Classification of coastal waters according to the new Italian water legislation and comparison with the European Water Directive

New legislation for the protection of inland surface waters, transitional waters, coastal waters and ground waters has recently been established in Italy. This law presents a new integrated approach, where all water bodies are considered as complex ecosystems to be studied in each of their components. The new concept of environmental quality of the water body, based on the ecological and chemical status, is also introduced. At the same time (i.e. the end of 2000), the European Community approved the European Water Framework Directive based on the same basic environmental concepts and criteria of the new Italian law. This paper analyses the important points and innovations required by the new Italian legislation for monitoring and classification of marine coastal waters. Details of definitions, parameters, analysis and monitoring programs are discussed. A comparison with the European Water Framework Directive is eventually given, underlining the specific characteristics of the Mediterranean sea, which have to be taken into consideration when applying the European Directive to this particular ecoregion.     

Geographical Range Size and the Conservation of Mexican Mammals

Range was estimated for the 423 noninsular mammals of Mexico to identify those species with more restricted distributions and to detect priority areas for conservation based on the presence of such species. Thirty-eight percent of nonvolant mammals and 15.4% of bats are restricted in Mexico to areas of less than 114,000 km². Restricted species were defined as those occurring in ranges smaller than the median for bats and for nonvolant species. Following this criterion, most nonvolant species with restricted distribution in Mexico are either endemic to the country or are shared with the United States, whereas endemic chiropteran species are few, and most Mexican bats with restricted distribution also occur in South America. Nonvolant mammals with restricted distribution in Mexico tend to be of small body size, herbivore or granivore, and fossorial or semifossorial. Among bats, gleaners are significantly more restricted than aerial insectivores. Species with restricted distribution are inadequately represented in the current official list of endangered species, particularly in the case of nonvolant mammals. Similarly, some areas of Mexico that harbor several species with restricted distribution are not represented in the Mexican system of protected areas. Therefore, rarity, in this case measured by the area of distribution, should be included as an additional criterion for conservation of the Mexican mammal fauna.     

Occurrence of <Emphasis Type="Italic">Pepper Mild Mottle Virus</Emphasis> (PMMoV) in Groundwater from a Karst Aquifer System in the Yucatan Peninsula,Mexico

The Yucatan Peninsula of Mexico hosts a karst aquifer system that is the only source of freshwater for the area; however, it is vulnerable to human-mediated contamination. Pepper mild mottle virus (PMMoV) is one of the most abundant RNA viruses associated with human feces, making it a viable indicator for tracking fecal pollution in aquatic environments, including groundwater. In this study, groundwater samples collected from a karst aquifer from fresh and brackish water locations were analyzed for fecal indicator bacteria, somatic and male F+ specific coliphages, and PMMoV during the rainy and dry seasons. Total coliform bacteria were detected at all sites, whereas Escherichia coli were found at relatively low levels <40 MPN/100 ml. The highest average concentrations of somatic and male F+ specific coliphages were 920 and 330 plaque forming units per 100 ml, respectively, detected in freshwater during the rainy season. PMMoV RNA was detected in 85% of the samples with gene sequences sharing 99–100% of nucleotide identity with PMMoV sequences available in GenBank. Quantification of PMMoV genome copies (GC) by quantitative real-time PCR indicated concentrations ranging from 1.7 × 10¹ to 1.0 × 10⁴ GC/L, with the highest number of GC detected during the rainy season. No significant correlation was observed between PMMoV occurrence by season or water type (p > 0.05). Physicochemical and indicator bacteria were not correlated with PMMoV concentrations. The abundance and prevalence of PMMoV in the karst aquifer may reflect its environmental persistence and its potential as a fecal indicator in this karst aquifer system.     

Beyond opportunity costs: who bears the implementation costs of reducing emissions from deforestation and degradation?

Reducing Emissions from Deforestation and Degradation (REDD+) in developing countries is based on the premise that conserving tropical forests is a cost-effective way to reduce carbon emissions and therefore can be fully funded by international actors with obligations or interests in reducing emissions. However, concerns have repeatedly been raised about whether stakeholders in REDD+ host countries will actually end up bearing the costs of REDD+. Most prior analyses of the costs of REDD+ have focused on the opportunity costs of foregone alternative uses of forest land. We draw on a pan-tropical study of 22 subnational REDD+ initiatives in five countries to explore patterns in implementation costs, including which types of organizations are involved and which are sharing the costs of implementing REDD+. We find that many organizations involved in the implementation of REDD+, particularly at the subnational level and in the public sector, are bearing implementation costs not covered by the budgets of the REDD+ initiatives. To sustain this level of cost-sharing, REDD+ must be designed to deliver local as well as global forest benefits.     

Environmental Surveillance of SARS-CoV-2 RNA in Wastewater and Groundwater in Quintana Roo,Mexico

The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater has been reported as a result of fecal shedding of infected individuals. In this study, the occurrence of SARS-CoV-2 RNA was explored in primary-treated wastewater from two municipal wastewater treatment plants in Quintana Roo, Mexico, along with groundwater from sinkholes, a household well, and submarine groundwater discharges. Physicochemical variables were obtained in situ, and coliphage densities were determined. Three virus concentration methods based on adsorption-elution and sequential filtration were used followed by RNA isolation. Quantification of SARS-CoV-2 was done by RT-qPCR using the CDC 2020 assay, 2019-nCoV_N1 and 2019-nCoV_N2. The Pepper mild mottle virus, one of the most abundant RNA viruses in wastewater was quantified by RT-qPCR and compared to SARS-CoV-2 concentrations. The use of three combined virus concentration methods together with two qPCR assays allowed the detection of SARS-CoV-2 RNA in 58% of the wastewater samples analyzed, whereas none of the groundwater samples were positive for SARS-CoV-2 RNA. Concentrations of SARS-CoV-2 in wastewater were from 1.8 × 10³ to 7.5 × 10³ genome copies per liter (GC l⁻¹), using the N1 RT-qPCR assay, and from 2.4 × 10² to 5.9 × 10³ GC l⁻¹ using the N2 RT-qPCR assay. Based on PMMoV prevalence detected in all wastewater and groundwater samples tested, the three viral concentration methods used could be successfully applied for SARS-CoV-2 RNA detection in further studies. This study represents the first detection of SARS-CoV-2 RNA in wastewater in southeast Mexico and provides a baseline for developing a wastewater-based epidemiology approach in the area.

     

Characterization of municipal waste in Kampala,Uganda

In Kampala, Uganda, about 28,000 tons of waste is collected and delivered to a landfill every month. Kampala Capital City Authority (KCCA) records show that this represents approximately 40% of the waste generated in the city. The remaining uncollected waste is normally dumped in unauthorized sites, causing health and environmental problems. However, the organic fraction of domestic waste can provide an opportunity to improve livelihoods and incomes through fertilizer and energy production. This study characterized the municipal waste generated in Kampala and delivered to Kiteezi landfill between July 2011 and June 2012, that is, covering the dry and wet months. On each sampling day, waste was randomly selected from five trucks, sorted and weighed into different physical fractions. Samples of the organic waste from each truck were analyzed for total solids, major nutrients, and energy content. During the wet months, the waste consisted of 88.5% organics, 3.8% soft plastics, 2.8% hard plastics, 2.2% paper, 0.9% glass, 0.7% textiles and leather, 0.2% metals, and 1.0% others. During the dry months, the waste consisted of 94.8% organics, 2.4% soft plastics, 1.0% hard plastics, 0.7% papers, 0.3% glass, 0.3% textile and leather, 0.1% metals, and 0.3% others. The organic waste on average had a moisture content of 71.1% and contained 1.89% nitrogen, 0.27% phosphorus, and 1.95% potassium. The waste had an average gross energy content of 17.3 MJ/kg. It was concluded that the organic waste generated can be a suitable source of some plant nutrients that are useful especially in urban agriculture.

Implications:?The result of the waste characterization in Kampala was found to be significantly different from that obtained for other Sub-Saharan African (SSA) cities, showing that studies assuming average values for the waste fractions are likely to result in erroneous results. Furthermore, no reduction in organic fraction of the waste was noticed when compared with a study done two decades ago in spite of greatly improved economic status of Kampala city, a finding that is not in agreement with several other similar studies done for other SSA cities.     

Dissipation of 2,4-D in soils of the Humid Pampa region, Argentina: a microcosm study

Phenoxy herbicides like 2,4-dichlorophenoxyacetic acid (2,4-D) are widely used in agricultural practices. Although its half life in soil is 7-14d, the herbicide itself and its first metabolite 2,4-dichlorophenol (2,4-DCP) could remain in the soil for longer periods, as a consequence of its intensive use. Microcosms assays were conducted to study the influence of indigenous microflora and plants (alfalfa) on the dissipation of 2,4-D from soils of the Humid Pampa region, Argentina, with previous history of phenoxy herbicides application. Results showed that 2,4-D was rapidly degraded, and the permanence of 2,4-DCP in soil depended on the presence of plants and soil microorganisms. Regarding soil microbial community, the presence of 2,4-D degrading bacteria was detected even in basal conditions in this soil, possibly due to the adaptation of the microflora to the herbicide. There was an increment of two orders of magnitude in herbicide degraders after 15d from 2,4-D addition, both in planted and unplanted microcosms. Total heterotrophic bacteria numbers were about 1x10(8) CFUg(-1) dry soil and no significant differences were found between different treatments. Overall, the information provided by this work indicates that the soil under study has an important intrinsic degradation capacity, given by a microbial community adapted to the presence of phenoxy herbicides.