Measurement of contaminant removal from skin using a portable fluorescence scanning system

The residence time of particulate contamination on the human body is a factor that has an important impact on the accuracy of exposure assessment in the aftermath of an accidental release of radionuclides to the atmosphere. Measurements of particle clearance from human skin were made using an illumination system to excite fluorescence in labelled silica particles and a CCD camera and image processing system to detect this fluorescence. The illumination system consists of high-intensity light emitting diodes (LEDS) of suitable wavelengths arranged on a portable stand. The physically small size of the LEDs allows them to be positioned close to the fluorescing surface, thus maximising the fluorescent signal that can be obtained. The limit of detection was found to be 50 microg of tracer particle per cm2. Experiments were carried out to determine the clearance rates of 10 microm and 3 microm particles from the skin. Results show that, in the absence of any mechanical rubbing of the skin, the clearance of particles from the skin followed an approximately exponential decay with a half-time of 1.5-7.8 h. Skin hairiness and degree of human movement were found, in addition to particle size, to have an important influence on particle fall-off rate.     

Different patterns of carotenoid composition and photosynthesis acclimation in two tropical red algae

To be able to survive, marine macroalgae in shallow coastal waters need mechanisms for short-term acclimation to fast changes in their environment. Of major importance are mechanisms that regulate the efficiency of photosynthesis by protecting PS II from photo-oxidative damage. Carotenoids, xanthophyll cycles and non-photochemical quenching (NPQ) are central constituents of such protection mechanisms. Red algae as a group do not have a universal carotenoid composition. We screened ten red algal species and selected two species, originating from similar ecological conditions but with different carotenoid compositions, for use in irradiance-acclimation experiments. We selected the tropical intertidal species Gracilaria domingensis and Kappaphycus alvarezii with antheraxanthin and lutein as major xanthophylls, respectively. Simultaneous in vivo fluorescence and O₂ evolution experiments were performed at different irradiance levels, which allowed a direct comparison of overall photosynthetic performance with NPQ. Interconversions of xanthophylls (violaxanthin, zeaxanthin, β-cryptoxanthin and one unidentified carotenoid) did occur in G. domingensis, but not in response to sudden exposure to light. Thus, NPQ was not correlated with any xanthophyll cycle during short-term acclimation to light. G. domingensis had five times higher weight-specific photosynthetic rates than K. alvarezii, which can be explained by the thicker thallus of K. alvarezii. Chlorophyll-specific gross photosynthetic rates were higher in K. alvarezii, but net rates were the same for both species. G. domingensis showed an immediate strong onset of NPQ upon exposure to irradiance, followed by downregulation to the NPQ level required. In K. alvarezii NPQ increased slowly until the required NPQ level was reached. At high irradiance G. domingensis downregulated photosynthesis while K. alvarezii continued to produce O₂ even at 2,000 μmol photons m⁻² s⁻¹ without NPQ increase. The strategy of K. alvarezii may provide short-term gains but with the risk of oxidative damage. The fast onset of NPQ in G. domingensis even at subsaturating irradiance as well as downregulation of photosynthesis when NPQ is saturated might provide this species with a competitive advantage under conditions of changing irradiance in the field.     

Energy use in Denmark

This paper reviews the evolution of energy use in Denmark since the early 1970s, in order to shed light on the future path of energy use in Denmark, with particular emphasis on the role of energy efficiency. Improvements in end-use energy efficiency reduced primary energy requirements in Denmark by 22% between 1972 and 1988. This change accounts for two-thirds of the decline in the ratio of energy use to gross domestic product that occurred during this time: the rest of the decline was caused by changes in the mix of goods and services produced and consumed by the Danes. Total energy savings achieved between 1972 and 1988 in Denmark ranked among the highest measured in any major OECD country. Overall, most of the energy savings in Denmark were brought about through improvements in technology. Short-term changes in consumer behaviour were significant in reducing energy needs for space heating and transport. An important stimulus for improved efficiency was higher energy prices, led in no small part by significant taxes imposed on small consumers of heating oil, electricity and motor fuels.     

Micromechanical Tensile Testing of Cellulose-Reinforced Electrospun Fibers Using a Template Transfer Method (TTM)

A template transfer method (TTM) and a fiber fixation technique were established for fiber handling and micro tensile stage mounting of aligned and non-aligned electrospun fiber mats. The custom-made template had been precut to be mounted on a variety of collectors, including a rapidly rotating collector used to align the fibers. The method eliminated need for direct physical interaction with the fiber mats before or during the tensile testing since the fiber mats were never directly clamped or removed from the original substrate. By using the TTM it was possible to measure the tensile properties of aligned poly(methyl methacrylate) (PMMA) fiber mats, which showed a 250?% increase in strength and 450?% increase in modulus as compared to a non-aligned system. The method was further evaluated for aligned PMMA fibers reinforced with cellulose (4 wt%) prepared as enzymatically derived nanofibrillated cellulose (NFC). These fibers showed an additional increase of 30?% in both tensile strength and modulus, resulting in a toughness increase of 25?%. The fracture interfaces of the PMMA?CNFC fibers showed a low amount of NFC pull-outs, indicating favorable phase compatibility. The presented fiber handling technique is universal and may be applied where conservative estimates of mechanical properties need to be assessed for very thin fibers.     

Entrepreneurship in climate governance at the local and regional levels: concepts,methods, patterns,and effects

This editorial sets the scene for a special issue on climate governance entrepreneurship at the local and regional levels. To make climate governance work, much policy activity is needed at the local and regional levels. Entrepreneurs are actors who aim to affect change by using their agency. They target policy decisions at the local and regional levels, which might subsequently turn to other governance levels to expand their influence. The scientific discussion about governance entrepreneurs is characterized by a lack of conceptual clarity, by methodological challenges, and by several research gaps. Regarding the latter, at present, it is especially unclear when and why entrepreneurs become active, which factors they take into account when they select their strategies, and what explains the effects of entrepreneurial activity on the emergence of innovations in climate governance. All contributions to this special issue engage with one or several of these conceptual, methodological, and empirical challenges, thus advancing the state of art in the field. Highlights from the special issue include the development of a simple conceptual frame that connects actors, contexts, strategies, and outcomes in a systematic way. Some promising methodological avenues are described, since the special issue contains not only some qualitative case studies but also some studies that take a long-term perspective by following policy development for decades, and a study that proposes a census approach. Empirically, the contributions in this special issue shed light on a range of factors explaining levels of entrepreneurial activity, and they carefully trace impacts over time. We conclude by sketching an agenda for further work in this realm.

     

Non-consumptive effects of predator presence on copepod reproduction: insights from a mesocosm experiment

Reproduction in planktonic animals depends on numerous biotic and abiotic factors. One of them is predation pressure, which can have both direct consumptive effects on population density and sex ratio, and non-consumptive effects, for example on mating and migration behaviour. In copepods, predator vulnerability depends on their sex, motility pattern and mating behaviour. Therefore, copepods can be affected at multiple stages during the mating process. We investigated the reproductive dynamics of the estuarine copepod Eurytemora affinis in the presence and absence of its predator the mysid Neomysis integer in a mesocosm experiment. We found that the proportion of ovigerous females decreased in the presence of predators. This shift was not caused by differential predation as the absolute number of females was unaffected by mysid presence. Presence of predators reduced the ratio of males to non-ovigerous females, but not by predation of males. Our combined results suggest that the shift from ovigerous to non-ovigerous females under the presence of predators was caused by either actively delayed egg production or by shedding of egg sacs. Nauplii production was initially suppressed in the predation treatment, but increased towards the end of the experiment. The proportion of fertilized females was similar in both treatments, but constantly fell behind model predictions using a random mating model. Our results highlight the importance of non-consumptive effects of predators on copepod reproduction and hence on population dynamics.     

Environmental problems and environmental regulations in Western Europe, 1980–1989

Environmental problems are a function of the process of economic development and the ability to mitigate the problems through regulation. An historical analysis helps to show how the problems have been solved thus far. The objective of this investigation is to present an overview of environmental problems and the corresponding regulations in Western Europe during the 1980s. Environmental problems for 18 Western European countries were identified from abstracts from a data base on environmental literature. The 1977 findings were systematized on the basis of 20 key words according to source and impact, and a new data base was established. The data showed that the main environmental problems in Western Europe were: energy production/acid rain, nuclear power/waste, air pollution/climate, heavy metals, pesticides, water pollution, solid waste, and unforeseen accidents. Many environmental problems are common to most European countries. Regulatory measures differ significantly. In some countries environmental regulations are largely limited to the implementation of European Economic Community (EEC) directives. We have supplemented the national analyses with an examination of existing and proposed EEC regulations along with interviews with civil servants in the EEC Commission. In the 1980s, EEC environmental regulations were similar to environmental regulations in some member states. It is expected that future EEC regulations will be less reflective of those of individual member states.     

In situ tracer tests to determine retention properties of a block scale fracture network in granitic rock at the Aspö Hard Rock Laboratory, Sweden

Experiments were conducted at the Asp? Hard Rock Laboratory in order to improve the understanding of radionuclide retention properties of fractured crystalline bedrock in the 10-100 m scale (TRUE Block Scale Project, jointly funded by ANDRA, ENRESA, Nirex, JNC, Posiva and SKB). A series of tracer experiments were performed using sorbing tracers in three different flow paths. The different flow paths had Euclidian lengths of 14, 17 and 33 m, respectively, and one to three water conducting structures. Four tests were performed using different cocktails made up of radioactive sorbing tracers (22,24Na+, 42K+, 47Ca2+, 85Sr2+, 83,86Rb+, 131,133Ba2+ and 134,137Cs+). For each tracer injection, the breakthrough of sorbing tracers was compared to the breakthrough of a conservative tracer, 82Br-, 131I-, HTO and 186ReO4-, respectively. In the two longer flow paths, no breakthrough of 83Rb+ and 137Cs+ was observed after 8 months of pumping. Selected tracer tests were subject to basic modelling in which a one-dimensional (1D) advection-dispersion model, including surface sorption, and an unlimited matrix diffusion were used for the interpretation of the results. The results of the modelling indicated that there is a slightly higher mass transfer into a highly porous material in the block-scale experiment compared with in situ experiments performed over shorter distances and significantly higher than what would have been expected from laboratory data obtained from studies of the interactions in nonaltered intact rock.     

Protein futures for Western Europe: potential land use and climate impacts in 2050

Multiple production and demand side measures are needed to improve food system sustainability. This study quantified the theoretical minimum agricultural land requirements to supply Western Europe with food in 2050 from its own land base, together with GHG emissions arising. Assuming that crop yield gaps in agriculture are closed, livestock production efficiencies increased and waste at all stages reduced, a range of food consumption scenarios were modelled each based on different ‘protein futures’. The scenarios were as follows: intensive and efficient livestock production using today’s species mix; intensive efficient poultry–dairy production; intensive efficient aquaculture–dairy; artificial meat and dairy; livestock on ‘ecological leftovers’ (livestock reared only on land unsuited to cropping, agricultural residues and food waste, with consumption capped at that level of availability); and a ‘plant-based eating’ scenario. For each scenario, ‘projected diet’ and ‘healthy diet’ variants were modelled. Finally, we quantified the theoretical maximum carbon sequestration potential from afforestation of spared agricultural land. Results indicate that land use could be cut by 14–86 % and GHG emissions reduced by up to approximately 90 %. The yearly carbon storage potential arising from spared agricultural land ranged from 90 to 700 Mt CO₂ in 2050. The artificial meat and plant-based scenarios achieved the greatest land use and GHG reductions and the greatest carbon sequestration potential. The ‘ecological leftover’ scenario required the least cropland as compared with the other meat-containing scenarios, but all available pasture was used, and GHG emissions were higher if meat consumption was not capped at healthy levels.