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81.
Dinitrotoluenes are widely used as solvents and are intermediates in the synthesis of dyes, explosives, and pesticides. Environmental concerns regarding DNTs have increased due to their widespread use and their discharge into the environment. In this study, the anaerobic biodegradation of four dinitrotoluene isomers, 2,3-, 2,4-, 2,6- and 3,4-DNT, was investigated using Lactococcus lactis subsp. lactis strain 27, which was isolated from the intestines of earthworms. Liquid chromatography/mass spectrometry and NMR spectroscopy showed that L. lactis strain 27 non-specifically reduced the nitro groups on the tested dinitrotoluenes to their corresponding aminonitrotoluenes. L. lactis strain 27, however, did not reduce either sequentially or simultaneously two nitro groups of the dinitrotoluenes, resulting in the formation of the corresponding diaminotoluenes. In vitro formation of dinitroazoxytoluenes suggested the presence of oxygen-sensitive hydroxylaminonitrotoluenes. L. lactis strain 27 was capable of reducing 2,4-, 2,6-, 2,3-, and 3,4-dinitrotoluenes up to 173.6, 66.6, 287.1, and 355 microM, respectively in 12 h incubation. A relatively rapid reduction was observed in the case of the 2,3-, and 3,4-dinitrotoluenes, which have vicinal nitro groups on their arene structure. Non-specific anaerobic reduction of dinitrotoluenes by the intestinal bacterium L. lactis strain 27 differentiated the extent of reduction of DNTs according to the substitutional position of the nitro groups and produced in vitro more toxic dinitroazoxytoluenes, suggesting that anaerobic biotransformation of dinitrotoluenes could increase environmental risk. 相似文献
82.
Dr. Lilian M. Lopes Sang Choon Cha Edécio Armbruster De Moraes Marcelo Zugaib 《黑龙江环境通报》1995,15(2):183-185
Fetal echocardiography was performed during the third trimester in a normal primigravida. The fetal heart was severely affected with the typical cardiac manifestations of Marfan syndrome. The medical history of the father was investigated and a mild form of the syndrome was diagnosed. The neonate died at 2 months of age of congestive heart failure. 相似文献
83.
Samomssa Inna Nono Yvette Jiokap Cârâc Geta Gurău Gheorghe Dinică Mihaela Rodica Kamga Richard 《Journal of Material Cycles and Waste Management》2021,23(5):1905-1917
Journal of Material Cycles and Waste Management - Mechanical properties enable to handle storage and transport of fuel briquette. Binder is key element of these mechanical properties. Biobinder is... 相似文献
84.
A Microsoft Excel spreadsheet‐based design tool has been developed to assist remediation professionals in the design of injection systems for distributing soluble substrate (SS) to enhance in situ anaerobic bioremediation. The user provides site data, design parameters, and unit‐cost information to generate estimates of remediation‐system cost and steady‐state contact efficiency (CESS) for various designs. CESS is estimated from a nonlinear regression equation that includes terms for the SS injection concentration (CI), minimum substrate concentration (CMIN), groundwater travel time between rows of injection wells (TT), SS half‐life (TH), substrate reinjection time interval (TR), and pore volumes of substrate solution injected (PV). With this tool, users can quickly compare the relative costs and performance of different injection alternatives and identify the best design for their specific site conditions. The design process embodied in the tool includes: (1) entering injection‐well configuration and unit costs for well installation, injection, and substrate; (2) determining treatment‐zone dimension; (3) selecting trial injection‐well spacing, time period between substrate reinjection, and injection pore volume; and (4) estimating contact efficiency and capital and life‐cycle costs. This process is then repeated until a final design is selected. In most cases, injection costs increase with increasing CESS. However, the best (highest) ratio of CESS to injection cost typically occurs for CESS in the range of 70 to 80 percent. © 2013 Wiley Periodicals, Inc. 相似文献
85.
Tânia Rodrigues Pereira Ramos Maria Isabel Gomes Ana Paula Barbosa-Póvoa 《Waste management (New York, N.Y.)》2013,33(8):1691-1703
This research has been motivated by a real-life problem of a waste cooking oil collection system characterized by the existence of multiple depots with an outsourced vehicle fleet, where the collection routes have to be plan. The routing problem addressed allows open routes between depots, i.e., all routes start at one depot but can end at the same or at a different one, depending on what minimizes the objective function considered. Such problem is referred as a Multi-Depot Vehicle Routing Problem with Mixed Closed and Open Inter-Depot Routes and is, in this paper, modeled through a mixed integer linear programming (MILP) formulation where capacity and duration constraints are taken into account. The model developed is applied to the real case study providing, as final results, the vehicle routes planning where a decrease of 13% on mileage and 11% on fleet hiring cost are achieved, when comparing with the current company solution. 相似文献
86.
87.
António Pires Lina Ramos Margarida Venâncio Ana Isabel Rei Sérgio Castedo Jorge Saraiva 《黑龙江环境通报》2005,25(4):292-295
The authors describe a case of a male foetus whose ultrasound at 20 weeks' gestation revealed cystic hygroma, cleft lip and ventricular septal defect. Amniotic fluid cytogenetics using GTG banding showed a 46,XY,der(13)t(3;13)(q12;p11.1) rearrangement, and fluorescence in situ hybridization (FISH) delineated the relevant breakpoints. Familial studies identified a maternal balanced translocation involving chromosomes 3 and 13. The post-mortem examination confirmed the prenatal ultrasound findings. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
88.
C. Goubert C. Josso P. Louâpre A. M. Cortesero D. Poinsot 《Die Naturwissenschaften》2013,100(2):177-184
Parasitoids of phytophagous insects face a detectability–reliability dilemma when foraging for hosts. Plant-related cues are easily detectable, but do not guarantee the presence of the host. Host-related cues are very reliable, but much harder to detect from a distance. Little is known in particular about the way coleopteran parasitoid females use these cues when foraging for a suitable place to lay their eggs. The question is of interest because, unlike hymenopteran larvae, coleopteran parasitoid larvae are highly mobile and able to forage for hosts on their own. We assessed whether females of the parasitoid rove beetle Aleochara bipustulata (L.) (Coleoptera: Staphylinidae) are attracted to plant (Swede roots, Brassica napus) and host-related cues [pupae of the cabbage root fly Delia radicum (L.) (Diptera: Anthomyiidae)]. In the field, A. bipustulata adult females were captured in selective pitfall traps containing pieces of roots damaged by D. radicum larvae, but not in traps containing pieces of healthy roots or D. radicum pupae. However, in the laboratory, the odour of D. radicum pupae attracted A. bipustulata females to mini-pitfalls. Video monitoring in the laboratory showed that foraging A. bipustulata females preferred a zone containing D. radicum pupae and larval tracks rather than one containing an extract of D. radicum-infested roots. Our results suggest a behavioural sequence where A. bipustulata females use plant-related cues at a distance, but then switch their preference to host-related cues at a close range. This would be the first observation of this behaviour in coleopteran parasitoids. 相似文献
89.
Pereira Diogo Rocha Luciana S. Gil María V. Otero Marta Silva Nuno J. O. Esteves Valdemar I. Calisto Vânia 《Environmental science and pollution research international》2021,28(15):18314-18327
Environmental Science and Pollution Research - The main goal of this work was to produce an easily recoverable waste-based magnetic activated carbon (MAC) for an efficient removal of the... 相似文献
90.
Tiago José Pereira Gustavo Fonseca Manuel Mundo-Ocampo Betânia Cristina Guilherme Axayácatl Rocha-Olivares 《Marine Biology》2010,157(8):1665-1678
We used morphological and molecular approaches to evaluate the diversity of free-living marine nematodes (order Enoplida)
at four coastal sites in the Gulf of California and three on the Pacific coast of Baja California, Mexico. We identified 22
morphological species belonging to six families, of which Thoracostomopsidae and Oncholaimidae were the most diverse. The
genus Mesacanthion (Thoracostomopsidae) was the most widespread and diverse. Five allopatric species, genetically and morphologically differentiated,
were found in two localities in the Gulf of California (M. sp1 and M. sp2) and three in the Pacific coast (M. sp3, M. sp4 and M. sp5). Overall, we produced 19 and 20 sequences for the 18S and 28S genes, respectively. Neither gene displayed intraspecific
polymorphisms, which allowed us to establish that some morphological variation was likely either ontogenetic or due to phenotypic
plasticity. Although 18S and 28S phylogenies were topologically congruent (incongruence length difference test, P > 0.05), divergences between species were much higher in the 28S gene. Moreover, this gene possessed a stronger phylogenetic
signal to resolve relationships involving Rhabdodemania and Bathylaimus. On the other hand, the close relationship of Pareurystomina (Enchilidiidae) with oncholaimids warrants further study. The 28S sequences (D2D3 domain) may be better suited for DNA barcoding
of marine nematodes than those from the 18S rDNA, particularly for differentiating closely related or cryptic species. Finally,
our results underline the relevance of adopting an integrative approach encompassing morphological and molecular analyses
to improve the assessment of marine nematode diversity and advance their taxonomy. 相似文献