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101.
Dollinger Jeanne Bourdat-Deschamps Marjolaine Pot Valérie Serre Valentin Bernet Nathalie Deslarue Ghislaine Montes Mélanie Capowiez Line Michel Eric 《Environmental science and pollution research international》2022,29(14):20098-20111
Environmental Science and Pollution Research - Organic waste (OW) reuse in agriculture is a common practice fostered by benefits in terms of waste recycling and crop production. However, OW... 相似文献
102.
Dromard Charlotte R. Allenou Jean-Pierre Tapie Nathalie Budzinski Hélène Cimmaterra Nicolas De Rock Pauline Arkam Salim Cordonnier Sébastien Gonzalez Jean-Louis Bouchon-Navaro Yolande Bouchon Claude Thouard Emmanuel 《Environmental science and pollution research international》2022,29(54):81546-81556
Environmental Science and Pollution Research - The present study, conducted in the Galion Bay in Martinique, aims to highlight the temporal and seasonal variations of chlordecone contamination (an... 相似文献
103.
At sites in fractured rock where contamination has been exposed to the rock matrix for extended periods of time, the amount of contaminant mass residing in the matrix can be considerable. Even though it may be possible to diminish concentrations by the advection of clean water through the fracture features, back diffusion from mass held in the matrix will lead to a continuing source of contamination. In such an event, the development of a biofilm (a thin film of microbial mass) on the wall of the fractures may act to limit or prevent the back diffusion process. The objective of this preliminary study is to explore the influence imparted by the presence of a biofilm on the process of matrix diffusion. The investigation was conducted using radial diffusion cells constructed from rock core in which biofilm growth was stimulated in a central reservoir. Once biofilms were developed, forward diffusion experiments were conducted in which a conservative solute migrated from the central reservoir into the intact rock sample. Diffusion experiments were performed in a total of 11 diffusion cell pairs where biofilm growth was stimulated in one member of the pair and inhibited in the other. The effect of the presence of a biofilm on tracer diffusion was determined by comparison of the diffusion curves produced by each cell pair. A semi-analytical model that accounts for the presence of a biofilm was used to investigate the effect of the biofilm on mass transfer due to changes in the effective porosity, effective diffusion coefficient, and the depth of penetration of the biofilm into the intact rock. The results show that the biofilm acted to plug the rock matrix, rather than forming a discrete layer on the reservoir surface. The reduction in effective porosity due to the biofilm ranged from 6% to 52% with the majority of the samples in the 30% to 50% range. Based on the present results, with more efficient biofilm stimulation, it is reasonable to assume that a more complete plugging of the microcrack porosity might be possible, leaving a much thicker and efficient barrier than could be achieved via a surface biofilm. 相似文献
104.
105.
Spinal muscular atrophy (SMA) preimplantation genetic diagnosis (PGD) has been available since 1998. Protocols are based on the detection of the homozygous deletion of exon 7, which are present in 90–98% of SMA patients. A couple where the woman was a heterozygous carrier of the usual SMN1 Del7 mutation and the man was a heterozygous carrier of pMet263Arg substitution in exon 6 of SMN1 gene was referred for PGD. The usual PGD test being unsuitable for this couple, we developed a novel duplex polymerase chain reaction (PCR)-based PGD test for the detection of the mutation pMet263Arg by allele specific amplification, combined with the amplification of D5S641 extragenic polymorphic marker. PCR conditions were established using single control lymphoblasts and lymphocytes from the pMet263Arg substitution carrier. Amplification was obtained in 100% of the 86 single cells tested, amplification refractory mutation system (ARMS) PCR was specific in 100% of single cells tested and a complete genotype (mutation plus D5S641) was achieved in 88% of them. A PGD cycle was performed successfully and a pregnancy was obtained. An unaffected girl was born and postnatal diagnosis confirmed PGD results. This is the first PGD described for SMA because of another mutation than the major homozygous exon 7 deletion of SMN1. In the future, a similar strategy could be adopted for other subtle mutations of this gene. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
106.
Allele-specific amplification for preimplantation genetic diagnosis (PGD) of spinal muscular atrophy
Céline Moutou Nathalie Gardes Catherine Rongières Jeanine Ohl Karima Bettahar-Lebugle Christiane Wittemer Pierre Gerlinger Stéphane Viville 《黑龙江环境通报》2001,21(6):498-503
We have developed a new allele-specific amplification method for the preimplantation genetic diagnosis (PGD) of spinal muscular atrophy (SMA; Werdnig-Hoffmann disease) from a single cell. This method is based on the detection of the deletion of exon 7 of the telomeric copy of the survival motor neurone (SMNt) gene. An oligonucleotide was designed to be specific to the SMNt nucleotidic sequence with exonic mismatch G (for SMNt)→A (for SMNc) at its 3′ end. This test produces reliable PCR products in 95% of single lymphoblasts (85/88) tested as well as in 16/16 blastomeres from normal controls. Specificity analysis showed that we were able to detect homozygous deletion of the SMNt gene in 99% of single lymphoblasts (103/104) from a SMA patient. No contamination was detected in 68 blanks tested. Multiple cell and DNA dilution analysis revealed that the test is accurate and specific up to 100 pg DNA and should thus also be suitable for PGD at the blastocyst stage. This rapid procedure requires a single round of fluorescent PCR and no restriction digestion, while previously described single cell methods include nested PCR followed by restriction enzyme digestion. Two PGD cycles for SMA using this procedure were performed in our centre. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
107.
Morgane Roger Nathalie Bonnardel Ludovic Le Bigot 《Journal of environmental psychology》2011,31(2):192-199
Tools for assisting human navigation, especially in-vehicle systems, have been extensively investigated. However, few studies have explored the design of speech-based over-the-phone guidance systems. This study examined the effect of landmarks' use and the effect of landmarks’ frames of reference in route instructions on navigation efficiency during map navigation tasks and satisfaction. Twenty-seven participants performed map navigation tasks using a simulated speech navigation system in three experimental conditions: instructions containing no landmarks, instructions containing landmarks located without reference to either the traveler’s body or the surrounding environment, and instructions containing landmarks located with respect to the traveler’s body. Navigation performances on maps were higher and landmarks enable participants to make fewer directional errors and find their routes more efficiently. Satisfaction levels and navigation performances were lower when instructions did not contain any landmarks. Landmarks’ frames of reference shifting turned out to be different between human-human situations previously used and human-computer situations used here. 相似文献
108.
Identification of flavonoids in Pinus
Laricio needles and changes occurring after prescribed burning
Magali Cannac Lila Ferrat Toussaint Barboni Nathalie Chiaramonti Fr��d��ric Morandini Vanina Pasqualini 《Chemoecology》2011,21(1):9-17
Prescribed burning is increasingly being used as a fire prevention tool in forest management; however, it can cause thermal
stress to trees. Its effects on pine physiology have been poorly understood. Flavonoids are highly indicative of plants’ responses
to environmental stress, and in this study, we used flavonoid concentrations in pine needles to assess fire impact on a Corsican
Pinus
laricio Poir. forest up to 4 years after prescribed burning. This is the first study that highlighted the presence of flavonols in
P. laricio and indicated kaempferol to be the major compound. The total flavonol content increased by 60% 3 months after 1 or 2 prescribed
low-intensity burnings and returned to the pre-burning level after 1 year. This suggests that thermal stress events caused
defensive production of flavonoids. Prescribed burnings performed at 3-year intervals did not appear to alter flavonoid levels.
In the light of these findings, forest managers may consider the use of prescribed burning at 3-year intervals as a means
to reduce fuel loads, while minimizing the impact on P.
laricio. 相似文献
109.
A comparative Life Cycle Assessment (LCA) of solar photo-Fenton and solar photoelectro-Fenton, two solar-driven advanced oxidation processes (AOPs) devoted to the removal of non-biodegradable pollutants in water, is performed. The study is based on the removal, at laboratory scale, of the amino acid α-methylphenylglycine, a good example of soluble and non-biodegradable target pollutant. The system under study includes chemicals, electricity, transport of all raw materials to the plant site, and the generation of emissions, but it does not take into account the impact of the infrastructure needed to build a hypothetical solar plant. Nine environmental impact categories are included in the LCA: global warming potential, ozone depletion potential, aquatic eutrophication potential, acidification potential, human toxicity potential, photochemical ozone formation potential, fresh water aquatic ecotoxicity potential, marine aquatic ecotoxicity potential, and terrestrial ecotoxicity potential and abiotic resource depletion potential. Although previous experimental results show that both AOPs are able to efficiently degrade the pollutant, the LCA indicates that solar-driven photo-Fenton is the most environmentally friendly alternative, mainly because the use of electricity in solar photoelectro-Fenton experiments involves high environmental impacts. 相似文献
110.
Gihad E. Chalouhi Anne-Élodie Millischer Houman Mahallati Nathalie Siauve Andrew Melbourne David Grevent Nicolas Vinit Laurence Heidet Yves Aigrain Yves Ville Thomas Blanc Laurent J. Salomon 《黑龙江环境通报》2020,40(1):100-109
Fetal anomalies are detected in approximately 2% of all fetuses and, among these, genitourinary tract abnormalities account for 30% to 50% of all structural anomalies present at birth. Although ultrasound remains the first line diagnostic modality, fetal MRI provides important additional structural and functional information, especially with the development of faster sequences and the use of functional sequences. The added value of MRI-based imaging is three-fold: (a) improvement of diagnostic accuracy by adequate morphological examination, (b) detection of additional anomalies, and (c) in addition, MRI has the potential to provide information regarding renal function. In this review, we describe the role of fetal MRI in the anatomical evaluation of renal and urogenital tract anomalies, and we also touch upon the contribution of functional MRI to the diagnostic workup of these conditions. 相似文献