4种不同生境的蟹类金属硫蛋白cDNA的克隆与比较

利用Carcinus maenas金属硫蛋白氨基酸序列资料，用全简并引物从鳃组织总RNA中扩增并克隆了首个甲壳类金属硫蛋白cDNA片段序列，3'-RACE获得了其编码区全长cDNA。之后，用部分简并的引物扩增并克隆了其它3种国内常见蟹类的金属硫蛋白cDNA编码区全长序列。序列分析结果表明，几种蟹的金属硫蛋白cDNA序列存在差异，推知的氨基酸序列也不完全相同，比较不同蟹的cDNA和氨基酸序列数据不能证明不同生态环境对金属硫蛋白的分子进化起重要作用。图4表1参15     

我国人口资源与食物问题

人口增多,耕地减少,部分地区供水不足,是我国人口与资源矛盾的基本格局。由于人口的压力,对资源实行超强度的利用,使较大范围的地区生态环境恶化,严重威胁农业生产,并成为许多地区多灾、低产、贫困的根本原因,较低的食物人均占有水平,将继续成为中国国民经济发展和人民生活水平提高的严重限制因素。增加食物,必须挖掘资源的潜力,开源与节流相结合,以内涵挖潜为主。首先是要立足现有的耕地,致力于提高单产;同时,合理开发水域、山地、草地等资源,广辟食物来源,提高非耕地资源的生产力,提高林牧渔业的发展水平。耕地应以深度开发为主,走资源节约型(节地、节水、节时、节能)的集约化道路。     

Exploration of Science Parks

1 DEVELOPMENT OF SCIENCE PARKSThe concept of science park originated in the US. Manyterms are used to describe science parks, such as researchpark, technology park, science centre, research centre,innovation centre, and with various combination of these(MacDonald, 1987). The first science park in the worldis Stanford Industrial Park established in 1951. In 1955,only seven companies were located in the park. By 1980there were ninety companies including Hewlett PackardCompany, whic…     

Evaluation and analysis of heavy metals in iron and steel industrial area

Environment, Development and Sustainability - Heavy metal pollution has attracted more attention due to the toxicity and migration characteristics, which has close relationship with soil...     

大纵坡城市道路透水边带截留能力试验

道路是城市重要的交通纽带,一旦发生积水内涝,将导致城市交通瘫痪,甚至危及生命财产安全,特别是山地城市道路普遍存在纵坡大、雨水口截留效率低等突出问题,发生积水内涝和马路洪水的风险较高。针对上述问题,提出了利用透水边带提高大纵坡城市道路雨水径流截留效率的方法。在实验室搭建了城市单车道物理试验模型(比例1∶1),采用人工模拟降雨方法,系统研究了不同透水面积比、不同重现期降雨条件下透水边带对雨水径流的截留效率,并与传统道路雨水口截留能力进行了比较。试验结果表明:相同透水面积比条件下,雨水径流截留能力随着重现期的增大而减小;相同重现期条件下,雨水径流截留能力随着透水面积比的增加而增大,在重现期P=5 a时,透水边带面积比从12.5%增加到50%,雨水径流截留率从72.3%增加到79.3%。与传统雨水口截留能力相比,增加透水边带后雨水径流截留能力可提高30%左右。因此,大纵坡城市道路在不影响交通安全的条件下,可根据道路空间布局特征,通过设置透水边带来提高雨水径流的截流效率。     

基于“BIM+GIS”技术的建筑垃圾精准管控信息管理平台研究初探

近年来,我国建筑垃圾产量不断增加,全国600多座大中城市中有70%被垃圾所包围,其中建筑垃圾贡献率达到40%,而相比于部分发达国家,我国尚缺少针对于建筑垃圾的全过程实时监测与智能管控手段。将"BIM+GIS"技术引入建筑垃圾监管领域,并基于此技术建立建筑垃圾实时监测与智能管控信息管理平台,可实现对建筑垃圾的精准管控。     

Performance of different macrophytes in the decontamination of and electricity generation from swine wastewater via an integrated constructed wetland-microbial fuel cell process

Plants constitute a major element of constructed wetlands(CWs).In this study,a coupled system comprising an integrated vertical flow CW(IVCW) and a microbial fuel cell(MFC) for swine wastewater tre atment was developed to research the effects of macrophytes commonly employed in CWs,Canna indica,Acorus calamus,and Ipomoea aquatica,on decontamination and electricity production in the system.Because of the different root types and amounts of oxygen released by the roots,the rates of chemical oxygen demand(COD) and ammonium nitrogen(NH_4~+-N) removal from the swine wastewater differed as well.In the unplanted,Canna indica,Acorus calamus,and Ipomoea aquatica systems,the COD removal rates were 80.20%,88.07%,84.70%,and 82.20%,respectively,and the NH_4~+-N removal rates were 49.96%,75.02%,70.25%,and 68.47%,respectively.The decontamination capability of the Canna indica system was better than those of the other systems.The average output voltages were 520±42,715±20,660±27,and 752±26 mV for the unplanted,Canna indica,Acorus calamus,and Ipomoea aquatica systems,respectively,and the maximum power densities were 0.2230,0.4136,0.3614,and0.4964 W/m~3,respectively.Ipomoea aquatica had the largest effect on bioelectricity generation promotion.In addition,electrochemically active bacteria,Geobacter and Desulfuromonas,were detected in the anodic biofilm by high-throughput sequencing analysis,and Comamonas(Proteobacteria),which is widely found in MFCs,was also detected in the anodic biofilm.These results confirmed the important role of plants in IVCW-MFCs.     

Dynamic nano-Ag colloids cytotoxicity to and accumulation by Escherichia coli: Effects of Fe3+, ionic strength and humic acid

Released Ag ions or/and Ag particles are believed to contribute to the cytotoxicity of Ag nanomaterials, and thus, the cytotoxicity and mechanism of Ag nanomaterials should be dynamic in water due to unfixed Ag particle:Ag⁺ ratios. Our recent research found that the cytotoxicity of PVP-Ag nanoparticles is attributable to Ag particles alone in 3 hr bioassays, and shifts to both Ag particles and released Ag⁺ in 48 hr bioassays. Herein, as a continued study, the cytotoxicity and accumulation of 50 and 100 nm Ag colloids in Escherichia coli were determined dynamically. The cytotoxicity and mechanisms of nano-Ag colloids are dynamic throughout exposure and are derived from both Ag ions and particles. Ag accumulation by E. coli is derived mainly from extracellular Ag particles during the initial 12 hr of exposure, and thereafter mainly from intracellular Ag ions. Fe³⁺ accelerates the oxidative dissolution of nano-Ag colloids, which results in decreasing amounts of Ag particles and particle-related toxicity. Na⁺ stabilizes nano-Ag colloids, thereby decreasing the bioavailability of Ag particles and particle-related toxicity. Humic acid (HA) binds Ag⁺ to form Ag⁺-HA, decreasing ion-related toxicity and binding to the E. coli surface, decreasing particle-related toxicity. HA in complex conditions showed a stronger relative contribution to toxicity and accumulation than Na⁺ or Fe³⁺. The results highlighted the cytotoxicity and mechanism of nano-Ag colloids are dynamic and affected by environmental factors, and therefore exposure duration and water chemistry should be seriously considered in environmental and health risk assessments.     

Synergistic effects of Cu species and acidity of Cu-ZSM-5 on catalytic performance for selective catalytic oxidation of n-butylamine

In this work, a series of Cu-ZSM-5 catalysts with different SiO₂/Al₂O₃ ratios (25, 50, 100 and 200) were synthesized and investigated in n-butylamine catalytic degradation. The n-butylamine can be completely catalytic degradation at 350°C over all Cu-ZSM-5 catalysts. Moreover, Cu-ZSM-5 (25) exhibited the highest selectivity to N₂, exceeding 90% at 350°C. These samples were investigated in detail by several characterizations to illuminate the dependence of the catalytic performance on redox properties, Cu species, and acidity. The characterization results proved that the redox properties and chemisorption oxygen primarily affect n-butylamine conversion. N₂ selectivity was impacted by the Brønsted acidity and the isolated Cu²⁺ species. Meanwhile, the surface acid sites over Cu-ZSM-5 catalysts could influence the formation of Cu species. Furthermore, in situ diffuse reflectance infrared Fourier transform spectra was adopted to explore the reaction mechanism. The Cu-ZSM-5 catalysts are the most prospective catalysts for nitrogen-containing volatile organic compounds removal, and the results in this study could provide new insights into catalysts design for VOC catalytic oxidation.     

UV/H2O2 oxidation of tri(2-chloroethyl) phosphate: Intermediate products, degradation pathway and toxicity evaluation

Tri(2-chloroethyl) phosphate (TCEP) with the initial concentration of 5 mg/L was degraded by UV/H₂O₂ oxidation process. The removal rate of TCEP in the UV/H₂O₂ system was 89.1% with the production of Cl^? and PO₄^3? of 0.23 and 0.64 mg/L. The removal rate of total organic carbon of the reaction was 48.8% and the pH reached 3.3 after the reaction. The oxidative degradation process of TCEP in the UV/H₂O₂ system obeyed the first order kinetic reaction with the apparent rate constant of 0.0025 min^?1 (R²=0.9788). The intermediate products were isolated and identified by gas chromatography-mass spectrometer. The addition reaction of HO? and H₂O and the oxidation reaction with H₂O₂ were found during the degradation pathway of 5 mg/L TCEP in the UV/H₂O₂ system. For the first time, environment risk was estimated via the “ecological structure activity relationships” program and acute and chronic toxicity changes of intermediate products were pointed out. The luminescence inhibition rate of photobacterium was used to evaluate the acute toxicity of intermediate products. The results showed that the toxicity of the intermediate products increased with the increase of reaction time, which may be due to the production of chlorine compounds. Some measures should be introduced to the UV/H₂O₂ system to remove the highly toxic Cl-containing compounds, such as a nanofiltration or reverse osmosis unit.