A morphological approach for relating decapod crustacean cephalothorax shape with distribution in the water column

Cephalothorax profiles for individuals of 18 decapod crustacean species were obtained from pictures of specimens captured using various experimental bottom trawls in the Mediterranean basin, at depths ranging from 200 to 4000 m in 2000 and 2002. Profiles were compared using the shape (outline) of the entire cephalothorax (including and excluding the rostrum). Principal component analysis (PCA) of the profiles, rostrum included, yielded two large species groupings related to rostrum size, species having a long rostrum being clustered on the positive portion of the first axis and species having a short rostrum being clustered on the negative portion of this same axis (the F1 axis explained 72.17% of the total variance). The PCA separated pelagic and endobenthic animals (with short rostrums) from nektobenthic animals (with long rostrums). Only the two deepest-dwelling species (dwelling at depths below 2000 m) were distanced from their respective groups. The pelagic shrimp Acanthephyra pelagica was clearly grouped with the nektobenthic species despite being reported to have a pelagic habitat. The nektobenthic deep-sea species Nematocarcinus exilis has a relatively medium-size rostrum and was placed among the pelagic–endobenthic species. The endobenthic and pelagic groups remained stable even when rostral morphometrics were excluded from the PCA analysis. The hypothesis that predation and diet are the fundamental determining factors for all habitats throughout most of the lifetimes of individuals and hence that species will evolve specialised phenotypes to adapt to local environmental conditions is considered in the discussion.Communicated by O. Kinne, Oldendorf/Luhe     

Spatial and temporal patterns of predation on seeds of the surfgrass Phyllospadix torreyi

Spatial and temporal patterns of predation on seeds of the seagrass Phyllospadix torreyi S. Watson were quantified at four sites near Santa Barbara, California, USA. Over a period of four flowering seasons during 1995 to 1998, monthly patterns of seed fall and intensity of seed predation were similar among sites, but were temporally quite variable. Abundance of dispersed seeds varied greatly both among seasons and years. Within any one year, seeds were present in the environment every month, but they peaked in abundance during the fall months following the annual flowering period. Seeds were more abundant during the earlier years of the study. The intensity of predation steadily increased throughout the study period, from a low of ≤10% seeds consumed during 1995 to ∼50% consumption by 1997, and it was not correlated with abundance of seeds in the environment. Pre-dispersal seed loss also was estimated in two flowering seasons by counting the numbers of seeds consumed prior to release from the plant, and was relatively low (<15%). No differences were detected among the four study sites in patterns of pre-dispersal loss. Field surveys were done at two sites to identify potential seed predators. Three of the most abundant species identified in these surveys were tested in the laboratory to determine size-specific patterns of feeding activity and to assess which early life stages of P. torreyi (fruits within spadices, dehisced fruits, seedlings) were most vulnerable to predation. All three of the early life stages were consumed. The crabs Pugettiaproducta (Randall) and Pachygrapsus crassipes Randall were much more voracious predators than the isopod Idotea resecata Stimpson. Received: 8 June 1999 / Accepted: 2 February 2000     

A basidiomycete isolated from the skeleton of<Emphasis Type="Italic"> Pocillopora damicornis</Emphasis> (Scleractinia) selectively stimulates short-term survival of coral skeletogenic cells

Endolithic fungi bore through the extracellular calcium carbonate skeleton of reef-building scleractinian corals, both healthy and dead, and effect net erosion of coral reefs. Potential fungal interactions with coral tissue were investigated using an in vitro approach suggested by earlier observations of skeletal repair cones at the site of fungal perforation in Porites sp. A fungal strain was isolated from the skeleton of a long-term culture of healthy, tissue-covered, Pocillopora damicornis Linnaeus colonies maintained in a recirculating system in Monaco. As coral soft tissue spontaneously dissociated in vitro, the skeleton became exposed and hyaline hyphae emerged radially from 15% of the total clipped branches. In this study, which was performed between January 2001 and March 2003, 35 skeleton–hypha explants were embedded in agar-based solid medium, yielding 60% hyphal growth. A fungal strain (F19-3-1) of the dominant (80%) morphology was isolated and propagated in agar-based solid medium. The strain was identified by 18S and 26S rDNA gene sequence analysis as a basidiomycete in the genus Cryptococcus. Co-cultures were used to provide experimental exposure of coral soft tissue to the fungus. The fungus extended the survival of coral cells by 2 days, selectively maintaining skeletogenic cell types. This effect may be interpreted as stimulation by the fungus of a short-term coral defense response.Communicated by J.P. Grassle, New Brunswick     

Potassium carbonate-based ternary transition temperature mixture (deep eutectic analogues) for CO2 absorption: Characterizations and DFT analysis

•Addition of hindered amine increased thermal stability and viscosity of TTTM. •Addition of hindered amine improved the CO₂ absorption performance of TTTM. •Good the CO₂ absorption of recycled solvents after two regenerations. •Important role of amine group in CO₂ absorption of TTTM confirmed by DFT analysis. Is it possible to improve CO₂ solubility in potassium carbonate (K₂CO₃)-based transition temperature mixtures (TTMs)? To assess this possibility, a ternary transition-temperature mixture (TTTM) was prepared by using a hindered amine, 2-amino-2-methyl-1,3-propanediol (AMPD). Fourier transform infrared spectroscopy (FT-IR) was employed to detect the functional groups including hydroxyl, amine, carbonate ion, and aliphatic functional groups in the prepared solvents. From thermogravimetric analysis (TGA), it was found that the addition of AMPD to the binary mixture can increase the thermal stability of TTTM. The viscosity findings showed that TTTM has a higher viscosity than TTM while their difference was decreased by increasing temperature. In addition, Eyring’s absolute rate theory was used to compute the activation parameters (ΔG*, ΔH*, and ΔS*). The CO₂ solubility in liquids was measured at a temperature of 303.15 K and pressures up to 1.8 MPa. The results disclosed that the CO₂ solubility of TTTM was improved by the addition of AMPD. At the pressure of about 1.8 MPa, the CO₂ mole fractions of TTM and TTTM were 0.1697 and 0.2022, respectively. To confirm the experimental data, density functional theory (DFT) was employed. From the DFT analysis, it was found that the TTTM+ CO₂ system has higher interaction energy (|ΔE |) than the TTM+ CO₂ system indicating the higher CO₂ affinity of the former system. This study might help scientists to better understand and to improve CO₂ solubility in these types of solvents by choosing a suitable amine as HBD and finding the best combination of HBA and HBD.     

Genomic erosion in a demographically recovered bird species during conservation rescue

The pink pigeon (Nesoenas mayeri) is an endemic species of Mauritius that has made a remarkable recovery after a severe population bottleneck in the 1970s to early 1990s. Prior to this bottleneck, an ex situ population was established from which captive-bred individuals were released into free-living subpopulations to increase population size and genetic variation. This conservation rescue led to rapid population recovery to 400–480 individuals, and the species was twice downlisted on the International Union for the Conservation of Nature (IUCN) Red List. We analyzed the impacts of the bottleneck and genetic rescue on neutral genetic variation during and after population recovery (1993–2008) with restriction site-associated sequencing, microsatellite analyses, and quantitative genetic analysis of studbook data of 1112 birds from zoos in Europe and the United States. We used computer simulations to study the predicted changes in genetic variation and population viability from the past into the future. Genetic variation declined rapidly, despite the population rebound, and the effective population size was approximately an order of magnitude smaller than census size. The species carried a high genetic load of circa 15 lethal equivalents for longevity. Our computer simulations predicted continued inbreeding will likely result in increased expression of deleterious mutations (i.e., a high realized load) and severe inbreeding depression. Without continued conservation actions, it is likely that the pink pigeon will go extinct in the wild within 100 years. Conservation rescue of the pink pigeon has been instrumental in the recovery of the free-living population. However, further genetic rescue with captive-bred birds from zoos is required to recover lost variation, reduce expression of harmful deleterious variation, and prevent extinction. The use of genomics and modeling data can inform IUCN assessments of the viability and extinction risk of species, and it helps in assessments of the conservation dependency of populations.     

Modeling Diapause Termination of Rhipicephalus Appendiculatus using Statistical Tools to Detect Sudden Behavioral Changes and Time Dependencies

This paper presents statistical methodology to analyze longitudinal binary responses for which a sudden change in the response occurs in time. Probability plots, transition matrices, and change-point models and more advanced techniques such as generalized auto-regression models and hidden Markov chains are presented and applied on a study on the activity of Rhipicephalus appendiculatus, the major vector of Theileria parva, a fatal disease in cattle. This study presents individual measurements on female R. appendiculatus, which are terminating their diapause (resting status) and become active. Comprehending activity patterns is very important to better understand the ecology of R. appendiculatus. The model indicates that activity and non-activity act in an absorbing way meaning that once a tick becomes active it shows a tendency to remain active. The change-point model estimates that the sudden change in activity happens on December 10. The reaction of ticks on acceleration and changes in rainfall and temperature indicates that ticks can sense climatic changes. The study revealed the underlying not visually observable states during diapause development of the adult tick of R. appendiculatus. These states could be related to phases during the dynamic event of diapause development and post-diapause activity in R. appendiculatus.     

The temporal changes of the concentration level of typical toxic organics in the river sediments around Beijing

In this study, the current situation of five types of toxic organics and endocrine disrupters in the sediments of rivers around Beijing, i.e., polycyclic aromatic hydrocarbons (PAHs), phthalic acid esters (PAEs), organic chlorinated pesticides (OCPs), estrogens (Es), and bisphenol A (BPA), which included 56 contaminants, was analyzed and compared with that registered by the historical literatures. The ecological risks were also assessed. The total concentration of PAHs, PAEs, OCPs, Es, and BPA ranged from 232.5 ng·g^–1 to 5429.7 ng·g^–1, 2047.2 ng·g^–1 to 18051.5 ng·g^–1, 4.5 ng·g^–1 to 11.7 ng·g^–1, 18.1 ng·g^–1 to 105.2 ng·g^–1, and 36.3 ng·g^–1 to 69.6 ng·g^–1, respectively. Among these five types of organic compounds, the concentration levels of PAHs and OCPs have decreased significantly in the last ten years, while those of PAEs and Es had an upward trend compared with the previous studies. BPA still remained at a moderately high level, as it was ten years ago. The risks of the PAEs in all of the sample sites, and fluoranthene, benzo[a]anthrene, and benzo[a]pyrene in the Wenyu River sediment, were relatively high. These results supplemented the database of toxic organics’ concentration levels in the sediments of Beijing rivers.

Open image in new window

     

Distribution of Juvenile,subadult and adult Scylla serrata (Crustacea: Portunidae) on tidal flats in Australia

In Deception Bay, northern Australia, during 1979–1981, a study was made of the distribution of Scylla serrata (Forskal) in an area having a broad intertidal zone. Juveniles (20 to 99 mm carapace width) were resident in the mangrove zone, remaining there during low tide. The majority of subadult crabs (100 to 149 mm) migrated into the intertidal zone to feed at high tide and retreated to subtidal waters at low tide. Adults (150 mm and larger) were caught mainly subtidally and only small numbers were captured in the intertidal at high tide. Few crabs were captured in the coolest months (May to August). Adults were captured on the flats mainly in the warmest months (January to April), but subadults could be captured over the entire summer (September to March). Juveniles were found in the upper intertidal throughout the year.     

Prenatal diagnosis of NADH:ubiquinone oxidoreductase deficiency

NADH:ubiquinone oxidoreductase (complex I of the mitochondrial respiratory chain) deficiency is a severe disorder with an often early fatal outcome. Prenatal diagnosis for complex I defects currently relies mainly on biochemical assays of complex I in fetal tissues such as chorionic villi (CV), and is only in a minority of cases possible by means of mutational analysis of nuclear-encoded genes of complex I. We report on our experience to date with prenatal diagnosis in pregnancies at risk for complex I deficiency. We measured complex I activity in native CV and/or cultured CV in 23 pregnancies in 15 families. In accordance with the results of the investigations in CV, 15 children were born clinically unaffected. Two prenatally diagnosed unaffected fetuses and two prenatally diagnosed affected fetuses were lost prematurely with spontaneous or provoked abortions, respectively. Two affected children were born (prenatally found to be affected). In two pregnancies a discrepancy between native and cultured cells was found. We conclude that prenatal diagnosis for complex I deficiency can be reliably performed. Pitfalls were encountered in using cultured CV as a result of maternal cell contamination (MCC). Future research on pathogenic nuclear mutations underlying complex I deficiency will extend the possibilities for prenatal diagnosis at the molecular level. Copyright © 2001 John Wiley & Sons, Ltd.