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Zandra C. Deans Anil Biricik Martine De Rycke Gary L. Harton Miroslav Hornak Farrah Khawaja Céline Moutou Jan Traeger-Synodinos Pamela Renwick 《黑龙江环境通报》2023,43(4):506-515
Objective
Genomics Quality Assessment has provided external quality assessments (EQAs) for preimplantation genetic testing (PGT) for 12 years for eight monogenic diseases to identify sub-optimal PGT strategies, testing and reporting of results, which can be shared with the genomics community to aid optimised standards of PGT services for couples.Method
The EQAs were provided in two stages to mimic end-to-end protocols. Stage 1 involved DNA feasibility testing of a couple undergoing PGT and affected proband. Participants were required to report genotyping results and outline their embryo testing strategy. Lymphoblasts were distributed for mock embryo testing for stage 2. Submitted clinical reports and haplotyping results were assessed against peer-ratified criteria. Performance was monitored to identify poor performance.Results
The most common testing methodology was short tandem repeat linkage analysis (59%); however, the adoption of single nucleotide polymorphism-based platforms was observed and a move from blastomere to trophectoderm testing. There was a variation in testing strategies, assigning marker informativity and understanding test limitations, some clinically unsafe. Critical errors were reported for genotyping and interpretation.Conclusion
EQA provides an overview of the standard of preimplantation genetic testing-M clinical testing and identifies areas of improvement for accurate detection of high-risk embryos. 相似文献995.
Abstract: As a contribution to Partners in Flight–Canada, the Canadian Wildlife Service developed a ranking system to help set priorities for landbird species. Two complementary species lists were generated: one with scores for "concern" representing vulnerability and population trend, and one for "responsibility" for regionally characteristic fauna. The concern score gave equal weight to vulnerability, a composite score based on abundance and breadth of range, and population trend, because its purpose was to give early warning of potential problems. Responsibility scores were scale-free and were assigned for the season in which the species is most abundant. Other systems for identifying species of concern may be more appropriate for other jurisdictions or organisms, but the responsibility ranking is widely applicable. This system is a coarse filter that generates preliminary ranks; additional information should be considered in deciding how to allocate scarce resources for conservation. Unrefined results are nonetheless useful for a variety of purposes, as illustrated by the scores for Canadian landbirds. About 25% of Canada's high-responsibility species are also of high concern. These high-concern species are not associated with specific habitats or migration patterns. Conservation of species ranking high on concern and responsibility merits cooperation with the United States and Mexico in particular. The scores identified improved monitoring as a priority conservation activity for Canada. 相似文献
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Summary. Host plant volatiles may be involved in the sexual communication of insects in several ways. In the pheromone-producing sex, these volatiles may affect pheromone production or release and, in the receptive sex, plant volatiles may have a synergistic effect on the attraction to sex pheromone. We conducted three types of experiments to determine if and how plant volatiles are involved in the sexual communication of Lygocoris pabulinus (L.) (Heteroptera: Miridae), the females of which attract males. In a one-choice cylinder bioassay, females were offered to males on two different plants, belonging to different plant families, i.e. potato leaves and goosefoot leaves, to determine if specific plant volatiles were involved in the attraction of males towards females. Females on potato leaves were as attractive as females on goosefoot leaves, but significantly more attractive than females without plant material. The latter result suggests an interaction between females and potato leaves. However, in two-choice flying and walking bioassays, using delta traps in a wind tunnel and a vertical Y-track olfactometer, males were attracted to females irrespective of the presence of potato leaves. This difference in result is probably due to the fact that in the latter assays females were confined with pollen as an alternative food source, while females in the one-choice assay had access to water only, so that they may have suffered from malnutrition. Males in the one-choice assay were also attracted to potato leaves from which females had been removed, indicating that attractive components from females are deposited and adsorbed to the substrate. Plants are probably only indirectly involved in sexual communication, their surface merely functioning as a substrate from which pheromone is released. Males may subsequently be attracted to such plants or substrates. Clean plant material was not attractive to L. pabulinus males, hence plant volatiles alone do not seem to be used by these males as possible mate location cues. 相似文献