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101.
Prata C Ribeiro A Cunha  Gomes NC Almeida A 《Journal of environmental monitoring : JEM》2012,14(1):64-70
Some health important enteric viruses are considered to be emerging waterborne pathogens and so the improvement of detection of these viruses in the aquatic environment is one of the most important steps in dealing with these pathogens. Since these viruses may be present in low numbers in water, it is necessary to concentrate water samples before viral detection. Although there are several methods to concentrate viruses in environmental waters, all present some drawbacks and consequently the method should be chosen that, despite its limitations, is adequate to achieve the aim of each study. As the effectiveness of the concentration methods is evaluated by determining the efficiency of viral recovery after concentration, it is important to use a simple and effective approach to evaluate their recovery efficiency. In this work ultracentrifugation, usually used as a secondary step for virus concentration, was evaluated as the main method to concentrate directly viruses in environmental water samples, using the microscopic enumeration of virus-like particles (VLP) as a new approach to estimate the efficiency of recovery. As the flocculation method is currently employed to concentrate viruses in environmental waters, it was also used in this study to assess the efficiency of the ultracentrifugation as the main viral concentration method in environmental waters. The results of this study indicate that ultracentrifugation is an adequate approach to concentrate viruses directly from environmental waters (recovery percentages between 66 and 72% in wastewaters and between 66 and 76% in recreational waters) and that the determination of VLP by epifluorescence microscopy is a simple, fast and cheap alternative approach to determine the recovery efficiency of the viral concentration methods. 相似文献
102.
103.
A Systematic Framework for the Design and Implementation of a Quality Management Practice: The Case of a Consulting Engineering Company
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Herlander Mata‐Lima Fernando Morgado‐Dias Marina Carrato Galuzzi da Silva Kelly Alcântara José António Almeida 《环境质量管理》2016,25(4):49-61
Company productivity and competitiveness in today's marketplace requires creative design, innovation, and the reduction of nonconformances, which require costly rework and can damage a firm's reputation with its customers and potential customers. Using the International Organisation of Standardization's (ISO) 9000 and 14000 series of standards, the authors of this article walk readers through the rigorous process of obtaining upper management buy‐in and sponsorship and engaging workers in a bottom‐up approach to establish the procedures necessary to perform world‐class engineering services. 相似文献
104.
Valéria R. F. da Silva Sylvia F. Mitraud Maria L. C. P. Ferraz Eduardo H. S. M. Lima Maria Thereza D. Melo Armando J. B. Santos Augusto César C. D. da Silva Jaqueline C. de Castilhos Jamyle A. F. Batista Gustave G. Lopez Frederico Tognin João Carlos Thomé Cecília Baptistotte Berenice M. Gomes da Silva José Henrique Becker Juçara Wanderline Fernanda de Vasconcellos Pegas Gonzalo Róstan Guy Guagni dei Marcovaldi Maria Ângela G. dei Marcovaldi 《Environment, Development and Sustainability》2016,18(6):1541-1558
In the 35 years since its inception, the Brazilian National Program for the Conservation of Marine Turtles (TAMAR) has had great success in protecting the five species of sea turtles that occur in Brazil. It has also contributed significantly to worldwide scientific data and knowledge about these species’ biology, such as life cycles and migration patterns. TAMAR’s conservation strategies have always relied on a variety of environmental education and social inclusion (EESI) activities highly adapted to the socio-environmental evolving contexts of its 25 locations distributed across nine states. Diversity and flexibility are critical to enable timely and effective local responses to existing or potential threats to sea turtles. The intuitive, locally adapted, decentralized, and independent way EESI activities have been carried out have generated positive results in the resolution of specific and evolving local problems through the course of the project. This article brings EESI under the same conceptual framework that underlies its conservation approach by adopting an adaptive threat management framework to organize and qualify its educational and social inclusion interventions according to the main categories of threat addressed by TAMAR. 相似文献
105.
Dalel Daâssi Lassaad Belbahri Armelle Vallat Steve Woodward Moncef Nasri Tahar Mechichi 《Environmental science and pollution research international》2014,21(3):1746-1758
The search for novel microorganisms able to degrade olive mill wastewaters (OMW) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. In this work, the possibility of reducing the phenolic content of OMW using new isolates of fungal strains (Coriolopsis gallica, Bjerkandera adusta, Trametes versicolor, Trichoderma citrinoviride, Phanerochaete chrysosporium, Gloeophyllum trabeum, Trametes trogii, and Fusarium solani) was investigated. In vitro, all fungal isolates tested caused an outstanding decolorization of OMW. However, C. gallica gave the highest decolorization and dephenolization rates at 30 % v/v OMW dilution in water. Fungal growth in OMW medium was affected by several parameters including phenolic compound concentration, nitrogen source, and inoculum size. The optimal OMW medium for the removal of phenolics and color was with the OMW concentration (in percent)/[(NH4)2SO4]/inoculum ratio of 30:6:3. Under these conditions, 90 and 85 % of the initial phenolic compounds and color were removed, respectively. High-pressure liquid chromatography analysis of extracts from treated and untreated OMW showed a clear and substantial reduction in phenolic compound concentrations. Phytotoxicity, assessed using radish (Raphanus sativus) seeds, indicated an increase in germination index of 23–92 % when a 30 % OMW concentration was treated with C. gallica in different dilutions (1/2, 1/4, and 1/8). Figure
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106.
Crestani M Menezes C Glusczak L dos Santos Miron D Spanevello R Silveira A Gonçalves FF Zanella R Loro VL 《Chemosphere》2007,67(11):2305-2311
The effects of the herbicide, clomazone, on acetylcholinesterase (AChE), catalase and TBARS formation in teleost fish (Rhamdia quelen) were studied. The fish were exposed to 0.5 or 1.0 mg L−1 of clomazone for 12, 24, 48, 96 and 192 h. After 192 h of exposure period, fish were transferred to clean water and kept in the same for 192 h to study the recovery response. Same parameters as that of exposure period were assayed after 96 and 192 h of recovery period. Specific AChE activity was reduced in the brain and muscle after treatments, reaching a maximum inhibition of 47% in the brain and 45% in the muscle after 12 h of exposure. Fish exposed to clomazone increased TBARS production in the liver for all exposure periods. The brain presented elevated TBARS levels after 12, 24 and 48 h, but after 96 and 192 h, these levels decreased. The decrease of TBARS levels persisted in brain tissue after 96 h of recovery and returned to the control value after 192 h in clean water. Catalase activity was reduced for all periods of exposure. Histological analysis showed vacuolation in the liver after herbicide exposure. Some of the alterations observed were completely restored after recovery period. 相似文献
107.
Koubaa Fatma Ghorbel Chaâbane Mariem Turki Mouna Ayadi Fatma Makni El Feki Abdelfattah 《Environmental science and pollution research international》2021,28(9):11001-11015
Environmental Science and Pollution Research - The present study was designed to evaluate the protective effects of Salvia officinalis essential oil (SOEO) against vanadium-induced hepatotoxicity... 相似文献
108.
Le Conte Y Bécard JM Costagliola G de Vaublanc G El Maâtaoui M Crauser D Plettner E Slessor KN 《Die Naturwissenschaften》2006,93(5):237-241
A brood pheromone identified in honeybee larvae has primer and releaser pheromone effects on adult bees. Using gas chromatography–mass spectrometry (GC–MS) to evaluate fatty acid esters—the pheromonal compounds—in different parts of the larvae, we have localized the source of the esters as the larval salivary glands. A histochemical study describes the glands and confirms the presence of lipids in the glands. Epithelial cells of the gland likely secrete the fatty acids into the lumen of the gland. These results demonstrate the salivary glands to be a reservoir of esters, components of brood pheromone, in honeybee larvae. 相似文献
109.
Rissato SR Galhiane MS Ximenes VF de Andrade RM Talamoni JL Libânio M de Almeida MV Apon BM Cavalari AA 《Chemosphere》2006,65(11):1949-1958
Detailed analyses of persistent organic pollutants (POPs) such as organochlorine pesticides (OCPs), hexachlorocyclohexane (HCH) isomers (HCHs), dichlorodiphenyltrichloro ethane (DDT) and its metabolites (DDTs) and congeners of polychlorinated biphenyls (PCBs) in soil and surface water from the northeastern São Paulo, Brazil allowed the evaluation of the contamination status, distribution and possible pollution sources. The pesticides and PCBs demonstrated markedly different distributions, reflecting different agricultural, domestic and industrial usage in each region studied. The ranges of HCH, DDT, and PCBs concentrations in the soil samples were 0.05–0.92, 0.12–11.01, 0.02–0.25 ng g−1 dry wt, respectively, and in the surface water samples were 0.02–0.6, 0.02–0.58 and 0.02–0.5 ng l−1, respectively. Overall elevated levels of DDT and PCB were recorded in region 2, a site very close to melting, automotive batteries industries, and agricultural practice regions. High ratios of metabolites of DDT to DDT isomers revealed the recent use of DDT in this environment. The sources of contamination are closely related to human activities, such as domestic and industrial discharge, street runoff, agricultural pesticides and soil erosion, due to deforestation as well as atmospheric transport. 相似文献