Evaluation of the gene expression changes in Nile tilapia (<Emphasis Type="Italic">Oreochromis niloticus</Emphasis>) as affected by the bio-removal of toxic textile dyes from aqueous solution in small-scale bioreactor

A laboratory-scale bioremediation unit was designed, built and tested for the bio-removal of several Direct textile dyes. Four experiments were carried out to assess the efficiency of the bioremediation unit using Aspergillus niger fungal strain. Three commonly used Direct dyes and textile dyes mixture (simulated effluent: Direct brown, Direct violet, Direct green) were tested in this study. The strain of A. niger was efficient in the removal of the three Direct dyes. The decolorization percentages of the dyes after 24 h of incubation were 56.2, 51.7, and 95.4% for Direct brown, Direct green, Direct violet dyes, respectively. The percentages increased up to 79.4, 86.4, and 96.7% after 72 h of incubation for the same dyes, respectively. The results also showed that the fungal strain reduced the chemical oxygen demand values of simulated dye effluents from 165 to 564 mg/l with most of the dyes. The assessment of bioremediation products on biomodel was conducted using a fresh water fish. The liver and brain of Nile tilapia were tested to evaluate the expression of genes coding for several proteins related to stress such as metallothioneins (MTs), cytochrome P450 (CYP450), and heat shock proteins (HSPs). To assess the alterations in the gene expression, ten animals from each group were killed after 4 weeks of treatment. The results revealed significant increases in the brain and hepatic mRNA levels of all stress protein genes MT, CYP450, Hsp70a, b, and Hsp47 in the fish groups treated with industrial Direct violet, green, and brown dye water. Exposure of tilapia to bioremediation products after treatment with A. niger fungi reduced the over-expression of the stress protein genes in the brain and liver tissues.     

Annual nutrients budget for the grazed and ungrazed sites of an alpine expanse in North-West Himalaya,India

This work was undertaken to analyze nutrient contents of vegetation in an alpine meadow—Tungnath, North-West Himalaya, India. The study pertains to the uptake, transfer and release of four main macronutrients (organic carbon, total nitrogen, total potassium and total phosphorus) in grazed (exposed to extensive grazing by cattles) and ungrazed (grazing completely prohibited) communities. Mineral concentration was recorded higher for the ungrazed sites compared to the grazed sites, and maximum standing state of nutrients was found in roots. Belowground compartment (roots) contributed maximum share of mineral elements to soil. Litter nutrients release was low because of low microbial activity and continuous removal of phytomass. Observations reveal that there was very little amount of nutrient release from phytomass and vegetation in alpine are very poor source of mineral recycling. Low transfer rate of minerals from one compartment to other is adequate for greater amount of these minerals that are translocated back into the storage organs. A small proportion get removed through rain splash or through the removal of hay during grazing as relatively high release rates in ungrazed sites when compared to grazed sites was observed. This translocation can be considered as an important adaptation in alpine plants for survival during adverse environmental conditions, against all types of biotic pressures and also for regeneration in the forthcoming growing season.     

An assessment of ecosystem services of Corbett Tiger Reserve,India

This paper examines the economic value of selected ecosystem services of Corbett Tiger Reserve, India. The direct cost was derived from secondary sources, and indirect and opportunity costs through socioeconomic surveys. For recreational value the individual approach to travel cost method was used, and to assess carbon sequestration the replacement cost method was used. The maintenance cost of the reserve was estimated as US $2,153,174.3 year⁻¹. The indirect costs in terms of crop and livestock depredation by wild animals ranged from US $2,153,174.3 year⁻¹. The indirect costs in terms of crop and livestock depredation by wild animals ranged from US 2,408 to US $37,958 village⁻¹ over a period of 5 years. The dependence of local communities was for fuel wood (US $37,958 village⁻¹ over a period of 5 years. The dependence of local communities was for fuel wood (US 7,346 day⁻¹), fodder (US $5,290 day⁻¹), small timber, and other nontimber forest products. The recreational value of the reserve was estimated as US $5,290 day⁻¹), small timber, and other nontimber forest products. The recreational value of the reserve was estimated as US 167,619 year⁻¹. With the cost per visitor being US $2.5, the consumers’ surplus was large, showing the willingness of visitors to pay for wildlife recreation. The forests of the reserve mitigate carbon worth US $2.5, the consumers’ surplus was large, showing the willingness of visitors to pay for wildlife recreation. The forests of the reserve mitigate carbon worth US 63.6 million, with an annual flow of US $65.0 ha⁻¹ year⁻¹. The other benefits of the reserve include US $65.0 ha⁻¹ year⁻¹. The other benefits of the reserve include US 41 million through generation of electricity since 1972. The analysis reveals that, though the benefits outweigh costs, they need to be accrued to local communities so as to balance the distribution of benefits and costs.     

Medium optimization for the production of anti-cyanobacterial substances by Streptomyces sp. HJC-D1 using response surface methodology

Bioremediation using isolated anti-cyanobacterial microorganism has been widely applied in harmful algal blooms (HABs) control. In order to improve the secretion of activated anti-cyanobacterial substances, and lower the cost, a sequential optimization of the culture medium based on statistical design was employed for enhancing the anti-cyanobacterial substances production and chlorophyll a (Chl a) removal by Streptomyces sp. HJC-D1 in the paper. Sucrose and KNO₃ were selected as the most suitable carbon and nitrogen sources based on the one-at-a-time strategy method, and sucrose, KNO₃ and initial pH were found as major factors that affected the anti-cyanobacterial ability of the isolated stain via the Plackett–Burman design. Based on the response surface and canonical analysis, the optimum condition of culture medium was obtained at 22.73 g l^-1 of sucrose, 0.96 g l^-1 of KNO₃, and initial pH 8.82, and the Chl a removal efficiency by strain HJC-D1 increased from 63?±?2 % to 78?±?2 % on the optimum conditions.     

Challenges in assessing biological recovery from acidification in Swedish lakes

Since the 1980s, Swedish lakes have in general become less acidified. Assessment of biological recovery is, however, hampered by poor pre-acidification data, confounding effects of climate change, and few lakes with annual sampling of fish and other organisms. Only three critically acidified, but non-limed, lakes had two decades of fish monitoring. The lakes had not yet recovered to pre-industrial chemical targets. Fish had low species richness compared to other organism groups. Roach (Rutilus rutilus) and/or European perch (Perca fluviatilis) were the dominant fish species, and the acid-sensitive roach had been lost from one of the lakes. Calcium decreased, possibly approaching pre-acidification concentrations, but exceeded minimum levels needed to sustain some Daphnia species. High or increasing levels of total organic carbon, likely due to reduced acidification and climate change, might influence the biological communities in unexpected ways, for example, facilitating more frequent occurrence of the invasive algae Gonyostomum semen.     

Formation of indoor nitrous acid (HONO) by light-induced NO<Subscript>2</Subscript> heterogeneous reactions with white wall paint

Gaseous nitrogen dioxide (NO₂) represents an oxidant that is present in relatively high concentrations in various indoor settings. Remarkably increased NO₂ levels up to 1.5 ppm are associated with homes using gas stoves. The heterogeneous reactions of NO₂ with adsorbed water on surfaces lead to the generation of nitrous acid (HONO). Here, we present a HONO source induced by heterogeneous reactions of NO₂ with selected indoor paint surfaces in the presence of light (300 nm?<?λ?<?400 nm). We demonstrate that the formation of HONO is much more pronounced at elevated relative humidity. In the presence of light (5.5 W m^?2), an increase of HONO production rate of up to 8.6?·?10⁹ molecules cm^?2 s^?1 was observed at [NO₂]?=?60 ppb and 50 % relative humidity (RH). At higher light intensity of 10.6 (W m^?2), the HONO production rate increased to 2.1?·?10¹⁰ molecules cm^?2 s^?1. A high NO₂ to HONO conversion yield of up to 84 % was observed. This result strongly suggests that a light-driven process of indoor HONO production is operational. This work highlights the potential of paint surfaces to generate HONO within indoor environments by light-induced NO₂ heterogeneous reactions.     

Relationship between carbon emissions and economic development: case study of six countries

There is much discussion within the sustainable development community regarding climate stabilization and particularly, finding environmentally equitable ways to address emission reductions. Knowing the current level of emission is only one variable in this complex picture. While the rate of emissions is clearly a problem, the overall increase in GHG concentration in the atmosphere is ultimately the main driver of anthropogenic warming. Therefore, it is also important to understand the cumulative emissions, those which have taken us to the current condition. This research presents a case study of six countries to compare the emissions per capita and cumulative emissions during the past 200 years. It is known that carbon emissions are closely related to economic activities, but here we show that some countries have reached per capita emissions plateaus at different levels while others are still rising. Specifically, one approach toward socioeconomic development, in terms of energy–economy, reaches a plateau at 10 Mt carbon per person, which the United Kingdom and South Korea have attained. The US occupies another emission regime at 20 Mt carbon per person. Developing economies such as India and China are considerably below these levels, and unless they follow other integrated economic/environmental solutions, they will continue to increase their per capita emissions during development.