The occurrence and determination of PCH3-containing compounds in surface water

A procedure has been developed in which the presence of PCH₃-containing compounds in surface waters can be detected in a sensitive and specific way. The compounds of interest are hydrolysed, yielding methylphosphonic acid as a common breakdown product. This acid is concentrated using an anion-exchange column and subsequently transformed into its dimethyl ester by diazomethane. After a clean-up on a microsilica gel column the ester is analysed by means of gas chromatography using a thermionic detector as a specific phosphorus detector. The lowest detectable concentration is approximately 1 nmol of a PCH₃-containing compound per litre of water.Samples from the rivers Rhine and Meuse and from two lakes in the Netherlands were analysed. Concentration levels of 0.1 – 1 μg/l of PCH₃-containing compounds expressed as methylphosphonic acid were found. The origin of the compounds and their possible accumulation in the environment are discussed.     

Ontogenic changes of amino acid composition in planktonic crustacean species

Changes in amino acid composition (AAC) during ontogeny of some planktonic crustacean species commonly found in fresh and brackish coastal waters were compared. For these comparisons two calanoid copepods (Eurytemora velox and Calanipeda aquae-dulcis), two cyclopoid copepods (Diacyclops bicuspidatus odessanus and Acanthocyclops robustus) and two Daphnia (Daphnia pulicaria and Daphnia magna) species were selected. A discriminant analysis was performed to determine whether there were significant differences between the AAC of the different stages of each species. Results show gradual changes in AAC during ontogeny of the copepod species. Calanoids showed the greatest differences in AAC between stages, followed by cyclopoids. Gradual changes in AAC were due to the increase in some amino acids such as alanine, valine, glutamic acid, glycine, arginine, proline and tyrosine from nauplii to adults. The latter showed a remarkable increase in all copepod species. In contrast, Daphnia species showed a relatively constant AAC during development, with only minor changes being detected, and not related with ontogeny. Differences in the physico-chemical variables of the lagoons do not seem to be the cause of copepod ontogenic changes in AAC. Data suggest that AAC differences found between stages of copepod species could indicate a gradual change in diet during the life cycle of these copepods.     

Induction of a reproductive-specific cuticular hydrocarbon profile by a juvenile hormone analog in the termite <Emphasis Type="Italic">Zootermopsis nevadensis</Emphasis>

Establishment and maintenance of the reproductive division of labor within social insect colonies relies on clear communication between nestmates. Fertile members convey their status to prevent others from becoming reproductively active. Recent findings in some basal termites indicate that cuticular hydrocarbon profiles may indicate reproductive state, but there is little evidence to show a direct link between reproductive status and hydrocarbon production—a prerequisite for an “honest” fertility signal. Here, we report that the putative signaling mechanism is influenced by juvenile hormone (JH), a primary regulator of gonadal development and activity in insects. Topical application of a JH-analog (pyriproxyfen) to reproductively inactive alates of the basal dampwood termite Zootermopsis nevadensis induced both females and males to express significantly more of a reproductive-specific hydrocarbon (6,9,17-tritriacontatriene). However, the JH-analog did not significantly enhance gonadal development or activity in treated termites beyond what is usually observed in maturing alates released from the inhibitory stimuli of their natal nest. These results suggest that a rise in JH following disinhibition drives the expression of reproductive-specific hydrocarbons, but that an individual’s hydrocarbon profile is not directly linked to its gonadal state. Rather than directly driving the expression of reproductive-specific hydrocarbons, the gonads may act indirectly through their influence on circulating JH.     

OPERA: a New High-Resolution Energy System Model for Sector Integration Research

Environmental Modeling & Assessment - This article introduces and describes OPERA, a new technology-rich bottom-up energy system optimization model for the Netherlands. We give a detailed...     

Why are larvae of the social parasite wasp <Emphasis Type="Italic">Polistes sulcifer</Emphasis> not removed from the host nest?

A challenge for parasites is how to evade the sophisticated detection and rejection abilities of potential hosts. Many studies have shown how insect social parasites overcome host recognition systems and successfully enter host colonies. However, once a social parasite has successfully usurped an alien nest, its brood still face the challenge of avoiding host recognition. How immature stages of parasites fool the hosts has been little studied in social insects, though this has been deeply investigated in birds. We look at how larvae of the paper wasp obligate social parasite Polistes sulcifer fool their hosts. We focus on cuticular hydrocarbons (CHCs), which are keys for adult recognition, and use behavioral recognition assays. Parasite larvae might camouflage themselves either by underproducing CHCs (odorless hypothesis) or by acquiring a chemical profile that matches that of their hosts. GC/MS analyses show that parasite larvae do not have lower levels of CHCs and that their CHCs profile is similar to the host larval profile but shows a reduced colony specificity. Behavioral tests show that the hosts discriminate against alien conspecific larvae from different colonies but are more tolerant towards parasite larvae. Our results demonstrate that parasite larvae have evolved a host larval profile, which overcomes the host colony recognition system probably because of the lower proportion of branched compounds compared to host larvae. In some ways, this is a similar hypothesis to the odorless hypothesis, but it assumes that the parasite larvae are covered by a chemical blend that is not meaningful to the host.     

In Flanders Fields: De/politicization and Democratic Debate on a GM Potato Field Trial Controversy in News Media

In May 2011, a highly mediatized direct action in the form of a “field liberation” took place in Flanders, Belgium, against a field trial of genetically modified potatoes. This direct action and its aftermath revealed the conflictual and antagonistic nature of the genetically modified organisms debate in Flanders. Consequently, it serves as a particularly suitable case to investigate the post-political thesis with regard to environmental discourse and politics. The aim of this paper is to investigate in what ways news media in their reporting contribute to processes of depoliticization and, resultantly, impede a democratic debate on the issues at stake. This paper attempts to provide an answer to this question based on a critical discourse analysis of the reporting by Flanders’ two generalist, elite newspapers, De Standaard and De Morgen and the alternative online news site DeWereldMorgen. The findings reveal the existence of three ideological cultures, which are characterized by different degrees of de/politicization. We conclude by discussing how this study adds to our understanding of the relation between the depoliticization of environmental discourse, democratic debate and twenty-first-century news media.     

Dissipation of glyphosate and aminomethylphosphonic acid in water and sediment of two Canadian prairie wetlands

Glyphosate [N-(phosphonomethyl)glycine] is the active ingredient of several herbicide products first registered for use in 1974 under the tradename Roundup. The use of glyphosate-based herbicides has increased dramatically over the last two decades particularly in association with the adoption of glyphosate-tolerant crops. Glyphosate has been detected in a range of surface waters but this is the first study to monitor its fate in prairie wetlands situated in agricultural fields. An ephemeral wetland (E) and a semi-permanent wetland (SP) were each divided into halves using a polyvinyl curtain. One half of each wetland was fortified with glyphosate with the added mass simulating an accidental direct overspray. Glyphosate dissipated rapidly in the water column of the two prairie wetlands studied (DT(50) values of 1.3 and 4.8 d) which may effectively reduce the impact of exposure of aquatic biota to the herbicide. Degradation of glyphosate to its major metabolite aminomethylphosphonic acid (AMPA) and sorption of the herbicide to bottom sediment were more important pathways for the dissipation of glyphosate from the water column than movement of the herbicide with infiltrating water. Presently, we are not aware of any Canadian guidelines for glyphosate residues in sediment of aquatic ecosystems. Since a substantial portion of glyphosate entering prairie wetlands will become associated with bottom sediments, particularly in ephemeral wetlands, guidelines would need to be developed to assess the protection of organisms that spend all or part of their lifecycle in sediment.     

Modeling of methane oxidation in landfill cover soil using an artificial neural network

Knowing the fraction of methane (CH₄) oxidized in landfill cover soils is an important step in estimating the total CH₄ emissions from any landfill. Predicting CH₄ oxidation in landfill cover soils is a difficult task because it is controlled by a number of biological and environmental factors. This study proposes an artificial neural network (ANN) approach using feedforward backpropagation to predict CH₄ oxidation in landfill cover soil in relation to air temperature, soil moisture content, oxygen (O₂) concentration at a depth of 10 cm in cover soil, and CH₄ concentration at the bottom of cover soil. The optimum ANN model giving the lowest mean square error (MSE) was configured from three layers, with 12 and 9 neurons at the first and the second hidden layers, respectively, log-sigmoid (logsig) transfer function at the hidden and output layers, and the Levenberg-Marquardt training algorithm. This study revealed that the ANN oxidation model can predict CH₄ oxidation with a MSE of 0.0082, a coefficient of determination (R ²) between the measured and predicted outputs of up to 0.937, and a model efficiency (E) of 0.8978. To conclude, further developments of the proposed ANN model are required to generalize and apply the model to other landfills with different cover soil properties.

Implications:

To date, no attempts have been made to predict the percent of CH₄ oxidation within landfill cover soils using an ANN. This paper presents modeling of CH₄ oxidation in landfill cover soil using ANN based on field measurements data under tropical climate conditions in Malaysia. The proposed ANN oxidation model can be used to predict the percentage of CH₄ oxidation from other landfills with similar climate conditions, cover soil texture, and other properties. The predicted value of CH₄ oxidation can be used in conjunction with the Intergovernmental Panel on Climate Change (IPCC) First Order Decay (FOD) model by landfill operators to accurately estimate total CH₄ emission and how much it contributes to global warming.     

Preparation and Properties of Propylene Glycol Plasticized Wheat Protein Isolate Novel Green Films

Novel bio-based green films were prepared using wheat protein isolate (WPI) by solution casting method using Propylene Glycol as a plasticizer for packaging applications. The effect of the plasticizer content (10, 15, 20 and 25 wt%) on mechanical properties (tensile strength, young’s modulus and  % of elongation) was investigated. A thermal degradation and phase transition of the prepared WPI was assessed by means of TGA and DSC analysis. The results showed that the tensile strength and young’s modulus decreased and  % of elongation increased with increasing PG content. The ATR-FTIR and SEM were used for structural characterization and morphology of the films, respectively. FTIR studies reveals that the intensity of the bands corresponding to the amide groups increases with increasing PG content tending to increase protein–PG interactions. Further, the glass transition temperature was decreased and the thermal stability of the WPI was found to be increased by plasticization. The overall thermal stability of the films was improved and is attributed to the increase in mobility of the polymer chains.