Regional evaluation of mass element fluxes: Geomon network of small catchments

Monitoring of element flows and their mass balances in the natural unit of the biosphere represented by a small catchment appears to be the most suitable for the solution of complex problems such as the deterioration of environmental quality. Long-term collection of comparable biogeochemical data, which will enable the identification of environmental trends, is the main aim of the GEOMON network. One of the most important applications of these data will be the calculation of critical loads. The Czech Geological Survey possesses data from 44 catchments in the former Czechoslovakia. Qualitative and quantitative data on inputs in atmospheric deposition and outputs via surface runoff are available. Simple input-output mass balances yield estimates of total accumulation or loss of individual components. Some examples of interpretations of the GEOMON data, especially in connection with different bedrock types, will be given.     

Norovirus Outbreaks on Commercial Cruise Ships: A Systematic Review and New Targets for the Public Health Agenda

Noroviruses are recognized as the leading cause of human acute viral gastroenteritis worldwide. The rate of outbreaks on cruise ships has grown significantly in recent years. Given the potentially harmful consequences of outbreaks for passengers and crewmembers and the subsequently high costs for cruise companies, disease outbreaks on cruise ships represent a serious public health issue. The aim of our study was to systematically review published studies related to Norovirus outbreaks on commercial cruise ships. We searched the PubMed and Scopus scientific databases. We included eligible studies published from January 1990 to July 2013 that were written in English and described infectious episodes involving at least two passengers and/or crewmembers on a commercial cruise ship. As a result, 15 studies and seven reviews met the inclusion criteria, describing a total of 127 outbreaks. The majority of the cases were reported in Europe and the USA, affecting <1 to 74 % of the embarked passengers. In the majority of the studies, stool samples and/or serum specimens from ill passengers were collected and tested for laboratory confirmation. Twelve studies reported that an ad-hoc questionnaire was administered. Fifteen studies investigated the possible source of infection which was contaminated food in the majority of cases. Our findings suggest a strong need for the monitoring and implementation of preventive measures in semi-closed communities, such as cruise ships. It would be advisable to strengthen all relevant initiatives in order to improve the detection of, response to and control of Norovirus outbreaks on cruise ships.     

Assessment of Gastroenteric Viruses from Wastewater Directly Discharged into Uruguay River,Uruguay

The aim of this study was to assess the viral contamination of group A rotavirus (RVA), norovirus (NoV), and human astrovirus (HAstV) in sewage directly discharged into Uruguay River and to characterize RVA genotypes circulating in Uruguay. For this purpose, sewage samples (n = 96) were collected biweekly from March 2011 to February 2012 in four Uruguayan cities: Bella Unión, Salto, Paysandú, and Fray Bentos. Each sample was concentrated by ultracentrifugation method. Qualitative and quantitative RT-PCR for RVA, NoV, and HAstV were performed. A wide dissemination of gastroenteric viruses was observed in the sewage samples analyzed with 80 % of positivity, being NoV (51 %) the most frequently detected followed by RVA with a frequency of 49 % and HAstV with 45 %. Genotypes of RVA were typed using multiplex semi-nested RT-PCR as follows: P[8] (n = 15), P[4] (n = 8), P[10] (n = 1), P[11] (n = 1), G2 (n = 29), and G3 (n = 2). The viral load ranged from 10³ to 10⁷ genomic copies/liter, and they were detected roughly with the same frequency in all participant cities. A peak of RVA and HAstV detection was observed in colder months (June to September), whereas no seasonality was observed for NoV. This study demonstrates for the first time, the high degree of gastroenteric viral contamination in the country; highlighting the importance of developing these analyses as a tool to determine the viral contamination in this hydrographic boundary region used by the local populations for recreation and consumption, establishing an elevated risk of gastroenteric diseases for human health.     

Gene expression changes in blood RNA after swimming in a chlorinated pool

Exposure to disinfection by-products(DBP) such as trihalomethanes(THM) in swimming pools has been linked to adverse health effects in humans, but their biological mechanisms are unclear. We evaluated short-term changes in blood gene expression of adult recreational swimmers after swimming in a chlorinated pool. Volunteers swam 40 min in an indoor chlorinated pool. Blood samples were drawn and four THM(chloroform,bromodichloromethane, dibromochloromethane and bromoform) were measured in exhaled breath before and after swimming. Intensity of physical activity was measured as metabolic equivalents(METs). Gene expression in whole blood m RNA was evaluated using Illumina Human HT-12v3 Expression-Bead Chip. Linear mixed models were used to evaluate the relationship between gene expression changes and THM exposure. Thirty-seven before-after pairs were analyzed. The median increase from baseline to after swimming were: 0.7 to 2.3 for MET, and 1.4 to 7.1 μg/m~3 for exhaled total THM(sum of the four THM).Exhaled THM increased on average 0.94 μg/m~3 per 1 MET. While 1643 probes were differentially expressed post-exposure. Of them, 189 were also associated with exhaled levels of individual/total THM or MET after False Discovery Rate. The observed associations with the exhaled THM were low to moderate(Log-fold change range:-0.17 to 0.15). In conclusion, we identified short-term gene expression changes associated with swimming in a pool that were minor in magnitude and their biological meaning was unspecific. The high collinearity between exhaled THM levels and intensity of physical activity precluded mutually adjusted models with both covariates. These exploratory results should be validated in future studies.     

Proposal for new quantitative eco-design indicators: a first case study

Eco-design is a valuable approach in order to reduce the environmental impact associated with a product system, by introducing environmental considerations early on in its design. Different strategies are possible for the implementation of eco-design, depending on the intended goals as well as the characteristics of the products. The present work proposes a series of eco-design indicators and tests to what extent the application of these simple indicators provides a reliable indication of the reduction of environmental impact, as measured by commonly employed Life Cycle Impact Assessment (LCIA) indicators. The product chosen for the case study was a water source heat pump. Two of the newly-developed indicators were applied and compared to LCIA indicators, focussing on design for disassembly and for recycling. A good and robust correlation was found, providing support to the thesis that these simple eco-design indicators can be used as a proxy to quickly and effectively gauge the environmental improvements introduced in a product system at the design stage.     

The response of Trissolcus basalis to footprint contact kairomones from Nezara viridula females is mediated by leaf epicuticular waxes

Chemical footprints left behind by true bugs are perceived as contact kairomones by scelionid egg parasitoids. Female wasps encountering a contaminated artificial substrate display a characteristic arrestment posture, holding the body motionless and antennating the surface. In the system Nezara viridula (L.) and its egg parasitoid Trissolcus basalis (Wollaston), previous studies have shown that the kairomone mediating such behavior is part of N. viridula’s cuticular hydrocarbons (CHC) and furthermore that the wasp’s ability to discriminate host male and female footprints is mainly based on the presence/absence of nonadecane (nC₁₉). In this study, the effect of epicuticular waxes of leaves of broad bean, Vicia faba, on wasp responses to footprints of N. viridula females were investigated. Approximately 20% of T. basalis females displayed an arrestment posture when released on the adaxial leaf surfaces of broad bean plants with intact wax layer and without host chemical contamination; whereas ∼70% of wasps displayed the arrestment posture when intact leaves were contaminated by host female footprints. Adaxial leaf surfaces of broad bean plants dewaxed with an aqueous solution of gum arabic and afterwards contaminated by N. viridula females induced arrestment responses in about 10% of female wasps; the same percentage of arrestment (10%) was observed when the wasps were released on leaves contaminated by host females and subsequently dewaxed. The side of the polymer film that was appressed to the leaf surface, peeled from the contaminated leaves, induced an arrestment posture in about 95% of observed wasps. Scanning electron microscopy (SEM) revealed that the epicuticular waxes occurred as a film densely crystallized as irregularly shaped platelets with spherical granules randomly distributed. These findings demonstrated that epicuticular waxes of broad bean leaves can mediate the foraging behavior of T. basalis females by absorbing contact kairomones of the host.     

Cancer and occupational exposure to pesticides: a bibliometric study of the past 10 years

Environmental Science and Pollution Research - Occupational exposure to pesticides has been identified as a major trigger of the development of cancer. Pesticides can cause intoxication in the...     

Reuse of wood ash from biomass combustion in non-structural concrete: mechanical properties,durability, and eco-efficiency

Journal of Material Cycles and Waste Management - Wood biomass fly ash can contribute to a more sustainable urban infrastructure, but requires efficient recycling strategies. This paper...     

Chemical,Morphological, Thermal and Technological Properties of Acetylated White Inhambu Starch

Journal of Polymers and the Environment - Starch is the main predominant food reserve in plants. Its characteristics stand out from other carbohydrates providing it with several industrial...     

Fast Preparation of Flexible Wet-Resistant and Biodegradable Films From a Stable Suspension of Xylan/Chitosan Polyelectrolyte Complexes

Journal of Polymers and the Environment - Novel hemicellulose-based films from polyelectrolyte complexes (PECs) of chitosan and xylan from sugar cane bagasse were prepared and characterized. PEC...