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981.
982.
R. B. H. Schutgens G. Schrakamp R. J. A. Wanders H. S. A. Heymans H. W. Moser A. E. Moser J. M. Tagers H. V. D. Bosch P. Aubourm 《黑龙江环境通报》1985,5(5):337-344
Prenatal diagnosis of the cerebro-hepato-renal (Zellweger) syndrome has been performed in 10 pregnancies at risk by measuring both the activity of acyl CoA: dihydroxyacetonephosphate acyltransferase (DHAP-AT) and the de novo plasmalogen biosynthesis, either in cultured amniotic fluid cells or in fibroblasts cultured from a chorionic villus biopsy. In 7 of the pregnancies both tests indicated no abnormality. All 7 continued to term and normal infants were delivered. However, in amniotic fluid cells from 2 fetuses affected by Zellweger syndrome unequivocal differences from control values were found. The activity of DHAP-AT was clearly deficient and the de novo plasmalogen biosynthesis was impaired. In one pregnancy at risk prenatal diagnosis was performed during the first trimester by measuring both the DHAP-AT activity and the de novo plasmalogen biosynthesis in fibroblasts cultured from a chorionic villi biopsy. From the deficient DHAP-AT activity and the impaired de novo plasmalogen biosynthesis it was concluded that the fetus was affected. This was confirmed biochemically after induced abortion. It can be concluded that measurement of the DHAP-AT activity and the de novo plasmalogen biosynthesis provides convenient methods for the early prenatal detection of Zellweger syndrome. 相似文献
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The aim of this study was to determine whether there is increased leakage of neuron-specific enolase (NSE) and S-100 protein into amniotic fluid in pregnancies with neural tube defects, since both these proteins are produced by neural tissue, and to compare the value of these substances for detecting such defects with that of the more conventional techniques of alpha-fetoprotein (AFP) and acetylcholinesterase (AChE) gel electrophoresis. Amniotic samples from 25 mid-pregnancies (15–17 weeks' gestation) with neural tube defects (14 with open spina bifida and 11 with anencephaly) and from seven mid-pregnancies with abdominal wall defects were compared with a control material consisting of 80 amniotic fluid samples from 80 consecutive mid-pregnancy amniocenteses, with normal karyotypes and AFP concentrations. All of the above cases of abnormalities were primarily detected through increased AFP levels in the amniotic fluid. Amniotic fluid samples from 13 pregnancies with fetuses with autosomal chromosomal abnormalities and seven amniotic fluid samples contaminated with blood were also included in the investigation. It is concluded from the results that the conventional AFP assay combined with AChE gel electrophoresis is the best method for screening amniotic fluid for neural tube defects and defects of the abdominal wall. Neither NSE nor S-100 assay alone proved to be superior for the detection of these cases in mid-trimester amniotic fluid. The S-100 assay, however, could give additional information in cases where AChE gel electrophoresis is not decisive; for example, in samples contaminated with blood. 相似文献
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MATTHEW SOMMERVILLE E.J. MILNER‐GULLAND MICHAEL RAHAJAHARISON JULIA P.G. JONES 《Conservation biology》2010,24(6):1488-1498
Abstract: Despite the growing interest in conservation approaches that include payments for environmental services (PES), few evaluations of the influence of such interventions on behaviors of individuals have been conducted. We used self‐reported changes in six legal and illegal forest‐use behaviors to investigate the way in which a PES for biodiversity conservation intervention in Menabe, Madagascar, influenced behavior. Individuals (n =864) from eight intervention communities and five control communities answered questions on their forest‐use behaviors before and after the intervention began, as well as on their reasons for changing and their attitudes to various institutions. The payments had little impact on individuals’ reported decisions to change behaviors, but it had a strong impact on individuals’ attitudes. Payments appeared to legitimize monitoring of behaviors by the implementing nongovernmental organization (NGO), but did not act as a behavioral driver in their own right. Although there were no clear differences between changes in behaviors in the intervention and control communities, the intervention did influence motivations for change. Fear of local forest associations and the implementing NGO were strong motivators for changing behavior in communities with the PES intervention, whereas fear of the national government was the main reason given for change in control communities. Behavioral changes were most stable where fear of local organizations motivated the change. Our results highlight the interactions between different incentives people face when making behavioral decisions and the importance of considering the full range of incentives when designing community‐based PES interventions. 相似文献
990.
Micro-irrigation systems (MIS) have been at the forefront of policy-making and social research in exploring determinants that could potentially impact the adoption of MIS technologies in the field to fulfil the basic aim of enhanced agricultural productivity and enriched nutritional quality of the produce with optimal adoption of natural resources. Therefore, this study was undertaken to determine why MIS technologies have not been adopted to the extent anticipated, so that suitable policy schemes, promotional schemes and socio-technical frameworks could be formulated for their enhanced adoption to enhance the socio-economic status of the farming community in the Dahod district of Gujarat State, India. A study of 350 non-MIS (NMIS) and 350 MIS farmers was conducted to identify factors affecting the MIS adoption process. The logit model was fitted using XLSTAT software (XLSTAT 2014.1.04) to the explanatory variables (determinants) of the MIS adoption process. Type III analysis and ANOVA were conducted to test the relative significance of the explanatory variables adopted. It was found that total income had the highest weight (or beta coefficient, i.e. 0.625) followed by total land area (0.546), motor horsepower (0.499), dependency ratio (0.397), and education (0.295) and age of household head (0.207). Furthermore, to assess the efficacy of the logit model, the ROC curve was also developed and the AUC was found to be 0.881, and therefore the model was considered to discriminate well in identifying the factors affecting the MIS adoption process. The study found that higher total income and education level increase the likelihood of MIS adoption and agricultural water management, and therefore special training programmes on installation, as well as repair and maintenance, of MIS systems and agricultural water management can be planned at the institutional/organisation level. The total cultivable area is also one of the important determinants in MIS adoption, and therefore the adoption of MIS schemes should not be restricted to large farmers only, but rather should be extended to both small and marginal farmers. 相似文献