Phosphorus and sulfur in a tropical soil and their effects on growth and selenium accumulation in Leucaena leucocephala (Lam.) de Wit

Selenium (Se) is an essential metalloid element for mammals. Nonetheless, both deficiency and excess of Se in the environment are associated with several diseases in animals and humans. Here, we investigated the interaction of Se, supplied as selenate (Se⁺⁶) and selenite (Se⁺⁴), with phosphorus (P) and sulfur (S) in a weathered tropical soil and their effects on growth and Se accumulation in Leucaena leucocephala (Lam.) de Wit. The P-Se interaction effects on L. leucocephala growth differed between the Se forms (selenate and selenite) supplied in the soil. Selenate was prejudicial to plants grown in the soil with low P dose, while selenite was harmful to plants grown in soil with high P dose. The decreasing soil S dose increased the toxic effect of Se in L. leucocephala plants. Se tissue concentration and total Se accumulation in L. leucocephala shoot were higher with selenate supply in the soil when compared with selenite. Therefore, selenite proved to be less phytoavailable in the weathered tropical soil and, at the same time, more toxic to L. leucocephala plants than selenate. Thus, it is expected that L. leucocephala plants are more efficient to phytoextract and accumulate Se as selenate than Se as selenite from weathered tropical soils, for either strategy of phytoremediation (decontamination of Se-polluted soils) or purposes of biofortification for animal feed (fertilization of Se-poor soils).

     

Urban wild meat consumption and trade in central Amazonia

The switch from hunting wild meat for home consumption to supplying more lucrative city markets in Amazonia can adversely affect some game species. Despite this, information on the amounts of wild meat eaten in Amazonian cities is still limited. We estimated wild meat consumption rates in 5 cities in the State of Amazonas in Brazil through 1046 door-to-door household interviews conducted from 2004 to 2012. With these data, we modeled the relationship between wild meat use and a selection of socioeconomic indices. We then scaled up our model to determine the amounts of wild meat likely to be consumed annually in the 62 urban centers in central Amazonia. A total of 80.3% of all interviewees reported consuming wild meat during an average of 29.3 (CI 11.6) days per year. Most wild meat was reported as bought in local markets (80.1%) or hunted by a family member (14.9%). Twenty-one taxa were cited as consumed, mostly mammals (71.6%), followed by reptiles (23.2%) and then birds (5.2%). The declared frequency of wild meat consumption was positively correlated with the proportion of rural population as well as with the per capita gross domestic product of the municipality (administrative divisions) where the cities were seated. We estimated that as much as 10,691 t of wild meat might be consumed annually in the 62 urban centers within central Amazonia, the equivalent of 6.49 kg per person per year. In monetary terms, this amounts to US$21.72 per person per year or US$35.1 million overall, the latter figure is comparable to fish and timber production in the region. Given this magnitude of wild meat trade in central Amazonia, it is fundamental to integrate this activity into the formal economy and actively develop policies that allow the trade of more resilient taxa and restrict trade in species sensitive to hunting.     

Actions of 17β-estradiol on carbohydrate metabolism in liver,gills, and brain of gilthead sea bream Sparus auratus during acclimation to different salinities

The influence of gonadal maturation on seawater adaptability at the metabolic level was assessed in the euryhaline teleost Sparus auratus by treatment of immature fish with 17ß-estradiol (E₂) followed by acclimation to different environmental salinities. Fish were injected with coconut oil alone (sham) or containing E₂ (10 µg g^–1 body weight) and maintained in seawater (40). After 5 days, fish from oil- and E₂-implanted groups were sampled. At the same time, fish of both groups were transferred to brackish water (5, hypoosmotic test), seawater (40, transfer test), and hypersaline water (55, hyperosmotic test); 4 days after transfer (9 days post-implant) all groups were sampled. Data obtained from liver suggest that E₂ treatment produced effects comparable to those already reported in literature, including decreased glycogen levels, increased glycolytic potential, and decreased gluconeogenic potential. This, together with the fact that many changes displayed in shams among salinities disappeared in E₂-treated fish allow us to suggest that the response of liver carbohydrate metabolism to osmotic acclimation is exceeded by the response elicited by E₂ treatment. In gills, E₂ treatment produced increased lactate levels, decreased capacity for use of exogenous glucose, and decreased the potential of the pentose phosphate pathway. These findings suggest that the energy demand occurring in gills during osmotic acclimation should be increasingly fuelled by substrates other than exogenous glucose. Finally, data obtained in brain of E₂-treated fish suggest a lower necessity of exogenous glucose, increased lactate levels, and decreased glycolytic potential.Communicated by S.A. Poulet, Roscoff     

Pigment composition and size distribution of phytoplankton in a confined Mediterranean salt marsh ecosystem

Pigment composition and size distribution of phytoplankton were analysed in a group of Mediterranean salt marshes, where hydrology is dominated by sudden inputs during sea storms, followed by long periods of confinement. These marshes are characterized by a low inorganic–organic nutrient ratio, and inorganic nitrogen is especially scarce due to denitrification. Nutrients were the main factor affecting phytoplankton biomass, while zooplankton grazing did not control either phytoplankton community composition, or their size distribution. The relative abundance of the different phytoplankton groups was analysed by correspondence analysis using the pigment composition measured by high-performance liquid chromatography (HPLC) and analysed with the CHEMTAX programme. In this analysis, phytoplankton pigment composition was correlated with two nutrient gradients. The first gradient was the ratio of nitrate–total nitrogen (TN), since the different phytoplankton groups were distributed according to their eco–physiological differences in nitrogen uptake. The second gradient was correlated with total nutrient loading. Biomass size distributions frequently showed a lack of intermediate sized nanophytoplankton (2.5–4 μm in diameter), and the importance of this lack of intermediate sizes correlated with dinoflagellate biomass. These results suggested that in confined environments, where nutrients are mainly in an organic form, dinoflagellates take advantage of their mixotrophy, by competing and grazing on smaller phytoplankters simultaneously.     

Reproductive conflict in animal societies: hierarchy length increases with colony size in queenless ponerine ants

Dominance interactions determine reproductive status in many animal societies, including many cooperatively breeding vertebrates and eusocial Hymenoptera without queen-worker dimorphism. Typically, the dominant individual monopolises reproduction, and subordinates behave like helpers. In Dinoponera queenless ants, workers are totipotent females and can potentially reproduce, yet only the top-ranking worker actually reproduces. Individual workers ranked immediately below the dominant breeder worker (gamergate) are hopeful reproductives. Whether or not a worker benefits from joining the hierarchy of high-ranking workers depends on the trade-off between the probability of becoming dominant and reproducing directly, and the colony-level cost of an additional lazy high ranker. Inclusive fitness models predict that the length of the dominance hierarchy depends on relatedness, colony size, and the linearity of the hierarchy. Here, we test the effect of colony size by comparing hierarchy length among three species that differ in colony size (Dinoponera australis: median=14 workers, quartiles=10 and 19 workers; D. gigantea: median=41, quartiles=33 and 74; D. quadriceps: median=78, quartiles=55 and 90). Although difficulties in defining where the hierarchy ends hamper comparisons, the results are in broad agreement with the predictions. Hierarchies are close to the predicted lengths and are longer in species with larger colonies (one, three and three workers in the three species in order from smallest to largest colony vs two, three and four predicted). These conclusions are further supported by determining Kokko and Lindström's λ index of skew, which is smaller (i.e. characteristic of a longer hierarchy) in species with larger colonies.     

广州市菜园土和蔬菜中镉含量水平及污染评价

探讨了广州市各区、市菜园土和蔬菜中的重金属Cd污染状况,测定了95份土壤样品以及292份蔬菜样品中的镉含量,并对广州市各区市菜园土中Cd污染程度进行了评价。研究结果表明,不同种类的蔬菜中,叶菜类对重金属Cd的吸收和富集较高,瓜豆类较低。蔬菜中重金属Cd的含量受土壤中Cd含量的影响。与国内其它城市相比,广州市蔬菜地土壤中的Cd平均含量处于较高水平;蔬菜则以海珠、番禺两区Cd含量相对较高,高于其它城市的含量水平。     

Population genetics of the swimming crab<Emphasis Type="Italic"> Callinectes bellicosus</Emphasis> (Brachyura: Portunidae) from the eastern Pacific Ocean

The population genetics and historical demography of the swimming crab Callinectes bellicosus from the eastern Pacific were assessed using mitochondrial DNA (mtDNA) sequences from portions of the cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb) genes. Analysis of molecular variance of sequence data from crabs collected from nine localities, ranging from the upper to lower Gulf of California and the outer coast of the Baja California peninsula, revealed an absence of population structure, suggesting a high level of gene flow over a wide geographic area. Maximum-likelihood estimates of long-term effective population size obtained with the program FLUCTUATE, in addition to highly significant values obtained from neutrality tests (Tajimas D, Fu and Lis D, and Fus F_S) and mismatch analysis, are consistent with a population expansion dating to the Pleistocene epoch. Phylogenetic analysis of C. bellicosus sequences using both neighbor-joining and Bayesian methods revealed a widely distributed subclade (clade II) cryptically embedded at low frequency in the main (clade I) population. Although sequence divergence between the two clades was low (1.1% COI; 0.6% Cytb), statistical support for the split was high. The Kimura-2-parameter genetic distance between C. bellicosus and the sympatric and morphologically similar C. arcuatus was high (d=0.17) and similar to the genetic distance between C. bellicosus and the allopatric C. sapidus from the western Atlantic (d=0.18), suggesting an ancient (Miocene) divergence of C. bellicosus and C. arcuatus.Communicated by P.W. Sammarco, Chauvin     

Effect of complexation by natural phytoplankton exudates on the accumulation of cadmium and copper by the haptophyceae Cricosphaera elongata

The accumulation of cadmium and copper by Cricosphaera elongata is determined in the absence and presence of natural phytoplankton exudates. The concentrations of metals accumulated increase with increasing concentrations of metals in the medium. Organic substances liberated into the culture medium by C. elongata are able to decrease the amounts of metals taken up.     

Molecular receptive ranges of olfactory receptor neurones responding selectively to terpenoids, aliphatic green leaf volatiles and aromatic compounds, in the strawberry blossom weevil Anthonomus rubi

Summary. An important question in insect-plant interactions is which of the numerous plant compounds contribute to the perception of odour qualities in herbivorous insects and are likely to be used as cues in host-searching behaviour. In order to identify which plant-produced volatiles the strawberry blossom weevil Anthonomus rubi detects, we have used electrophysiological recordings from single olfactory neurones linked to gas chromatography and mass spectrometry. We here present 15 receptor neurone types specialised for naturally produced compounds present in the host and nonhost plants and two types for two aggregation pheromone components. The active compounds were terpenoids, aromatic and aliphatic esters, alcohols and aldehydes, some of which are induced by feeding activity of the weevils. The neurones were characterised by a strong response to one or two primary odorants and weaker responses to a few others having similar chemical structure. With one exception, the molecular receptive range of each neurone type was within one chemical group. Enantiomers of linalool separated on a chiral column activated two neurone types with different enantioselectivity. Inhibition by linalool of another neurone type, excited by α-pinene, indicated an additional mechanism for coding the information about this compound. Altogether, detection of 54 compounds by olfactory receptor neurones is shown, of which 40 have been chemically identified in this study. Thus A. rubi has the ability to detect a large number of odorants that may be used in host selection behaviour.