Assessment of Bacterial Degradation of Aromatic Hydrocarbons in the Environment by Analysis of Stable Carbon Isotope Fractionation

¹³C/¹²C stable carbon isotope fractionation was used to assess biodegradation in contaminated aquifers with toluene as a model compound. Different strains of anaerobic bacteria (Thauera aromatica, Geobacter metallireducens, and the sulfate-reducing strain TRM1) showed consistent ¹³C/¹²C carbon isotope fractionation with fractionation factors between C = 1.0017 and 1.0018. In contrast, three cultures of aerobic organisms, using different mono- and dioxygenase enzyme systems to initiate toluene degradation, showed variable isotope fractionation factors of C = 1.0027 (Pseudomonasputida strain mt-2), C = 1.0011 (Ralstonia picketii), andC = 1.0004 (Pseudomonas putida strain F1). The great variability of isotope fractionation between different aerobic bacterial strains suggests that interpretation of isotope data in oxic habitats can only be qualitative. A soil column was run as a model system for contaminated aquifers with toluene as the carbon source and sulfate as the electron acceptor and samples were taken at different ports along the column. Microbial toluene degradation was calculated based on the ¹³C/¹²C isotope fractionation factors of the batch culture experiments together with the observed ¹³C/¹²C isotope shifts of the residual toluene fractions. The calculated percentage of biodegradation, B, correlated well with the decreasing toluene concentrations at the sampling ports and indicated the increasing extent of biodegradation along the column. The theoretical toluene concentrations as calculated based on the isotope values matched the measured concentrations at the different sampling ports indicating that the Rayleigh equation can be used to calculate biodegradation in quasi closed systems based on measured isotope shifts. A similar attempt was performed to assess toluene degradation in a contaminated, anoxic aquifer. A transect of groundwater wells was monitored along the main direction of the groundwater flow and revealed decreasing concentrations accompanied with an increase in the ¹³C/¹²C stable carbon isotope ratio of the residual toluene. Calculation of the extent of biodegradation based on the isotope values and laboratory derived isotope fractionation factors showed that the residual toluene was degraded to more than 99% by microbial activity. Calculation of the theoretical residual toluene concentrations based on the measured isotope values described the strongly decreasing concentrations along the plume. Other aromatic hydrocarbons like benzene and naphthalene which were analysed in the same course also showed decreasing concentrations along the groundwater flow path accompanied by increasing ¹³C values indicating biodegradation.     

Sinks for nitrogen inputs in terrestrial ecosystems: a meta-analysis of 15N tracer field studies

Effects of anthropogenic nitrogen (N) deposition and the ability of terrestrial ecosystems to store carbon (C) depend in part on the amount of N retained in the system and its partitioning among plant and soil pools. We conducted a meta-analysis of studies at 48 sites across four continents that used enriched 15N isotope tracers in order to synthesize information about total ecosystem N retention (i.e., total ecosystem 15N recovery in plant and soil pools) across natural systems and N partitioning among ecosystem pools. The greatest recoveries of ecosystem 15N tracer occurred in shrublands (mean, 89.5%) and wetlands (84.8%) followed by forests (74.9%) and grasslands (51.8%). In the short term (< 1 week after 15N tracer application), total ecosystem 15N recovery was negatively correlated with fine-root and soil 15N natural abundance, and organic soil C and N concentration but was positively correlated with mean annual temperature and mineral soil C:N. In the longer term (3-18 months after 15N tracer application), total ecosystem 15N retention was negatively correlated with foliar natural-abundance 15N but was positively correlated with mineral soil C and N concentration and C:N, showing that plant and soil natural-abundance 15N and soil C:N are good indicators of total ecosystem N retention. Foliar N concentration was not significantly related to ecosystem 15N tracer recovery, suggesting that plant N status is not a good predictor of total ecosystem N retention. Because the largest ecosystem sinks for 15N tracer were below ground in forests, shrublands, and grasslands, we conclude that growth enhancement and potential for increased C storage in aboveground biomass from atmospheric N deposition is likely to be modest in these ecosystems. Total ecosystem 15N recovery decreased with N fertilization, with an apparent threshold fertilization rate of 46 kg N x ha(-1) x yr(-1) above which most ecosystems showed net losses of applied 15N tracer in response to N fertilizer addition.     

Mass occurrence of Salpa fusiformis in the spring of 1984 off Ireland: implications for sedimentation processes

Zooplankton species composition and biomass were investigated during the spring of 1984 in three areas west of Ireland. In general, biomass of the gelatinous zooplankters [Salpa fusiformis (Cuvier) forma gregata and solitaria, Cymbulia sp., Euclio sp.; max. 360 mg Cm^-3] exceeded that of other zooplankton namely copepods (max. 70 mg C m^-3). Feeding by salps in the upper layers of all areas during the observed diatom spring bloom resulted in sedimentation of diatom-rich salp fecal pellets. This process ended the diatom spring bloom prior to nutrient depletion in surface waters and, thus, prior to mass sedimentation of algal cells.Publication No. 17 of the SFB 313 at Kiel University     

Mercury in various tissues of three mustelid species and other trace metals in liver of European otter from Eastern Finland

Mercury concentrations were monitored in European otter (Lutra lutra), European polecat (Mustela putorius) and European pine marten (Martes martes) collected in Eastern Finland during the period 1972–2008. Otters mainly eat fish, which is an important reason to monitor the bioaccumulation of mercury in this predator. In this species, the highest concentrations were found in fur followed by liver and kidney, and the mercury concentrations increased with increasing age and body weight. Males showed in general higher concentrations than females of otters. The food of European polecat consists of small mammals, frogs, birds and insects from both aquatic and terrestrial food chains. The mercury concentrations were lower than in otters without significant differences related to body weight or sex. In European pine martens, the concentrations were rather evenly distributed except for two specimens with high concentrations. Also, concentrations of some other metals (Al, Cd, Cu, Fe, Mn, Ni, Pb and Zn) were analysed from liver samples of otter. Possible adverse effects of mercury on the Finnish populations of these mustelids are discussed.     

Distribution of selected carcinogenic hydrocarbon and heavy metals in an oil-polluted agriculture zone

Owing to the importance of clean and fertile agricultural soil for the continued existence of man, this study investigated the concentrations of total petroleum hydrocarbons (TPHs), polycyclic aromatic hydrocarbons (PAHs) and some heavy metals in soils and selected commonly consumed vegetables and tubers from oil-polluted active agricultural farmland in Gokana of Ogoniland, Rivers State, Nigeria. Samples from Umuchichi, Osisioma Local Government Area in Abia State, Nigeria, a non-oil-polluted area constituted the control. In test and control, up to 3,830?±?19.6 mgkg^?1 dw and 6,950?±?68.3 mgkg^?1 dw (exceeding DPR set limits) and 11.3?±?0.04 mgkg^?1 dw and 186?±?0.02 mgkg^?1 dw for TPH and PAHs, respectively, were recorded in test soil and plant samples, respectively. Among the metals studied (Pb, Cd, Cr, Mn, Fe and Zn), Pb and Cr uptake exceeded WHO set limits for crops in test samples. Combined sources of pollution were evident from our studies. Bitterleaf and Waterleaf could be tried as bioindicators owing to expressed contaminants uptake pattern.     

(3R,4S)-Methyl-3-heptanol,the trail pheromone of the ant Leptogenys diminuta

We wish to thank Prof. V. Schurig (Tübingen) for lending us the columns for preliminary studies, and Prof. K. Mori (Tokyo), N. Frighetto and R. T. S. Frighetto (Erlangen) for optically active samples of 4-methyl-3-heptanol. This work was supported by the Deutsche Forschungsgemeinschaft.     

Phenolic xenoestrogens in surface water, sediments, and sewage sludge from Baden-Württemberg, south-west Germany.

Nine structurally different phenolic chemicals, which have been reported to mimic estrogen effects, were determined in various aquatic environmental compartments. Twenty-three water samples from five streams and rivers showed levels up to 458 ng/l for 4-nonylphenol (4NP), 189 ng/l for 4-t-octylphenol (4tOP), 272 ng/l for bisphenol A (BPA) and 47 ng/l for 2-hydroxybiphenyl (2OHBiP). Elevated levels of these compounds in a stream with a high load of effluents of sewage treatment plants (STPs), compared to a brook free of sewage, identified STPs as major sources. With a similar order, 4NP (10-259 micrograms/kg dry matter), 4tOP (< 0.5-8 micrograms/kg), BPA (< 0.5-15 micrograms/kg), and 2OHBiP (2-69 micrograms/kg) were also detected regularly in riverine sediment (n = 11). Levels in sewage sludge were one order of magnitude higher than in sediments. 4-Hydroxybiphenyl and 4-chloro-3-methylphenol were found predominantly in sludge and sediment in the lower ppb range.     

No evidence for the work-conflict hypothesis in the eusocial naked mole-rat (Heterocephalus glaber )

The work-conflict hypothesis (Reeve 1992) postulates that in eusocial naked mole-rat (Heterocephalus glaber) colonies there is potential conflict between the breeding female and her non-breeding colony mates over how much aid they provide in support of her reproduction. Non-breeders may withhold aid either because they are hopeful reproductives and/or because they have less to gain in terms of inclusive fitness. Breeding females should, therefore, preferentially direct aggression in the form of shoving (prolonged pushes usually involving nose-to-nose contact) towards large (hopeful reproductives) and less related non-breeders to incite them to provide aid. This study tested the following predictions of the work-conflict hypotheses. (1) There is kin bias in the shove rate of breeding females and work rates of non-breeders; (2) Shoving activates work in non-breeders. We found no evidence to suggest that the breeding female shove rate and the amount of colony maintenance activities performed by non-breeders is influenced by relatedness. Body size was a better predictor of work rate and shove rate; large animals were shoved more and worked less. Furthermore, shoving did not affect the work rate of non-breeders and rarely incited activity in inactive mole-rats. Individual mole-rats were also more likely to be shoved when already active than when resting in the nest. These results suggest that shoving by the breeding female may have functions other than the incitement of activity, such as the reproductive suppression of non-breeders. Received: 17 January 1996 / Accepted after revision: 21 September 1996     

Temporal patterns of road network development in the Brazilian Amazon

The Brazilian Amazon is a globally important ecosystem that is undergoing rapid development and land-use change. Roads are a key spatial determinant of land-use conversion and strongly influence the rates and patterns of habitat loss and represent a key component of models that attempt to predict the spatio-temporal patterns of Amazonian land-use change and the consequences of such changes. However, the spatio-temporal patterns of road network development are poorly understood and seldom quantified. Here, we used manually digitised satellite imagery at multiple temporal and spatial scales across the Brazilian Amazon to quantify and model the rate at which road networks are proliferating. We found that the road network grew by almost 17,000 km per year between 2004 and 2007. There was large spatial variation in road network density, with some municipalities having road densities as high as 0.5 km/km², and road network growth rates were highest in municipalities with an intermediate road network density. Simulations indicated that road network development within municipalities follows a logistic growth pattern through time, with most of the development occurring within a 39-year time period. This time period is similar to those of other boom and bust development dynamics observed in the Brazilian Amazon. Understanding the temporal patterns of road development will aid the development of better predictive land-use change models for the Amazon, given the key importance of roads as a predictor of deforestation in many existing models.