mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei)

Mitochondrial DNA (mtDNA) has been used extensively in animals for phylogenetic and population studies but it has been applied only occasionally to mating systems analysis, where nuclear markers are usually preferred. We used denaturing-high performance liquid chromatography (D-HPLC) to detect mitochondrial DNA polymorphisms to assess the genetic mating system in Syngnathus abaster. Our study of ten pregnant males, revealed polygyny in 30% of the males, who carried embryos originating from multiple females. In addition, 30% of the pregnant males carried embryos with the same mitochondrial haplotype. This is not sufficient to demonstrate polyandry but allows a rapid selection and a reduced sample size for further studies. In conclusion, the proposed technique is time- and cost-effective, allows the certain identification of polygyny and provides useful information to study polyandry.     

Tropical fishes in a temperate sea: evolution of the wrasse <Emphasis Type="Italic">Thalassoma pavo</Emphasis> and the parrotfish <Emphasis Type="Italic">Sparisoma cretense</Emphasis> in the Mediterranean and the adjacent Macaronesian and Cape Verde Archipelagos

The northeastern Atlantic and the Mediterranean Sea share geological histories and display great faunal affinities. The majority of the Mediterranean species have Atlantic origins, with a few species with tropical affinities. These include the parrotfish Sparisoma cretense and the wrasse Thalassoma pavo that are restricted to the subtropical northeastern Atlantic, the Macaronesian archipelagos (Azores, Madeira, and Canaries) and the southern Mediterranean. The Pleistocene glaciations have been described as having different effects on the fauna of the two regions. During glacial peaks, Mediterranean waters remained warmer than those of the adjacent Atlantic. Within the eastern Atlantic, the effects of Pleistocene glaciations were differentiated. Here, we perform a comparative analysis focusing on T. pavo and S. cretense populations from the northeastern Atlantic and the Mediterranean to assess the effects of Pleistocene glaciations in these two species. Sequences from the mitochondrial control region were obtained and analyzed combining phylogeographic and demographic approaches. Gene flow between Atlantic and Mediterranean populations was shown to be very high. The Mediterranean populations of T. pavo and S. cretense showed high levels of genetic diversity, even in the eastern basin, pointing to an ancient colonization event. This suggests that both species must have been able to persist in the Mediterranean during the cold Pleistocene periods. Historical migration estimates revealed a Mediterranean towards Atlantic trend in the case of T. pavo, which may reflect the re-colonization of areas in the Atlantic by fish that survived the cold phases in relatively warmer Mediterranean refugia. Our data also showed that within the Macaronesian Archipelagos, migrations occurred from Madeira towards the Azores, for both T. pavo and S. cretense, thus supporting a post-glacial colonization of the Azores by fish that persisted in the warmer region of Madeira. Similar geographic distributions, thermal affinities, and means of dispersion for T. pavo and S. cretense resulted in a similar response to the effects of Pleistocene glaciations, as evidenced by identical phylogeographic patterns.     

Structuring pelagic trophic networks from the biomass size spectra

The selection and establishment of the structure (number and compartments, aggregation criteria, and trophic links) of the food webs is a critical task in trophic modelling. The present work proposes a systematic method to structure trophic networks in pelagic food webs. The biomass-size spectrum (BSS) is a well-established approach to analyze the structure of pelagic communities, and the body size is especially related to the ecological role of the organisms in the pelagic environment. To structure food webs, this work uses detailed arrangements of the community in size classes with increasing widths (like Sheldon-type BSS) as first aggregation criteria, and BSS theory as a framework to integrate the available knowledge about feeding selectivity in order to obtain a method to identify the trophic links between compartments. Diet composition matrices were estimated through the combination of a probability of encounter for each food type and a specific probability of ingestion related to the food size selectivity and other food quality characteristics (e.g., morphology and nutritional quality). The feasibility of this approach has been illustrated through data of size-structured communities extracted from the literature including different planktonic predator guilds (nanoflagellates, cladoceran-dominated zooplankton and copepod-dominated zooplankton) in a high mountain lake (La Caldera, Spain), two subtropical wetland lakes (meso-oligotrophic Laguna Galarza and eutrophic Laguna Iberá, Argentina) and a marine microcosm (Alborán Sea, Mediterranean). The identification of “who eats whom” and “by how much” also allows for more accurate analyses of the trophic control in the BSS. Extensive analyses of the balance between top-down and bottom-up controls were developed for the feeding interactions of the study cases.     

Environmental remediation processes by zero valence copper: reaction mechanisms

Environmental Science and Pollution Research - Recent studies have shown Cu(0) as a promising material for the removal of organic and inorganic pollutants. However, there is no review addressing...     

Controlling risks of P water pollution by sorption on soils,pyritic material,granitic material,and different by-products: effects of pH and incubation time

Environmental Science and Pollution Research - Batch experiments were used to test P sorbent potential of soil samples, pyritic and granitic materials, mussel shell, mussel shell ash, sawdust, and...     

Arsenic release from pyrite ash waste over an active hydrogeological system and its effects on water quality

Environmental Science and Pollution Research - Arsenic is a potentially toxic element of concern for environmental compartments, and it is a frequent pollutant in many abandoned industrial sites....     

A critical geography approach to land and water use in the tourism economy in Los Cabos,Baja California Sur,Mexico

ABSTRACT

This paper offers a critical assessment of how tourism development in the municipality of Los Cabos, Baja California Sur affects land and water use. Los Cabos is a seaside tourism Mecca located at the southern tip of the Baja California peninsula, in Mexico’s northwest. There, subsidised profits and a process of dispossession in the tourism sector have to do mainly with land and water appropriation in the form of restrictions to beach access by the local population, urban segregation and, in an arid region, privileging water provision for the tourism industry over the needs of the population. In order to sustain these claims, we map water and land use for tourism in Cabo San Lucas. We also analyse the expansion of the Los Cabos type of tourism to the small town of La Ribera, in the East Cape-Cabo Pulmo area, where marinas and high scale housing for residential tourism sprout along the coast. We argue that the seemingly ever-growing presence of tourism in the region carries with it an urgent call for the reappraisal of current approaches to land and water use, if local resources are to deliver quality of life for the population, and not only economic profitability for (mostly) foreign capital. Analytically, we address these issues by unpacking David Harvey’s concept of accumulation by dispossession, which – we argue – is for our purposes a broader tent than the (mostly Latin American) theorisations on extractivism, as we explain later in the relevant section of the paper.     

Toxicity evaluation of cypermethrin,glyphosate, and malathion,on two indigenous zooplanktonic species

In Aguascalientes, Mexico, there is a special concern about pesticides because of their intensive use on guava production areas, which are located in the vicinity of water reservoirs; thus, non-target organisms could be exposed. Thereafter, the aim of this work was to assess the effect of cypermethrin, Faena® (glyphosate), and malathion, which are the most used pesticides in Aguascalientes’ guava production, on the indigenous freshwater species Alona guttata (cladoceran) and Lecane papuana (rotifer). Acute 48-h toxicity tests were carried out, and LC₅₀ values were calculated. Then, five sublethal concentrations (1/80, 1/40, 1/20, 1/10, and 1/5 of the respective LC₅₀) were selected for the chronic assays: (a) intrinsic growth rate analysis in the rotifer and (b) partial life table analysis in the cladoceran. The results of the acute toxicity tests showed that A. guttata was more sensitive to malathion (LC₅₀ = 5.26 × 10^?3 mg/L) at concentrations found in natural environments with continuous application on guava fields, whereas L. papuana was more sensitive to Faena® (LC₅₀ = 19.89 mg/L). The somatic growth of A. guttata was inhibited for the chronic exposure to cypermethrin. In addition, cypermethrin and Faena® seemed to exert endocrine disruptive effects on A. guttata. Moreover, malathion chronic exposure significantly decreased the survival of A. guttata. Moreover, L. papuana was affected chronically for the three pesticides.     

Performance of an integrated system combining microalgae and vertical flow constructed wetlands for urban wastewater treatment

Environmental Science and Pollution Research - The present study investigated the performance of an integrated system, combining the sequential use of microalgae (MA) and vertical flow constructed...     

Modeling road building,deforestation and carbon emissions due deforestation in the Ecuadorian Amazon: the potential impact of oil frontier growth

In the Ecuadorian Amazon roads play the major role in transforming land cover. Since the beginning of the oil development in this region, oil exploration and road building have been linked. The objectives of this paper are twofold: First, to present a scenario of future deforestation as result of expanding the oil frontier in the Ecuadorian Amazon until the year 2030. Second, to produce an analysis of possible carbon emission scenarios as results of the deforestation produced. This paper tries to shed light into how the development of new oil concessions impact land associated environmental services. The paper uses spatial explicit simulations, that are based on parameters built using past land transitions from areas that experimented oil development, and that explain possible outcomes in the year 2030 using current and predicted road network. Simulations indicate that under current transition probabilities and a conservative scenario road construction, more than 2 million hectares will be affected, which is 35% of the area covered by concessions, which would release 51 to 148 MT of carbon to the atmosphere. This paper illustrates the strong links between road building and deforestation, where even small amount of road construction can have large effects on land cover.