangular midwater trawls, the RMT 1+8 and the RMT 1+8M, is described. The RMT 1+8 consists of a pair of rectangular nets contained within the same frame-a small mouth area RMT 1 of mesh size 320 m and a larger RMT 8 of mesh size 4.5 mm. The RMT 1+8M is a multiple version of the RMT 1+8, combining three RMT 1s and three RMT 8s within the same frame. An expression is developed to describe the dependence of mouth angle on speed for the RMT 1+8 when towed horizontally. Theoretical estimates of the changes in mouth angle with speed agree well with experimental observations. Within normal towing speeds the RMT 1+8 is sensitive to changes in speed; this sensitivity is especially marked in the RMT 1. Between speeds of about 2.6 and 8.0 knots the RMT 8 does not alter its mouth angle with changes in speed; this behaviour is not shown by the RMT 1—at least within a practical speed range. The performance of the RMT 1+8M cannot be completely described by the theory developed for the RMT 1+8 and possible explanations for this are discussed. The multiple net is considerably less sensitive to changes in towing speed; all three nets of each type have similar mouth angles at a constant speed. Variations in warp load with speed for both trawls are described.     

The role of herbicides in the erosion of salt marshes in eastern England

Laboratory studies and field trials were conducted to investigate the role of herbicides on saltmarsh vegetation, and their possible significance to saltmarsh erosion. Herbicide concentrations within the ranges present in the aquatic environment were found to reduce the photosynthetic efficiency and growth of both epipelic diatoms and higher saltmarsh plants in the laboratory and in situ. The addition of sublethal concentrations of herbicides resulted in decreased growth rates and photosynthetic efficiency of diatoms and photosynthetic efficiency of higher plants. Sediment stability also decreased due to a reduction in diatom EPS production. There was qualitative evidence that diatoms migrated deeper into the sediment when the surface was exposed to simazine, reducing surface sediment stability by the absence of a cohesive biofilm. Sediment loads on leaves severely reduced photosynthesis in Limonium vulgare. This, coupled with reduced carbon assimilation from the effects of herbicides, could have large negative consequences for plant productivity and over winter survival of saltmarsh plants. The data support the hypothesis that sublethal herbicide concentrations could be playing a role in the increased erosion of salt marshes that has occurred over the past 40 years.     

Determination and comparison of nitrated-polycyclic aromatic hydrocarbons measured in air and diesel particulate reference materials

The National Institute of Standards and Technology has issued numerous environmental matrix standard reference materials (SRMs) for the measurement of polycyclic aromatic hydrocarbons; however, only one SRM (diesel particulate material) was issued with certified and reference values for four nitrated-polycyclic aromatic hydrocarbons (nitro-PAH). The objective of this study was to develop an improved analytical procedure to quantify 28 mononitro- and dinitro-PAHs, including numerous isomers, in air and diesel particulate SRMs. Two air particulate matrix SRMs, SRM 1649a Urban Dust and SRM 1648 Urban Particulate Matter, and fine particulate matter, collected from Baltimore MD for use as an "interim reference material" for the determination of organic contaminants, have been characterized for nitro-PAHs. Concentrations of nitro-PAHs in all three air particulate materials were at the ng/g level with the highest nitro-PAH concentration being 2-nitrofluoranthene (range between 246 and 340 ng/g). For the three diesel particulate-related SRMs, SRM 1650a Diesel Particulate Matter, SRM 1975 Diesel Particulate Extract, and SRM 2975 Diesel Particulate Matter (Industrial Fortlift), concentrations of nitro-PAHs were in the microg/g range, with 1-nitropyrene as the dominant nitro-PAH (range between 18 and 40 microg/g). Distinct nitro-PAH isomer patterns were present between the air and diesel particulate materials. These results will provide isomer identification and reference concentrations for a large number of nitro-PAHs in the existing diesel and air particulate SRMs. Published by Elsevier Science Ltd.     

Isolation of MFO inducers from tissues of white suckers caged in bleached kraft mill effluent

White sucker (Catostomus commersoni) caged for 3 days in a bleached kraft mill effluent (BKME) stream had elevated mixed function oxygenase (MFO) activities 15-90 fold those of fish caged in a reference stream. Liver composites of male and female fish were ground and extracted with dichloromethane (DCM), methanol or 50% DCM/methanol, and tested for MFO activity in rat hepatoma cells (H4IIE). There was no difference in the potency of H4IIE EROD induction among the three solvents, so DCM extracts were split into 31 fractions using reverse phase high pressure liquid chromatography (HPLC). H4IIE MFO activity was elevated in several fractions, with three early peaks and several later peaks of induction, indicating several classes or compounds causing MFO induction were present in the fish livers. Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and chlorinated diphenylethers (CDPEs) were detected in several late-eluting fractions, but concentrations were not high enough for these compounds to be solely responsible for the observed induction. Induction by liver extracts decreased as cell exposure times increased (24, 48 or 72 h), suggesting that some inducers were more easily metabolized and eliminated from the H4IIE cells. In contrast, 2,3,7,8-tetrachlorodibenzo-p-dioxin (2378-TCDD) had similar potency over 24, 48 and 72 h, as it was relatively resistant to metabolism.     

False positive fecal coliform in biosolid samples assayed using A-1 medium.

Two most probable number (MPN) methods-lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)--were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.     

Soil solution Zn and pH dynamics in non-rhizosphere soil and in the rhizosphere of Thlaspi caerulescens grown in a Zn/Cd-contaminated soil

Temporal changes in soil solution properties and metal speciation were studied in non-rhizosphere soil and in the rhizosphere of the hyperaccumulator Thlaspi caerulescens J. & C. Presl (population from Prayon, Belgium) grown in a Zn- and Cd-contaminated soil. This paper focuses on soil solution Zn and pH dynamics during phytoextraction. The concentration of Zn in both non-rhizosphere and rhizosphere soil solutions decreased from 23 mg/l at the beginning to 2 mg/l at the end of the experiment (84 days after transplanting of seedlings), mainly due to chemical sorption. There was no significant difference in overall Zn concentration between the planted and the unplanted soil solutions (P > 0.05). Soil solution pH decreased initially and then increased slightly in both planted and unplanted soil zones. From 60 to 84 days after transplanting, the pH of the rhizosphere soil solution was higher than that of non-rhizosphere soil solution (P<0.05). Zn uptake by the hyperaccumulator plants was 8.8 mg per pot (each containing 1 kg oven-dry soil) on average. The data indicate that the potential of T. caerulescens to remove Zn from contaminated soil may not be related to acidification of the rhizosphere.