Monitoring individual exposure. Measurements of volatile organic compounds in breathing-zone air, drinking water, and exhaled breath

Methods for determining individual exposure to volatile organic compounds (VOC) during normal daily activities were field tested on university student volunteers in Texas and North Carolina. The equipment tested included a personal monitor employing Tenax GC® to collect organic vapors for later analysis by GC-MS, and a specially designed spirometer for collecting samples of expired human breath on duplicate Tenax cartridges. The personal monitor and spirometer proved feasible for collecting abundant quantitative data on most of the 15 target organics. Air exposures to many VOC varied widely, sometimes over three orders of magnitude, among students on the same campus who had been monitored over the same time period and day. A log-linear relationship between breathing-zone air exposures and concentrations in exhaled breath was suggested for three chemicals: tetrachloroethylene, 1,1,1-trichloroethane, and vinylidene chloride. Air was the main route of exposure for all target compounds except the two trihalomethanes (chloroform and bromodichloromethane), which were transmitted mainly through water. Estimated total daily intake through air and water of the target organics ranged from 0.3 to 12.6 mg, with 1,1,1-trichloroethane at the highest concentrations in both geographic areas.     

Mitigation of chlorpyrifos runoff using constructed wetlands

Constructed wetlands have been proposed as a potential best management practice (BMP) to mitigate effects of pesticide-associated agricultural runoff. Wetland mesocosms (14 m x 59-73 m) were amended with chlorpyrifos to simulate a storm runoff event at concentrations of 73, 147 and 733 microg/l. Water, sediment and plant samples collected weekly for 12 weeks indicated that chlorpyrifos rapidly sorbed to sediment and plant material, with approximately 47-65% of measured chlorpyrifos mass retained within the first 30-36 m of wetland mesocosms. Of the measured mass, approximately 55% and 25% were retained by sediments and plants, respectively. A field-scale evaluation of a constructed wetland's mitigation capability was performed in the Lourens River watershed of Cape Town, South Africa. Results indicate that the wetland was able to retain and considerably decrease the concentration (and hence toxicity) of chlorpyrifos and suspended sediment entering the receiving waterbody (Lourens River). This research provides fundamental answers concerning constructed wetland capabilities that are necessary for constructing field-scale systems within agricultural watersheds.     

On the propagation of error in air pollution measurements

Four methods for estimating the uncertainties in air pollution measurements are outlined. The approaches are: analytical solution-approximation; application of distribution theory; experimentation; and simulation. The advantages and disadvantages of each method are illustrated using data from High-Volume air samplers, the instrument most commonly used for monitoring ambient concentrations of airborne particles.Dr Evans is Assistant Professor of Environmental Science and Mr Kinney is a doctoral candidate in the Department of Environmental Science and Physiology, Harvard School of Public Health. Dr Cooper is at the Manufacturing Research Laboratory, Thomas J. Watson Research Center, IBM, Yorktown Heights, NY.     

Patterns of Avian Nest Predators and a Brood Parasite Among Restored Riparian Habitats in Agricultural Watersheds

In fragmented edge-dominated landscapes, nest predation and brood parasitism may reduce avian reproductive success and, ultimately, populations of some passerine species. In the fragmented agroecosystem of northwest Mississippi, placement of drop-pipe structures has been used as a restoration technique for abating gully erosion along stream banks. These actions have formed small herbaceous and woody habitat extensions into former agricultural lands. We quantified species relative abundances, species richness, and evenness of avian nest predators and a brood parasite within four categories of constructed habitat resulting from drop-pipe installation. Differences in the abundance of two nest predators, cotton mouse (Peromyscus gossypinus) and blue jay (Cyanocitta cristata), were observed among constructed habitats. However, relative abundances of other predators such as common grackle (Quiscalus quiscula), American crow (Corvus brachyrhynchos), and hispid cotton rat (Sigmodon hispidus), and the obligate brood parasite brown-headed cowbird (Molothrus ater) did not differ among four habitat categories. Although species richness, abundance, and evenness of potential nest predators were generally similar among the constructed habitats, predator species composition varied, suggesting that these habitats supported different predator communities. This difference is important because as each predator species is added to or deleted from the community, variation may occur in the framework of prey search methods, predator strategies, and potentially overall predation pressure. We suggest that land managers using drop-pipes as part of stream restoration projects allow for the development of the constructed habitat with the largest area and greatest vegetative structure.     

Mercury bioaccumulation in organisms from three Puerto Rican estuaries

We analyzed mercury levels in shrimp (Palaemonetes sp.), Blue Crabs (Callinectes sp.), fish (Tarpon Megalops atlantica and Tilapia Tilapia mossambica), lizards (Ameiva exsul), Cattle Egret (Bubulcus ibis) and Moorhen (Gallinula chloropus) in three estuaries in Puerto Rico in 1988. There were no quantifiable concentrations greater than the method detection limit of mercury in shrimp, crabs and lizards from any site. Mercury levels were also below detection limits in Tilapia, except for specimens collected at Frontera Creek, allegedly contaminated with mercury. However, mercury levels ranged from 92–238 g/kg (wet weight) in Tarpon, a predaceous fish that feeds on smaller fish. Few of the birds had detectable levels of mercury. Our results indicate relatively low concentrations of mercury in biota collected in all of the three estuaries at most trophic levels, although 10 of 12 Tarpon fillet samples from Frontera had detectable mercury compared to 3 of 12 fillet samples for the other two lagoons.This project was partially funded by an EPA consent decree (Dynamac, Inc.) and NIEHS grant (ESO 5022) to EOSHI.     

Establishing a Context for River Rehabilitation, North Fork Gunnison River, Colorado

Initial river rehabilitation efforts along the North Fork Gunnison River in Colorado focused on the use of in-stream structures and channel stabilization to create a single-thread channel with pools along a braided river. These efforts were based on the assumption that the river’s braided planform results primarily from land use during the past century. In order to establish a context for further rehabilitation, we evaluated the possibility that the river might be braided as a result of processes independent of land use. We estimated volume, grain-size distribution, and lithology of sediment sources along the river corridor and evaluated the planform stability of the river during the past century using historical sources, aerial photographs covering 1939–1997, and comparison of bankfull discharge and gradient in the study area to values published for braided and meandering rivers. Our results indicate that the North Fork Gunnison River has been primarily braided in its lower reaches during the past few hundred years, although the channel planform tends toward a single-thread channel during decades of lower precipitation and discharge. Although land use is not the primary cause of braiding along the North Fork Gunnison River, it has decreased channel stability, and rehabilitation efforts should be designed to reduce these effects. Our results illustrate the importance of planning river rehabilitation measures within a historical context that accounts for both catchment-scale and reach-scale controls on channel processes and planform.     

Lethal and sublethal effects of a simulated salt brine effluent on adults and subadults of the shrimps Penaeus setiferus and P. aztecus

Lethal and certain sublethal effects of salt brines on adults and subadults of two species of penaeid shrimps, Penaeus setiferus and P. aztecus, were examined to evaluate the potential impact of ocean disposal of brine from solution mining of salt domes. Brines, prepared from dome salt or synthetic sea salt diluted with Brazos River (Texas, USA) water or deionized water, were mixed with seawater and dalivered from a proportional diluter to shrimp held (usually) at 25°C. For each combination of species, salt, and diluent, 90-individual trials were conducted in the fall and spring. The effects of temperature were evaluated separately. Median lethal time was strongly dose-dependent: Median lethal concentrations at 48 and 96 h were 654±42 (95% confidence interval) and 540±41 mOsm kg^-1 above ambient seawater, respectively, well above the worst-case predictions for the brine-disposal area. Salt type, diluent type, season or species did not significantly affect brine lethality. Mortality was higher for both species at 30°C and lower for P. setiferus and higher for P. aztecus below 25°C. Lethal brine doses produced tachycardia after 6 (P. setiferus) or 12 h (P. aztecus) of brine exposure. Opacity of abdominal muscles increased with brine concentration. Lethal brine concentrations evoked hyperactivity after 0.75–1.5 h of exposure, significant failure to orient after 6 h and a reduction in general activity after 12 h. Behavior and osmoregulation suggest higher sensitivities to brines made with dome salt or river water and in shrimp tested during the cool seasons.     

Fetal liver alanine: Glyoxylate aminotransferase and the prenatal diagnosis of primary hyperoxaluria type 1

Primary hyperoxaluria type 1 (PH1) is caused by a deficiency of the hepatic peroxisomal enzyme alanine: glyoxylate aminotransferase (AGT, EC 2.6.1.44) (Danpure and Jennings, FEBS Lett., 201 , 20–24, 1986). The activity of AGT has been measured in fetal livers of gestational age 14–21 weeks. Activity increases up to 17 weeks and then levels off between 17 and 21 weeks. At this time, the mean AGT activity is about 30 per cent of the mean normal postnatal level. As in adult liver, the AGT enzyme activity and the AGT immunoreactive protein are peroxisomal. Prenatal diagnosis has been performed by measuring AGT enzyme activity and immunoreactive AGT protein on liver biopsies from two fetuses at risk for primary hyperoxaluria type 1. One was unaffected and one was affected.     

First-trimester diagnosis of hunter syndrome: Very low iduronate sulphatase activity in chorionic villi from a heterozygous female fetus

Iduronate sulphatase activity was determined in uncultured chorionic villi from four fetuses at risk for Hunter syndrome. AH fetuses were shown to be female by chromosome analysis. Biopsy material from three fetuses showed iduronate sulphatase activity within normal limits whilst the fourth fetus showed activity reduced to 7 per cent of our control mean. The importance of fetal sexing in prenatal diagnosis of this condition is emphasized as female carrier fetuses may show iduronate sulphatase activity reduced to levels observed in affected males.