Distribution of atrazine in a crop-soil-groundwater system at Baiyangdian Lake area in China

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The use of ultraviolet germicidal irradiation (UVGI) in disinfection of airborne bacteria

Contamination of indoor air by microbial pollutants has been increasingly recognized as a public health problem, and may be responsible for building-related illness (BRI) and sick-building syndrome (SBS). Bioaerosols such as fungi, bacteria, and viruses in indoor air can cause allergic and irritant responses, infectious diseases, respiratory problems, and hypersensitivity reactions. People sensitive to indoor environmental problems complain of a wide variety of symptoms ranging from headache, fatigue, nausea, sinus congestion, to eye, nose, and throat irritations.Although ultraviolet germicidal irradiation (UVGI) with a predominant wavelength of 254 nm has been used for air disinfection for many years to minimize microbial numbers in the air, few quantitative data are available on the radiation susceptibilities of individual airborne microbes. There have been a number of UVGI studies documenting the effectiveness of UVGI for the control of microbes in controlled settings. Many of these studies documented the effectiveness of UVGI against airborne tuberculosis organisms.The studies described here used commercial type fan-powered shielded UVGI-containing fixtures to evaluate their effectiveness in air disinfection. Aerosolization tests were done in the contained environment of a negative pressure bioaerosol stainless steel testing chamber 0.75 m wide·3.7 m long. The chamber was designed so that microbes could be safely aerosolized and contained while traversing the chamber through the UVGI-containing fix-tures. Four commercial (Purair UV Germicidal Systems, Mount Vernon, N.Y.) fan-powered UVGI-containing fix-tures placed in the chamber were individually evaluated for their ability to disinfect individual bioaerosols of air-borne bacteria.Air samples were taken at the inlet and outlet of the UVGI-containing units positioned in the bioaerosol chamber, using Ace Glass all-glass impingers (AGI-30). Five bacterial species were individually aerosolized to evaluate their kill. The bacteria used to test all of the UVGI-containing units were vegetative cells of Escherichia coli, Micrococcus luteus, Pseudomonas fluorescens, Staphylococcus aureus, and endospores of Bacillus subtilis. Based upon the concentration of bioaerosols collected at the inlet and outlet of the fixtures tested, the total overall microbial kills for the four fixtures with the filters in place and the UVGI units on were more than 99% for all the airborne vegetative bacteria tested, and a mean of over 75% for the B. subtilis endospores.All of the fixtures were efficient in the kill of the test vegetative bacteria used, even the more UVGI-resistant M. luteus vegetative cells and endospores of B. subtilis. Units such as these may provide an economical way to supplement existing air cleaning procedures used in indoor environments, and to kill airborne bacteria effectively without human exposure to UV light.     

Carbon mitigation potential and costs of forestry options in Brazil,China, India,Indonesia, Mexico,the Philippines and Tanzania

This paper summarizes studies of carbon (C) mitigation potential and costs of about 40 forestry options in seven developing countries. Each study uses the same methodological approach – Comprehensive Mitigation Assessment Process (COMAP) – to estimate the above parameters between 2000 and 2030. The approach requires the projection of baseline and mitigation land-use scenarios. Coupled with data on a per ha basis on C sequestration or avoidance, and costs and benefits, it allows the estimation of monetary benefit per Mg C, and the total costs and carbon potential. The results show that about half (3.0 Pg C) the cumulative mitigation potential of 6.2 Petagram (Pg) C between 2000 and 2030 in the seven countries (about 200× 10⁶ Mg C yr^-1) could be achieved at a negative cost and the remainder at costs ranging up to $100 Mg C^-1. About 5 Pg C could be achieved, at a cost less than $20 per Mg C. Negative cost potential indicates that non-carbon revenue is sufficient to offset direct costs of these options. The achievable potential is likely to be smaller, however, due to market, institutional, and sociocultural barriers that can delay or prevent the implementation of the analyzed options.     

Cytogenetic analysis of trophoblasts by comparative genomic hybridization in embryo-fetal development anomalies

Cytogenetic studies of spontaneous abortions or intrauterine fetal death depend on conventional tissue culturing and karyotyping. This technique has limitations such as culture failure and selective growth of maternal cells. Fluorescent in situ hybridization (FISH) using specific probes permits diagnosis of aneuploidies but is limited to one or a few chromosomal regions. Comparative genomic hybridization (CGH) provides an overview of chromosomal gains and losses in a single hybridization directly from DNA samples. In a prospective study, we analyzed by CGH trophoblast cells from 21 fetuses in cases of spontaneous abortions, intrauterine fetal death or polymalformed syndrome. Six numerical chromosomal abnormalities including one trisomy 7, one trisomy 10, three trisomies 18, one trisomy 21 and one monosomy X have been correctly identified by CGH. One structural abnormality of the long arm of chromosome 1 has been characterized by CGH. One triploidy and two balanced pericentromeric inversions of chromosome 9 have not been identified by CGH. Sexual chromosomal constitutions were concordant by both classical cytogenetic technique and CGH. Contribution of trophoblast analysis by CGH in embryo-fetal development anomalies is discussed. Copyright © 2001 John Wiley & Sons, Ltd.     

Preimplantation genetic diagnosis for familial amyloidotic polyneuropathy (FAP)

Preimplantation genetic diagnosis (PGD) was developed more than a decade ago to offer an alternative to prenatal diagnosis for couples at risk of transmitting an inherited disease to their offspring. Portuguese-type familial amyloidotic polyneuropathy (FAP type I), is an autosomal dominant disease presenting an inherited mutation in the gene encoding the plasma protein transthyretin (TTR). We here report the first protocol for single-cell detection of the Met30 mutation in FAP type I and its application to PGD. A nested PCR reaction for exon 2 of the TTR gene was developed. The PCR product was then analysed by restriction enzyme analysis and SSCP allowing the detection of the point mutation. Ten clinical cycles were performed in seven couples. From the 93 metaphase II (MII) injected oocytes, 82 were normally fertilized and 78 were biopsied. A positive signal in the nested PCR reaction was obtained in 61 blastomeres, corresponding to a DNA amplification efficiency of 78.2%. No allele dropout (ADO) or contamination were detected. A biochemical pregnancy was obtained in three cases and a clinical pregnancy in one couple is actually in normal evolution. Copyright © 2001 John Wiley & Sons, Ltd.     

Denaturing high-performance liquid chromatography (DHPLC)-based prenatal diagnosis for tuberous sclerosis

Tuberous sclerosis (TSC) is a frequent autosomal-dominant condition (affecting 1 in 6000 individuals) caused by various mutations in either the hamartin (TSC1) or the tuberin gene (TSC2). This allelic and non-allelic heterogeneity makes genetic counseling and prenatal diagnosis difficult, especially as a significant proportion of TSC cases are due to de novo mutations. For this reason the identification of the disease causing mutation is mandatory for accurate counseling, yet current mutation detection methods such as single-strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE) are labor intensive with limited detection efficiency. Denaturing high-performance liquid chromatography (DHPLC) is a high-throughput, semi-automated mutation detection system with a reported mutation detection rate close to 100% for PCR fragments of up to 800 bp. We used a recently described DHPLC assay allowing the efficient detection of mutations in TSC1 to analyze the DNA extracted from a chorion villus sample in order to perform a prenatal diagnosis for TSC. The fetus was found not to have inherited the deleterious mutation and the DHPLC diagnosis was confirmed by haplotype analysis. This represents the first DHPLC-based prenatal diagnosis of a genetic disease. Copyright © 2001 John Wiley & Sons, Ltd.     

Pulmonary stenosis and reactive right ventricular hypertrophy in the recipient fetus as a consequence of twin-to-twin transfusion

The present study describes an association between adverse outcome in the twin-to-twin transfusion syndrome (TTTS) and pulmonary stenosis or reactive right ventricular hypertrophy. Six discordant monozygotic twin pregnancies with TTTS are described. Ventricular hypertrophy and atrioventricular valvular regurgitation occurred in all the recipient twins with pulmonary valvular stenosis in three cases and infundibular stenosis in one case. The recipient twin in one pair and both twins in another pregnancy died as a consequence of immaturity but the remaining twins all survived. Surgical intervention was required in one baby for valvular pulmonary stenosis. Our observations suggest that elevated blood pressure in the transfusion recipient may play an important role in pathogenesis. We hypothesise that both pulmonary stenosis and right chamber hypertrophy are secondary to hemodynamic changes. Although we have found valvular pulmonary stenosis in three recipients and infundibular stenosis in only one, this (obstruction to outflow) could be due to right chamber hypertrophy. Copyright © 2001 John Wiley & Sons, Ltd.     

Restrictive dermopathy and fetal behaviour

We report three siblings from consecutive pregnancies affected with restrictive dermopathy (RD). During the second pregnancy, fetal behavioural development and growth were studied extensively using ultrasound at 1–4 week intervals. Dramatic and sudden changes occurred in fetal body movements and growth but not until the end of the second trimester of pregnancy. Prominent at that time were prolonged periods of fetal quiescence and very low heart rate variability, together with abnormally executed body movements of short duration. Retarded femoral development and jerky abrupt fetal body movements (abnormal movement quality) were already present in the early second trimester of pregnancy. Facial anomalies emerged despite the presence of fetal mouth movements. The clinical features of RD were only partly explained by present knowledge of skin development and the fetal akinesia deformation sequence hypothesis. Quantitative assessment of fetal movements proved to be a poor early marker for antenatal diagnosis of this disorder. Copyright © 2001 John Wiley & Sons, Ltd.     

过去50年中国森林资源和降水变化的统计分析

论文对过去50年中国森林资源和降水变化进行了统计分析，以期在大尺度上寻找森林资源与降水变化之间的关系。主要结论为：①建国以来我国的森林覆盖率呈上升趋势；②过去50年全国、林区、非林区降水量均呈减少趋势，干旱化趋势明显；③对建国后历次森林资源调查（清查）时段内降水差值（以百分数为单位）、森林覆盖率（以相邻两次变化百分数为单位）变化关系的分析表明，二者的统计关系并不显著，即：过去50年我国森林资源的变化对降水没有显著的影响；④森林影响降水是一个复杂的系统问题，该研究还存在着许多不确定性因素，需进一步实验验证与理论探讨。