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Grebe and seaduck species were collected during late winter at industrial and reference sites along coastal British Columbia, and during spring migration in the Yukon Territory, from 1988 to 1993. Liver and/or breast muscle were analyzed for polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), biphenyls (PCBs), organochlorine (OC) pesticides, and chlorophenol-related compounds. Piscivorous species, including western grebes (Aechmophorus occidentalis) and common mergansers (Mergus merganser), contained highest levels of all contaminants. DDE and PCBs were detectable in all species analyzed, but were at low levels (<50 and 100 g/kg [sum PCBs] wet weight, respectively) in all but some grebe and merganser samples. Highest DDE concentration (229 g/kg) was in liver of common mergansers collected at Port Alberni in 1989, and that of PCB (2300 g/kg) in breast muscle of western grebes collected in 1992 from the same site. The interspecific PCDD/PCDF pattern was similar to that of the OC pesticides and PCBs, with the fish-eating species containing highest concentrations. The only congener detected in all samples was 2,3,7,8-TCDF, although 2,3,7,8-TCDD was regularly present. Generally, of all samples collected in 1989, those from the bleached-kraft pulp mill (BKPM) site at Port Alberni were the most contaminated with PCDDs and PCDFs. Of the chlorophenolic compounds measured, only pentachlorophenol was routinely detected, typically at levels below 5 g/kg. Traces of 3,4,5,6-tetrachloroguaiacol, 5-chloroguaiacol and 4,5-dichloroguaiacol were also detected in a few samples, mainly from a BKPM site at Prince Rupert. By 1992, after changes to pulp mill bleaching processes and restrictions in chlorophenolic anti-sapstain use, PCDD and PCDF concentrations were substantially lower compared to 1989 in all species sampled and no longer posed a concern for human consumers. International TCDD-toxic equivalents (I-TEQs) in some western grebe samples were within the range of concentrations associated with sublethal effects in waterbird species (200–400 ng/kg). 相似文献
43.
J. G. Elliott R. S. Parker 《Journal of the American Water Resources Association》1997,33(5):1041-1054
ABSTRACT: The Gunnison River in the Gunnison Gorge is a canyon river where upstream dams regulate mainstem discharge but do not affect debris-flow sediment supply from tributaries entering below the reservoirs. Regulation since 1966 has altered flood frequency, streambed mobility, and fluvial geomorphology creating potential resource-management issues. The duration of moderate streamflows between 32.3 and 85.0 m3/s has increased threefold since 1966. This, along with flood-peak attenuation, has facilitated fine-sediment deposition and vegetation encroachment on stream banks. The Shields equation and on-site channel geometry and bed-material measurements were used to assess changes in sediment entrainment in four alluvial reaches. Sand and fine gravel are transported through riffle/pool reaches at most discharges, but the cobbles and boulders composing the streambed in many reaches now are infrequently entrained. Periodic debris flows add coarse sediment to rapids and can increase pool elevation and the streambed area affected by backwater and fine-sediment accumulation. Debris-flow supplied boulders accumulate on fans and in rapids and constrict the channel until reworked by larger floods. The response to streamflow-régime changes in the Gunnison Gorge could serve as an analog for alluvial reaches in other regulated canyon rivers. 相似文献
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Biacore biosensor immunoassay for 4-nonylphenols: assay optimization and applicability for shellfish analysis 总被引:4,自引:0,他引:4
A rapid Biacore biosensor immunoassay of 4-nonylphenols was developed. Two types of antibodies were used in the study: polyclonal antibodies with high cross-reactivity towards technical 4-nonylphenol and a monoclonal antibody very specific to 4-n-nonylphenol. 9-(p-Hydroxyphenyl)nonanoic acid was immobilized onto surface of a sensor chip. The best assay sensitivity was achieved using a flow rate of 50 microl min(-1) and injection time of 2 min. For the assay incorporating monoclonal antibodies a limit of detection 2 ng ml(-1) for 4-n-nonylphenol was achieved. With polyclonal antibodies one order lower sensitivity was observed for 4-nonylphenols. High background level of calibration curve for technical 4-nonylphenol was decreased by using IgG fraction of polyclonal antibodies in combination with lower amount of immobilised 9-(p-hydroxyphenyl)nonanoic acid. Sensitivity of the assay was improved by using a chip with a new derivative on a surface-N-aminobutyl [2-(4-hydroxyphenyl)ethylamine] (limit of detection--5 ng ml(-1)). Applicability of the developed assays to ecological monitoring was checked in experiments using shellfish samples. 4-n-Nonylphenol from spiked samples was extracted into hexane followed by clean-up on NH2 SPE columns. Calibration curves generated for cockles, mussels and oyster samples were identical (limit of detection about 10 ng g(-1)) whereas for scallop samples a slight decrease (about 5-10%) of absolute response was observed. In the assay using the monoclonal antibody specific to 4-n-nonylphenol 31 shellfish samples were found to be negative. Results obtained with polyclonal antibodies indicated that two scallop samples contained a quantity of 4-nonylphenols. The developed biosensor assay could be applied for shellfish analysis as a preliminary screening method. 相似文献
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Host selection,location, and association behaviors of anemonefishes in field settlement experiments 总被引:4,自引:0,他引:4
Nine anemonefish species were reared in the laboratory, and individuals were released in the field (Lizard Island, Australia and Madang, Papua New Guinea) at different distances and orientations away from natural (anemone species the fishes are found with in nature) and unnatural species of host anemones. Experiments were conducted to examine factors that could affect the settlement behaviors of the fishes: current velocity, distance and orientation of the fishes to the anemones, chemical vs visual cues, and presence of conspecific or heterospecific fishes. The fishes were usually attracted toward natural host species of anemones but not towards unnatural host anemone species nor to pieces of dead coral. Host selection during settlement provided the best explanation for the host specificity patterns displayed by anemonefishes in nature. The fishes used chemical cues released from the anemones to identify and locate the appropriate host species and could effectively locate the anemones from a maximum distance of 8 m downstream. Fishes released upstream or to the side of anemones (where anemone chemicals were assumed to be reduced or absent) were much less successful in locating anemones. The ability of the fishes to locate natural host anemones was strongly reduced when there was no water current. The presence of resident anemonefishes on host species of anemones did not influence the attraction behavior of anemonefishes released downstream from the anemones. Once the released fishes got close to or entered the anemones, the resident fishes would generally bite and chase them until the recruits left the anemones. Most fishes were not stung upon initial contact with the anemones. 相似文献
50.
J. H. Martin P. D. Elliott V. C. Anderlini D. Girvin S. A. Jacobs R. W. Risebrough R. L. Delong W. G. Gilmartin 《Marine Biology》1976,35(1):91-104
High premature birth rates have been observed in the rookeries of the California sea lion Zalophus californianus since 1968. The reasons for the premature pupping are complex and, hence, not well understood, although leptospirosis infection and elevated PCB and DDT residues have been implicated. We were interested in determining what role trace and major elements played in these events. Livers and kidneys from 10 normal parturient and 10 premature parturient mothers and their pups were analyzed for Hg, Se, Br, Cd, Ag, Cu, Fe, Zn, Mn, K, Na, Ca, and Mg in order to detect differences that might exist between the two groups. A further objective was to establish how these elements varied in relation to each other in the normal and abnormal sea lions. Our results revealed that Hg, Se, Cd, and Br levels were significantly higher in the livers of the normal mothers and that these elements were all in balance (highly correlated) with each other. This was especially true for Hg, Se, and Br. In mothers with high concentrations of these elements (e.g. Hg greater than 800 g/g dry weight), atomic ratios of approximately 1Hg:1Se:1Br were observed. Atomic Se:Hg ratios were also near unity in the abnormal mothers; however, Br concentrations were always severely depressed in these individuals. Normal full-term pups had higher hepatic levels of Hg and Se, and near-perfect 1:1 Se:Hg atomic ratios were almost always observed. In contrast, the livers of the premature pups appeared to be deficient in Hg, and, consequently, elevated Se:Hg ratios were always found. In almost all cases, the premature pups had increased concentrations of Na, Ca, and Br. Levels of these elements were correlated with their Se:Hg ratios. Amounts of Mn and Cu were reduced in the premature pups and negatively correlated with Se:Hg ratios. The results suggest that balance between elements is of more importance than absolute concentration when the possible effects of toxic elements are considered. It also appears that bromine may be important in the detoxification process involving Se and Hg and perhaps Cd as well; i.e., every mother that had Br in balance with Hg, Cd, and Se had a normal pup, while every mother that lacked sufficient Br had a premature pup. The question of whether Hg detoxifies Se is also raised. All the normal pups had Se:Hg atomic ratios of less than 2.2, while all the premature pups had reduced Hg amounts and Se:Hg ratios above 3.4. 相似文献