Characterization of fluoride-tolerant halophilic Bacillus flexus NM25 (HQ875778) isolated from fluoride-affected soil in Birbhum District, West Bengal, India

A new Gram-positive, nonpigmented, rod-shaped fluoride-tolerant bacterial strain, NM25, was isolated from waterlogged muddy field soil collected from the fluoride endemic area of Rampurhat II block (average fluoride in water, 4.7 mg/l, and in soil, 1.5 mg/kg) in Birbhum District, West Bengal, India. The study was undertaken to characterize the fluoride-tolerant bacterial isolate, to determine its role in bioaccumulation of fluoride, and to analyze the water and soil quality of the bacterial environment. The isolate was positive for catalase, lipase, urease, protease, oxidase, and H₂S production, but negative for indole production, nitrate reduction, and Vogues–Proskauer test. The organisms were sensitive to recommended doses of ofloxacin, kanamycin, rifampicin, levofloxacin, vancomycin, gatifloxacin, gentamicin, doxycycline, streptomycin, and nalidixic acid but resistant to ampicillin. Based on the phenotypic characteristics, 16S rRNA gene sequence, and phylogenetic analysis, the bacterial isolate NM25 was identified as Bacillus flexus. The G+C content of the 16S rDNA was 53.14 mol%. This strain tolerated up to 20 % (w/v) NaCl in nutrient agar medium and was grown at the pH range 4–12. It reduced fluoride concentration up to 67.45 % and tolerated more than 1,500 ppm of fluoride in brain–heart infusion agar medium.     

Hydrogeomorphic variability due to dam constructions and emerging problems: a case study of Damodar River,West Bengal,India

In the first multipurpose river valley planning of India, the vast resources of Damodar River Basin (DRB) (eastern India) are not only to be envisioned in their entirety but also to be developed in a unified manner where the water, land, and people are simultaneously bounded in a seamless web. Four large dams (Konar, Tilaiya, Maithon, and Panchet), Durgapur barrage, and Tenughat reservoir are built to tamp the flood-prone Damodar River using water resource in an integrated method. The functionality of Damodar fluvial system is controlled by dams, barrage, weirs, sluices, embankments, and canals, maintaining a dynamic equilibrium between fluvial processes and anthropogenic processes. Carrying more than 50 years of legacy, the existing drainage and flood control system of Damodar Valley Corporation has aggravated a number of hydrogeomorphic problems especially in lower DRB, viz. siltation of river bed and reservoirs, uncontrolled monsoonal stream flow, declining carrying capacity of lower course, drainage congestion, low-magnitude annual floods, channel shifting, de-functioned canals, decay of paleochannels, decline of ground water level, and less replenishing of soils with fresh silts. The present paper is mainly tried to investigate the pre-dam and post-dam hydrogeomorphic variability in relation to flood risk and drawbacks of Damodar Valley Multipurpose Project. Specifically, the annual peak flow of Damodar shifts from August to September due to dam construction and reservoir storage. Applying the annual flood series of log Pearson type III distribution, we have estimated post-dam 5-year peak discharge of above 5,300 m³ s^?1 and 100-year flood of above 11,000 m³ s^?1. Due to siltation, the bankfull discharges of sample segments are gradually declined up to 4,011 m³ s^?1, 2,366 m³ s^?1, and 1,542 m³ s^?1, respectively, having recurrence interval of 1.18–3.18 years only. With the regulation of monsoon flow, the standard sinuosity index is gradually increased downstream, having high dominance of hydraulic factors in respect of topographic factors. The upstream section of study area (Rhondia to Paikpara) now shows the dominance of aggradational landforms, braiding, avulsion, high width–depth ratio, breaching of right bank, and valley widening, but downstream of Barsul the phenomena of bank erosion, confined sinuosity, low width–depth ratio, and narrowness are more pronounced.     

Comparison of alkaline industrial wastes for aqueous mineral carbon sequestration through a parallel reactivity study

Thirty-one alkaline industrial wastes from a wide range of industrial processes were acquired and screened for application in an aqueous carbon sequestration process. The wastes were evaluated for their potential to leach polyvalent cations and base species. Following mixing with a simple sodium bicarbonate solution, chemistries of the aqueous and solid phases were analyzed. Experimental results indicated that the most reactive materials were capable of sequestering between 77% and 93% of the available carbon under experimental conditions in four hours. These materials – cement kiln dust, spray dryer absorber ash, and circulating dry scrubber ash – are thus good candidates for detailed, process-oriented studies. Chemical equilibrium modeling indicated that amorphous calcium carbonate is likely responsible for the observed sequestration. High variability and low reactive fractions render many other materials less attractive for further pursuit without considering preprocessing or activation techniques.     

Visible light photocatalysts from low-grade iron ore: the environmentally benign production of magnetite/carbon (Fe3O4/C) nanocomposites

Environmental Science and Pollution Research - Magnetite (Fe3O4) nanoparticles coated with dextrose and gluconic acid possessing both super-paramagnetism and excellent optical properties have been...     

Projected Changes in Discharge in an Agricultural Watershed in Iowa

Our improved capability to adapt to the future changes in discharge is linked to our capability to predict the magnitude or at least the direction of these changes. For the agricultural United States Midwest, too much or too little water has severe socioeconomic impacts. Here, we focus on the Raccoon River at Van Meter, Iowa, and use a statistical approach to examine projected changes in discharge. We build on statistical models using rainfall and harvested corn and soybean acreage to explain the observed discharge variability. We then use projections of these two predictors to examine the projected discharge response. Results are based on seven global climate models part of the Coupled Model Intercomparison Project Phase 5 and two representative concentration pathways (RCPs 4.5 and 8.5). There is not a strong signal of change in the discharge projections under the RCP 4.5. However, the results for the RCP 8.5 point to a stronger changing signal related to larger projected increases in rainfall, resulting in increased trends, in particular, in the upper part of the discharge distribution (i.e., 60th percentile and above). Examination of two hypothetical agricultural scenarios indicates that these increasing trends could be alleviated by decreasing the extent of the agricultural production. We also discuss how the methodology presented in this study represents a viable approach to move forward with the concept of return period for engineering design and management in a nonstationary world.     

Variation of antioxidant biomarkers in the edible oyster Saccostrea cucullata collected from three different water bodies of Sundarbans

Variations of antioxidant biomarkers such as catalase (CAT), superoxide dismutase (SOD) activities and lipid peroxidation (LPO) were studied in edible part of mangrove oyster Saccostrea cucullata, collected from three different water bodies, such as Namkhana (S-I), Frazergaunge (S-II) and Sajnekhali (S-III) of Indian Sundarbans which are exposed to different degrees of anthropogenic activity. The study was conducted for consecutive two years (2010 and 2011) in the respective water bodies. Characteristics of biomarkers in oyster from the two polluted water bodies, i.e. S-I and S-II, are similar in nature in comparison to less polluted site (S-III). The catalase, superoxide dismutase activity and lipid peroxidation in oyster flesh exhibit significant (p<0.01) spatial and temporal variation among the three stations. Oysters from S-I were significantly higher (p<0.05) in antioxidant enzyme activities than oysters from S-III, which differ in their amount of pollution sources. Maximum antioxidant enzymes activity of all collected samples were recorded in pre-monsoon time and decreased in monsoon season. But maximum lipid peroxidation was noted during monsoon followed by post-monsoon and pre-monsoon.