Über die Bestimmung der Retention radioaktiver Aerosole im menschlichen Atemtrakt

The Science of Nature -     

A rapid lateral flow immunoassay for the detection of fungal alpha-amylase at the workplace

Fungal alpha-amylase is a flour supplement which is added to improve the quality of bakery products. Various studies have shown that exposure to this enzyme is an important risk factor for the development of bakers' allergy and this allergy is reported to be one of the most frequent causes of occupational asthma. A rapid assay was developed to monitor exposure to occupational allergens directly at the workplace. The sensitivity of the developed assay is 0.32 ng amylase mL(-1) in a buffer system with the commercially available alpha-amylase preparation Fungamyl 1600S as the standard. Initial validation tests (n = 33) were performed with airborne and settled dust from an industrial bakery. The new lateral flow immunoassay detected amylase in 22 of the 26 samples regarded as positive in an enzyme immunoassay, and was negative for all seven enzyme immunoassay-negative samples, while the four lateral flow immunoassay-negative/enzyme immunoassay-positive samples all had levels below 2 ng mL(-1). The sensitivity of 2 ng mL(-1) of the amylase lateral flow immunoassay is sufficient for first screening purposes and, therefore, this simple and rapid assay may allow direct on-site demonstration of work-related hazards of bio-allergen exposure. This would be particularly useful in occupational hygiene practice, especially in traditional or small-scale bakeries which lack the technological skills for testing the exposure to respiratory allergens.     

PENN STATE URBAN RUNOFF MODEL TO PINPOINT FLOOD PEAK SOURCE LOCATION1

ABSTRACT: The Penn State Urban Runoff Model, developed in 1976, is described in this paper. Aside from locating infiltration and detention basin operation in an unconventional manner, the model includes a peak flow presentation table which identifies watershed subareas chiefly responsible for the occurrence of flooding conditions at certain points in the watershed. The results of a case study on an urban drainage basin in the Philadelphia area is discussed, and preferred sites for retention ponds are suggested. The simplicity of the Penn State model is pointed out and computer run costs between 10 and 20 percent of the corresponding cards for HEC-I and SWMM are cited.     

Dynamic Cost-Effective Reduction Strategies for Acidification in Europe: An Application to Ireland and the United Kingdom

This paper describes the application of an optimisation model for calculating cost-effective abatement strategies for the reduction of acidification in Europe while taking into account the dynamic character of soil acidification in a number of countries. Environmental constraints are defined in terms of soil quality indicators, e.g., pH, base saturation or the aluminium ion concentration in the soil solution within an optimisation model for transboundary air pollution.We present a case study for Ireland and the United Kingdom. Our results indicate that reduction of sulphur dioxide emission is more cost-effective than that of nitrogen oxides or ammonia. The reduction percentages for sulphur dioxide are highest, for two reasons: (i) marginal sulphur dioxide reduction costs are relatively low compared to marginal reduction costs of nitrogen oxides and ammonia and (ii) sulphur dioxide reduction is more effective in reducing acidification in physical terms than nitrogen oxides or ammonia abatement. Our dynamic analysis shows that a (fast) improvement of soil quality requires high emission reduction levels. These reduction levels are often higher than reduction levels that are typically deduced from the static critical loads approach. Once soil quality targets are reached, in our model, less stringent emission reductions are required to maintain the soil quality at a constant and good target level. Static critical load approaches that ignore dynamic aspects therefore may underestimate the emission reductions needed to achieve predefined soil quality targets.     

Regional scale variability in sediment and nutrient delivery from small agricultural watersheds

Although many studies have pointed out the various controlling factors of sediment and nutrient delivery on a plot or watershed scale, little is known on the spatial variability of sediment and nutrient delivery on a regional scale. This study was conducted to reveal regional variations in sediment-associated nutrient delivery in central Belgium. Sediment deposited in 13 small retention ponds was sampled and analyzed for total phosphorus (TP), K, Mg, and Ca content. The TP content of the sediment deposits varied from 510 to 2001 mg P per kg sediment. Nutrients are predominantly fixed on the very fine sediment fraction (<16 microm), which is the reason why the nutrient trap efficiency of the ponds is only a fraction of the sediment trap efficiency. Average nutrient trap efficiency of the studied ponds varies between 4 and 31%, whereas sediment trap efficiency varies between 10 and 72%. For watersheds ranging from 7 to 4873 ha, sediment yield ranged between 1.2 and 20.6 Mg ha(-1) yr(-1), whereas TP export varied from 1.8 to 39.7 kg ha(-1) yr(-1). The observed spatial variability in nutrient losses is primarily attributed to regional variations in erosion and sediment yield values and to a far lesser degree to the spatial variations in fertilizer application. Redistribution of manure in the framework of an agricultural policy may increase the rate of nutrient delivery by ways of erosion and sediment transport.     

Visualizing different uranium oxidation states during the surface alteration of uraninite and uranium tetrachloride

Low-temperature alteration reactions on uranium phases may lead to the mobilization of uranium and thereby poses a potential threat to humans living close to uranium-contaminated sites. In this study, the surface alteration of uraninite (UO₂) and uranium tetrachloride (UCl₄) in air atmosphere was studied by confocal laser scanning microscopy (CLSM) and laser-induced fluorescence spectroscopy using an excitation wavelength of 408 nm. It was found that within minutes the oxidation state on the surface of the uraninite and the uranium tetrachloride changed. During the surface alteration process U(IV) atoms on the uraninite and uranium tetrachloride surface became stepwise oxidized by a one-electron step at first to U(V) and then further to U(VI). These observed changes in the oxidation states of the uraninite surface were microscopically visualized and spectroscopically identified on the basis of their fluorescence emission signal. A fluorescence signal in the wavelength range of 415–475 nm was indicative for metastable uranium(V), and a fluorescence signal in the range of 480–560 nm was identified as uranium(VI). In addition, the oxidation process of tetravalent uranium in aqueous solution at pH 0.3 was visualized by CLSM and U(V) was fluorescence spectroscopically identified. The combination of microscopy and fluorescence spectroscopy provided a very convincing visualization of the brief presence of U(V) as a metastable reaction intermediate and of the simultaneous coexistence of the three states U(IV), U(V), and U(VI). These results have a significant importance for fundamental uranium redox chemistry and should contribute to a better understanding of the geochemical behavior of uranium in nature. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

ANALYZING URBANIZATION IMPACTS ON PENNSYLVANIA FLOOD PEAKS1

ABSTRACT: The impact of man made change on the hydrology of developing watersheds is frequently measured in terms of the ratio: flood peak after development to flood peak before development over a range of return periods. However, the analysis of urbanization effects on flood frequency presents a vexing problem because of a general lack of flood data in urban areas and also because of nonstationarity in the development process. Clearly, the flood peak ratio depends on the impervious fraction and percent of basin sewered and these factors have been taken into account in recent urban flood peak models. In genral, these models are developed either by: (1) split sample analysis of available annual flood data, or (2) by computer simulation using mathematical watershed models capable of representing man made changes. The present paper discusses the results of work in progress to characterize the impact of urbanization on small developing watersheds in Pennsylvania.