Effect of Diel Activity Patterns and Harvesting Pressure on the Diversity and Biomass of Sea Cucumbers in Marovo Lagoon, Solomon Islands

A marked decline in the contribution by Marovo Lagoon to the annual total bêche-de-mer production of the Solomon Islands from 58% in 1989 to 17% in 2003 prompted investigation of their current biomass and diversity. We also assessed changes to critical ecological services and the prospects for population recovery following a fisheries closure. Day time and nocturnal transects revealed a mean abundance of 32.4 (SD = 5.3) low value species per ha (e.g. Holothuria atra, H. edulis, H. coluber and Thelenota anax) and 15.2 (SD = 2.7) high value species per ha (e.g. H. fuscogilva, Actinopyga lecanora, Stichopus hermanni and Thelenota ananas). Following a 17 month closure of the fishery (2005–2007), the abundance of bêche-de-mer was reported by local fisherman to have increased; however, no scientific studies were conducted that can substantiate this community held belief. The current study aimed to document the impact of re-opening the fishery in 2007 and documented a decline in high value species of 9% over a 5 month period following the opening of the fishery, while low value species continued to increase in abundance by 11%, over the same period based on nocturnal surveys. Continued observation of the recovery, post closure, and any subsequent harvest in Marovo will be required to properly understand population dynamics and provide a sustainable harvest plan for bêche-de-mer in the future.     

In vivo endocrine effects of naphthenic acids in fish

Oil pollution from various sources, including exploration, production and transportation, is a growing global concern. The highest toxicity of hydrocarbon pollutants is associated with the water-soluble phase compounds, including naphthenic acids, a known component found in all hydrocarbon deposits. Recently, naphthenic acids (NAs) have shown estrogenic and anti-androgenic effects in vitro. For this reason we investigated the potential effects of two commercial mixtures of naphthenic acids on fish in vivo, using the three-spined stickleback (Gasterosteus aculeatus) as a model species.     

Denaturing high-performance liquid chromatography (DHPLC)-based prenatal diagnosis for tuberous sclerosis

Tuberous sclerosis (TSC) is a frequent autosomal-dominant condition (affecting 1 in 6000 individuals) caused by various mutations in either the hamartin (TSC1) or the tuberin gene (TSC2). This allelic and non-allelic heterogeneity makes genetic counseling and prenatal diagnosis difficult, especially as a significant proportion of TSC cases are due to de novo mutations. For this reason the identification of the disease causing mutation is mandatory for accurate counseling, yet current mutation detection methods such as single-strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE) are labor intensive with limited detection efficiency. Denaturing high-performance liquid chromatography (DHPLC) is a high-throughput, semi-automated mutation detection system with a reported mutation detection rate close to 100% for PCR fragments of up to 800 bp. We used a recently described DHPLC assay allowing the efficient detection of mutations in TSC1 to analyze the DNA extracted from a chorion villus sample in order to perform a prenatal diagnosis for TSC. The fetus was found not to have inherited the deleterious mutation and the DHPLC diagnosis was confirmed by haplotype analysis. This represents the first DHPLC-based prenatal diagnosis of a genetic disease. Copyright © 2001 John Wiley & Sons, Ltd.     

Cost of parental care in the woodroach Cryptocercus punctulatus Scudder (Dictyoptera: Cryptocercidae)

Summary The objective of this study was to measure the effect of parental care on the reproductive capabilities and survival of adult Cryptocercus punctulatus Scudder. Brood removal studies show that the long term parental care exhibited by this woodroach can exact a cost in terms of future reproduction by delaying a second reproductive episode. If C. punctulatus is used as a model of the ancestral state of the termites, changes in responsibility for care giving behavior can account for all the higher components of eusociality (overlap of worker generations, brood care by workers, non-reproductive castes) by shifting the cost of parental care from the adults to the oldest offspring.     

Enantiomeric separation of metolachlor and its metabolites using LC-MS and CZE

The stereoisomers of metolachlor and its two polar metabolites [ethane sulfonic acid (ESA) and oxanilic acid (OXA)] were separated using liquid chromatography-mass spectrometry (LC-MS) and capillary zone electrophoresis (CZE), respectively. The separation of metolachlor enantiomers was achieved using a LC-MS equipped with a chiral stationary phase based on cellulose tris(3,5-dimethylphenyl carbamate) and an atmospheric pressure chemical ionization source operated under positive ion mode. The enantiomers of ESA and OXA were separated using CZE with gamma-cyclodextrin (gamma-CD) as chiral selector. Various CZE conditions were investigated to achieve the best resolution of the ESA and OXA enantiomers. The optimum background CZE electrolyte was found to consist of borate buffer (pH=9) containing 20% methanol (v/v) and 2.5% gamma-CD (w/v). Maximum resolution of ESA and OXA enantiomers was achieved using a capillary temperature of 15 degrees C and applied voltage of 30 kV. The applicability of the LC-MS and CZE methods was demonstrated successfully on the enantiomeric analysis of metolachlor and its metabolites in samples from a soil and water degradation study that was set up to probe the stereoselectivity of metolachlor biodegradation. These techniques allow the enantiomeric ratios of the target analytes to be followed over time during the degradation process and thus will prove useful in determining the role of chirality in pesticide degradation and metabolite formation.