Prenatal interphase FISH diagnosis of PLP1 duplication associated with Pelizaeus–Merzbacher disease

A submicroscopic genomic duplication in Xq22.2 that contains the entire proteolipid protein 1 gene (PLP1) is responsible for the majority of Pelizaeus–Merzbacher disease (PMD) patients. We previously developed an interphase FISH assay to screen for PLP1 duplications in PMD patients using peripheral blood and lymphoblastoid cell lines. This assay has been utilized as a clinical diagnostic test in our cytogenetics laboratory. To expand usage of the interphase FISH assay to prenatal diagnosis of PLP1 duplications, we examined three PMD families with PLP1 duplications utilizing aminiotic fluid samples. In two families the FISH assay revealed fetuses with PLP1 duplications, whereas the other fetus showed a normal copy number of PLP1. Haplotype analyses, as well as an additional FISH analysis using postnatal blood samples, confirmed the results of the prenatal analyses. Our study demonstrates utility of the interphase FISH assay in the prenatal diagnosis of PLP1 duplications in PMD. Copyright © 2001 John Wiley & Sons, Ltd.     

Longevity can buffer plant and animal populations against changing climatic variability

Both means and year-to-year variances of climate variables such as temperature and precipitation are predicted to change. However, the potential impact of changing climatic variability on the fate of populations has been largely unexamined. We analyzed multiyear demographic data for 36 plant and animal species with a broad range of life histories and types of environment to ask how sensitive their long-term stochastic population growth rates are likely to be to changes in the means and standard deviations of vital rates (survival, reproduction, growth) in response to changing climate. We quantified responsiveness using elasticities of the long-term population growth rate predicted by stochastic projection matrix models. Short-lived species (insects and annual plants and algae) are predicted to be more strongly (and negatively) affected by increasing vital rate variability relative to longer-lived species (perennial plants, birds, ungulates). Taxonomic affiliation has little power to explain sensitivity to increasing variability once longevity has been taken into account. Our results highlight the potential vulnerability of short-lived species to an increasingly variable climate, but also suggest that problems associated with short-lived undesirable species (agricultural pests, disease vectors, invasive weedy plants) may be exacerbated in regions where climate variability decreases.     

Resource identity modifies the influence of predation risk on ecosystem function

It is well established that predators can scare as well as consume their prey. In many systems, the fear of being eaten causes trait-mediated cascades whose strength can rival or exceed that of more widely recognized density-mediated cascades transmitted by predators that consume their prey. Despite this progress it is only beginning to be understood how the influence of predation risk is shaped by environmental context and whether it can exert an important influence on ecosystem-level processes. This study used a factorial mesocosm experiment that manipulated basal-resource identity (either barnacles, Semibalanus balanoides, or mussels, Mytilus edulis) to determine how resources modify the influence of predation risk, cascade strength, and the efficiency of energy transfer in two, tritrophic, rocky-shore food chains containing the predatory green crab (Carcinus maenas) and an intermediate consumer (the snail, Nucella lapillus). The effect of predation risk and the strength of trait-mediated cascades (both in absolute and relative terms) were much stronger in the barnacle than in the mussel food chain. Moreover, predation risk strongly diminished the efficiency of energy transfer in the barnacle food chain but had no significant effect in the mussel food chain. The influence of resource identity on indirect-effect strength and energy transfer was likely caused by differences in how each resource shapes the degree of risk perceived by prey. We suggest that our understanding of the connection between trophic dynamics and ecosystem functioning will improve considerably once the effects of predation risk on individual behavior and physiology are considered.     

Urban land use predicts West Nile virus exposure in songbirds

Urbanization is a widespread phenomenon that is likely to influence the prevalence and impact of wildlife pathogens, with implications for wildlife management and public health policies toward zoonotic pathogens. In this study, wild songbird populations were sampled at 14 sites along an urban rural gradient in the greater metropolitan Atlanta (Georgia, USA) area and tested for antibodies to West Nile virus (WNV). The level of urbanization among sites was quantitatively assessed using a principal component analysis of key land use characteristics. In total, 499 individual birds were tested during the spring and summer over three years (2004-2006). Antibody prevalence of WNV increased from rural to urban sites, and this trend was stronger among adult birds relative to juveniles. Furthermore, antibody prevalence among Northern Cardinals (Cardinalis cardinalis) was significantly higher than in other songbird species along the urban gradient. Findings reported here indicate that ecological factors associated with urbanization can influence infection patterns of this vector-borne viral disease, with likely mechanisms including changes in host species diversity and the tolerance or recovery of infected animals.     

Integrating archaeology and ancient DNA analysis to address invasive species colonization in the Gulf of Alaska

The intentional and unintentional movement of plants and animals by humans has transformed ecosystems and landscapes globally. Assessing when and how a species was introduced are central to managing these transformed landscapes, particularly in island environments. In the Gulf of Alaska, there is considerable interest in the history of mammal introductions and rehabilitating Gulf of Alaska island environments by eradicating mammals classified as invasive species. The Arctic ground squirrel (Urocitellus parryii) is of concern because it affects vegetation and seabirds on Gulf of Alaska islands. This animal is assumed to have been introduced by historic settlers; however, ground squirrel remains in the prehistoric archaeological record of Chirikof Island, Alaska, challenge this timeline and suggest they colonized the islands long ago. We used 3 lines of evidence to address this problem: direct radiocarbon dating of archaeological squirrel remains; evidence of prehistoric human use of squirrels; and ancient DNA analysis of dated squirrel remains. Chirikof squirrels dated to at least 2000 years ago, and cut marks on squirrel bones suggested prehistoric use by people. Ancient squirrels also shared a mitochondrial haplotype with modern Chirikof squirrels. These results suggest that squirrels have been on Chirikof longer than previously assumed and that the current population of squirrels is closely related to the ancient population. Thus, it appears ground squirrels are not a recent, human‐mediated introduction and may have colonized the island via a natural dispersal event or an ancient human translocation.     

Near-miss events,risk messages,and decision making

Decades of research have sought to understand how disaster preparedness decisions are made. We believe one understudied factor is the impact of near-miss events. A near-miss occurs when an event (such as a hurricane or terrorist attack) has some non-trivial probability of ending in disaster (loss of life, property damage), but the negative outcome is avoided largely by chance (e.g., at the last minute, the storm dissipates or the bomb fails to detonate). In the first of two experiments, we study reactions to a hurricane threat when participants are told about prior near-miss events. We find that people with information about a prior near-miss event that has no negative consequences are less likely to take protective measures than those with either no information or information about a prior near-miss event that has salient negative information. Similar results have been shown in prior research, but we seek to understand people’s reasoning for the different reactions. We examine the role of an individual’s risk propensity and general level of optimism as possible explanatory variables for the “near-miss” effect. We find risk propensity to be stable across conditions, whereas general optimism is influenced by the type of prior near-miss information, so that optimism mediates how near-miss information impacts protective decisions. People who experience a potentially hazardous near-miss but escape without obvious cues of damage will feel more optimistic and take less protective action. In the second study, we test messages about the hazard’s risk and examine the impact of these messages to offset the influence of near-misses. We end by discussing the implications of near-misses for risk communication.     

Understanding and managing the interactions of impacts from nature-based recreation and climate change

Disturbance to ecosystems in parks and protected areas from nature-based tourism and recreation is increasing in scale and severity, as are the impacts of climate change—but there is limited research examining the degree to which these anthropogenic disturbances interact. In this perspective paper, we draw on the available literature to expose complex recreation and climate interactions that may alter ecosystems of high conservation value such that important species and processes no longer persist. Our emphasis is on ecosystems in high demand for tourism and recreation that also are increasingly experiencing stress from climate change. We discuss the importance of developing predictive models of direct and indirect effects, including threshold and legacy effects at different levels of biological organization. We present a conceptual model of these interactions to initiate a dialog among researchers and managers so that new research approaches and managerial frameworks are advanced to address this emerging issue.     

A review of the impacts of nature based recreation on birds

Nature based recreation such as wildlife viewing, hiking, running, cycling, canoeing, horse riding and dog walking can have negative environmental effects. A review of the recreation ecology literature published in English language academic journals identified 69 papers from 1978 to 2010 that examined the effect of these activities on birds. Sixty-one of the papers (88%) found negative impacts, including changes in bird physiology (all 11 papers), immediate behaviour (37 out of 41 papers), as well as changes in abundance (28 out of 33 papers) and reproductive success (28 out of 33 papers). Previous studies are concentrated in a few countries (United States, England, Argentina and New Zealand), mostly in cool temperate or temperate climatic zones, often in shoreline or wetland habitats, and mostly on insectivore, carnivore and crustaceovore/molluscivore foraging guilds. There is limited research in some regions with both high bird diversity and nature based recreation such as mainland Australia, Central America, Asia, and Africa, and for popular activities such as mountain bike riding and horse riding. It is clear, however, that non-motorised nature based recreation has negative impacts on a diversity of birds from a range of habitats in different climatic zones and regions of the world.