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81.
Ana Teresa Luís Nuno Dures Salomé Fernandes Pinheiro de Almeida Eduardo Ferreira da Silva 《环境科学学报(英文版)》2016,28(4):215-226
Aljustrel mines were classified as having high environmental hazard due to their large tailings volume and high metal concentrations in waters and sediments.To assess acid mine drainage impacted systems whose environmental conditions change quickly,the use of biological indicators with short generation time such as diatoms is advantageous.This study combined geochemical and diatom data,whose results were highlighted in 3 groups:Group 1,with low p H(1.9–5.1)and high metal/metalloid(Al,As,Cd,Co,Cu,Fe,Mn,Ni,Pb,Zn;0.65–1032 mg/L)and SO_4(405–39124 mg/L)concentrations.An acidophilic species,Pinnularia aljustrelica,was perfectly adapted to the adverse conditions;in contrast,teratological forms of Eunotia exigua were found,showing that metal toxicity affected this species.The low availability of metals/metalloids in sediments of this group indicates that metals/metalloids of the exchangeable fractions had been solubilized,which in fact enables metal/metalloid diatom uptake and consequently the occurrence of teratologies;Group 2,with sites of near neutral p H(5.0–6.8)and intermediate metal/metalloid(0.002–6 mg/L)and SO_4(302–2179 mg/L)concentrations;this enabled the existence of typical species of uncontaminated streams(Brachysira neglectissima,Achnanthidium minutissimum);Group 3,with samples from unimpacted sites,showing low metal/metalloid(0–0.8 mg/L)and SO_4(10–315 mg/L)concentrations,high pH(7.0–8.4)and Cl contents(10–2119 mg/L)and the presence of brackish to marine species(Entomoneis paludosa).For similar conditions of acidity,differences in diversity,abundance and teratologies of diatoms can be explained by the levels of metals/metalloids. 相似文献
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Renata de Lima Anderson do Espirito Santo Pereira Raquel Martins Porto Leonardo Fernandes Fraceto 《Journal of Polymers and the Environment》2011,19(1):196-202
This work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an
attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions
containing three different concentrations of nanoparticles (5.4, 54 and 540 μg/mL, polymer mass/volume of solution). The nanoparticles
were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of
the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95 nm, a zeta potential
of −20 mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared
with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index
in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models
did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible
PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations
employed. The results provide new information concerning the toxic effects of particles produced using PLGA. 相似文献
86.
Navarro Betina Ludwig Challiol Adriana Zemiani Bortoloti Mauricio Aparecido Kuroda Emília Kiyomi Fernandes Fernando 《Journal of Material Cycles and Waste Management》2022,24(6):2470-2482
Journal of Material Cycles and Waste Management - This research aimed to evaluate the production of biogas from anaerobic digestion (AD) using swine manure inoculum associated with different... 相似文献
87.
Dias Marcela França Leroy-Freitas Deborah Machado Elayne Cristina da Silva Santos Leticia Leal Cintia Dutra da Rocha Fernandes Gabriel de Araújo Juliana Calábria 《Environmental science and pollution research international》2022,29(24):36088-36099
Environmental Science and Pollution Research - Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and... 相似文献
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The relationship among sugar concentrations, microbial community and physical variables (precipitation and soil temperature) was investigated in a ryegrass soil from January 2004 to January 2005. Mono- and disaccharide sugars were extracted using a mixture of dichloromethane and methanol and analyzed as their TMS derivatives by GC-MS. Changes in microbial community were assessed using phospholipid and neutral lipid fatty acids (PLFA and NLFA, respectively) analysis. The results of a one-year study showed that the seasonal variability of sugar contents found in the soil samples is mainly related to biomass or nutritional status of the fungal community. The increase in sucrose and fructose exudation by plant roots in the beginning of the growing season (early spring) may be responsible for the highest fungal biomass amount (PLFAs) observed in this study. Fungal storage lipid abundances (NLFAs) peaked in summer, during the same period that the highest concentrations of mannitol and trehalose were detected. This is consistent with these two sugars being stress-induced fungal metabolites, produced due to the low soil moisture observed during this season. In contrast, bacterial community growth seems to be more dependent on plant substrate than on physical variables, since the strongest decrease in bacterial biomass amounts (PLFAs) was found after cutting of the ryegrass field in early July. 相似文献
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Ana Vicente Ana Calvo Ana P. Fernandes Teresa Nunes Cristina Monteiro Casimiro Pio Célia Alves 《环境科学学报(英文版)》2017,29(3):122-131
In summer 2010, twenty eight(14 PM_(2.5)samples plus 14 samples PM_(2.5–10)) smoke samples were collected during wildfires that occurred in central Portugal. A portable high-volume sampler was used to perform the sampling, on quartz fibre filters of coarse(PM_(2.5–10)) and fine(PM_(2.5)) smoke samples. The carbonaceous content(elemental and organic carbon) of particulate matter was analysed by a thermal–optical technique. Subsequently, the particulate samples were solvent extracted and fractionated by vacuum flash chromatography into three different classes of organic compounds(aliphatics, polycyclic aromatic hydrocarbons(PAHs) and carbonyl compounds). The organic speciation was performed by gas chromatography–mass spectrometry(GC–MS). Emissions were dominated by the fine particles, which represented around 92% of the PM_(10). A clear predominance of carbonaceous constituents was observed, with organic to elemental carbon(OC/EC)ratios ranging between 1.69 and 245 in both size fractions. The isoprenoid ketone6,10,14-trimethyl-2-pentadecanone, a tracer for secondary organic aerosol formation, was one of the dominant constituents in both fine and coarse particles. Retene was the most abundant compound in all samples. Good correlations were obtained between OC and both aliphatic and PAH compounds. Pyrogenic processes, thermal release of biogenic compounds and secondary processing accounted for 97% of the apportioned PM_(2.5)levels. 相似文献