Microbial characterization of nitrification in a shallow, nitrogen-contaminated aquifer, Cape Cod, Massachusetts and detection of a novel cluster associated with nitrifying Betaproteobacteria

Groundwater nitrification is a poorly characterized process affecting the speciation and transport of nitrogen. Cores from two sites in a plume of contamination were examined using culture-based and molecular techniques targeting nitrification processes. The first site, located beneath a sewage effluent infiltration bed, received treated effluent containing O₂ (> 300 µM) and NH₄⁺ (51–800 µM). The second site was 2.5 km down-gradient near the leading edge of the ammonium zone within the contaminant plume and featured vertical gradients of O₂, NH₄⁺, and NO₃⁻ (0–300, 0–500, and 100–200 µM with depth, respectively). Ammonia- and nitrite-oxidizers enumerated by the culture-based MPN method were low in abundance at both sites (1.8 to 350 g^− 1 and 33 to 35,000 g^− 1, respectively). Potential nitrifying activity measured in core material in the laboratory was also very low, requiring several weeks for products to accumulate. Molecular analysis of aquifer DNA (nested PCR followed by cloning and 16S rDNA sequencing) detected primarily sequences associated with the Nitrosospira genus throughout the cores at the down-gradient site and a smaller proportion from the Nitrosomonas genus in the deeper anoxic, NH₄⁺ zone at the down-gradient site. Only a single Nitrosospira sequence was detected beneath the infiltration bed. Furthermore, the majority of Nitrosospira-associated sequences represent an unrecognized cluster. We conclude that an uncharacterized group associated with Nitrosospira dominate at the geochemically stable, down-gradient site, but found little evidence for Betaproteobacteria nitrifiers beneath the infiltration beds where geochemical conditions were more variable.     

Non-market valuation of off-highway vehicle recreation in Larimer County, Colorado: Implications of trail closures

Few economic studies are available to measure off-highway vehicle recreation benefits foregone when trails must be closed to protect the environment. This paper estimates the non-market benefits associated with off-highway vehicle (OHV) recreation on National Forest lands in Larimer County, Colorado. We use a contingent valuation model (CVM) to estimate benefits to OHV users, which includes dirt bike riders, all terrain vehicle (ATV) riders, and 4-wheel drive (4 × 4) users. Using CVM we find the mean consumer surplus estimates to be $78 per person per day. These results are consistent with the few previous estimates of OHV recreation benefits. This equates to a per trail per summer consumer surplus of at least between $219,467 and $296,876, and a county level surplus per summer to be at least between $796,447 and $1,077,367. These benefits can be compared to environmental costs to obtain a more complete picture of the effects of trail closure, as well as the negative spillovers to non-motorized users.     

Modeling toxic compounds from nitric oxide emission measurements

Determining the amount and rate of degradation of toxic pollutants in soil and groundwater is difficult and often requires invasive techniques, such as deploying extensive monitoring well networks. Even with these networks, degradation rates across entire systems cannot readily be extrapolated from the samples. When organic compounds are degraded by microbes, especially nitrifying bacteria, oxides or nitrogen (NO_x) are released to the atmosphere. Thus, the flux of nitric oxide (NO) from the soil to the lower troposphere can be used to predict the rate at which organic compounds are degraded. By characterizing and applying biogenic and anthropogenic processes in soils the rates of degradation of organic compounds. Toluene was selected as a representative of toxic aromatic compounds, since it is inherently toxic, it is a substituted benzene compound and is listed as a hazardous air pollutant under Section 12 of the Clean Air Act Amendments of 1990. Measured toluene concentrations in soil, microbial population growth and NO fluxes in chamber studies were used to develop and parameterize a numerical model based on carbon and nitrogen cycling. These measurements, in turn, were used as indicators of bioremediation of air toxic (i.e. toluene) concentrations. The model found that chemical concentration, soil microbial abundance, and NO production can be directly related to the experimental results (significant at P < 0.01) for all toluene concentrations tested. This indicates that the model may prove useful in monitoring and predicting the fate of toxic aromatic contaminants in a complex soil system. It may also be useful in predicting the release of ozone precursors, such as changes in reservoirs of hydrocarbons and oxides of nitrogen. As such, the model may be a tool for decision makers in ozone non-attainment areas.     

What catch data can tell us about the status of global fisheries

The only available data set on the catches of global fisheries are the official landings reported annually by the Food and Agriculture Organization of the United Nations (FAO). Attempts to detect and interpret trends in these data have been criticized as being both technically and conceptually flawed. Here, we explore and refute these claims. We show explicitly that trends in catch data are not an artifact of the applied method and are consistent with trends in biomass data of fully assessed stocks. We also show that, while comprehensive stock assessments are the preferred method for evaluating single stocks, they are a biased subsample of the stocks in a given area, strongly underestimating the percentage of collapsed stocks. We concur with a recent assessment-based analysis by FAO that the increasing trends in the percentage of overexploited, depleted, and recovering stocks and the decreasing trends in underexploited and moderately exploited stocks give cause for concern. We show that these trends are much more pronounced if all available data are considered.     

中国长江三角洲地区电力行业SO2排放控制的经济分析

总结了中国SO2排放总量控制与排放权交易政策研究与实施3个阶段的主要内容与成果。使用YRDEGS线性规划优化模型对在长江三角洲地区电力行业实施SO2排放权交易所能产生的SO2控制费用的节省、安装FGD装机容量的变化、可引起的脱硫变动成本的节约、SO2排放许可的市场需求、不同交易方式所引起的脱硫费用变化进行分析,得到以下主要结论：若发电量保持在2000年的水平,实现SO2减排20％的目标,采用排污交易脱硫费用将会节省38％,可以少安装FGD的机组装机容量达6．3GW;随着发电量的增加,对SO2排放许可指标的市场需求呈增长趋势,发电量的改变比逐步严格的SO2减排要求更会增加区域SO2减排的负担;当SO2排放削减率为20％时,在不实施排污交易时脱硫费用约为30亿～45亿元;实施跨省排污交易以电厂为交易单位时费用最低,约为6亿～28．5亿元,脱硫费用可以节省16．5亿～24亿元。     

INPUTS OF COPPER‐BASED CROP PROTECTANTS TO COASTAL CREEKS FROM PASTICULTURE RUNOFF1

ABSTRACT: Inputs of copper‐based crop protectants from tomato fields grown under plastic mulch agriculture (plasticulture) to an estuarine creek were investigated. Copper was measured in runoff from diverse land‐uses including conventional agriculture, plasticulture, residences, and natural areas. Water column and sediment copper concentrations were measured in plasticulture and control (nonagriculture) watersheds. Copper concentrations in plasticulture‐impacted creeks exceeded background levels episodically. High concentrations occurred during or immediately after runoff‐producing rains. Concentrations of 263 μg/L total copper and 126 μg/L dissolved copper were measured in a tidal creek affected by plasticulture; concentrations exceeded the shellfish LC₅₀ values and the water quality criteria of 2.9 μg/L dissolved copper. Control watersheds indicated background water column levels of ≤ 4 μg/L dissolved copper with similar copper levels during periods with and without rain. The copper concentrations in tomato plasticulture field runoff itself contained up to 238 μg/L dissolved copper. Copper concentrations in runoff from other land‐uses were less than 5 μg/L dissolved copper. Creek sediment samples adjacent to a plasticulture field contained significantly higher copper concentrations than sediments taken from nonplasticulture watersheds.     

Performance Track Places Trust in the Carrot over the Stick

A new voluntary program from the Environmental Protection Agency (EPA) rewards companies that go beyond compliance, thereby helping businesses that are top environmental performers. © 2001 John Wiley & Sons, Inc.