Identification of two mutations for ataxia telangiectasia among the Druze community

Ataxia telangiectasia (AT) is a rare autosomal recessive disease characterized by progressive cerebellar ataxia, immunodeficiency, susceptibility to lymphoreticular malignancies and cancer predisposition, hypersensitivity to ionic radiation and chromosomal instability. In this study, we report a founder effect of AT with two different mutations: 1339 C > T and 6672 del GG together with 6677 del TACG, found in four Israeli Druze clans originating from three different Druze centers in the Middle East (Lebanon, Syria and Jordan). The 1339 C > T mutation, which results in a stop codon at position 447 of the ATM protein, was observed in two unrelated clans originating from Lebanon and Jordan. The 6672 del GG/6677 del TACG mutation was observed in two unrelated clans originating from Syria and Lebanon. In the present study, simple and fast detection assays were developed for both mutations. The ability to identify AT carriers routinely provides a unique opportunity for prenatal diagnosis, genetic counseling as well as marriage guidance in the Druze community. Copyright © 2004 John Wiley & Sons, Ltd.     

Effects of Rainfall and Ground‐Water Pumping on Streamflow in Mākaha,O’ahu,Hawai’i1

Abstract: Land‐use/land‐cover changes in Mākaha valley have included the development of agriculture, residential dwellings, golf courses, potable water supply facilities, and the introduction of alien species. The impact of these changes on surface water and ground water resources in the valley is of concern. In this study, streamflow, rainfall, and ground‐water pumping data for the upper part of the Mākaha valley watershed were evaluated to identify corresponding trends and relationships. The results of this study indicate that streamflow declined during the ground‐water pumping period. Mean and median annual streamflow have declined by 42% (135 mm) and 56% (175 mm), respectively, and the mean number of dry stream days per year has increased from 8 to 125. Rainfall across the study area appears to have also declined though it is not clear whether the reduction in rainfall is responsible for all or part of the observed streamflow decline. Mean annual rainfall at one location in the study area declined by 14% (179 mm) and increased by 2% (48 mm) at a second location. Further study is needed to assess the effect of ground‐water pumping and to characterize the hydrologic cycle with respect to rainfall, infiltration, ground‐water recharge and flow in the study area, and stream base flow and storm flow.     

Evaluation of phytotoxicity of three organic amendments to collard greens using the seed germination bioassay

Environmental Science and Pollution Research - Small-scale vegetable and fruit crop producers in the USA use locally available commercial organic fertilizers and soil amendments recycled from...     

Poly(Acrylic Acid) Grafted Sodium Alginate Di-Block Hydrogels as Efficient Biosorbents; Structure-Property Relevance

Naturally-based poly(acrylic acid) grafted sodium alginate di-block hydrogels were investigated as high efficiency biosorbents for copper(II) ion. The grafted di-block hydrogel was characterized using FTIR, TGA and SEM techniques. Blank and immobilized algal biosorbent beads formed via 2.0% (w/w) calcium ions were also investigated. Batch adsorption experiments revealed optimal pH dependence of copper(II) ion biosorption at pH 5.5 with high efficient copper(II) ion uptake of 98.5 mg/g. The dynamics studies showed that the high efficiency copper(II) ion biosorption followed pseudo-second order kinetics with significant contribution of intraparticle diffusion mechanism. The equilibrium data fitted to Langmuir, Freundlich, and Dubinin–Radushkevich (D–R) adsorption isotherm models. Thermodynamics parameters for copper(II) biosorption on blank and immobilized algal beads depicted the spontaneous nature of the biosorption process. Such high efficiency, feasibility, simplicity, and low cost properties adapt the di-block biosorbent to be the next generation promising biosorbents for water decontamination and to help in the recovery of the missing ecologic harmony.     

Effects of Spatial and Temporal Data Aggregation on the Performance of the Multi‐Radar Multi‐Sensor System

The objectives of this study were to (1) evaluate the performance of the Multi‐Radar Multi‐Sensor (MRMS) system in capturing precipitation compared to gauge data, and (2) assess the effects of spatial (1–50 km) and temporal (15–120 min) data aggregation scales on the performance of the MRMS system. Point‐to‐grid comparisons were conducted between 215 rain gauges and the MRMS system. The MRMS system at 1 km spatial and 15 min temporal resolutions captured precipitation reasonably well with average R², root mean square error (RMSE), and percent bias (PBIAS) values of 0.65, 0.5 mm, and 11.9 mm; whereas Threat Score, probability of detection, and false alarm ratio were 0.57, 0.92, and 0.40, respectively. Decreasing temporal resolution from 15 min to two hours resulted in an increase in R² and a decrease in RMSE, whereas PBIAS was not affected. Reducing spatial resolution from 1 to 50 km resulted in increases in R² and PBIAS, whereas RMSE was decreased. Increasing spatial aggregation scale from 1 to 50 km resulted in an R² increase of only 0.08. Similarly, improvement in R² was only modest (0.17) compared to an eightfold reduction in temporal resolution (from 15 min to two hours). While aggregating data at coarser temporal resolutions resolved some of the under/overestimation issues of the MRMS system, it was apparent even at coarser spatial and temporal resolutions the MRMS system inherently overestimated smaller precipitation events while underestimated bigger events.     

Trans-ferulic acid ameliorates cisplatin-induced testicular damage via suppression of TLR4, P38-MAPK,and ERK1/2 signaling pathways

Environmental Science and Pollution Research - Testicular damage has been described as a common side effect of cisplatin (CDDP), which limits its clinical uses. Since oxidative injury and...     

Health care system hazard vulnerability analysis: an assessment of all public hospitals in Abu Dhabi

Hazard vulnerability analysis (HVA) is used to risk‐stratify potential threats, measure the probability of those threats, and guide disaster preparedness. The primary objective of this project was to analyse the level of disaster preparedness in public hospitals in the Emirate of Abu Dhabi, utilising the HVA tool in collaboration with the Disaster Medicine Section at Harvard Medical School. The secondary objective was to review each facility's disaster plan and make recommendations based on the HVA findings. Based on the review, this article makes eight observations, including on the need for more accurate data; better hazard assessment capabilities; enhanced decontamination capacities; and the development of hospital‐specific emergency management programmes, a hospital incident command system, and a centralised, dedicated regional disaster coordination centre. With this project, HVAs were conducted successfully for the first time in health care facilities in Abu Dhabi. This study thus serves as another successful example of multidisciplinary emergency preparedness processes.     

Ozone uptake by citrus trees exposed to a range of ozone concentrations

The Citrus genus includes a large number of species and varieties widely cultivated in the Central Valley of California and in many other countries having similar Mediterranean climates. In the summer, orchards in California experience high levels of tropospheric ozone, formed by reactions of volatile organic compounds (VOC) with oxides of nitrogen (NO_x). Citrus trees may improve air quality in the orchard environment by taking up ozone through stomatal and non-stomatal mechanisms, but they may ultimately be detrimental to regional air quality by emitting biogenic VOC (BVOC) that oxidize to form ozone and secondary organic aerosol downwind of the site of emission. BVOC also play a key role in removing ozone through gas-phase chemical reactions in the intercellular spaces of the leaves and in ambient air outside the plants. Ozone is known to oxidize leaf tissues after entering stomata, resulting in decreased carbon assimilation and crop yield. To characterize ozone deposition and BVOC emissions for lemon (Citrus limon), mandarin (Citrus reticulata), and orange (Citrus sinensis), we designed branch enclosures that allowed direct measurement of fluxes under different physiological conditions in a controlled greenhouse environment. Average ozone uptake was up to 11 nmol s^?1 m^?2 of leaf. At low concentrations of ozone (40 ppb), measured ozone deposition was higher than expected ozone deposition modeled on the basis of stomatal aperture and ozone concentration. Our results were in better agreement with modeled values when we included non-stomatal ozone loss by reaction with gas-phase BVOC emitted from the citrus plants. At high ozone concentrations (160 ppb), the measured ozone deposition was lower than modeled, and we speculate that this indicates ozone accumulation in the leaf mesophyll.     

Volatile organic compounds from Italian vegetation and their interaction with ozone

Volatile Organic Compounds (VOCs) emitted from vegetation (particularly isoprenoids) represent an important source of atmospheric hydrocarbons almost double the anthropogenic source. When biogenic VOC mix with NO_x in the presence of UV radiation, ozone (O₃) is formed. In Italy, optimal conditions for O₃ formation in terms of VOC/NO_x ratios and abundance of UV radiation occur for long periods of the year. Moreover, Italian vegetation includes several species that are strong and evergreen isoprenoid emitters, and high temperatures for part of the year further stimulate these temperature-dependent emissions. We review emission of isoprenoids from Italian vegetation, current knowledge on the impact of rising O₃ levels on isoprenoid emission, and evidence showing that isoprenoids can increase both the O₃ flux to the plant and protection against oxidative stress because of their antioxidant functions. This trait not only influences plant tolerance to O₃ but also may substantially alter the flux of O₃ between atmosphere and biosphere.     

Carbon dioxide emission in relation with irrigation and organic amendments from a sweet corn field

Soil moisture and organic matter level affects soil respiration and microbial activities, which in turn impact greenhouse gas (GHG) emissions. This study was conducted to evaluate the effect of irrigation levels (75% [deficit], 100% [full], and 125% [excess] of reference crop evapotranspiration requirements), and organic amendments (OA) type (chicken manure [CM] and bone meal [BM]) and OA application rates (0,168, 336 and 672 kg total N ha^?1) on (i) soil physical properties (bulk density, organic matter content and soil moisture content) and (ii) soil carbon dioxide (CO₂) emissions from a highly weathered tropical Hawai'ian soil. Carbon dioxide readings were consistently taken once or twice a week for the duration of the cropping season. A drip irrigation system was used to apply the appropriate amount of irrigation water to the treatment plots. Treatments were randomly selected and corresponding organic amendments were manually incorporated into the soil. Plots were cultivated with sweet corn (Zea mays ‘SS-16’). Soil moisture content within and below the rootzone was monitored using a TDR 300 soil moisture sensor (Spectrum Technologies, Inc., Plainfield, IL, USA) connected with 12 cm long prongs. Soil bulk density and organic matter content were determined at the end of the cropping season. Analysis of variance results revealed that OA type, rate, and their interaction had significant effect on soil CO₂ flux (P < 0.05). Among the OA rates, all CM mostly resulted in significantly higher soil CO₂ fluxes compared to BM and control treatment (p < 0.05). The two highest rates of BM treatment were not significantly different from the control with regard to soil CO₂ flux. In addition, organic amendments affected soil moisture dynamics during the crop growing season and organic matter content measured after the crop harvest. While additional studies are needed to further investigate the effect of irrigation levels on soil CO₂ flux, it is recommended that in order to minimize soil CO₂ emissions, BM soil amendments could be a potential option to reduce soil CO₂ fluxes from agricultural fields similar to the one used in this study.