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21.
Power matters in closing the phenotyping gap 总被引:1,自引:0,他引:1
Meyer CW Elvert R Scherag A Ehrhardt N Gailus-Durner V Fuchs H Schäfer H Hrabé de Angelis M Heldmaier G Klingenspor M 《Die Naturwissenschaften》2007,94(5):401-406
Much of our understanding of physiology and metabolism is derived from investigating mouse mutants and transgenic mice, and
open-access platforms for standardized mouse phenotyping such as the German Mouse Clinic (GMC) are currently viewed as one
powerful tool for identifying novel gene-function relationships. Phenotyping or phenotypic screening involves the comparison of wild-type control mice with their mutant or transgenic littermates. In our study, we explored
the extent to which standardized phenotyping will succeed in detecting biologically relevant phenotypic differences in mice
generated and provided by different collaborators. We analyzed quantitative metabolic data (body mass, energy intake, and
energy metabolized) collected at the GMC under the current workflow, and used them for statistical power considerations. Our
results demonstrate that there is substantial variability in these parameters among lines of wild-type C57BL/6 (B6) mice from
different sources. Given this variable background noise in mice that serve as controls, subtle phenotypes in mutant or transgenic
littermates may be overlooked. Furthermore, a phenotype observed in one cohort of a mutant line may not be reproducible (to
the same extent) in mice coming from a different environment or supplier. In the light of these constraints, we encourage
researchers to incorporate information on intrastrain variability into future study planning, or to perform advanced hierarchical
analyses. Both will ultimately improve the detectability of novel phenotypes by phenotypic screening.
Carola W. Meyer and Ralf Elvert contributed equally to this work. 相似文献
22.
For more than 30 million years, in early Mesozoic Pangea, ??rauisuchian?? archosaurs were the apex predators in most terrestrial ecosystems, but their biology and evolutionary history remain poorly understood. We describe a new ??rauisuchian?? based on ten individuals found in a single locality from the Middle Triassic (Ladinian) Santa Maria Formation of southern Brazil. Nine articulated and associated skeletons were discovered, three of which have nearly complete skulls. Along with sedimentological and taphonomic data, this suggests that those highly successful predators exhibited some kind of intraspecific interaction. Other monotaxic assemblages of Triassic archosaurs are Late Triassic (Norian-Rhaetian) in age, approximately 10 million years younger than the material described here. Indeed, the studied assemblage may represent the earliest evidence of gregariousness among archosaurs, adding to our knowledge on the origin of a behavior pattern typical of extant taxa. 相似文献
23.
Andreas Musolff Sebastian Leschik Monika Mder Gerhard Strauch Frido Reinstorf Mario Schirmer 《Environmental pollution (Barking, Essex : 1987)》2009,157(11):3069-3077
Based on a monitoring program over the course of a year, we characterize the temporal and spatial distribution of selected micropollutants in an urban watershed within the city of Leipzig, Germany. Micropollutants revealed a ubiquitous presence in untreated and treated wastewater, surface water and groundwater. The loads of 4-nonylphenol in the effluents of the municipal wastewater treatment plant followed a seasonal trend, whereas the loads of all other micropollutants were highly variable and not correlated to seasons. In the surface water, load seasonality of caffeine, galaxolide and tonalide resulted from a rapid removal with increased water temperature. The loads of 4-nonylphenol and of caffeine in the colder months increased when rainfall occurred. In the groundwater, complex spatial and temporal patterns were apparent and were related to varying input, retardation and removal processes. As a consequence, an assessment of micropollutants in urban waters should consider different micropollutants' temporal and spatial variability. 相似文献
24.
Strategies for beneficial use of biosolids in New Zealand and elsewhere are currently focused primarily on land application. The long-term success of these and other strategies is dependent not only on technical factors, but also on their environmental, economic, social and cultural sustainability. This paper briefly reviews the situation with respect to biosolids management in New Zealand, where land application is not yet widespread; the rise in public opposition to land application in the United States; and the biosolids industry's approach to public engagement. We argue that, at least until recently, the industry has misinterpreted the nature and meaning of public opposition and thus substituted public relations for public engagement. We argue that genuine public engagement is necessary and that its purpose cannot be to gain public acceptance for an already-decided-upon strategy. It therefore calls for humility among biosolids managers, including a willingness to open up the framing of 'the problem', to acknowledge areas of uncertainty, and to recognise the role of values in 'technical' decision-making. We then present and analyse an example of the use of the scenario workshop process for public participation in biosolids management policy in Christchurch, New Zealand, and conclude that scenario workshops and related methods represent an opportunity to enhance sustainable waste management when certain conditions are met. 相似文献
25.
Heinz Rüdel Winfried Schröder Karl Theo von der Trenck Gerhard Andreas Wiesmüller 《Environmental science and pollution research international》2009,16(5):486-498
Background Due to the requirements of the EU Water Framework Directive and other legal regulations (e.g., national laws like the German
Federal Soil Protection Act), but also due to the implementation of the new EU chemicals management system REACH, environmental
monitoring will gain increasing importance for the surveillance of environmental quality as well as for the assessment of
chemicals. Against this background, the Work Group on ‘Environmental Monitoring’ of the Division of ‘Environmental Chemistry
and Ecotoxicology’ within the German Chemical Society has compiled a position paper on substance-related environmental monitoring.
Scope Core elements of this position paper are the definitions of important terms like monitoring, exposure monitoring, effect monitoring,
and integrated monitoring. Moreover, temporal and spatial aspects (monitoring of spatial distributions, trend monitoring,
and retrospective monitoring) and their applications are discussed. The main focus of this position paper is the coverage
of aspects which have to be observed for the preparation and implementation of a monitoring program. Essential is the clear
specification of the targets of the monitoring which determine the development of the monitoring concept and its realization,
e.g., if environmental media (compartments) or organisms are most appropriate as samples for the aim of the study. Of course,
also the properties of the target substances are important (e.g., lipophilicity/bioaccumulation as pre-requisite for an exposure
monitoring with organisms). Finally, the monitoring phases of sample planning, sampling, storage and transport of samples,
selection of analytical methods, quality assurance measures as well as reporting are discussed.
Perspectives An important issue for the future is to link the quantification of chemicals in environmental compartments (exposure and pollution
monitoring) more closely to the study of biological effects (effect monitoring) than has been the case up to now. Furthermore,
by inclusion of a spatial differentiation, a comprehensive evaluation of the state of an ecosystem can be obtained and the
relevance of the results improved. Practical examples of monitoring studies which illustrate various aspects covered in this
position paper will be presented in a series of publications by members of the Work Group in the following issues of this
journal.
相似文献
Gerhard Andreas WiesmüllerEmail: |
26.
Exemplifying whole-plant ozone uptake in adult forest trees of contrasting species and site conditions 总被引:1,自引:0,他引:1
Nunn AJ Wieser G Metzger U Löw M Wipfler P Häberle KH Matyssek R 《Environmental pollution (Barking, Essex : 1987)》2007,146(3):629-639
Whole-tree O3 uptake was exemplified for Picea abies, Fagus sylvatica and Larix decidua in stands at high and low altitude and contrasting water availability through sap flow measurement in tree trunks, intrinsically accounting for drought and boundary layer effects on O3 flux. O3 uptake of evergreen spruce per unit foliage area was enhanced by 100% at high relative to low elevation, whereas deciduous beech and larch showed similar uptake regardless of altitude. The responsiveness of the canopy conductance to water vapor and, as a consequence, O3 uptake to soil moisture and air humidity did not differ between species. Unifying findings at the whole-tree level will promote cause-effect based O3 risk assessment and modeling. 相似文献
27.
Langer P Kocan A Tajtáková M Koska J Rádiková Z Ksinantová L Imrich R Hucková M Drobná B Gasperíková D Seböková E Klimes I 《Chemosphere》2008,73(7):1145-1150
In 137 females (F) and 94 males (M) aged 21-35 years from organochlorines (OCs) polluted area (POLL) increased thyroid volume (ThV), prevalence of antibodies to thyroperoxidase (TPOab), thyrotropin receptor (TRab) and of impaired fasting glucose (IFG) was found compared to 116 F and 107 M from background pollution area (BCGR). In F and M from POLL also strikingly increased level of PCBs, DDE and HCB was found. Such findings were compared to the generation of their parents aged 41-55 years consisting of 320F/213M from POLL and 406F/231M from BCGR. However, in spite of strikingly lower level of those OCs in young adults from POLL, they showed about the same prevalence of adverse health signs as the old generation. From such reason 44 young F and 40 young M with lowest PCBs level from POLL were selected to obtain nearly the same PCB level as found in all young F and M from BCGR. In such PCB adjusted groups the prevalence of TPOab, TRab, IFG and increased ThV was still significantly higher than that in all young subjects from BCGR. At the same time, also the level of DDE and HCB in such PCBs adjusted groups was considerably lower. It was concluded that such adverse effects in young adults from POLL possibly did not result from their actual OCs levels, but very likely from their exposure to high OCs levels of their mothers during their prenatal and perinatal life. Thus, the data may be compatible with present views on transgenerational transmission of endocrine disruptors action. 相似文献
28.
Germán A. Kopprio Gerhard Kattner R. Hugo Freije Susana José de Paggi Rubén J. Lara 《Environmental monitoring and assessment》2014,186(5):3139-3148
The seasonal variability of inorganic and organic nutrients and stable isotopes and their relations with plankton and environmental conditions were monitored in Lake Chasicó. Principal component analysis evidenced the strong influence of the river runoff on several biogeochemical variables. Silicate concentrations were controlled by diatom biomass and river discharge. Higher values of nitrate and soluble reactive phosphorus (SRP) indicated agricultural uses in the river basin. Elevated pH values (~9) inhibiting nitrification in the lake explained partially the dominance of ammonium: ~83 % of dissolved inorganic nitrogen (DIN). The low DIN/SRP ratio inferred nitrogen limitation, although the hypotheses of iron and CO2 limitation are relevant in alkaline lakes. Particulate organic matter (POM) and dissolved organic matter (DOM) were mainly of autochthonous origin. The main allochthonous input was imported by the river as POM owning to the arid conditions. Dissolved organic carbon was likely top-down regulated by the bacterioplankton grazer Brachionus plicatilis. The δ13C signature was a good indicator of primary production and its values were influenced probably by CO2 limitation. The δ15N did not evidence nitrogen fixation and suggested the effects of anthropogenic activities. The preservation of a good water quality in the lake is crucial for resource management. 相似文献
29.
Jürgens N Schmiedel U Haarmeyer DH Dengler J Finckh M Goetze D Gröngröft A Hahn K Koulibaly A Luther-Mosebach J Muche G Oldeland J Petersen A Porembski S Rutherford MC Schmidt M Sinsin B Strohbach BJ Thiombiano A Wittig R Zizka G 《Environmental monitoring and assessment》2012,184(2):655-678
The international, interdisciplinary biodiversity research project BIOTA AFRICA initiated a standardized biodiversity monitoring network along climatic gradients across the African continent. Due to an identified lack of adequate monitoring designs, BIOTA AFRICA developed and implemented the standardized BIOTA Biodiversity Observatories, that meet the following criteria (a) enable long-term monitoring of biodiversity, potential driving factors, and relevant indicators with adequate spatial and temporal resolution, (b) facilitate comparability of data generated within different ecosystems, (c) allow integration of many disciplines, (d) allow spatial up-scaling, and (e) be applicable within a network approach. A BIOTA Observatory encompasses an area of 1?km2 and is subdivided into 100 1-ha plots. For meeting the needs of sampling of different organism groups, the hectare plot is again subdivided into standardized subplots, whose sizes follow a geometric series. To allow for different sampling intensities but at the same time to characterize the whole square kilometer, the number of hectare plots to be sampled depends on the requirements of the respective discipline. A hierarchical ranking of the hectare plots ensures that all disciplines monitor as many hectare plots jointly as possible. The BIOTA Observatory design assures repeated, multidisciplinary standardized inventories of biodiversity and its environmental drivers, including options for spatial up- and downscaling and different sampling intensities. BIOTA Observatories have been installed along climatic and landscape gradients in Morocco, West Africa, and southern Africa. In regions with varying land use, several BIOTA Observatories are situated close to each other to analyze management effects. 相似文献
30.
The technique of diffusion gradient in thin films (DGT) for assessing bioavailable metals has not been tested under field
conditions. We assessed the relationships of DGT- and cation exchange resin-membrane-measured concentrations of Cd, Cu, Pb,
and Zn with plant uptake of the metals under greenhouse and field conditions. In the greenhouse, the effective concentrations
of Cu, Pb, and Zn by DGT correlated significantly with uptake by sorghum (Sorghum bicolor), but cation exchange resin-membrane-measured concentrations of Cd, Pb, and Zn did not correlate with sorghum uptake. In
the field, the DGT-measured concentrations of Cd, Pb, and Zn were not linearly related to uptake Cd, Pb, and Zn by lettuce
(Lactuca sativa) except for Cu uptake (r = 0.87, p < 0.05). Similarly, it was only the resin-membrane-extractable Pb that correlated with Pb uptake by lettuce (r = 0.77; p < 0.05). However, fitting non-linear regression models improved the plant metal uptake predictions by DGT-measured bioavailable
Cd, Cu, Pb, and Zn under field conditions. In conclusion, the DGT technique was fairly predictive of bioavailability in the
greenhouse, but not in the field. 相似文献