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Toxicity, dioxin-like activities, and endocrine effects of DDT metabolites—DDA, DDMU, DDMS, and DDCN
Wetterauer B Ricking M Otte JC Hallare AV Rastall A Erdinger L Schwarzbauer J Braunbeck T Hollert H 《Environmental science and pollution research international》2012,19(2):403-415
Background, aim, and scope
2,2-bis(chlorophenyl)-1,1,1-trichloroethane (DDT) metabolites, other than those routinely measured [i.e., 2,2-bis(chlorophenyl)-1,1-dichloroethylene (DDE) and 2,2-bis(chlorophenyl)-1,1-dichloroethane (DDD)], have recently been detected in elevated concentrations not only in the surface water of Teltow Canal, Berlin, but also in sediment samples from Elbe tributaries (e.g., Mulde and Havel/Spree). This was paralleled by recent reports that multiple other metabolites could emerge from the degradation of parent DDT by naturally occurring organisms or by interaction with some heavy metals. Nevertheless, only very few data on the biological activities of these metabolites are available to date. The objective of this communication is to evaluate, for the first time, the cytotoxicity, dioxin-like activity, and estrogenicity of the least-studied DDT metabolites. 相似文献33.
Hinger G Brinkmann M Bluhm K Sagner A Takner H Eisenträger A Braunbeck T Engwall M Tiehm A Hollert H 《Environmental science and pollution research international》2011,18(8):1297-1304
Purpose
Heterocyclic aromatic compounds containing nitrogen, sulfur, or oxygen heteroatoms (NSO-HET) have been detected in air, soil, marine, and freshwater systems. However, only few publications are available investigating NSO-HET using in vitro bioassays. To support better characterization of environmental samples, selected NSO-HET were screened for dioxin-like activity in two bioassays. 相似文献34.
Krisztina Vincze Martin Gehring Thomas Braunbeck 《Environmental science and pollution research international》2014,21(13):8233-8241
2,4,7,9-tetramethyl-5-decyne-4,7-diol (TMDD) is a high-production volume chemical used in paper, ink, pesticide, and adhesive industries as a wetting and anti-foaming agent. The physicochemical properties and slow biodegradation rate of TMDD indicate a low bioaccumulation potential but a high prevalence in the environment. As a consequence, TMDD has been detected in several European rivers in the nanogram per liter and lower microgram per liter range; however, its environmental risk to aquatic organisms is considered low. Recent studies almost exclusively focused on acute effects by TMDD, little is known about cytotoxic and genotoxic effects, reproduction and developmental toxicity, endocrine disruption, and any kind of long-term toxicity and carcinogenicity so far. The present study aims to provide more specific baseline information on the ecotoxicological effects of TMDD in fish. For this end, cyto- and genotoxicity assays were carried out in vitro with the permanent fish cell line RTL-W1; in addition, in vivo studies were conducted with the early life stages of zebrafish (Danio rerio) in order to fill the data gaps in developmental toxicity and endocrine disruption. TMDD showed a cytotoxic and slight genotoxic potential in fish cell lines; moreover, various sublethal and lethal effects could be detected in developing zebrafish embryos. There was no evidence of endocrine-disrupting effects by TMDD; however, mortality following prolonged exposure to TMDD during fish sexual development test was clearly higher than mortality in the fish embryo test after 96-h exposure. Our results thus confirmed previous findings of laboratory screening tests, suggesting short-term toxic effects of TMDD in the intermediate, and long-term effects in the lower milligram per liter range. 相似文献
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Keiter S Rastall A Kosmehl T Wurm K Erdinger L Braunbeck T Hollert H 《Environmental science and pollution research international》2006,13(5):308-319
Goals, Scope and Background Fish populations, especially those of the grayling (Thymallus thymallus), have declined over the last two decades in the
upper Danube River between Sigmaringen and Ulm, despite intensive and continuous stocking and improvement of water quality
since the 1970s. Similar problems have been reported for other rivers, e.g. in Switzerland, Great Britain, the United States
and Canada. In order to assess if ecotoxicological effects might be related to the decline in fish catch at the upper Danube
River, sediment, suspended matter and waste water samples from sewage treatment plants were collected at selected locations
and analyzed in a bioanalytical approach using a battery of bioassays. The results of this pilot study will be used to decide
if a comprehensive weight-of-evidence study is needed.
Methods Freeze-dried sediments and suspended particulate matters were extracted with acetone in a Soxhlet apparatus. Organic pollutants
from sewage water were concentrated using XAD-resins. In order to investigate the ecotoxicological burden, the following bioassays
were used: (1) neutral red assay with RTL-W1 cells (cytotoxicity), (2) comet assay with RTLW1 cells (genotoxicity), (3) Arthrobacter
globiformis dehydrogenase assay (toxicity to bacteria), (4) yeast estrogen screen assay (endocrine disruption), (5) fish egg
assay with the zebrafish (Danio rerio; embryo toxicity) and (6) Ames test with TA98 (mutagenicity).
Results and Discussion The results of the in vitro tests elucidated a considerable genotoxic, cytotoxic, mutagenic, bacteriotoxic, embryotoxic and
estrogenic burden in the upper Danube River, although with a very inhomogeneous distribution of effects. The samples taken
from Riedlingen, for example, induced low embryo toxicity, but the second highest 17β-estradiol equivalent concentration (1.8
ng/L). Using the fish egg assay with native sediments, a broad range of embryotoxic effects could be elucidated, with clear-cut
dose-response relationships for the embryotoxic effects of contaminated sediments. With native sediments, embryotoxicity was
clearly higher than with corresponding pore waters, thus corroborating the view that – at least for fish eggs – the bioavailability
of particle-bound lipophilic substances in native sediments is higher than generally assumed. The effect observed most frequently
in the fish egg assay was a developmental delay. A comparison of our own results with locations along the rivers Rhine and
Neckar demonstrated similar or even higher ranges of ecotoxicological burdens in the Danube River.
Conclusions The complex pattern of ecotoxicological effects caused by environmental samples from the Danube River, when assessed in an
in vitro biotest battery using both acute and more specific endpoints, showed that integration of different endpoints is essential
for appropriate hazard assessment. Overall, the ecotoxicological hazard potential shown has indeed to be considered as one
potential reason for the decline in fish catches at the upper Danube River. However, based on the results of this pilot study,
it is not possible to elucidate that chemically induced alterations are responsible for the fish decline.
Recommendations and Perspective . In order to confirm the ecological relevance of the in vitro results for the situation in the field and especially for the
decline of the grayling and other fishes, further integrated investigations are required. For linking the weight of evidence
obtained by in vitro assays and fish population investigations, the application of additional, more specific biomarkers (e.g.
vitellogenin induction, EROD and micronucleus assay) has been initiated in fish taken from the field as well as in situ investigations. 相似文献
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Markus Ulrich Tobias Schulze Erik Leist Bernhard Glaß Matthias Maier Dietrich Maier Thomas Braunbeck Henner Hollert 《Environmental Sciences Europe》2002,14(3):132-137
The potential risk of ground-water contamination through the infiltration of contaminants originating from suspended matter and sediments deposited within flood retention areas in the Upper Rhine valley, as well as subsequent problems for drinking water production, were analyzed by means of the bacterial dehydrogenase activity (DHA; sediment contact test) assay with Arthrobacter globiformis and in a prolonged embryo toxicity assay using zebrafish (Danio rerio). Since the direct uptake of contaminants from particle-bound sources has usually been assumed to play only a minor role in bioaccumulation, the availability of organic extracts to biota has generally been regarded as being overestimated. In order to confirm or discard this hypothesis, organic (acetone) extracts of recently suspended, freeze-dried matter, as well as freeze-dried sediments collected from Rhine flood plains (i.e. soil samples) were compared to corresponding native samples in order to screen for the relative availability of particle-bound contaminants. Whereas a significantly higher ecotoxicological burden was observed for some native soil samples, organic extracts of suspended matter proved to be more toxic than corresponding native samples when compared to organic extracts using each test system. With respect to organic extracts, the suspended matter samples were more toxic than soil samples taken from a rarely flooded site. In contrast to the native samples, the rarely flooded soils revealed a higher toxicity than suspended matter. Therefore, organic extracts did not necessarily overestimate the toxicity of soil and suspended matter samples. Moreover, considerable toxicity of particle-bound contaminants to both bacteria and fish embryos, even in native samples, corroborate the hypothesis that particle-bound contaminants may be more readily available than is generally assumed. 相似文献
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