The weaker points of fish acute toxicity tests and how tests on embryos can solve some issues

Fish acute toxicity tests play an important role in environmental risk assessment and hazard classification because they allow for first estimates of the relative toxicity of various chemicals in various species. However, such tests need to be carefully interpreted. Here we shortly summarize the main issues which are linked to the genetics and the condition of the test animals, the standardized test situations, the uncertainty about whether a given test species can be seen as representative to a given fish fauna, the often missing knowledge about possible interaction effects, especially with micropathogens, and statistical problems like small sample sizes and, in some cases, pseudoreplication. We suggest that multi-factorial embryo tests on ecologically relevant species solve many of these issues, and we shortly explain how such tests could be done to avoid the weaker points of fish acute toxicity tests.     

Cytogenetic evaluation and the association with polymorphisms of the CPY1A1 and NR1I3 genes in individuals exposed to BTEX

The gas station attendants are exposed daily to chemical agents that compose gasoline, such as BTEX (benzene, toluene, ethylbenzene, and xylene), and the exposure to these agents can cause a variety of effects on the human health. Among the various possible cell alterations associated with these exposures are the formation of micronuclei and of binucleated cells which are used as indicators of clastogenic action. Benzene, the main carcinogenic agent, is metabolized to more soluble forms and easily excreted by isoenzymes of cytochrome P450, such as CYP1A1. The CYP1A1 gene is highly polymorphic and one of its allele variations can be detected by the use of restriction endonucleasis MspI and is originated by the transition of a thymine by a cytosine (3798T>C), resulting in the polymorphic allele CYP1A1*2A. The objective of this study was to evaluate the cytogenetic damage induced by the exposure to BTEX and to associate it with the polymorphisms of the CYP1A1 and NR1I3 genes. Samples of exfoliated cells from the oral mucosa of 27 gas station attendants and from a control group were collected. The results found show that the group exposed to BTEX presents significantly higher alterations than those in the control group for micronuclei (MN; 6.85?±?1.33 vs. 2.96?±?1.91, P?<?0.001) and for the total of nuclear alterations observed (MN + binucleated cells (BNC); 9.59?±?4.73 vs. 5.07?±?2.21, P?<?0.001). When comparing the cytological alterations and the genotypes among the exposed individuals for the polymorphism 3798T>C of the CYP1A1 gene, homozygotes TT present MN + BNC significantly higher than carriers of the allele C (10.88?±?5.36 vs. 5.33?±?2.52, P?=?0.028). No association was observed in the control group or for the NR1I3 gene. These results show that molecular and cytogenetic data can be used in the future as tools to monitor individuals exposed to such compounds.     

Development and Pilot Plant Evaluation of Silica- Enhanced Lime Sorbents for Dry Flue Gas Desulfurization

EPA’s efforts to develop low cost, retrofitable flue gas cleaning technology include the development of highly reactive sorbents. Recent work addressing lime enhancement and testing at the bench-scale followed by evaluation of the more promising sorbents in a pilot plant are discussed here.

The conversion of Ca(OH)₂ with SO₂ increased several-fold compared with Ca(OH)₂ alone when Ca(OH)₂ was slurrled with fly ash first and later exposed to SO₂ in a laboratory packed bed reactor. Ca(OH)₂ enhancement increased with the increased fly ash amount. Dlatomaceous earths were very effective reactivity promoters of lime-based sorbents. Differential scanning calorimetry of the promoted sorbents revealed the formation of a new phase (calcium silicate hydrates) after hydration, which may be the basis for the observed Improved SO₂ capture.

Fly ash/lime and diatomaceous earth/lime sorbents were tested in a 100 m³/h pilot facility incorporating a gas humidifier, a sorbent duct injection system, and a baghouse. The inlet SO₂ concentration range was 1000-2500 ppm. With once-through dry sorbent injection into the humidified flue gas [approach to saturation 10–20°C (18–36°F) in the baghouse], the total SO₂ removal ranged from 50 to 90 percent for a stoichiometric ratio of 1 to 2. Recycling the collected solids resulted in a total lime utilization exceeding 80–90 percent. Increased lime utilization was also investigated by the use of additives.     

Migration and animal husbandry: Competing or complementary livelihood strategies.Evidence from Kyrgyzstan

Animal husbandry and labour migration are important livelihood strategies for a large proportion of the rural population in developing countries. Up to now, the two strategies have usually been studied by looking at either one or the other; their interlinkages have rarely been examined. Based on a case study in rural Kyrgyzstan, the aim of this paper is to explore the links between animal husbandry and labour migration. Results show that for most rural households, livestock is crucial yet not sufficient to make a living. Therefore, many people diversify their income sources by migrating to work elsewhere. This generates cash for daily expenses and the acquisition of new livestock, but also leads to an absence of workforce in households. Yet since remittances usually exceed the expenses for hiring additional workforce, most people consider migration profitable. From a socio‐economic point of view, migration and animal husbandry can thus be considered important complementary livelihood strategies for the rural Kyrgyz population, at least for the time being. In the long term, however, the failure of young migrants to return to rural places and their settlement in urban areas might also cause remittance dependency and lead to an increasing lack of qualified labour. From an environmental point of view, the investment of remittances into animal husbandry poses challenges to sustainable pasture management. Increasing livestock numbers in rural areas raise pressure on pasture resources. Since most people consider animal husbandry their main future prospect while continuing to use pastures in a fairly unsustainable way, this may further exacerbate the over‐utilization of pastures in future.     

Chemical composition of material fractions in Danish household waste

The chemical composition of Danish household waste was determined by two approaches: a direct method where the chemical composition (61 substances) of 48 material fractions was determined after hand sorting of about 20 tonnes of waste collected from 2200 households; and an indirect method where batches of 80–1200 tonnes of unsorted household waste was incinerated and the content of the waste determined from the content of the outputs from the incinerator. The indirect method is believed to better represent the small but highly contaminated material fractions (e.g., batteries) than the direct method, because of the larger quantities included and the more homogenous material to sample from. Differences between the direct and the direct methods led to corrections in the of heavy metal concentration of a few fractions.The majority of the energy content of the waste originates from organic waste like paper, cardboard and organic fractions. The single fraction contributing most to the total energy content is the non-recyclable plastic fraction, contributing 21% of the energy content and 60% of the chlorine content, although this fraction comprises less than 7% by weight. Heavy metals originate mainly from inert fractions, primarily batteries.     

Anwendung differenzierter Untersuchungsmethoden zur Sedimentbewertung

The aquatic sediments have special significance as habitats of species-rich biocoenoses and as the place where manifold transformation processes occur. Because of their high potential for accumulation of contaminants, sediments are particularly sensitive to anthropogenic impacts, which may disturb the natural state of sediments. In order to protect the aquatic life community, such impacts must be identified, assessed, and the chemical causes of toxic effects must be found. Since the end of the 1980s, the interest for a possibly comprehensive assessment of sediments has increased. This problem can be tackled only by an integrated approach, which combines the detection of toxicity under standardized laboratory conditions with chemical data and biologicalin situ studies into a holistic view. The information value of the results depends essentially on the selection of a diversity of test methods, which are able to indicate contaminant effects differentially. This paper presents results from a comprehensive test approach, which integrates standardized methods (DIN) with pore waters and eluates, as well as tests using the whole sediment. To cover a wider variety of contaminants in the sediment, solvent extracts and fractions thereof were also examined by different bioassays. Chemical analyses examined the structural parameters and identified priority contaminants. Non-target screening could detect a variety of further substances and substance classes. The model organisms of the bioassays responded very differentially and sometimes very intensively to the contaminants in the various investigation media. Especially in sediment extracts, some fractions, and consequently also substances and groups of substances, proved to be particularly toxic. It could be shown that the selected chemical, ecotoxicological, and biological study methods contribute to a holistic assessment. Further, possibly very sensitive bioassays and benthos-biological parameters should be examined aiming to optimize the very wide battery of tests.