Biodegradability of soil water soluble organic carbon extracted from seven di erent soils

Water soluble organic carbon (WSOC) is considered the most mobile and reactive soil carbon source and its characterization is an important issue for soil ecology study. A biodegradability test was set up to study WSOC extracted from 7 soils di erently managed. WSOC was extracted from soil with water (soil/water ratio of 1:2, W/V) for 30 min, and then tested for biodegradability by a liquid state respirometric test. Result obtained confirmed the finding that WSOC biodegradability depended on the both land use and management practice. These results suggested the biodegradability test as suitable method to characterize WSOC, and provided useful information to soil fertility.     

Precarious work or sustainable livelihoods? Aligning Prestea's Programme with the development dialogue on artisanal and small‐scale mining

Alternative livelihoods programmes (ALPs) are extensively executed in mining communities, often as models of development dialogue on artisanal and small‐scale mining (ASM). This paper assesses whether Prestea's ALP aligns with the development dialogue on artisanal mining. The conceptual design of ALP in Ghana's Prestea is based on the notions of substitution, homogenous community, and impact scalability. This paper argues that the Prestea ALP is not aligned with the development dialogue on artisanal mining, and therefore it is difficult to understand the role and function of environmentally‐damaging behaviours within livelihood strategies. The paper contends that it would be appropriate to concentrate on improving the existing artisanal miners’ operation of those most susceptible to resource access restrictions. Further, it may be more prudent to utilize livelihood‐centered interventions that create strong connections with sustainable development as a way of creating regular community engagements. Additionally, this paper argues that the term for the intervention programme on artisanal mining should be replaced with the broader term ‘livelihood‐centered intervention’. The replacement of the term ‘ALP’ avoids the tacit belief that ALP can adequately replace artisanal mining operations. Livelihood‐centered intervention should not necessarily utilize alternative livelihoods as direct behavioural change instruments.     

Amphibian myiasis. Blowfly larvae (Lucilia bufonivora,Diptera: Calliphoridae) coping with the poisonous skin secretion of the common toad (Bufo bufo)

The blowfly Lucilia bufonivora shows high host specificity for toads despite the host’s toxic skin secretion, which consists mainly of bufadienolides. These toxins are effective blockers of the Na⁺, K⁺-ATPase, an enzyme that is essential for many physiological processes in animals. Whereas common toad (Bufo bufo) toxins were identified in the larvae of the fly, few toxins were found in the pupae and empty puparia as trace amounts, while adult flies were entirely free of these toxic compounds. Similar results were obtained when larvae of generalist necrophagous blowflies (L. sericata, Calliphora vicina) fed on toad carcasses. Analysis of the Na⁺, K⁺-ATPase gene revealed no amino acid substitution at positions known to mediate resistance to bufadienolides in other systems. Alternative mechanisms of resistance such as efficient excretion of the compounds may enable the flies to use this poisonous food source.     

Model reactions of chromium compounds with mammalian and bacterial cells†

Chromate uptake, reduction, cytotoxicity and mutagenicity were studied with human red blood cells, Chinese hamster ovary (CHO) cells and/or Salmonella typhimurium mutant cells. All cell types rapidly took up chromates whereas chromium(III) salts were excluded under the experimental conditions. Red blood cells reduced and accumulated chromium from chromate. At concentrations above 0.1 mM, chromate inactivated the red cell chromate carrier. Chromate above 0.01 mM inhibited CHO cell proliferation irrespective of the cations present. Chromate and two chromium(III) complexes were mutagenic with Salmonella mutants in the Ames’ assay. A model for chromate metabolism and genotoxicity is proposed.     

The cytotoxicity of nici2 for mammalian cells in culture†

The effects of NiCl₂ were studied in two human cell lines, HeLa and diploid embryonic fibroblasts as well as in V79 Chinese hamster cells and in L‐A mouse fibroblasts. NiCl₂ produces a dose‐dependent depression of proliferation, mitotic rate, and viability, accompanied by an increasing release of lactic dehydrogenase and stimulation of lactic acid production. The plating efficiency is reduced, as are DNA and protein synthesis and, to a lesser degree, RNA synthesis.

The cytotoxicity of NiCl₂ is comparable in degree to those of PbCl₂ and MnCl₂, but is weaker than those of HgCl₂ and CdCl₂. However, the different sensitivities of different cell lines must also be considered.

NiCl₂ effects are more severe in serum‐free medium than in medium containing serum or serum albumin indicating that serum constituents, notably albumin, bind the metal effectively and inhibit cellular uptake; this confirms earlier reports on the serum binding and slow uptake of NiCl₂.

Synchronized cells are most sensitive in the Gl and early S phases of the cell cycle. In the Painter test the depression of DNA synthesis persists following cessation of exposure to NiCI₂. These findings contribute an explanation for the known genotoxic effects of nickel.     

How age and sex drive the foraging behaviour in the king penguin

As predicted by life history theory, once recruited into the breeding population and with increasing age, long-lived animals should be able to manage more efficiently the conflict between self-maintenance and reproduction. Consequently, breeding performances should improve with age before stabilizing at a certain level. Using temperature–depth recorders and isotopic analysis, we tested how age affects the foraging behaviour of king penguin Aptenodytes patagonicus during one trip in the chick-rearing phase. Depending on sex and age, king penguins expressed two different foraging strategies. Older birds gained more daily mass per unit body mass than younger ones. Older females conducted shorter trips, dived deeper and performed more prey pursuits. They also had higher blood levels of δ¹⁵N than younger individuals and males indicating sex- and age-specific dietary regimes. However, we found no differences in carbon isotopic signature, suggesting that individuals exploited the same foraging areas independently of sex and age. Our results suggest that king penguins are able to increase the quantity of energy extracted with increasing age and that such a strategy is sex-related. Our study is the first to reveal of an interaction between age and sex in determining foraging efficiency in king penguins.     

An ecosystem model for estimating potential shellfish culture production in sheltered coastal waters

A generic ecosystem model has been developed for estimating the potential production of shellfish culture and the effect of that cultivation on the pelagic ecosystem in sheltered coastal waters. The model describes the dynamics of a simple food web, nutrient cycling and growth of shellfish. The design of the model is closely tied to the temporal and spatial scales that are important in determining the sustainable production level for a particular embayment. The pelagic ecosystem, mussel energetics, population dynamics and hydrodynamics are coupled to allow fully dynamic predictions of the effect of the shellfish density. When applied to Beatrix Bay, an intensive culture embayment in the Pelorus Sound of New Zealand, the model successfully captured main features of the observed system behaviour. The hydrodynamic regime of the bay controls mussel growth and production. Although high fluxes of water into the bay suppress nutrient and carbon cycling signals in the system, the model simulations demonstrated that the mussel cultivation can have considerable effects on the ecosystem of the bay including food depletion and nutrient cycling. One of the most obvious effects is nutrient enhancement through mussel excretion at low cultivation densities, which promotes primary production particularly during the N-limitation period in summer. The sensitivity analysis identified uncertainty in some parameters and indicated areas for which experimental studies could lead to model improvement. The modelling exercise has established a primary predictive tool for managing mussel aquaculture of a coastal embayment to estimate relationships between the stock level and the growth rate of mussels, and the potentially achievable harvest and stocking density.     

Monitoring of Bt-Maize pollen exposure in the vicinity of the nature reserve Ruhlsdorfer Bruch in northeast Germany 2007 to 2008

Background, aim, and scope Basically, technological innovations are associated with benefits and risks. This is also true for the introduction of genetically modified organisms (GMO) into agriculture. In Germany, precautionary regulations currently demand isolation distances (i.?e. buffer zones) for the cultivation of genetically modified maize (Bt-maize) in the vicinity of conventional (150?m) and organic maize fields (300?m). The Bt-toxin may harm non-target organisms (NTO) such as Lepidoptera. Despite this, corresponding regulations for the protection of nature reserves are lacking to date. Conventional and Bt-maize have been grown in the vicinity of the Flora-Fauna-Habitat (FFH) Ruhlsdorfer Bruch in Brandenburg, Germany. The aim of this study was to investigate whether exposure of maize pollen from surrounding Bt-maize fields to NTOs in the nature protection area could be excluded or not. Two types of exposure were investigated: Firstly, whether maize pollen was dispersed by wind into the nature reserve area exposing resident NTOs. Secondly, foraging NTOs from the nature reserve are exposed by roaming the surrounding fields and collecting maize pollen. In order to fulfil the precautionary principle defined by law, the study should help to determine appropriate isolation distances for the cultivation of Bt-maize with regard to sustainable protection of NTOs in the FFH Ruhlsdorfer Bruch. In 2007, the local authorities issued an isolation distance between Bt-maize fields and nature reserves of 100?m and in 2008, this became 250?m in the northern and 500?m in the westerly direction, respectively. Materials and methods Standardised methods for biological and technical pollen sampling issued by the Association of German Engineers (VDI 4330 Part 3, 2007 and VDI 4330 Part 4, 2006) were applied providing a quality controlled and methodologically harmonised database which does not only serve the needs to be fulfilled by the present case-specific monitoring study but can also be used as a reference database for further investigations. Maize pollen exposure was measured within the FFH Ruhlsdorfer Bruch and its immediate vicinity in July and August 2007 and 2008. In 2007, the sampling was performed at three sites using 12 technical samplers (Sigma-2/PMF) placed at five measuring points at distances from 5?m to 120?m from the maize field edges. Additionally, for biological pollen sampling six bee colonies were situated at these three sites (two colonies at each site). The technical sampler Sigma-2/PMF enables point sampling which is primarily influenced by wind and topography providing information on effective maize pollen input (flow and deposition) at the measuring sites. Honey-bees roaming in the surrounding area with typical foraging distances of several kilometres may act as planar collectors. They may serve as indicators for the exposure to pollen-collecting NTOs. Furthermore, biological sampling is more selective due to the organism’s preferences, whereas the technical sampling is neutral. Hence, both the technical and the biological sampling complement each other in their scope of application. The pollen samples of both matrices were analysed microscopically and the maize pollen loads were quantified. Pollen-DNA was analysed by means of the quantitative PCR-method (qPCR) identifying conventional and Bt-maize pollen by two independent laboratories. In 2008, the monitoring was repeated with additional sites. Eighteen technical samplers were exposed at five sites with eight measuring points at distances from 5?m to 250?m from the maize field edges. Two honey-bee colonies for biological sampling were placed at one site for control purposes. PCR-analyses were performed to measure the amounts of Bt-maize pollen in the samples. Results The results of pollen monitoring at the Ruhlsdorfer Bruch revealed maize pollen exposure for all monitoring sites in both surveys. In 2007, up to 1.75 million maize pollen/m² were deposited at sites closest to the maize field. At 120?m from field edge in the middle of the FFH-reserve, 99,000 maize pollen/m² were detected. In 2008, similar results were found, at distances up to 250?m from the field edges deposition of 164,000 maize pollen/m² was detected. Data on maize pollen deposition show a clear distance relationship and are in accordance with results of further comprehensive surveys based on the same methodology (Hofmann 2007). The results of the microscopic analysis of the pollen pellets demonstrated that bees collected maize pollen at all sites, in 2007 and 2008. Although maize pollen is not the main food source (2007:0.1–0.3?%; 2008:2–3?%) the collection efficiency of the bee colonies resulted in high amounts of sampled maize pollen with 4 to 11 million per site in 2007 and up to 467 million in 2008. Molecular-biological analysis of maize pollen DNA by qPCR demonstrated that transgenic Bt-MON810 DNA was present in all technical and biological samples, corroborated by two independent laboratories. In 2007, the GMO-content in the samples ranged up to 44?% in the bioaerosols and 49?% in the pollen pellets. In 2008, GMO-proportions of up to 18?% were detected. Discussion The results of this study provide evidence that NTOs in the Ruhlsdorfer Bruch were exposed to Bt-maize pollen under the cultivation conditions in 2007 with a buffer zone of 100?m. The GMO-content reached up to 48?%. The results of the monitoring in 2008 confirmed these findings. Even though the exposure could be reduced by increasing the isolation distances to 250?m and 500?m respectively, the results still show percentages of up to 18?% Bt-MON810 in the pollen samples. The results on maize pollen deposition at the Ruhlsdorfer Bruch in 2007 and 2008 correspond to the results of an investigation which was conducted over several years applying the same standardised method, but covering a wider range of distances. The correlation between maize pollen deposition (n/m²) and distance to the source field (m) fitted best to a power function of the type y = 1.2086 · 10⁶ · x ^–0.548. Despite the same trend, the pollen deposition in the Ruhlsdorfer Bruch revealed above-average findings. Also the analysis of the pollen pellets collected by the bees showed an exposure in 2007 with values for the GMO-content of up to 49?%. For both methods, the exposure decreased in 2008 due to the greater buffer zones up to 500?m. Whereas the GMO-content for the biological sampling were reduced to values below 10?%, the values for the technical sampling were still higher indicating that greater buffer zones would be necessary for safety reasons under the precautionary principle. Conclusions The results of this investigation proved that maize pollen were dispersed by wind to distances farther than 250?m from field edge leading to maize pollen exposure in the centre of the nature reserve. The results also demonstrated that foraging NTOs living in the nature reserve were exposed to maize pollen from surrounding fields. Considering the cultivation of Bt-maize MON810, the assumption of the Environmental Risk Assessment (ERA) that there will be no relevant exposure beyond the Bt-maize fields, cannot be confirmed. Considering the results of this and related studies and with respect to the precautionary principle, one can state that buffer zones between Bt-maize fields and protected areas are an effective measure to minimise the exposure of Bt-maize pollen to NTOs and, thus, to prevent from adverse effects. Recommendations and perspectives Because of still insufficient ecotoxicological data for the risk assessment of Bt-maize MON810 considering butterflies and other protected NTOs, protection standards assuring the precautionary principle have to be implemented to avoid Bt-maize pollen exposure to NTOs. This applies for the case Ruhlsdorfer Bruch and for nature reserve areas in general. In order to exclude risks to protected NTOs occurring in nature reserves, sufficient buffer zones for Bt-maize cultivation should be considered. The statistical analysis revealed that distances of more than 500?m are necessary to decisively reduce exposure to foraging insects. In fact, distances of more than 1,000?m are necessary to prevent maize pollen deposition from values above 100,000 pollen/m² with a certainty of 90?%. An adequate risk assessment can only be attained if based on field measurements accounting for the high variation of pollen deposition due to local environmental site conditions and field management. The monitoring should be based on standardised methods. It should include locations with the highest expected deposition rates, the boundaries of the protected areas and sites of interest within those boundaries, e.?g., specific habitats of sensitive species.     

Klima-Biomonitoring: Nachweis des Klimawandels und dessen Folgen für die belebte Umwelt

Hintergrund und ZielEs wird vorgeschlagen, Biomonitoring-Verfahren zu verwenden, um Auswirkungen des Klimawandels auf die belebte Umwelt zu erkennen, zu bewerten und zu dokumentieren, weil ? es mit dieser Methode gelingt, klimainduzierte Veränderungen in besonders empfindlichen Gebieten in Deutschland mit ihren Lebensräumen, Lebensgemeinschaften und Arten darzustellen, ? es bezüglich Zuwanderung und Ausbreitung neuer Schädlinge und Krankheitserreger für Mensch, Tier und Pflanze nach bzw. in Deutschland relevante Informationen liefern kann, ? damit der Politik zur Bewertung der Auswirkungen des Klimawandels wichtige Informationen, Handreichungen und Entscheidungsgrundlagen zur Verfügung gestellt werden können und ? auf dieser Grundlage geeignete Anpassungsmaßnahmen eingeleitet und auf ihre Wirksamkeit geprüft werden können, wie beispielsweise in der Deutschen Anpassungsstrategie (BMU 2009) beschrieben. Für Biomonitoring-Verfahren, die geeignet sind, Auswirkungen des Klimawandels anzuzeigen, wird der Begriff Klima-Biomonitoring vorgeschlagen Diese Verfahren sollten aus verschiedenen Gründen (u.?a. abgestimmte Methodik und gleiche Datenbasis, Kostenersparnis) unter Beteiligung aller Bundesländer umgesetzt werden. Bioindikation ist bereits heute ein unverzichtbares Verfahren, frühzeitig Veränderungen in der belebten Umwelt zu erkennen und somit Hinweise auf besondere Gefahren zu liefern (Frühwarnsystem). Für das Klima-Biomonitoring werden vorzugsweise bestehende Monitoringsysteme mit ihren bereits erhobenen Daten ausgewertet und mitgenutzt. Material und Methoden Das Klima-Biomonitoring greift auf bereits eingeführte und bewährte Methoden der Bioindikation von Umweltveränderungen zurück. Bestehende Methoden werden im Hinblick auf die besonderen, durch den Klimawandel bedingten Anforderungen ergänzt, angepasst und weiterentwickelt. Auf der Grundlage einer Auswertung relevanter laufender Bundes- und Länderprogramme werden wesentliche Wirkungen des Klimawandels identifiziert, die datenliefernden Programme zugeordnet und Auswertungen vorgeschlagen. Zusätzlich werden Datenquellen beschrieben, deren Nutzung weitergehende Betrachtungen ermöglichen. ErgebnisseIn einer Übersichtstabelle werden Monitoring-Programme auf Bundes- und Länderebene systematisiert und hinsichtlich ihrer Bedeutung für die Bewertung von Klimafolgen auf die belebte Umwelt dargestellt. Eine Übersicht über Datenquellen sowie eine Darstellung erster Erkenntnisse aus der Auswertung vorliegender Daten belegen die Relevanz des vorgeschlagenen Verfahrens. Der erkennbare Forschungs- und Entwicklungsbedarf wird umrissen und Vorschläge für eine Optimierung des Daten- und Methodenaustausches benannt. Beispiele zur Anwendung und Vertiefung der Thematik sowie Anregungen zur Weiterentwicklung der Methodik und zum Schließen von Kenntnislücken werden in Folgepublikationen aufgezeigt. DiskussionAuf der Basis des Klima-Biomonitorings kann das Ausmaß der durch den Klimawandel bereits eingetretenen Veränderungen beschrieben sowie Szenarien und Prognosen zu den Auswirkungen von Klimaveränderungen erstellt werden. Weiterhin ist es möglich, die sekundären Wirkungen des Klimawandels, insbesondere die Auswirkungen der Maßnahmen zur Anpassung an den Wandel, mithilfe der Bioindikation auf ihre Wirksamkeit zu prüfen. Für einige Klima-Bioindikatoren ist der kausale Zusammenhang zwischen Klimawandel und Reaktion bereits belegt (zum Beispiel die Frühjahrsphasen in der Pflanzenphänologie), in anderen Fällen müssen noch Methoden zur Unterscheidung zwischen Klimawirkungen und anderen Wirkfaktoren entwickelt werden. Die Erkenntnisse aus dem Klima-Biomonitoring sollen Grundlage für entsprechende Handlungen sein, sodass geeignete Anpassungsstrategien und gleichzeitig Maßnahmen zur Vermeidung oder zur Verminderung der Effekte eingeleitet werden können. Zugleich sollen eine angemessene Politikberatung, eine Information der Öffentlichkeit und die Erfüllung entsprechender Berichtspflichten erfolgen. Schlussfolgerungen:Klima-Biomonitoring ist eine geeignete Methode, um klimainduzierte Veränderungen in besonders empfindlichen Gebieten in Deutschland mit ihren Lebensräumen, Lebensgemeinschaften und Arten darzustellen. Erste Auswertungen zeigen, dass bereits Wirkungen des Klimawandels auf die belebte Umwelt nachweisbar sind. Hierbei liefert die Pflanzenphänologie seit vielen Jahren wertvolle Hintergrunddaten. Eine Verschneidung dieser Hintergrunddaten mit Daten aus anderen geeigneten Monitoring-Programmen und weiteren Informationen (zum Beispiel Geobasisdaten) ermöglicht es, diese Erkenntnisse zu ergänzen. Damit ist die Ermittlung und Bewertung von klimainduzierten Wirkungen auch im Bereich der Ausbreitung von Krankheitsüberträgern und -erregern und anderer Phänomene sowie der Bewertung von Maßnahmen möglich. Empfehlungen und PerspektivenEine abgestimmte Anwendung der Methodik in allen Bundesländern und beim Bund, eine Verbesserung des Daten- und Methodenaustausches, die Identifikation, Erschließung und Nutzung weiterer Datenquellen, die Weiterentwicklung der Methodik und eine Publikation weiterer Erkenntnisse werden empfohlen. Es ist davon auszugehen, dass sich das Klima-Biomonitoring wegen seiner Vorteile als Methode zur Erhebung von Klimafolgen für die belebte Umwelt auch international etablieren wird.