Resolution of DNA linkage discrepancies through analysis of a VNTR locus in a family study of cystic fibrosis

First-trimester prenatal diagnosis of a fetus at 25 per cent risk for cystic fibrosis (CF) was performed by indirect linkage analysis of polymorphic markers using Southern blotting and polymerase chain reaction (PCR) amplification. The results revealed discrepancies in the allelic patterns between the father and the affected child, thereby complicating the prediction of fetal outcome. Analysis of a highly polymorphic VNTR locus within the human retinoblas-toma (RB) gene on chromosome 13 showed that the affected child and the fetus did not have the same biological father, and therefore the affected child could not be used to determine linkage of markers in the father of the fetus. The analysis of VNTR loci can be an effective method of resolving conflicting data during prenatal diagnosis of monogenic diseases.     

NITROGEN AND PHOSPHORUS IN SURFACE WATERS OF THE UPPER COLORADO RWER BASIN1

ABSTRACT: As part of a basinwide water-quality study, nitrogen and phosphorus data for the Upper Colorado River Basin from the Colorado-Utah State line to the Continental Divide were analyzed for spatial distributions, concentrations associated with various land uses, and temporal trends. Nitrogen and phosphorus concentrations generally increased in a downstream direction. Some nutrient concentrations were elevated at some sites in the upper parts of the basin in areas influenced by increasing urbanization. Sites were grouped according to land use and site type, and median nutrient concentrations were compared among groups. Sites within the agricultural areas of the basin generally had the highest concentrations of nitrogen and phosphorus; concentrations for main-stem, tributary, and urbanization sites were slightly lower than for the agricultural sites. Background sites, or sites with minimal land-use impacts, had very low median nutrient concentrations. Several sites with long-term data were analyzed for temporal trends in concentrations. Several statistically significant downward trends of low and moderate magnitude were observed for nitrogen and phosphorus species. No upward trends were observed in the data at any site.     

Isotope applications in environmental investigations part II: Groundwater age dating and recharge processes,and provenance of sulfur and methane

Measurement of the isotopic composition of solids, solutes, gases, and water complement standard hydrogeological investigation techniques by providing information that may not otherwise be obtainable. Groundwater age estimates determined from the decay of radio‐isotopes or from groundwater concentrations of anthropogenic gases such as chlorofluorocarbons (CFCs) and sulfur hexafluoride (SF₆) are used to verify flow regimes and constrain or calibrate hydrologic flow models. Groundwater recharge rates are estimated by measuring the concentrations or activities of a variety of isotopes including ²H, ³H, ¹⁸O, and ³⁶Cl. Excess sulfur causes salinization of water supplies and acidification of precipitation, surface water, and groundwater. The wide range of sulfur isotopic compositions exhibited by different sulfur species and sources allows the application of sulfur isotopes to trace sources and fate of sulfur in the environment. Methane is a ubiquitous gas that has economic value when located in extractable reservoirs. Methane is also a greenhouse gas and is a potential explosion and health hazard when it accumulates in buildings and water distribution systems. The carbon and hydrogen isotopic composition of methane can be used to determine the provenance of methane, distinguishing between thermogenic and biogenic sources. The addition of isotopic analyses to environmental investigations can be a cost‐effective means of resolving intractable issues. © 2003 Wiley Periodicals, Inc.     

Dietary accumulation of UV-absorbing mycosporine-like amino acids (MAAs) by the green sea urchin (Strongylocentrotus droebachiensis)

The bioaccumulation of ultraviolet radiation-absorbing mycosporine-like amino acids (MAAs) by Strongylocentrotus droebachiensis (Müller) was determined in specimens maintained on MAA-rich and MAA-deficient diets. Individuals were fed either the red alga Mastocarpus stellatus (Stackhouse), which has a high concentration of the MAA shinorine (6.98 nmolmg^–1 dry wt), and trace amounts of the MAAs porphyra-334, asterina-330 and palythine, or the brown alga Laminaria saccharina (Lamouroux), which contains no detectable MAAs. Reproductively spent urchins were fed ad libitum during a 9 mo period in 1992 to 1993 until they were once again gravid. To monitor accumulation, samples of urchin tissues and ingested food were taken throughout the 9 mo period from males and females maintained on each diet, and the concentrations of MAAs determined using high performance liquid chromatography. Urchins maintained on a diet of M. stellatus showed an ovarian shinorine concentration (8.33 nmol mg^–1 dry wt) 25 times higher than those fed L. saccharina. There was no change in MAA content of testes or somatic tissues in either feeding group. More than 99% of the MAAs present in an experimental meal of M. stellatus were removed during passage through the gut. Previous studies have inferred dietary accumulation of MAAs by comparing MAAs present in animal tissues with the consumer's potential diet in the field. This is the first study to have monitored MAA accumulation in animal tissues in conjunction with a controlled diet having a known MAA composition. Concentrating MAAs in the ovaries may provide eggs released into the water column with protection from damaging solar ultraviolet radiation.     

Mitigating Greenhouse Gas Emissions from Rice-Wheat Cropping Systems in Asia

The rice-wheat belt comprises nearly 24–27 million ha in South and East Asia. Rice is generally grown in flooded fields whereas the ensuing wheat crop requires well-drained soil conditions. Consequently, both crops differ markedly in nature and intensity of greenhouse gas (GHG) fluxes, namely emission of (1) methane (CH₄) and (2) nitrous oxide (N_2O) as well as the sequestration of (3) carbon dioxide. Wetland rice emits large quantities of CH₄; strategies to CH₄ emissions include proper management of organic inputs, temporary (mid-season) field drainage and direct seeding. As for the wheat crop, the major GHG is N_2O that is emitted in short-term pulses after fertilization, heavy rainfall and irrigation events. However, N_2O is also emitted in larger quantities during fallow periods and during the rice crop as long as episodic irrigation or rainfall result in aerobic-anaerobic cycles. Wetland rice ensures a relatively high content of soil organic matter in the rice-wheat system as compared to permanent upland conditions. In terms of global warming potential, baseline emissions of the rice-wheat system primarily depend on the management practices during the rice crop while emissions from the wheat crop remain less sensitive to different management practices. The antagonism between CH₄ and N_2O emissions is a major impediment for devising effective mitigation strategies in rice-wheat system - measures to reduce the emission of one GHG often intensify the emission of the other GHG.