Bioaccumulation of lead, mercury, and cadmium in the greater white-toothed shrew, Crocidura russula, from the Ebro Delta (NE Spain): sex- and age-dependent variation

We quantified bioaccumulation of lead, mercury, and cadmium in bones from 105 greater white-toothed shrews (Crocidura russula) collected at the Ebro Delta, a polluted area, and the Medas Islands, a control site. Lead and mercury levels varied with site, age, and sex, although statistical significances depended on each factor. Globally, shrews from the polluted area exhibited significantly higher concentrations of Pb and Hg. Increment of Pb with age was particularly remarkable in wetland animals and was interpreted in relation to human activities, namely hunting. Unlike males, females from the Ebro Delta maintained low Hg levels, which were associated with gestation and lactation. Cadmium levels did not differ between sites, sexes, or ages. This study provides the first data on heavy metals in mammals from this wetland and suggests that C. russula is a good bioindicator of metal pollution. We concluded that sex and age may represent an important source of variation in the bioaccumulation of these metals in wild populations.     

The impact of UV-B and sulphur- or copper-containing solutions in acidic conditions on chlorophyll fluorescence in selected Ramalina species

Ramalina maciformis and Ramalina lacera were exposed to different solutions and UV-B to seek for alterations in the PSII photosynthetic quantum yield (F(v)/F(m)), in response to chemicals and radiation. For R. maciformis, significant alterations of the F(v)/F(m) ratio occurred only in response to different bisulphite solutions. The F(v)/F(m) ratio decreased most in R. maciformis and R. lacera following exposure to 5 and 1 mM bisulphite, respectively. Significant differences in F(v)/F(m) ratios were observed for R. lacera in response to different solutions and light at different wavelengths, this being synergistic. The PSII system was unaffected by simulated acid rain in both lichens. R. maciformis, in particular, may survive limited acid rain exposure owing to high Ca oxalate accumulation. The F(v)/F(m) ratio decreased most in R. lacera following short-term exposures to CuSO(4), suggesting that this species is more sensitive to Cu ions under acidic conditions.     

Functional mouthpart morphology of the squat lobster Munida sarsi, with comparison to other anomurans

 The mouthparts of Munida sarsi Huus, 1935, including the labrum and paragnaths, are described using LM and SEM, with special attention to the setal composition and its function. We also used high-resolution macrovideo equipment to record the feeding processes and monitor setae in action. This combination of morphological and preliminary behavioral data enabled us to arrive at a more comprehensive understanding of the functional morphology. M. sarsi was found to be an opportunist and capable of handling and eating most kinds of animal tissue. The species can also be a selective deposit feeder, sorting the sediment by quality and not by size. This sorting is performed by the setae on the medial rim of the bases of maxilla 1, maxilla 2 and maxilliped 1. Many of the other mouthpart functions ranging from food detection to ingestion are mapped with comparison to what has been found for other anomurans. Grooming was shown to be a significant function of many mouthparts, especially the endopod of maxilliped 3. In general the mouthparts closest to the mouth have the least complex movements and functional scheme, with the mandibles being able to perform only one movement, i.e. cutting or crunching food objects. Some functions are attributed to the paragnath, the endopod of maxilla 2 and the epipod of maxilliped 1. Received: 3 October 1999 / Accepted: 22 March 2000     

Patterns of time and space utilisation in coelacanths (Latimeria chalumnae), determined by ultrasonic telemetry

Acoustic telemetry was used to examine patterns of activity and space utilisation of coelacanths, nocturnal predators which spend the day in submarine caves. Nine coelacanths (Latimeria chalumnae) were tracked, each for a period of 1 to 16 nights at Grande Comore, West Indian Ocean. Activities lasted on average 9 h, usually starting shortly after sunset and ending before sunrise. Vertically, coelacanths moved up and down at and below cave level by following the bottom contour, mainly between 180 and 400 m depth. The deepest record was 698 m, the shallowest 133 m. Most time was spent between 200 and 300 m depth. Large individuals performed deep excursions to depths below 400 m, usually once per night. The fish spent most time in water temperatures of 15 to 19 °C; they rarely ventured into waters warmer than 22 °C measured at depths shallower than 160 m depth. Horizontally, coelacanths stayed in narrow areas ranging from <1 to 10 km of coastline. Coelacanths are extremely slow drift-hunters with an estimated average swimming speed of 3.2 m min⁻¹, often travelling not more than 3 km per night. They probably take advantage of local upwelling and downwelling and slow currents occurring parallel to the steep slopes. This study shows that coelacanths are inhabitants of the subphotic zone, where they are active mainly below the depth of their daytime refuges. Received: 7 July 1999 / Accepted: 11 February 2000     

Feeding ecology and evolutionary survival of the living coelacanth Latimeria chalumnae

One concept of evolutionary ecology holds that a living fossil is the result of past evolutionary events, and is adapted to recent selective forces only if they are similar to the selective forces in the past. We describe the present environment of the living coelacanth Latimeria chalumnae Smith, 1939 at Grande Comore, western Indian Ocean and report depth-dependent cave distribution, temperature, salinity and oxygen values which are compared to the fish's distribution and its physiological demands. We studied the activity pattern, feeding behaviour, prey abundance and hunting success to evaluate possible links between environmental conditions, feeding ecology and evolutionary success of this ancient fish. Transmitter tracking experiments indicate nocturnal activity of the piscivorous predator which hunts between approximately 200 m below the surface to 500 m depth. Fish and prey density were measured between 200 and 400 m, both increase with depth. Feeding tracks and feeding strikes of the coelacanth at various depths were simulated with the help of video and laser techniques. Along a 9447 m video transect a total of 31 potential feeding strikes occurred. Assuming 100% hunting success, medium-sized individuals would obtain 122 g and large females 299 g of prey. Estimates of metabolic rates revealed for females 3.7 ml O₂ kg⁻¹ h⁻¹ and for males 4.5 ml O₂ kg⁻¹ h⁻¹. Today coelacanths are considered to be a specialist deep-water form and to inhabit, with their ancient morphology, a contemporary environment where they compete with advanced, modern fish. Received: 5 July 1999 / Accepted: 11 November 1999     

Reef coral reproduction in the eastern Pacific: Costa Rica, Panamá and Galápagos Islands (Ecuador). IV. Agariciidae, recruitment and recovery of Pavona varians and Pavona sp.a

The reproductive ecology of two eastern Pacific zooxanthellate coral species was examined as part of a continuing series of studies relating bleaching/mortality events caused by the El Niño–Southern Oscillation disturbance, and is described for study sites in Costa Rica, Panamá, and the Galápagos Islands (Ecuador). This study deals with the sibling agariciid species Pavona varians and Pavona sp.a over a 13?yr period (1985 to 1997). Both Pavona species are broadcast-spawners with some gonochoric, but mostly sequential hermaphroditic colonies. Minimum colony sizes (and ages) at first reproduction were 5?cm (5?yr) and 3?cm (2 to 3?yr), respectively, in P. varians and Pavona sp.a. In the Panamá and Galápagos populations, gonochoric colonies spawn eggs or sperm at least monthly. Six fecundity attributes were not significantly different in the two species, but the eggs of P. varians are white to beige and positively buoyant, and those of Pavona sp.a are dark green and neutrally to negatively buoyant. Eggs of both species lack zooxanthellae. Both species are reproductively active year-round, with maximum activity in the dry season in the nonupwelling Gulf of Chiriquí, and in the wet season in the upwelling Gulf of Panamá. Spawning is predominantly during full moon, and possibly also at new moon at most study sites. Spawning in P. varians and Pavona sp.a is 12?h out of phase, with the former species spawning ～1?h before sunrise and the latter about 1?h after sunset. The fecundity of Pavona spp. at Caño and the Galápagos Islands was much greater (19?900 to 27?900 eggs cm^?2?yr^?1) than at all Panamá sites (14?800 to 19?800 eggs cm^?2?yr^?1). Intraspecific crosses in both species resulted in swimming planula larvae after 25 to 36?h. Recruitment of P. varians was highest in Panamá, moderate in Costa Rica, and nil in the Galápagos Islands, matching, respectively, the contributions of P. varians to the pre-1982/1983 El Niño coral-population abundances in these areas. Recruitment success of P. varians at Uva Island was significantly related to maximum monthly positive sea surface-temperature (SST) anomalies that occurred in the year preceding recruitment over the period 1982 to 1996; recruitment failed when SST anomalies exceeded 1.6 to 1.9?C° during the severe ENSO events of 1982/1983 and 1997/1998.     

Allozyme electrophoresis indicates high gene flow between populations of Holothuria (Microthele) nobilis (Holothuroidea: Aspidochirotida) on the Great Barrier Reef

 Genetic variation in 15 Holothuria (Microthele) nobilis (Selenka, 1867) populations on the Great Barrier Reef was studied at seven polymorphic allozyme loci. Although populations were separated by distances up to 1300 km, there were no apparent restrictions to gene flow (F _ST values were not significantly different from 0) and the maximum Nei's unbiased genetic distance was 0.003. Populations were in Hardy–Weinberg equilibrium at all loci. The estimated maximum sexual input [this is the ratio of the number of sexually produced individuals (N*) to the sample size (N _i )] and the minimum sexual input [this is the ratio of the number of genotypes (N _go ) over the sample size (N _i )] were used as estimators for the amount of asexual reproduction. Both parameters suggested that H. nobilis reproduces solely by sexual means (N*:N _i : = 1; N _go :N _i  = 0.74 to 1). The allozyme data indicated high gene flow between populations, but the possibility that allozyme frequencies may not be at equilibrium means that it was not possible to distinguish whether the patterns reflect present-day dispersal or dispersal that occurred in the past. Received: 27 March 2000 / Accepted: 29 June 2000     

Life under Arctic sea ice – reproduction strategies of two sympagic (ice-associated) amphipod species, Gammarus wilkitzkii and Apherusa glacialis

The reproductive strategies of two gammaridean amphipod species, Gammarus wilkitzkii and Apherusa glacialis, that permanently inhabit the Arctic sea ice were investigated. G. wilkitzkii reaches sexual maturity at an age of 2 years and produces 128 ± 54 eggs fem.⁻¹ yr⁻¹. Mating takes place during fall and winter, and the development of the large eggs (0.60 to 0.80 mm diam.) lasts 6 to 7 months. The sex ratio of G. wilkitzkii was dominated by males in a proportion of 1.5:1. In vivo studies showed that juveniles are released in batches from the brood pouches of the females during April and May. A. glacialis reaches sexual maturity at the age of 1 year and produces 555 ± 151 eggs fem.⁻¹ yr⁻¹. The eggs are between 0.18 and 0.23 mm in diameter, and are the smallest known for gammaridean amphipods. Eggs are kept in packages of two to eight in the brood pouches of females. The sex ratio of A. glacialis was dominated by females in a proportion of 3:1. The high fecundity of both amphipod species, the release of juveniles in batches over a period of time, a high proportion of females (A. glacialis), and an elongated life-span with multiple spawnings (G. wilkitzkii) are discussed as possible adaptations to the specific and highly variable conditions under Arctic sea ice. Received: 29 December 1999 / Accepted: 8 March 2000     

Occurrence of the semi-catadromous European eel Anguilla anguilla in the Baltic Sea

Strontium (Sr) and calcium (Ca) contents in the otoliths of yellow and silver European eels [Anguilla anguilla (L.)] collected from coastal waters of the Baltic Sea and a freshwater lake in Sweden were examined by wavelength dispersive X-ray spectrometry with an electron microprobe. The mean Sr/Ca ratios from elver check to otolith edge were significantly higher for the eels from coastal waters (5.39 ± 1.09‰) than for those from the lake (0.71 ± 0.89‰). The evidence indicates that European eels in the Baltic Sea do not necessarily migrate into freshwater streams during the growth phase. Received: 30 September 1999 / Accepted: 6 April 2000     

RAPD-PCR investigations of systematic relationships among four species of eels (Teleostei: Anguillidae), particularly Anguilla anguilla and A. rostrata

 We examined the genetic identities of 49 individuals of four species of eels in the genus Anguilla Shaw, A. anguilla, A. japonica, A. reinhardti and A. rostrata, using the random(ly) amplified polymorphic DNA (RAPD)-PCR technique. We used 15 random decamer primers. Out of the 22 148 amplification products detected, 454 markers were evaluated using Nei's distance coefficient, two numerical methods (neighbor-joining, UPGMA), and phylogenetic analysis using parsimony (PAUP). Both numerical and parsimony methods were congruent and confirmed the specific status of all four examined Anguilla species. In all dendrograms, all individuals of each of the species clustered together within four closed groups with highly significant bootstrap values (between 97 and 100%). Additionally 1 to 18 species-specific monomorphic diagnostic fragments were detected in three of the species; none were detected in A. anguilla. Mean interspecific genetic distances ranged from 0.384 to 0.559, with the minimum between A. anguilla and A. rostrata and the maximum between A. anguilla and A. reinhardti. Individuals collected at one locality and belonging to a single species did not cluster together, and neither were any diagnostic monomorphic fragments found for individuals of single localities. This probably reflects a random dispersal of larvae in ocean currents before they reach coastal waters. Among all possible species pairs, only the two Atlantic species were consistently clustered in all dendrograms, with highly significant bootstrap values (100%). Additionally, we detected ten diagnostic markers for this pair of eel species. From RAPD data, we suggest a phylogram which was routed by the descending analysis method: A. reinhardti appears to have been the first species to diverge from a putative common ancestor of the four eel species. Later, A. japonica separated from the hypothetical ancestor of the Atlantic species, which then split recently into A. anguilla and A. rostrata. The close relationship of A. anguilla and A. rostrata is indicated by their low genetic distance (GD = 0.384) and a high degree of shared RAPD fragments (SF = 71.2%); this compares with the following means for all other species pairs: ( = 0.531,  = 44.2%). The prerequisites for using RAPD data for the reconstruction of phylogenies are discussed. Received: 2 September 1999 / Accepted: 8 May 2000