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91.
We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The
system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts
using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the
three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells
with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in
complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified
simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with
both natural phytoplankton and microalgal culture samples. 相似文献
92.
Christian Sonne PhD DVM VMD MVSc Rossana Bossi Rune Dietz Pall S. Leifsson Frank F. Rigét Erik W. Born 《毒物与环境化学》2013,95(2):275-283
In this study, residual concentrations of chlorpyrifos (CPF) were determined in feed (40) and fodder (25) samples collected from various locations of Tarai region of Uttarakhand. For extracting residues, liquid–liquid partition followed by alumina column clean up was used and the detection and quantification of residues was undertaken with the help of high-performance liquid chromatography using C18 column and diode array detector at 220?nm. Of the total 40 feed samples analyzed, 7 (17.5%) samples were found positive for CPF with the mean residual concentration of 0.058?µg?g?1; while out of 25 fodder samples, CPF residues were detected in a single (4%) sample with residual concentration of 0.39?µg?g?1. However, none of the feed or fodder samples contained CPF residues above the prescribed limit. 相似文献
93.
Uptake of some selected aquatic pollutants in semipermeable membrane devices (SPMDs) and the polar organic chemical integrative sampler (POCIS) 总被引:2,自引:0,他引:2
Harman C Bøyum O Tollefsen KE Thomas K Grung M 《Journal of environmental monitoring : JEM》2008,10(2):239-247
The uptake characteristics of semipermeable membrane devices (SPMDs) and polar organic chemical integrative samplers (POCISs) were examined for mono, di and tributyltin, triphenyltin, pyrene, benzo[a]pyrene, 4-tert-butylphenol, 4-n-nonylphenol, PCBs 77 and 153, PBDE 47, lindane, triclosan and DDT. Exposure in a flow through system continued for 28 days with samplers removed every 7 days in order to study the relevant uptake kinetics. Uptake remained linear for POCISs with sampling rates (Rs) of up to 0.2 L d(-1). For SPMDs uptake varied from linear to approaching equilibrium with Rs values of up to 14 L d(-1). 7 out of 9 results for SPMDs could be explained by an empirical model (nonylphenol and lindane were exceptions). None of the four organo-tin compounds studied were detected in POCISs and only tributyltin was accumulated significantly by SPMDs. The establishment of these sampling rates allows the calculation of time weighted water concentrations for several important contaminants. Using presented methods, sampling rates and exposure conditions, theoretical detection limits for selected compounds by SPMDs were between 11-68 pg L(-1), which is well below the environment quality standard proposed for those compounds that are included in the European Water Framework Directive. 相似文献
94.
Nadiah P. Kristensen Wei Wei Seah Kwek Yan Chong Yi Shuen Yeoh Tak Fung Laura M. Berman Hui Zhen Tan Ryan A. Chisholm 《Conservation biology》2020,34(5):1229-1240
Extinction is a key issue in the assessment of global biodiversity. However, many extinction rate measures do not account for species that went extinct before they could be discovered. The highly developed island city–state of Singapore has one of the best-documented tropical floras in the world. This allowed us to estimate the total rate of floristic extinctions in Singapore since 1822 after accounting for sampling effort and crypto extinctions by collating herbaria records. Our database comprised 34,224 specimens from 2076 native species, of which 464 species (22%) were considered nationally extinct. We assumed that undiscovered species had the same annual per-species extinction rates as discovered species and that no undiscovered species remained extant. With classical and Bayesian algorithms, we estimated that 304 (95% confidence interval, 213–414) and 412 (95% credible interval, 313–534) additional species went extinct before they could be discovered, respectively; corresponding total extinction rate estimates were 32% and 35% (range 30–38%). We detected violations of our 2 assumptions that could cause our extinction estimates, particularly the absolute numbers, to be biased downward. Thus, our estimates should be treated as lower bounds. Our results illustrate the possible magnitudes of plant extirpations that can be expected in the tropics as development continues. 相似文献
95.
Efficacy of extracting indices from large‐scale acoustic recordings to monitor biodiversity 下载免费PDF全文
Rachel T. Buxton Mary Clapp Erik Meyer Erik Stabenau Lisa M Angeloni Kevin Crooks George Wittemyer 《Conservation biology》2018,32(5):1174-1184
Passive acoustic monitoring could be a powerful way to assess biodiversity across large spatial and temporal scales. However, extracting meaningful information from recordings can be prohibitively time consuming. Acoustic indices (i.e., a mathematical summary of acoustic energy) offer a relatively rapid method for processing acoustic data and are increasingly used to characterize biological communities. We examined the relationship between acoustic indices and the diversity and abundance of biological sounds in recordings. We reviewed the acoustic‐index literature and found that over 60 indices have been applied to a range of objectives with varying success. We used 36 of the most indicative indices to develop a predictive model of the diversity of animal sounds in recordings. Acoustic data were collected at 43 sites in temperate terrestrial and tropical marine habitats across the continental United States. For terrestrial recordings, random‐forest models with a suite of acoustic indices as covariates predicted Shannon diversity, richness, and total number of biological sounds with high accuracy (R2 ≥ 0.94, mean squared error [MSE] ≤170.2). Among the indices assessed, roughness, acoustic activity, and acoustic richness contributed most to the predictive ability of models. Performance of index models was negatively affected by insect, weather, and anthropogenic sounds. For marine recordings, random‐forest models poorly predicted Shannon diversity, richness, and total number of biological sounds (R2 ≤ 0.40, MSE ≥ 195). Our results suggest that using a combination of relevant acoustic indices in a flexible model can accurately predict the diversity of biological sounds in temperate terrestrial acoustic recordings. Thus, acoustic approaches could be an important contribution to biodiversity monitoring in some habitats. 相似文献
96.
Jan Willem Erisman Arjan Hensen Wim de Vries Hans Kros Tamme van de Wal Wim de Winter Jan Erik Wien Mark v. Elswijk Matthijs Maat Kaj Sanders 《Ambio-人类环境杂志》2002,31(2):190-196
利用计算机模拟的方法,我们为第二届国际氮会议研制开发了一个游戏形式的氮决策支持系统.研制这一系统的目的是:①使科学工作者和决策者们都能认识到荷兰氮污染问题的复杂性,因为荷兰是一个农业、工业和运输业活动密集的区域;②探索以最低的社会经济代价解决氮污染的最佳方案.NitroGenius由有关时空范围内的氮流动模型组成,其中包括氨和氮氧化合物的释放及其对地表水和地下水的污染.NitroGenius中还包括了一个经济模型,它描述了各个重要经济部门之间的关系,以及不同氮排放控制措施对国民生产总值(GDP)、失业率、能源消耗和环境的影响.在第二次国际氮会议期间,大约有50个研究组对NitroGenius进行了测试,结果表明,如果认真进行计划并选择适当的治理措施,就能够以适当的代价解决荷兰的氮污染问题. 相似文献
97.
The rain forests of Tanzania occupy small areas, mostly confined to isolated mountains. Except for some isolated volcanic mountains (Mt. Kilimanjaro, Mt. Meru, the Ngorongoro Crater), most of the rain forests are confined to the so-called Eastern Arc mountains with old crystalline bedrocks. The Eastern Arc rain forests are characterized by high biodiversity, and have large numbers of endemic and rare taxa of plants and animals. The submontane and montane rain forests are extremely important water catchments, providing water for major cities and densely populated rural areas. Most of the rain forest catchments are forest reserves, but still the human impact around and inside the reserves is considerable. Intensive cultivation due to heavy population pressure affects the reserves from all sides, leaving the forests isolated, fragmented ‘islands’ surrounded by the cultural landscape. Former timber production and logging programs initiated by the State and some foreign development organizations have depleted large areas of rain forests, e.g. in the Usambara Mountains. Extensive, destructive, legal and illegal activities such as, e.g. logging, pit-sawing and grazing have taken place inside most of the forest reserves. Some research programs on rain forest catchments are now being carried out in Tanzania, forming a basis for future management and monitoring of the forest reserves. The forest authorities are currently much concerned about the problems, giving catchment forest management highest priority in the Tropical Forestry Action Plan. 相似文献
98.
Theoretical study of invasion dynamics has suggested that spatial heterogeneity should strongly influence the rate and extent of spreading organisms. However, empirical support for this prediction is scant, and the importance of understanding heterogeneity for real-world systems has remained ambiguous. This study quantified the influence of host and environmental heterogeneity on the dynamics of a 19-year disease invasion by the exotic and fatal pathogen, Phytophthora lateralis, within a stream population of its host tree, Port Orford cedar (Chamaecyparis lawsoniana). Using dendrochronology, we reconstructed the invasion history along a 1350-m length of infected stream, which serves as the only route of pathogen dispersal. Contrary to theoretical predictions, the temporal progression of the disease invasion was not related to a host's downstream spatial position, but instead was determined by two sources of heterogeneity: host size and proximity to the stream channel. These sources of heterogeneity influenced both the epidemic and endemic dynamics of this pathogen invasion. This analysis provides empirical support for the influence of heterogeneity on the invasion dynamics of a commercially important forest pathogen and highlights the need to incorporate such natural variability into both invasion theory and methods aimed at controlling future spread. 相似文献
99.
A submodel for anaerobic mud-water exchange of phosphate is obtained from experiments in the laboratory. Phosphorus in the sediment can be divided into exchangeable and non-exchangeable phosphorus. The exchangeable phosphorus is decomposed in accordance with a first-order reaction. The phosphorus moves, after the decomposition process, from the interstitial water to the water phase, in accordance with a diffusion expression. The yearly increase of the sediment was determined by means of the lead concentration as a function of the depth. 相似文献
100.
A model for the accumulation of CO2 in the atmosphere has been set up, taking into consideration: (1) the global cycle of nitrogen and phosphorus: (2) the CO2-diffusion in the oceans with means of a multilayer ?ea model; (3) the ability of the oceans to take up CO2; (4) the influence of the CO2-concentrations and of the temperature on this ability; (5) different growth rates for the consumption of fossil fuel including logistic growth; (6) the natural climatic variation.It is shown to be essential to include all these factors. The inclusion of factors (4) and (6), which have been omitted in many previous publications, is very essential to the model. 相似文献