Micronucleus induction by oxidative metabolites of trichloroethylene in cultured human peripheral blood lymphocytes: a comparative genotoxicity study

The genotoxic effects of oxidative metabolites of trichloroethylene (TCE), namely chloral hydrate, trichloroacetic acid (TCA), dichloroacetic acid (DCA), and trichloroethanol (TCEOH) were examined in human peripheral blood lymphocytes. In this context, lymphocytes were exposed in vitro to 25, 50, and 100 μg/ml concentrations of these metabolites separately for a period of 48 h and examined for micronucleus (MN) induction through flow cytometer. At 50 μg/ml TCE metabolites, TCA (6.33?±?0.56 %), DCA (5.06?±?0.55), and TCEOH (4.70?±?1.73) induced highly significant (p?<?0.001) frequency of MN in comparison to control (1.03?±?0.40) suggestive of their genotoxic potential. However, exposure of 100 μg/ml of all the metabolites consistently declined the frequencies of MN which in some cases was equable to that of observed at 25 μg/ml. Further, cytotoxicity and cell cycle disturbances were also measured to find out the association of these endpoints with the MN induction. DNA content analysis revealed 3–4-fold elevation of S-phase at all the concentrations tested. Particularly, at 100 μg/ml, treatment elevation of S-phase was significantly (p?<?0.0001) higher as compared to the control. Present findings together with earlier reports indicate that TCE induces genotoxicity through its metabolites. Interaction of these metabolites with DNA, as evident by elevated S-phase, seems to be the major cause of MN induction. However, involvement of spindle disruption cannot be ruled out. This comparative study also suggests that after TCE exposure, the metabolic efficiency of human to generate oxidative metabolites determines the extent of genotoxicity.     

Removal and recovery of uranium(VI) by adsorption onto a lignocellulosic-based polymeric adsorbent containing amidoxime chelating functional group

A new adsorbent (ABS) with amidoxime functional group was prepared through graft polymerization of acrylonitrile onto banana stem (BS) using ceric ammonium nitrate (CAN)/HNO₃ initiator system, followed by treatment with hydroxylamine hydrochloride in alkaline solution. Infrared spectroscopy, surface area analyzer, thermogravimetry, and potentiometric titration were used for the characterization of the adsorbent. Effective removal of U(VI) ions was demonstrated at the pH range 4.0–6.0. The mechanism for the removal of U(VI) ions by ABS was based on complexation adsorption model. Equilibrium was achieved in approximately 3 h. The experimental kinetic data were analyzed using first-order, second-order, and Elovich kinetic models, and are well fitted with second-order kinetics. The temperature dependence indicates an exothermic process. U(VI) adsorption was found to decrease with increase of ionic strength. The Freundlich isotherm model fitted the experimental equilibrium data well. The adsorption efficiency was tested using synthetic nuclear industry effluents. The maximum adsorption capacity for U(VI) removal was found to be 80 mg g^-1 at 20°C. Adsorbed U(VI) ions were desorbed effectively, about 99% by 0.2 M HCl. Repeated adsorption/desorption cycles show the feasibility of the ABS for the removal of U(VI) ions from water and nuclear industry effluents.     

Toxicity,degradation and analysis of the herbicide atrazine

Excessive use of pesticides and herbicides is a major environmental and health concern worldwide. Atrazine, a synthetic triazine herbicide commonly used to control grassy and broadleaf weeds in crops, is a major pollutant of soil and water ecosystems. Atrazine modifies the growth, enzymatic processes and photosynthesis in plants. Atrazine exerts mutagenicity, genotoxicity, defective cell division, erroneous lipid synthesis and hormonal imbalance in aquatic fauna and nontarget animals. It has threatened the sustainability of agricultural soils due to detrimental effects on resident soil microbial communities. The detection of atrazine in soil and reservoir sites is usually made by IR spectroscopy, ELISA, HPLC, UPLC, LC–MS and GC–MS techniques. HPLC/LC–MS and GC–MS techniques are considered the most effective tools, having detection limits up to ppb levels in different matrices. Biodegradation of atrazine by microbial species is increasingly being recognized as an eco-friendly, economically feasible and sustainable bioremediation strategy. This review presents the toxicity, analytical techniques, abiotic degradation and microbial metabolism of atrazine.     

Heavy metal speciation,leaching and toxicity status of a tropical rain-fed river Damodar,India

Speciations of metals were assessed in a tropical rain-fed river, flowing through the highly economically important part of the India. The pattern of distribution of heavy metals (Cd, Co, Cr, Cu, Mn, Ni, Pb and Zn) were evaluated in water and sediment along with mineralogical characterization, changes with different water quality parameters and their respective health hazard to the local population along the Damodar River basin during pre-monsoon and post-monsoon seasons. The outcome of the speciation analysis using MINTEQ indicated that free metal ions, carbonate, chloride and sulfate ions were predominantly in anionic inorganic fractions, while in cationic inorganic fractions metal loads were negligible. Metals loads were higher in sediment phase than in the aqueous phase. The estimated values of I_geo in river sediment during both the seasons showed that most of the metals were found in the I_geo class 0–1 which represents unpolluted to moderately polluted sediment status. The result of partition coefficient indicated the strong retention capability of Cr, Pb, Co and Mn, while Cd, Zn, Cu and Ni have resilient mobility capacity. The mineralogical analysis of sediment samples indicated that in Damodar River, quartz, kaolinite and calcite minerals were dominantly present. The hazard index values of Cd, Co and Cr were >?1 in river water, which suggested potential health risk for the children. A combination of pragmatic, computational and statistical relationship between ionic species and fractions of metals represented a strong persuasion for identifying the alikeness among the different sites of the river.     

Limnological investigations of Texi Temple pond in district Etawah (U.P.)

Present investigations were carried out on the limnological aspects of Texi temple pond in district Etawah. Many of the parameters were found below the permissible limits for drinking water as suggested by WHO. A total of 18 parameters were analysed and their seasonal variations in the year 2003 were discussed.     

Carbon footprint: current methods of estimation

Increasing greenhouse gaseous concentration in the atmosphere is perturbing the environment to cause grievous global warming and associated consequences. Following the rule that only measurable is manageable, mensuration of greenhouse gas intensiveness of different products, bodies, and processes is going on worldwide, expressed as their carbon footprints. The methodologies for carbon footprint calculations are still evolving and it is emerging as an important tool for greenhouse gas management. The concept of carbon footprinting has permeated and is being commercialized in all the areas of life and economy, but there is little coherence in definitions and calculations of carbon footprints among the studies. There are disagreements in the selection of gases, and the order of emissions to be covered in footprint calculations. Standards of greenhouse gas accounting are the common resources used in footprint calculations, although there is no mandatory provision of footprint verification. Carbon footprinting is intended to be a tool to guide the relevant emission cuts and verifications, its standardization at international level are therefore necessary. Present review describes the prevailing carbon footprinting methods and raises the related issues.     

Vulnerability of Indian mustard (<Emphasis Type="Italic">Brassica juncea</Emphasis> (L.) Czernj. Cosson) to climate variability and future adaptation strategies

A simulation study has been carried out using the InfoCrop mustard model to assess the impact of climate change and adaptation gains and to delineate the vulnerable regions for mustard (Brassica juncea (L.) Czernj. Cosson) production in India. On an all India basis, climate change is projected to reduce mustard grain yield by ～2 % in 2020 (2010–2039), ～7.9 % in 2050 (2040–2069) and ～15 % in 2080 (2070–2099) climate scenarios of MIROC3.2.HI (a global climate model) and Providing Regional Climates for Impact Studies (PRECIS, a regional climate model) models, if no adaptation is followed. However, spatiotemporal variations exist for the magnitude of impacts. Yield is projected to reduce in regions with current mean seasonal temperature regimes above 25/10 °C during crop growth. Adapting to climate change through a combination of improved input efficiency, additional fertilizers and adjusting the sowing time of current varieties can increase yield by ～17 %. With improved varieties, yield can be enhanced by ～25 % in 2020 climate scenario. But, projected benefits may reduce thereafter. Development of short-duration varieties and improved crop husbandry becomes essential for sustaining mustard yield in future climates. As climatically suitable period for mustard cultivation may reduce in future, short-duration (<130 days) cultivars with 63 % pod filling period will become more adaptable. There is a need to look beyond the suggested adaptation strategy to minimize the yield reduction in net vulnerable regions.     

Kinetic and equilibrium characterization of uranium(VI) adsorption onto carboxylate-functionalized poly(hydroxyethylmethacrylate)-grafted lignocellulosics

This study investigated the feasibility of using a new adsorbent prepared from coconut coir pith, CP (a coir industry-based lignocellulosic residue), for the removal of uranium [U(VI)] from aqueous solutions. The adsorbent (PGCP-COOH) having a carboxylate functional group at the chain end was synthesized by grafting poly(hydroxyethylmethacrylate) onto CP using potassium peroxydisulphate-sodium thiosulphite as a redox initiator and in the presence of N,N'-methylenebisacrylamide as a crosslinking agent. IR spectroscopy results confirm the graft copolymer formation and carboxylate functionalization. XRD studies confirm the decrease of crystallinity in PGCP-COOH compared to CP, and it favors the protrusion of the functional group into the aqueous medium. The thermal stability of the samples was studied using thermogravimetry (TG). Surface charge density of the samples as a function of pH was determined using potentiometric titration. The ability of PGCP-COOH to remove U(VI) from aqueous solutions was assessed using a batch adsorption technique. The maximum adsorption capacity was observed at the pH range 4.0-6.0. Maximum removal of 99.2% was observed for an initial concentration of 25mg/L at pH 6.0 and an adsorbent dose of 2g/L. Equilibrium was achieved in approximately 3h. The experimental kinetic data were analyzed using a first-order kinetic model. The temperature dependence indicates an endothermic process. U(VI) adsorption was found to decrease with an increase in ionic strength due to the formation of outer-sphere surface complexes on PGCP-COOH. Equilibrium data were best modeled by the Langmuir isotherm. The thermodynamic parameters such as DeltaG(0), DeltaH(0) and DeltaS(0) were derived to predict the nature of adsorption. Adsorption experiments were also conducted using a commercial cation exchanger, Ceralite IRC-50, with carboxylate functionality for comparison. Utility of the adsorbent was tested by removing U(VI) from simulated nuclear industry wastewater. Adsorbed U(VI) ions were desorbed effectively (about 96.2+/-3.3%) by 0.1M HCl. The adsorbent was suitable for repeated use (more than four cycles) without any noticeable loss of capacity.     

Correlation of edge truncation with antibacterial activity of plate-like anisotropic silver nanoparticles

Environmental Science and Pollution Research - The effect of silver nanoparticle anisotropy on the antibacterial properties has been studied against Escherichia coli, Staphylococcus aureus,...