Low frequency electromagnetic waves as a supplemental energy source to sustain microbial growth?

Microbial populations in tetra-distilled water collapsed when cultured in a permalloy chamber shielding the populations from the suns and earths electromagnetic field, but thrived when cultured in an ordinary thermostat open to the electromagnetic field. Theoretically, protons in liquid water can be excited at their natural resonance frequencies through Langmuir oscillations and obtain enough kinetic energy to charge the transmembrane potential of a cell. Microbes may be capable of converting this energy into chemical energy to supplement their energy needs.     

Origin of middle rare earth element enrichment in acid mine drainage-impacted areas

The commonly observed enrichment of middle rare earth elements (MREE) in water sampled in acid mine drainage (AMD)-impacted areas was found to be the result of preferential release from the widespread mineral pyrite (FeS₂). Three different mining-impacted sites in Europe were sampled for water, and various pyrite samples were used in batch experiments with diluted sulphuric acid simulating AMD-impacted water with high sulphate concentration and high acidity. All water samples independent on their origin from groundwater, creek water or lake water as well as on the surrounding rock types showed MREE enrichment. Also the pyrite samples showed MREE enrichment in the respective acidic leachate but not always in their total contents indicating a process-controlled release. It is discussed that most probably complexation to sulphite (SO₃ ^2?) or another intermediate S-species during pyrite oxidation is the reason for the MREE enrichment in the normalized REE patterns.     

Measurement of tool internal temperatures in the tool–chip contact region by embedded micro thin film thermocouples

Sensors capable of providing fast and reliable feedback signals for monitoring and control of existing and emerging machining processes are an important research topic, that has quickly gained academic and industrial interest in recent years. Generally, high-precision machining processes are very sensitive to variation in local machining conditions at the tool–workpiece interface and lack a thorough understanding of fundamental thermomechanical phenomena. Existing sensors to monitor the machining conditions are not suitable for robust in-process control as they are either destructively embedded and/or do not possess the necessary spatial and temporal resolution to monitor local tool internal temperatures during machining at the cutting tip/edge effectively. This paper presents a novel approach for assessing transient tool internal temperature fields in the close vicinity of less than 300 μm of the tool cutting edge. A revised array layout of 10 micro thin film micro thermocouples, fabricated using adapted semiconductor microfabrication methods, has been embedded into polycrystalline cubic boron nitride (PCBN) cutting inserts by means of a modified diffusion bonding technique. Scanning electron microscopy was used to examine material interactions at the bonding interface and to determine optimal bonding parameters. Sensor performance was statically and dynamically characterized. They show good linearity, sensitivity and very fast response time. Initial machining tests on aluminum alloys are described herein. The tests have been performed to demonstrate the functionality and reliability of tool embedded thin film sensors, and are part of a feasibility study with the ultimate goal of applying the instrumented insert in hard machining operations. The microsensor array was used for the acquisition of tool internal temperature profiles very close to the cutting tip. The influence of varying cutting parameters on transient tool internal temperature profiles was measured and discussed. With further study, the described instrumented cutting inserts could provide more valuable insight into the process physics and could improve various aspects of machining processes, e.g. reliability, tool life, and workpiece quality.     

Application of next-generation sequencing technologies to conservation of wood-inhabiting fungi

Next-generation sequencing (NGS) has significantly increased knowledge of microbial communities and their distribution. However, it is still not common to apply NGS technology to microbial conservation. We sought to use NGS technologies to evaluate conservation strategies for wood-inhabiting fungi. Evaluating a deadwood experiment 3 years after it was established, we specifically examined which tree species combinations promoted the highest richness of wood-inhabiting fungi. Deadwood enrichment was an effective strategy and logs of 6 tree species, either those with the highest wood-inhabiting fungal α and γ diversity or those with the highest β diversity, maintained >1,000 operational taxonomic units (OTUs) spread over a wide range of taxonomic groups. In comparison, a conservation strategy based only on the results of sporocarp surveys yielded 591 OTUs. This result highlights the need to use NGS approaches to inform microbial conservation strategies. We also determined that 5 tree species with the highest saproxylic beetle γ diversity simultaneously conserved wood-inhabiting fungi. Apart from deadwood volume, we suggest data on deadwood quality and species also be included as indicators, especially for wood-inhabiting fungal diversity, and incorporated quickly in forest assessment and monitoring systems in Central Europe.     

Dose-response relationships in mutagenicity assays including an appropriate positive control group: a multiple testing approach

The objective of mutagenicity assays in regulatory toxicology is the decision on non-mutagenicity or mutagenicity. An inherent problem of statistical tests is the possibility of false decisions, i.e., a mutagenic substance will be falsely labeled as non-mutagenic or a non-mutagenic substance will be falsely labeled as mutagenic. These probabilities of false negative (consumer's risk=type II error) and/or false positive decision (producer's risk=type I error) can be limited by using suitable testing procedures as well as a design including an appropriate positive control. Using the proof of hazard concept the well-known many-to-one procedures with total order restriction for increasing effect differences are used, while using the proof of safety concept procedures on equivalence with total order restriction are discussed. Both approaches are demonstrated on a real data example.     

Differential responses of eubacterial, Mycobacterium, and Sphingomonas communities in polycyclic aromatic hydrocarbon (PAH)-contaminated soil to artificially induced changes in PAH profile

Recent reports suggest that Mycobacterium is better adapted to soils containing poorly bioavailable polycyclic aromatic hydrocarbons (PAHs) compared to Sphingomonas. To study this hypothesis, artificial conditions regarding PAH profile and PAH bioavailability were induced in two PAH-contaminated soils and the response of the eubacterial, Mycobacterium, and Sphingomonas communities to these changed conditions was monitored during laboratory incubation. Soil K3663 with a relatively high proportion of high molecular weight PAHs was amended with phenanthrene or pyrene to artificially change the soil into a soil with a relatively increased bioavailable PAH contamination. Soil AndE with a relatively high proportion of bioavailable low molecular weight PAHs was treated by a single-step Tenax extraction to remove the largest part of the easily bioavailable PAH contamination. In soil K3663, the added phenanthrene or pyrene compounds were rapidly degraded, concomitant with a significant increase in the number of phenanthrene and pyrene degraders, and minor and no changes in the Mycobacterium community and Sphingomonas community, respectively. However, a transient change in the eubacterial community related to the proliferation of several gamma-proteobacteria was noted in the phenanthrene-amended soil. In the extracted AndE soil, the Sphingomonas community initially developed into a more diverse community but finally decreased in size below the detection limit. Mycobacterium in that soil never increased to a detectable size, while the eubacterial community became dominated by a gamma-proteobacterial population. The results suggest that the relative bioavailability of PAH contamination in soil affects bacterial community structure but that the behavior of Mycobacterium and Sphingomonas in soil is more complex than prospected from studies on their ecology and physiology.     

The construct validity of two burnout measures

The present study was carried out among a sample of 667 Dutch nurses and assesses three aspects of the construct validity of the two most widely used self-report burnout questionnaires: The Maslach Burnout Inventory (MBI) and the Burnout Measure (BM). Although the factorial validity of the three-dimensional structure of the MBI was convincingly demonstrated by confirmatory factor analysis with LISREL, four weak and ambiguous items were identified. Regarding the BM, some doubts have arisen about its one-dimensionality. The congruent validity of the questionnaires was well established: They both refer to the core element of the burnout syndrome (i.e. exhaustion). Moreover, linear structural analyses suggested that burnout is a two multi-dimensional construct consisting of an affective component (i.e. exhaustion) and an attitudinal component (i.e. a negative attitude towards recipients and towards one's job performance). However, the discriminant validity of the first component is rather poor since it considerably overlaps with self-reported somatic complaints and psychological strain. It is concluded that the MBI can be employed as a reliable and valid multi-dimensional indicator of burnout in professionals who work with people. The BM assesses the non-specific affective component of burnout (i.e. exhaustion) and should therefore be supplemented by a scale that measures the attitudinal component of the syndrome.     

Bacterioplankton Community Structure and Dynamics in Enclosures During Bioremediation Experiments in an Acid Mining Lake

In an acid mining lake (pH 2.6) enclosure experiments wereperformed with the addition of different concentrations oforganic carbon, nitrogen and phosphorus. SSCP-communityfingerprints, based on 16S rRNA gene amplicons, were performed tomonitor changes in the structure of the total bacterialcommunity and the sulfate reducing bacteria (SRB) in themesocosms. Total bacterial cell counts, as assessed byepifluorescence microscopy, were increased in the mesocosmsamended with organic carbon. The addition of carbon alsoincreased the number of abundant bacterial taxa substantiallyalong depth. Sulfate reducing bacteria (SRB) could be detected inall enclosures and all parts of the water column. These SRBbelonged to genus Desulfobacter as indicated bycoroborating molecular data.