Invasive cordgrass modifies wetland trophic function

Vascular plants strongly control belowground environments in most ecosystems. Invasion by vascular plants in coastal wetlands, and by cordgrasses (Spartina spp.) in particular, are increasing in incidence globally, with dramatic ecosystem-level consequences. We examined the trophic consequences of invasion by a Spartina hybrid (S. alterniflora x S. foliosa) in San Francisco Bay (USA) by documenting differences in biomass and trophic structure of benthic communities between sediments invaded by Spartina and uninvaded sediments. We found the invaded system shifted from an algae-based to a detritus-based food web. We then tested for a relationship between diet and tolerance to invasion, hypothesizing that species that consume Spartina detritus are more likely to inhabit invaded sediments than those that consume surface algae. Infaunal diets were initially examined with natural abundance stable isotope analyses and application of mixing models, but these yielded an ambiguous picture of food sources. Therefore, we conducted isotopic enrichment experiments by providing 15N-labeled Spartina detritus both on and below the sediment surface in areas that either contained Spartina or were unvegetated. Capitellid and nereid polychaetes, and oligochaetes, groups shown to persist following Spartina invasion of San Francisco Bay tidal flats, took up 15N from labeled native and invasive Spartina detritus. In contrast, we found that amphipods, bivalves, and other taxa less tolerant to invasion consumed primarily surficial algae, based on 13C enrichment experiments. Habitat (Spartina vs. unvegetated patches) and location of detritus (on or within sediments) did not affect 15N uptake from detritus. Our investigations support a "trophic shift" model for ecosystem response to wetland plant invasion and preview loss of key trophic support for fishes and migratory birds by shifting dominance to species not widely consumed by species at higher trophic levels.     

Antioxidant metabolism of coffee cell suspension cultures in response to cadmium

The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to cadmium (Cd) were investigated. Cd accumulated very rapidly in the cells and this accumulation was directly correlated with an increase in applied CdCl(2) concentration in the external medium. At 0.05mM CdCl(2), growth was stimulated, but at 0.5mM CdCl(2), the growth rate was reduced. An alteration in activated oxygen metabolism was detected by visual analysis as well as by an increase in lipid peroxidation at the higher CdCl(2) concentration. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2) and superoxide dismutase (SOD; EC 1.15.1.1) activity increased, particularly at the higher concentration of CdCl(2). Ascorbate peroxidase (APX; EC 1.11.1.11) activity was increased at the lower CdCl(2) concentration used, but could not be detected in cells growing in the higher CdCl(2) concentration after 24h of growth, whilst guaiacol peroxidase (GOPX; EC 1.11.1.7) did not show a clear response to Cd treatment. An analysis by non-denaturing PAGE followed by staining for enzyme activity, revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differently affected by CdCl(2) treatment and one GR isoenzyme was shown to specifically respond to CdCl(2). The results suggest that the higher concentrations of CdCl(2) may lead to oxidative stress. The main response appears to be via the induction of SOD and CAT activities for the removal of reactive oxygen species (ROS), and by the induction of GR to ensure the availability of reduced glutathione for the synthesis of Cd-binding peptides, which may also be related to the inhibition of APX activity probably due to glutathione and ascorbate depletion.     

Evaluating the Role of Plantations as Carbon Sinks: An Example of an Integrative Approach from the Humid Tropics

/ Despite their fast growth, tropical plantations are a small sink of atmospheric carbon because they occupy only a small area in relation to other land uses worldwide. Proper design and management of plantations can increase biomass accumulation rates, making them more effective C sinks. However, fast-growing plantations can extract large amounts of nutrients from the soil, and site fertility declines may limit sustained plantation forestry after a few rotations. We measured aboveground biomass accumulation, carbon sequestration, and soil chemistry in three young plantations of 12 indigenous tree species in pure and mixed designs in the humid lowlands of Costa Rica. Annual biomass increments for the three mixed plantations ranged from 10-13 Mg/ha. The mixtures of four species gave higher biomass per hectare than that obtained by the sum of one fourth hectare of each species in pure plots. At this early age of the plantations, estimated annual C sequestration values were comparable to other reports from young plantations of exotic species commonly grown in the tropics. Four years after planting, decreases in soil nutrients were apparent in pure plots of some of the fastest growing species, while beneficial effects on soils were noted under other species. The mixed plots showed intermediate values for the nutrients examined and, sometimes, improved soil conditions. A mixture of fast and slower growing species yields products at different times, with the slower growing species constituting a longer term sink for fixed carbon. Examination of the role of tropical plantations as C sinks necessitates integrative approaches that consider rates of C sequestration, potential deleterious effects on ecosystem nutrients, and economic, social, and environmental constraints.KEY WORDS: Native trees; Aboveground biomass; Stem increments; Rotation length; Soil nutrients; Economics     

Non-homogeneous Poisson models with a change-point: an application to ozone peaks in Mexico city

In this paper, we use some non-homogeneous Poisson models in order to study the behavior of ozone measurements in Mexico City. We assume that the number of ozone peaks follows a non-homogeneous Poisson process. We consider four types of rate function for the Poisson process: power law, Musa–Okumoto, Goel–Okumoto, and a generalized Goel–Okumoto rate function. We also assume that a change-point may or may not be present. The analysis of the problem is performed by using a Bayesian approach via Markov chain Monte Carlo methods. The best model is chosen using the DIC criterion as well as graphical approach.     

Diel periodicity of pheromone release by females of Planococcus citri and Planococcus ficus and the temporal flight activity of their conspecific males

The diel periodicity of sex pheromone release was monitored in two mealybug species, Planococcus citri and Planococcus ficus (Hemiptera; Pseudococcidae), using sequential SPME/GCMS analysis. A maximal release of 2 ng/h pheromone by 9–12-day-old P. citri females occurred 1–2 h before the beginning of photophase. The highest release of pheromone by P. ficus females was 1–2 ng/2 h of 10–20-day-old females, approximately 2 h after the beginning of photophase. Mating resulted in termination of the pheromone release in both mealybug species. The temporal flight activity of the males was monitored in rearing chambers using pheromone baited delta traps. Males of both P. citri and P. ficus displayed the same flight pattern and began flying at 06:00 hours when the light was turned on, reaching a peak during the first and second hour of the photophase. Our results suggest that other biparental mealybug species display also diel periodicities of maximal pheromone release and response. Direct evaluation of the diel periodicity of the pheromone release by the automatic sequential analysis is convenient and will be very helpful in optimizing the airborne collection and identification of other unknown mealybug pheromones and to study the calling behavior of females. Considering this behavior pattern may help to develop more effective pheromone-based management strategies against mealybugs.     

Accumulation fatty acids of in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase,temperature, and co-immobilization with Azospirillum brasilense

The relation between fatty acid accumulation, activity of acetyl-CoA carboxylase (ACC), and consequently lipid accumulation was studied in the microalgae Chlorella vulgaris co-immobilized with the plant growth-promoting bacterium Azospirillum brasilense under dark heterotrophic conditions with Na acetate as a carbon source. In C. vulgaris immobilized alone, cultivation experiments for 6 days showed that ACC activity is directly related to fatty acid accumulation, especially in the last 3 days. In co-immobilization experiments, A. brasilense exerted a significant positive effect over ACC activity, increased the quantity in all nine main fatty acids, increased total lipid accumulation in C. vulgaris, and mitigated negative effects of nonoptimal temperature for growth. No correlation between ACC activity and lipid accumulation in the cells was established for three different temperatures. This study demonstrated that the interaction between A. brasilense and C. vulgaris has a significant effect on fatty acid and lipid accumulation in the microalgae.     

Carnivorans at the Great American Biotic Interchange: new discoveries from the northern neotropics

We report two fossil procyonids, Cyonasua sp. and Chapalmalania sp., from the late Pliocene of Venezuela (Vergel Member, San Gregorio Formation) and Colombia (Ware Formation), respectively. The occurrence of these pre-Holocene procyonids outside Argentina and in the north of South America provides further information about the Great American Biotic Interchange (GABI). The new specimens are recognized in the same monophyletic group as procyonids found in the southern part of the continent, the “Cyonasua group,” formed by species of Cyonasua and Chapalmalania. The phylogenetic analysis that includes the two new findings support the view that procyonids dispersed from North America in two separate events (initially, previous to the first major migration wave—GABI 1—and then within the last major migration wave—GABI 4—). This involved reciprocal lineage migrations from North to South America, and included the evolution of South American endemic forms.     

MgO-based adsorbents for CO2 adsorption: Influence of structural and textural properties on the CO2 adsorption performance

A series of MgO-based adsorbents were prepared through solution–combustion synthesis and ball-milling process.The prepared MgO-based powders were characterized using X-ray diffraction,scanning electron microscopy,N_2 physisorption measurements,and employed as potential adsorbents for CO_2 adsorption.The influence of structural and textural properties of these adsorbents over the CO_2 adsorption behaviour was also investigated.The results showed that MgO-based products prepared by solution–combustion and ball-milling processes,were highly porous,fluffy,nanocrystalline structures in nature,which are unique physico-chemical properties that significantly contribute to enhance their CO_2 adsorption.It was found that the MgO synthesized by solution combustion process,using a molar ratio of urea to magnesium nitrate(2:1),and treated by ball-milling during 2.5 hr(MgO-BM2.5h),exhibited the maximum CO_2 adsorption capacity of 1.611 mmol/g at 25℃ and 1 atm,mainly via chemisorption.The CO_2 adsorption behaviour on the MgO-based adsorbents was correlated to their improved specific surface area,total pore volume,pore size distribution and crystallinity.The reusability of synthesized MgO-BM2.5h was confirmed by five consecutive CO_2adsorption–desorption times,without any significant loss of performance,that supports the potential of MgO-based adsorbent.The results confirmed that the special features of MgO prepared by solution–combustion and treated by ball-milling during 2.5 hr are favorable to be used as effective MgO-based adsorbent in post-combustion CO_2 capture technologies.     

More than euglossines: the diverse pollinators and floral scents of Zygopetalinae orchids

Floral volatile organic compounds (VOCs) play important roles in plant-pollinator interactions. We investigated the reproductive ecology and floral VOCs of Zygopetalinae orchids to understand the relationship between floral scents and pollinators. We performed focal observations, phenological censuses and breeding system experiments in eight species in southeast Brazil. Floral scents were collected and analysed using SPME/GC-MS. We performed multivariate analyses to group species according to affinities of their VOCs and define compounds associated to each plant. Dichaea cogniauxiana was pollinated by weevils which use their developing ovules, while D. pendula was pollinated by the same weevils and perfume-collecting male euglossine bees. The other species were deceit-pollinated by bees. Zygopetalum crinitum was pollinated by carpenter bees, while W. warreana, Z. mackayi and Z. maxillare were bumblebee-pollinated. The latter was also pollinated by Centris confusa. Breeding system varied widely with no association to any pollinator group. Most VOCs are common to other floral scents. Zygopetalum crinitum presented an exclusive blend of VOCs, mainly composed of benzenoids. The scents of Pabstia jugosa, Promenaea xanthina and the Zygopetalum spp. were similar. The bumblebee-pollinated species have flowering periods partially overlapped, thus neither phenology nor pollinators constitute hybridization barriers among these species. Euglossines are not the only pollinators of Zygopetalinae. Different VOCs, size and lifespan of flowers are associated with distinct pollinators. A distinctive VOC bouquet may determine specialisation in carpenter bees or male euglossines within bee-pollinated flowers. Finally, visitation of deceit-pollinated flowers by perfume-collecting euglossines allows us to hypothesise how pollination by this group of bees had evolved.