CASA DEL AGUA: WATER CONSERVATION DEMONSTRATION HOUSE 1986 THROUGH 19981

ABSTRACT: Casa del Agua (Casa) in suburban Tucson, Arizona, was designed as a residential water conservation facility for applied research, demonstration of operational results, and transfer of technology to the general public. Starting in 1983, an existing residence was located, modified and retrofitted to acquire operational data on residential water use. Modifications included retrofitting existing landscapes and enlarging the rooftop to collect and harvest rainwater; separating blackwater and graywater lines; installing meters, low‐water‐use appliances and fixtures, and underground storage tanks for rainwater and graywater; and creating a public information center. Over the 13‐plus years of actual operation, both the interior and exterior water use research results indicate large reductions in water use can be effected using water‐saving devices andlor harvesting and reusing rainwater and graywater. Casa achieved over a 24 percent reduction in total water use and a 47 percent reduction in municipal water used compared to the typical Tucson residence. Overall water used was comprised of harvested rainwater (10 percent), recycled graywater (20 percent), and municipal water (70 percent). Casa's Information Center was visited by approximately 13,000 people from September 1985 through April 1999 and the research has been featured in local, national, and international media.     

Molecular phylogenetic analyses of shallow-water Caribbean octocorals

Octocorals, especially gorgonians, are conspicuous on Caribbean coral reefs, but there is no consensus regarding species relationships. Mitochondrial protein-coding genes [NADH-dehydrogenase subunits 2 (ND2) and 6 (ND6), and mutS homolog (msh1), 1633 bp] from 28 shallow-water species were sequenced to develop the first molecular phylogeny for Caribbean octocorals. The specimens were collected primarily in the Caribbean or off Brazil in 1999-2001. Morphological characters (sclerites and axial ultrastructure) were also examined in order to map them onto the molecular phylogeny. Analyses of both nucleotide and amino acid substitutions using maximum parsimony and likelihood (including maximum-likelihood and Bayesian analysis) generated very similar results, with most nodes having high levels of support. These molecular results were significantly different from the generally accepted classification. Neither Plexauridae nor Gorgoniidae were monophyletic. Plexaurella spp., nominal plexaurids, were basal to the gorgoniids, sharing many morphological characters with them. This corroborates previous findings using secondary metabolites and biosynthetic pathways. The sea fans, Gorgonia spp. and Pacifigorgia spp., as well as the pinnate gorgonians, Muriceopsis flavida and Pseudopterogorgia spp., did not have sea fan or pinnate relatives, suggesting there has been convergent evolution of colony form. Caribbean plexaurids appeared more derived and/or recently evolved according to both morphological and molecular data (e.g. Eunicea spp. and Plexaura spp.). Molecular phylogenetics is a promising approach for reconstructing phylogenetic relationships among octocorals as well as to understand their complex morphology. Electronic Supplementary Material is available if you access this article at http://dx.doi.org/10.1007/s00227-003-1018-7. On that page (frame on the left side), a link takes you directly to the supplementary material.