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921.
M. Böhm U. Hentschel A. Friedrich L. Fieseler R. Steffen V. Gamulin I. Müller W. Müller 《Marine Biology》2001,139(6):1037-1045
The aim of this study was the documentation of the molecular immune response of Suberites domuncula upon bacterial infection. Additionally, the bacteria that are naturally present in the sponge after prolonged aquarium maintenance were characterized. After 6 months of maintenance of S. domuncula in seawater aquaria, only one bacterial 16S rDNA sequence could be recovered, which belongs to the genus Pseudomonas. Concomitantly, morphologically uniform bacteria were found encapsulated in bacteriocytes. These findings indicate that certain bacteria, possibly of the genus Pseudomonas, are able to persist for long periods in host bacteriocytes. Subsequent to performing a previously established infection assay with S. domuncula, a potentially pathogenic Vibrio sp. was isolated from the tissues. Furthermore, the host tissue disintegrated and asexual propagation bodies (gemmules) were formed. In order to gain insights into the molecular events occurring after bacterial infection, the stress-response kinases, p38 protein kinase and JNK protein kinase, were analyzed. It is demonstrated that these two kinases are activated (phosphorylated) upon incubation of the tissue with the bacterial endotoxin lipopolysaccharide (LPS). Moreover, LPS strongly inhibits protein synthesis. It is concluded that there are many functionally different interactions between S. domuncula and bacteria and that the animal possesses mechanisms to differentiate between bacteria and to respond accordingly. 相似文献
922.
Effects of salinity on endogenous rhythm of the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae) 总被引:1,自引:0,他引:1
The Manila clam Ruditapes philippinarum, an intertidal bivalve, was exposed to different salinity regimes (from 31.0–31.7‰ down to 20‰, 15‰, 10‰, 5‰), and the endogenous
rhythm in its oxygen consumption was studied using an automatic intermittent-flow respirometer. When exposed to salinities
reduced from 31.5‰ to 20‰ and 15‰ under otherwise constant conditions, the clams recovered a clear endogenous circatidal rhythm
in their oxygen-consumption rate after having dampened periods of 12 h and 48 h, respectively. At salinities less than 10‰,
however, the oxygen-consumption rate was depressed greatly at the beginning of the experiment for about 36 h and then increased
to a level higher than normal, but the rhythm of oxygen consumption was not recovered. The results of this study indicate
that the Manila clam, a euryhaline organism, cannot maintain a normal metabolic activity at a salinity lower than 15‰. All
clams were dead after exposure at a salinity of 5‰ for 7 days.
Received: 28 February 2000 / Accepted: 26 August 2000 相似文献
923.
The seaweed Sargassum polyceratium Montagne inhabits a broad spectrum of subtidal and intertidal habitats. Genetic diversity and spatial genetic structure were examined within and among 12 stands using random amplified polymorphic DNA (RAPD) phenotypes. Data were analyzed using analysis of molecular variance (AMOVA) and Shannon's information measure. In both analyses, 60-75% of the variation occurred within stands and 25-40% between stands. These values are consistent with out-crossing, high-dispersal species. Significant differentiation was found among bays ca. 25 km apart (Shannon's G'st averaged 0.37 and pairwise AMOVA Kst values averaged 0.272) and among stands 150-200 m apart within bays (AMOVA Kst values averaged 0.149). Effects of shore (windward vs. leeward), depth, and bay on population structure were tested. These analyses revealed that the factor depth is confounded with shore, and that bays show significant differentiation from each other but are not completely isolated from one another. Mantel tests for differentiation-by-distance were significant along both sides of the island but stronger along the windward side. A neighbor-joining analysis of genetic distances among stands showed that the effects of currents around both tips of the island were especially important for shallow populations. For S. polyceratium, depth and bay promote population differentiation along shores, yet dispersal around the tips of the island simultaneously connects these populations to varying degrees. This study highlights the importance of investigating the relative contribution of habitat factorsin relation to island-scale population structure. 相似文献
924.
江苏数字地震台网已建设改造了 8个台站。对 8个台站记录到的背景噪声进行定量分析 ,测定背景噪声的地动速度均方根值 (RMS)、有效测量动态范围 (D有效)和噪声功率谱。结果表明 :受台基和海潮等观测环境的影响 ,大部分台站的地动速度RMS值高于 2 × 1 0 -8m/s,降低D有效 值。背景噪声功率谱峰值频率为 2 5~ 3 0Hz。这些定量分析为了解数字地震台网的实际监测水平提供了可靠依据 相似文献
925.
蒽醌染料中间体溴氨酸降解酶的特性 总被引:5,自引:0,他引:5
从污染地分离筛选出的菌株BX26对蒽醌染料中间体溴氨酸有显著的降解脱色作用,降解过程受降解酶的控制,试验结果表明,降解酶为溴氨酸诱导的胞外酶,该酶在温度高于50℃处理后失活,盐度对该酶失活有影响,盐度高于1%会显著降低该酶活力,酶对溴氨酸的催化脱色要有氧参加,氮气气氛中酶活受抑制。 相似文献
926.
天津市中心城区空气污染物时间变化规律的研究 总被引:5,自引:0,他引:5
根据空气质量标准,分析了SO2、NO2和PM103项污染物不同季节,不同时期,不同时段变化规律、达标状况以及为制定相应的防治措施提供参考依据。 相似文献
927.
The focus of this paper is on the dynamics of a dense current flowing along the continental slope, and the frictionally induced downward motion it experiences. In particular, the movement of the lateral boundaries where the isopycnals meet the bottom are considered. The current is taken to be wide compared to the Rossby radius, which is in accordance with observations and makes the dynamics quasigeostrophic. The time development of the plume thickness is studied, using three different parameterisations for the bottom friction.Independently of the choice of parameterisation, the following results are obtained: In the central part of the plume friction acts as a diffusive process to minimise the curvature of the upper surface of the plume. At the upper edge the plume quickly approaches a state with small slope, i.e. small geostrophic velocity and small frictionally induced downward flow. At the lower edge a tongue of dense water shoots out creating a widening layer. The thickness of the migrating layer is approximately equal to the boundary layer depth and its downward speed is comparable to the along-slope geostrophic velocity. The downslope end of the migrating layer may form a steep front which requires some precautions in the numerical procedure. 相似文献
928.
Green sea urchins, Strongylocentrotus droebachiensis OF Müller, collected off the coast of New Hampshire, USA, in late February 1995, were brought into the laboratory and fed
an artificial diet ad libitum, and subjected to a photoperiod advanced by 4 months. During this study, temperatures and salinities
for experimental urchins mirrored those recorded at the collection site. We examined the effects of changes in feeding regime
and photoperiod on gametogenesis and compared the experimental urchins with those from the source population. During the 7-month
period, experimental urchins showed no detectable changes in mean test height or diameter. Experimental urchins had a significantly
higher gonad index (GI) in March, April and May (18 ± 6%) compared with field urchins in March (11 ± 3%). Subsequently, experimental
urchins had a mean monthly GI of 25 to 30%, while the mean GI for field urchins was 11 to 13%. Gonial cell mitosis and gametogenesis
occurred earlier in experimental male and female urchins compared with field urchins. Stereological and histological observations
and stage–frequency data showed that the ovaries of experimental urchins were large because of the accelerated development
of nutritive phagocytes, the volume fraction (V
v) of which was 89 to 90% of the gonad, while new vitellogenic primary oocytes occupied <1% V
v. In males, stereological and histological observations and stage–frequency data suggested a mobilization of materials from
the nutritive phagocytes beginning between June and August, i.e. earlier than in females, and, by September, new gametes occupied
a V
v of 49 ± 3% of the testes. Oocyte size–frequency distributions demonstrated that most primary oocytes were <80 μm in diameter
between March and September, suggesting that cold temperatures may be needed for completion of vitellogenesis. We describe
changes in the two principal cell types in the germinal epithelium of urchin gonads and indicate how knowledge of their population
dynamics may be useful in aquaculture applications.
Received: 21 February 1997 / Accepted: 3 June 1998 相似文献
929.
The predation impact of the two chaetognaths Eukrohnia hamata and Sagitta gazellae on mesozooplankton standing stock were investigated in three depth layers during two 24 h stations occupied in the vicinity
of Marion Island in late austral summer (April/May) 1986. The zooplankton community at both stations was dominated by small
copepods (Oithona spp., Microcalanus spp.), which accounted for >95% of total zooplankton abundance. Chaetognaths comprised <2% of total zooplankton abundance.
E. hamata constituted >95% of the total chaetognath stock. The general trend in both species was decreasing abundance with increasing
depth, which appeared to be correlated to the distribution of copepods (r
2 = 0.45; P <0.05). Gut-content analysis showed that copepods (mainly Oithona spp., Calanus spp. and Rhincalanus gigas) and ostracods were the main prey of both species, accounting for 87 and 61% of the total number of prey in E. hamata and S.␣gazellae stomachs, respectively. In the guts of S.␣gazellae, pteropods (Limacina spp.) and chaetognaths were also well represented. The mean number of prey items (NPC) for E. hamata ranged from 0.02 to 0.06 prey individual−1 which corresponds to an individual feeding rate (Fr) of between 0.05 and 0.12 prey d−1. For S.␣gazellae, the NPC values were higher, varying between 0.04␣and 0.20 prey individual−1, or between 0.15 and 0.76 prey d−1. The daily predation impact of the two chaetognaths was estimated at between 0.3 and 1.2% of the copepod standing stock or
between 7 and 16% of the daily copepod production. Predation by S. gazellae on chaetognaths accounted for up to 1.6% of the chaetognath standing stock per day.
Received: 26 November 1996 / Accepted: 31 October 1997 相似文献
930.
Common shrimp, Crangon crangon (L.), were exposed to inorganic arsenic (arsenate), trimethylarsine oxide, or arsenobetaine in sea water (100 μg As l−1) or in food (1 mg As g−1 wet wt) for up to 24 d, followed by 16 d depuration in clean sea water with undosed food, in order to determine the efficiency
of uptake and retention of the compounds. Accumulation of arsenic in the tail muscle, gills, midgut gland, exoskeleton, and
remaining tissues was found to depend on the chemical form of the arsenic and the route of exposure. No arsenic was accumulated
by C. crangon exposed to arsenate or trimethylarsine oxide in sea water. Shrimps exposed to waterborne arsenobetaine initially accumulated
a small amount of arsenic in their tail muscle and gills. After 16 d, C. crangon fed arsenate, trimethylarsine oxide, or arsenobetaine had accumulated arsenic in their tail muscle to levels ∼2-, 2-, or
40-times, respectively, that of the control group. A roughly linear rate of accumulation was shown by shrimps fed trimethylarsine
oxide or arsenobetaine, but C. crangon fed arsenate accumulated arsenic for 16 d, then lost arsenic such that their concentration on Day 24 was not significantly
different from that of the control group. Patterns of arsenic accumulation in the gills of shrimps fed the compounds were
similar to those seen in the tail muscle. On a whole animal basis, C. crangon retained ∼1.2% of the arsenate, 1.6% of the trimethylarsine oxide, and 42% of the arsenobetaine consumed over the first 16 d
of exposure, with roughly half present in the tail muscle in each case. Data obtained support the view that the direct uptake
of arsenobetaine from sea water does not make a significant contribution to the relatively high concentrations of this compound
in marine crustaceans, and that food is the primary source. Naturally occurring arsenic compounds in C. crangon and possible transformations of the administered arsenic compounds were examined by high performance liquid chromatography
using an inductively coupled plasma mass spectrometer as the arsenic-specific detector. Control C. crangon contained arsenobetaine as the major arsenic compound (>95% of total arsenic); tetramethylarsonium ion (0.7%) and an unknown
arsenic compound (1.7%) were also present as minor constituents. Shrimp ingesting arsenobetaine accumulated it unchanged.
Shrimp ingesting arsenate did not form methylated arsenic compounds; they appeared to contain their accumulated arsenic as
unchanged arsenate only, although the possibility that some of the arsenic was reduced to arsenite could not be excluded.
C. crangon ingesting trimethylarsine oxide biotransformed the compound predominantly to dimethylarsinate.
Received: 9 October 1997 / Accepted: 11 February 1998 相似文献