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971.
972.
973.
974.
Genetic structure of fissiparous populations of Holothuria (Halodeima) atra on the Great Barrier Reef 总被引:1,自引:0,他引:1
Allozyme variation at five polymorphic loci was surveyed in a total of 311 individuals of the sea cucumber Holothuria (Halodeima) atra (Jäger, 1833) collected from two nearshore and two midshelf populations in the Great Barrier Reef in November 1996. Strong deviations in genotype frequencies from those expected under Hardy–Weinberg equilibrium, particularly a large number of heterozygote excesses, confirmed the occurrence of asexual reproduction. Females and males differed significantly in genotype frequencies as a result of differences in either the amount of fission in each sex or in the mortality of asexually produced recruits. The estimated maximum sexual input (number of sexually produced individuals: sample size = N*:Ni) to the two nearshore reefs (38 to 67%) was low relative to that to the two midshelf reefs (74 to 87%). The three ratios and G o :G e , N go :N i , N*:N i , (where G o = observed genotypic diversity, G e = expected genotypic diversity, N go = number of genotypes) considered to be indicators of the extent of asexual reproduction, showed a consistent trend in the degree of asexual reproduction similar to that derived from the number of regenerating individuals observed in the populations for which data were available. F-statistic analyses of clonal gene frequencies demonstrated that all populations received sexual recruits from the same gene pool. There was evidence of restricted sexual recruitment to the Fantome population, suggesting that asexual reproduction was dominant only in areas where sexual recruitment was limited by other factors. 相似文献
975.
We investigated mycosporine amino acid (MAA) involvement as protective sunscreens in spawn of the sea hare Aplysia dactylomela to determine if adult diet and ultraviolet (UV) exposure affected the UV sensitivity of developing embryos. Adults were fed
a red alga rich in MAAs (Acanthophora spicifera) or a green alga poor in MAAs (Ulva lactuca). Adults on each diet were exposed for 2 wk to ambient solar irradiance with two types of acrylic filters; one allowed exposure
to wavelengths >275 nm (designated UV) and one to wavelengths only >410 nm (designated NOUV). Spawn from each adult group
was likewise treated with UV or NOUV and monitored during development for differences in mortality and metabolic rate (measured
as oxygen consumption: V˙
O2). Also recorded were number of eggs or embryos per capsule, times to hatching, hatching success, size at hatching, and V˙
O2 of adults. Spawn from adults eating red algae was almost twice as rich in MAAs as spawn from adults eating green algae, suggesting
that MAA content is diet-related. Although overall quantities of MAAs in the spawn reflected MAA contents of the adult diet,
specific MAAs were differentially sequestered in the spawn. Thus, porphyra-334, found in high concentration in Aplysia dactylomela's preferred red algal food, was present in only low concentration in the spawn. Conversely, mycosporine-glycine, in low concentration
in red algal food, was the most abundant MAA in the spawn. UV treatment of adults had no effect on quantities of MAAs in the
spawn. Adults exposed to UV had significantly higher V˙
O2s and spawned twice as often. The UV-treated adults produced spawn with significantly higher V˙
O2s and their embryos developed to hatching sooner. The only significant effect of UV exposure of the spawn was to reduce the
percentage of veligers hatching from 71 to 50%. There was no significant effect on hatching time or size of the veligers at
hatching, nor on number of eggs per capsule.
Received: 13 May 1997 / Accepted: 27 June 1997 相似文献
976.
Effects of specimen handling and otolith preparation on concentration of elements in fish otoliths 总被引:2,自引:0,他引:2
The use of otolith chemistry as a tool for analysing the biology of fishes assumes that the procedures used to collect and
prepare otoliths for analysis do not alter their composition. With otoliths of Nemadactylus macropterus, Hoplostethus atlanticus, and Rhombosolea tapirina, we show that this assumption is not valid for the elements that can be detected using electron-probe microanalysers (those
present at concentrations greater than ≃100 parts per million): all six elements routinely measured using these techniques
were affected by at least one post-mortem procedure tested. Measured concentrations of calcium and strontium were relatively
insensitive to most procedures tested, whereas concentrations of sodium, potassium, sulphur and chlorine were affected substantially
by many commonly used procedures. The ease with which otolith composition could be modified post-mortem suggests that apparent
geographic, habitat-specific or ontogenetic differences in otolith composition should be interpreted with extreme caution
because of easily induced artefacts and the problem of pseudo-replication associated with the ways otoliths are sampled and
prepared for analysis.
Received: 23 September 1996 / Accepted: 11 April 1998 相似文献
977.
Genetic divergence and phylogenetic inferences in five species of Mugilidae (Pisces: Perciformes) 总被引:4,自引:0,他引:4
Allozyme electrophoresis was used to compare the genetic divergence of global populations of Mugil cephalus Linnaeus, 1758 and two congeneric [M.␣curema Cuvier & Valenciennes, 1836; M. gyrans (Jordan & Gilbert, 1885)] and two more distantly related [Liza ramada (Risso, 1826); Xenomugil thoburni (Jordan & Starks, 1896)] species on the basis of 18 enzyme loci. The amount of genetic divergence among the species examined
is in agreement with their present systematic status, the differences being larger among non-congeneric species than among
species of the same genus. Intraspecific genetic distances in M. cephalus (average Nei's D= 0.154), although higher than those reported for conspecific populations of fish, appear to be small when compared to the
interspecific values among mugilid species (0.821 ≤ Nei's D ≤ 1.744). Phylogenetic trees obtained by genetic distance methods and discrete character parsimony analysis were of similar
topology, except for the relationships within the genus Mugil and for the arrangement of M. cephalus populations.
Received: 7 April 1997 / Accepted: 4 February 1998 相似文献
978.
Queen control of egg fertilization in the honey bee 总被引:3,自引:0,他引:3
The study investigated the precision with which honey bee queens can control the fertilization of the eggs they lay. Because
males and workers are reared in different-sized cells, the honey bee is one of the few Hymenoptera in which it is possible
for the experimenter to know which type of egg a queen “intends” to lay. Eggs were collected from both worker and drone (male)
cells from four honey bee colonies. Ploidy of the embryo was determined using polymorphic DNA microsatellites. All 169 eggs
taken from worker cells were heterozygous at at least one microsatellite locus showing that the egg was fertilized. All 129
eggs taken from drone cells gave a single band at the B124 locus, strongly suggesting haploidy. These data show that honey
bee queens have great, and quite possibly complete, ability to control the fertilization of the eggs they lay. Data from the
literature suggest that in two species of parasitoid Hymenoptera (Copidosoma floridanum, Colpoclypeus florus) females have great, but not complete, ability to control fertilization.
Received: 23 December 1997 / Accepted after revision: 17 May 1998 相似文献
979.
980.