The design and implementation of sustainable plant diversity conservation program for alpine meadows and pastures

The paper describes the design and implementation of a plant biodiversity conservation program that was developed under funding and time constraints for diverse ecological, social, and institutional environments. The biodiversity program for alpine meadows and pastures located in the Swiss Canton of the Grisons is used as an example. The design of the sustainable program relied on existing legislation, accounted for limited ecological knowledge and expertise, and considered biodiversity as a common-pool resource. The trend to intensified cultivation of restricted areas required fast action, while the sustainability of the program design had to take into account institutional diversity. Fifteen habitats and plant communities worth conserving were known, and 57 plant species were identified as indicator species for establishing an inventory and for monitoring purposes. A small subset of 16 well known plant species was presented to the farming communities. They were invited to notify the areas in which they observed the presence of these plants. In different regions of the Canton a total number of 39 paraecologists were trained to inspect the areas notified by farmers and to recommend possible incorporation into the Cantonal inventory. This was done once the farmers signed a contract in which they agreed to follow adequate management practices. The farmers received subsidies to compensate for their losses. Communal authorities controlled the fertilizer input and cutting dates, while the paraecologists were trained to monitor plant biodiversity. The program started in 1992 and the initial phase of the inventory was terminated in 1996. At the beginning of 1996, an inventory of 2617.19 ha, most of which are meadows, was taken and managed according to the rules specified in the contract. The program was considered successful because (i) of the size of the area in the inventory, (ii) about 30%of the farmers participated, and (iii) farmers started cultivating previously abandoned farmland.     

Polychlorinated biphenyls in Narragansett Bay surface sediments

Polychlorinated biphenyls (PCBs) were evaluated in 41 surface sediments collected from Narragansett Bay, RI in 1997-1998. Highest concentrations of total PCBs (1760 ng/g) were in rivers at the head of the bay and the values decreased southward toward the mouth of the bay, with elevated concentrations in some of the coves. The PCB levels in approximately 43% of the samples exceeded the effects range median (ERM) guideline [Environ. Manage. 19 (1995) 81] indicating possible adverse biological effects at these stations. Principal component analysis (PCA) of the surface sediment PCBs separated the Taunton River samples from the rest of the samples. This result suggests that this river has a different PCB composition and sources than the other areas investigated. It also showed that this river has a limited influence on other bay stations as the adjacent samples downstream did not have the same chemical signature. Congener ratios derived from the PCA were useful in distinguishing stations that had different sources of PCBs than the bulk of the bay sediments. Although Aroclor 1268 and 1270 accounted for <1% of all PCB production, their major components, CB206 and CB209, account for 3-6% of the CBs in most bay samples. This may reflect more local use of these Aroclor mixtures and/or be indicative of their relative stability, compared to less chlorinated mixtures. Using linear alkyl benzenes (LABs) as a marker for sewage derived PCBs suggested that up to 95% of the PCBs at the most contaminated sites in the Seekonk, Providence, and Taunton Rivers were sewage derived. This analysis also showed that there is a high background level (167 ng/g) of PCBs in the Seekonk and Providence River, while the Taunton River had a relatively low background level (23.7 ng/g). At the furthest stations south in the Providence River, the sewage derived PCBs only accounted for 23% of the total which suggests that PCB associated with sewage particles are rapidly deposited and are therefore not the most significant source of these compounds to the lower reaches of Narragansett Bay.     

Uptake and metabolism of [<Superscript>14</Superscript>C]rinderine and [<Superscript>14</Superscript>C]retronecine in leaf-beetles of the genus <Emphasis Type="Italic"> Platyphora</Emphasis> and alkaloid accumulation in the exocrine defensive secretions

Summary. Sequestration and processing of pyrrolizidine alkaloids (PAs) by leaf beetles of the genus Platyphora were investigated. Tracer experiments with labeled alkaloids were performed with P. eucosma feeding on Koanophyllon panamense (Asteraceae, tribe Eupatorieae). P. eucosma catalyzes the same reactions previously demonstrated for P. boucardi specialized to Prestonia portobellensis (Apocynaceae): (i) epimerization of rinderine to intermedine; (ii) esterification of retronecine yielding insect-specific PAs; (iii) efficient transport of the PAs as free bases into the defensive secretions. P. bella feeding on Tournefortia cuspidata (Boraginaceae) shows the same sequestration behavior and ability to synthesize the specific retronecine esters. P. ligata, a species phylogenetically closely related to the PA adapted species and clustering in the same clade, but feeding on a host plant devoid of PAs, feeds easily on PA treated host-plant leaves, but does not sequester or metabolize PAs. P. kollari a species clustering outside the PA clade refused to feed on its food-plant leaves painted with PAs. The results are discussed in relation to host-plant selection of the PA adapted species and the role of PAs in chemical defense. Received 20 September 2002; accepted 18 November 2002.     

Fate of ingested pyrrolizidine alkaloid N-oxide in the grasshopper Melanoplus sanguinipes

This work was supported by grants of the Deutsche Forschungsgemeinschaft and by the Fonds der Chemischen Industrie to T.H. and by an NSERC Operating grant to M.B.I. We thank C. Theuring foer technical assistance.