Detection and isolation of fetal cells from maternal blood using the flourescence-activated cell sorter (FACS)

The presence of fetal cells in the maternal circulation during pregnancy has been suggested by repeated observations of small numbers of cells containing Y chromatin or a Y chromosome in the blood of pregnant women. With the fluorescence-activitated cell sorter (FACS), we have used antibodies to a paternal cell surface (HLA) antigen, not present in the mother, to select fetal cells from the lymphocyte fractions of a series of maternal blood samples, collected as early as 15 weeks of gestation. These sorted cells have been examined for a second paternal genetic marker, Y chromatin. Y chromatin-containing cells were found among the sorted cells from prenatal maternal blood specimens in 8 pregnancies subsequently producing male infants whose lymphocytes reacted with the same antibodies to paternal antigen used for sorting with the FACS. In each of 17 pregnancies resulting in male infants who failed to inherit the antigen detected by the antibodies used for cell sorting, Y chromatin-containing cells were not found prenatally. The use of two paternal genetic markers, a cell surface antigen and nuclear Y chromatin, to identify fetal cells in maternal blood permits us to conclude that these cells are present in the mother's circulation, as early as 15 weeks gestation. Further development of the techniques reported here could lead to widespread screening of maternal blood samples during pregnancy for detection of fetal genetic abnormalities.     

Climate change,phenology, and butterfly host plant utilization

Knowledge of how species interactions are influenced by climate warming is paramount to understand current biodiversity changes. We review phenological changes of Swedish butterflies during the latest decades and explore potential climate effects on butterfly–host plant interactions using the Orange tip butterfly Anthocharis cardamines and its host plants as a model system. This butterfly has advanced its appearance dates substantially, and its mean flight date shows a positive correlation with latitude. We show that there is a large latitudinal variation in host use and that butterfly populations select plant individuals based on their flowering phenology. We conclude that A. cardamines is a phenological specialist but a host species generalist. This implies that thermal plasticity for spring development influences host utilization of the butterfly through effects on the phenological matching with its host plants. However, the host utilization strategy of A. cardamines appears to render it resilient to relatively large variation in climate.     

Development of nanoemulsion from Vitex negundo L. essential oil and their efficacy of antioxidant,antimicrobial and larvicidal activities (Aedes aegypti L.)

Environmental Science and Pollution Research - It is believed that nanoemulsions were emerged as a promising candidate to improve the qualities of natural essential oil towards antimicrobial and...     

Global sustainability: Toward measurement

The widespread interest in the concept of sustainable environment and development has been accompanied by the need to develop useful systems of measurement. We discuss the use of indicators which might be used to assess such conditions. Our characteristics, or criteria, for desirable global sustainability indicators are:

sensitivity to change in time

sensitivity to change across space or within groups

predictive ability

availability of reference or threshold values

ability to measure reversibility or controllability

appropriate data transformation

integrative ability

relative ease of collection and use

We discuss the basis of these characteristics, and examine two categories of indicators (soil erosion and population) and two specific indicators (physical quality of life index and energy imports as a percentage of consumption) for their value as sustainability measures.     

A GIS-based technique for linking landscape characteristics to non-point source nitrogen export potential: implications for contributing areas management

A Nitrate-N Leaching Index (NLI) is calculated and the results indicate that nitrogen loss in the study area occurs through both leaching and surface runoff. A non-linear regression model of trapping efficiency was combined with a first order decay model to examine the impact of soil characteristics, slope, vegetative cover, land use and distance to streams on the spatial pattern of non-point source nitrogen inputs to streams. The model evaluates the statistical significance of each landscape factor and provides an easy interpretation of the landscape delivery ratio of nitrogen based on a pixel-based characterisation of the watershed. The model was validated by comparing the distributions of the observed and estimated monthly nitrogen concentrations. The exploratory GIS-based method presented here can improve the understanding of the impact of landscape characteristics on nitrate-nitrogen contributing areas and therefore assist watershed management efforts.     

Contesting neoliberal knowledge politics in restoration governance: the restorationist’s dilemma

ABSTRACT

The success of ecological restoration efforts is tightly coupled with the effectiveness of many U.S. environmental policies. Yet scholars have raised questions about the ability of restoration to produce intended results. We use a case study of tidal wetland restoration planning in Oregon to examine how neoliberal environmental governance exercises influence through a set of knowledge politics that produces subpar outcomes. We present three main findings: (1) restoration policies produce a restoration economy based on a conception of wetland as commodity (2) practitioners in this restoration economy exhibit competitive behavior resulting in a piecemeal rather than a landscape approach to restoration; and (3) limited monitoring prevents changes to existing policies. Practitioners offer insight into the challenge of treating wetlands as a commodity and call for more monitoring to challenge the assumptions of hegemonic knowledge practices that reinforce a neoliberal environmental governance regime. The divergent ideas of reflexive practitioners, though not yet manifest as action, show where changes to restoration governance might be possible.     

Data integration across borders: a case study of the Abbotsford‐Sumas aquifer (British Columbia/Washington State)1

Abstract: Integrating spatial datasets from diverse sources is essential for cross‐border environmental investigations and decision‐making. This is a little investigated topic that has profound implications for the availability and reliability of spatial data. At present, ground‐water hydrostratigraphic models exist for both the Canadian or for the United States (U.S.) portion of the aquifer but few are integrated across the border. In this paper, we describe the challenges of integrating multiple source, large datasets for development of a ground‐water hydrostratigraphic model for the Abbotsford‐Sumas Aquifer. Growing concerns in Canada regarding excessive withdrawal south of the border and in the U.S. regarding nitrate contamination originating north of the border make this particular aquifer one of international interest. While much emphasis in GIScience is on theoretical solutions to data integration, such as current ontology research, this study addresses pragmatic ways of integrating data across borders. Numerous interoperability challenges including the availability of data, metadata, data formats and quality, database structure, semantics, policies, and cooperation are identified as inhibitors of data integration for cross‐border studies. The final section of the paper outlines two possible solutions for standardizing classification schemes for ground‐water models – once data heterogeneity has been addressed.