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LCAs (life cycle assessments) are often based on average data to produce a generic evaluation of a good or service. However, ignoring variability and induced uncertainty of LCA results reduces their significance, especially when dealing with agricultural processes that present high natural fluctuations. The objective of the study was to explore the robustness of LCA results when accounting for variable emissions data, illustrated by the case of slurry application techniques. Four application techniques were compared: band spreading, broadcast spreading, harrowing after surface application and direct injection.On the basis of the normalisation results, acidification, eutrophication and global warming potentials were selected. To estimate field nitrogen emissions, an original approach was developed based on relative nitrogen loss factors for each technique from a literature review. The calculated field emissions from different soil and climate conditions were considered equally probable and were propagated into a range of LCA result using the Monte Carlo method. Injection and harrowing both showed reduced acidification and eutrophication potentials compared to band spreading and broadcast spreading but had larger global warming potentials, which could be particularly important with injection. Harrowing consequently appeared as the best compromise. Despite the large range of LCA results, robust conclusions could be drawn. To achieve a more refined comparison between the techniques, the use of process-based models in contrasted situations is suggested.  相似文献   
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In its successful annual cycle of controversies and debates, the International Society of Prenatal Diagnosis and Therapy once again addressed non-invasive prenatal testing (NIPT) by following up on the 2013 controversy, ‘Should non-invasive DNA testing be the standard screening test for Down syndrome in all pregnant women’? with the proposition, ‘NIPT for chromosomel abnormalities should be offered to women with low a priori risk’. © 2014 John Wiley & Sons, Ltd.  相似文献   
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The public perception of climate change is characterized by heterogeneity, even polarization. Deliberative discussion is regarded by some as key to overcoming polarization and engaging various publics with the complex issue of climate change. In this context, online engagement with news stories is seen as a space for a new “deliberative democratic potential” to emerge. This article examines aspects of deliberation in user comment threads in response to articles on climate change taken from the Guardian. “Deliberation” is understood through the concepts “reciprocity”, “topicality”, and “argumentation”. We demonstrate how corpus analysis can be used to examine the ways in which online debates around climate change may create or deny opportunities for multiple voices and deliberation. Results show that whilst some aspects of online discourse discourage alternative viewpoints and demonstrate “incivility”, user comments also show potential for engaging in dialog, and for high levels of interaction.  相似文献   
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During composting, the degradation of organic waste is accompanied and driven by a succession of microbial populations exhibiting a broad range of functional capabilities. Detailed inventories of the microbial communities in mature compost, however, are not available. Mature composts, originating from biowaste as well as sewage sludge and anaerobic sludge, were studied by denaturing gradient gel electrophoresis-fingerprints after polymerase chain reaction (PCR) amplification of the 16S rRNA genes using three different universal primer pairs, as well as by differential scanning calorimetry and thermogravimetry. The composts of different origin had different bacterial communities. The influence of different 16S rDNA primer sets on the same batches of compost DNA was evaluated. The clearest separation of different compost types was obtained by using the PCR primer pair 338f + 518r which is suggested for future applications. Communities from the different biowaste compost samples clustered together and could be separated from sewage sludge communities indicating the establishment of different microbial consortia. A similar differentiation of composts was found with the thermogavimetric analyses. It may thus be concluded that the resulting humus quality is closely linked to the microbial communities involved.  相似文献   
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The degree of association between industrial odours and reported annoyance was investigated by means of field studies in four German cities, namely Duisburg (n = 400), Brühl (n = 539), Dortmund (n = 400) and Rodenkirchen (n = 200). The degree of odour exposure was assessed by estimating the frequency of odours by means of systematic field observations, whereas annoyance was assessed by personal interviews using standardized questionnaires. Both studies revealed highly significant associations between odour exposure and degree of annoyance. Odour annoyance was correlated with personal factors: age was negatively related to annoyance, whereas dissatisfaction with health exhibited positive associations with annoyance. Coping strategies were also associated with odour annoyance: high scores in problem-oriented coping strategies increased annoyance, whereas high scores in avoidance coping reduced annoyance. Coping strategies based upon the reduction of emotional stress were not associated with annoyance. Since none of the interaction terms of the full regression model proved significant, no true moderators were identified. In order to check for aquiescence bias, people who were interviewed personally were compared with those who could not be approached directly and who were therefore contacted by mail. Despite some significant differences in terms of gender, education, occupation, problem-oriented coping and avoidance coping, both groups did not differ in terms of annoyance.  相似文献   
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