N
2O fluxes as a function of incubation time from soil with different available N contents and pH were determined. Cumulative carbon dioxide (CO
2) emissions were measured to indicate soil respiration. A 144-hr incubation experiment was conducted in a slightly acidic agricultural soil (pH
H2O 5.33) after the pH was adjusted to four different values (3.65, 5.00, 6.90 and 8.55). The experiments consisted of a control without added N, and with NH
4+-N and NO
3--N fertilization. The results showed that soil pH contributed significantly to N
2O flux from the soils. There were higher N
2O emissions in the period 0-12 hr in the four pH treatments, especially those enhanced with N-fertilization. The cumulative N
2O-N emission reached a maximum at pH 8.55 and was stimulated by NO
3--N fertilization (70.4 μg/kg). The minimum emissions appeared at pH 3.65 and were not stimulated by NO
3--N or NH
4+-N fertilization. Soil respiration increased significantly due to N-fertilization. Soil respiration increased positively with soil pH (
R2 = 0.98,
P < 0.01). The lowest CO
2-C emission (30.2 mg/kg) was presented in pH 3.65 soils without N-fertilization. The highest CO
2-C emissions appeared in the pH 8.55 soils for NH
4+-N fertilization (199 mg/kg). These findings suggested that N
2O emissions and soil respiration were significantly influenced by low pH, which strongly inhibits soil microbial nitrification and denitrification activities. The content of NO
3--N in soil significantly and positively affected the N
2O emissions through denitrification.
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